Organ Replacement Research Articles

Statement of Retraction: The first Stem Cell-Based Tissue-Engineered Organ Replacement: Implications for Regenerative Medicine and Society.

Statement of Retraction: The first Stem Cell-Based Tissue-Engineered Organ Replacement: Implications for Regenerative Medicine and Society.

Biomechanical considerations of chemical structure and biosafety of current ceramic biomaterials for dentistry: review and outlook of the current state of the art.

Biomaterial refers to any material, non-pharmaceutical or combination of substances of synthetic or natural origin used for any period independently or as part of a system for the purpose of healing, assisting the growth or replacement of tissues, organs or functions of the body. It is an unsustainable material that interacts with biological systems. Before being produced a biomaterial goes through a sequence of steps, which includes: 1) the definition of the problem that the material will be called to solve (treatment, replacement of the instrument, aesthetic reasons); 2) design of the device, composition and control of materials (mechanical properties, toxicity, biological response, corrosion resistance, interaction with proteins, cells and tissues), manufacture, sterilization and standardization; 3) control of the device (in experimental animals, in vitro) clinical studies, studies; and 4) monitor its long-term use in vivo, in vitro and in patients. Research for ceramic materials is evolving and because they can be porous or glassy, they find applications in medicine and biotechnology, as they can be used as fillers, covering materials and scaffolds. Therefore, ceramic biomaterials are widely used not only in the field of orthopedic and maxillofacial surgery but also in dentistry as for dental prostheses. Materials monitoring technologies allow us to monitor the three-dimensional development in space, as well as imperfections or micro-cracks in the ceramic.

Tinjauan Yuridis terhadap Perbuatan Melawan Hukum Pembina Yayasan yang Melakukan Penyalahgunaan Wewenang dalam Pergantian Organ Yayasan

The replacement of Foundation Organs is carried out when the term of office of the foundation organ has expired and is not re-elected or when the foundation organ has committed acts deemed detrimental to the foundation by the foundation founder as conducted based on the foundation founder's meeting. If the foundation meeting is not held in accordance with quorum provisions stipulated in the Foundation Law and the Articles of Association, then the founder must be held responsible for his actions. This study was conducted using normative research methods. The results show that the unlawful act committed by the founder in the replacement of foundation organs is the abuse of authority by not adhering to the quorum provisions of the founder's meeting by 2/3 of the founder members, while the responsibility for the unlawful acts refers to civil liability in accordance by Article 1365 of the Civil Code. The legal consequences of such unlawful acts on the foundation organ include the occurrence of dualism in the management of the foundation, the invalidity of decisions made by the newly appointed organ which was unlawfully appointed, and damages to third parties.

Return to Peritoneal Dialysis after a Hemodialysis Transfer: A Multistate Analysis of the Canadian Organ Replacement Register

Return to Peritoneal Dialysis after a Hemodialysis Transfer: A Multistate Analysis of the Canadian Organ Replacement Register

Research Status and Safety Considerations of Animal-Derived Mesh Products

With the development of the economy and technological progress, more and more animal-derived mesh products are being utilized in the medical field for tissue and organ repair and replacement. Owing to the complexity of their structure and production process, these animal-derived meshes still face several challenges in practical applications, such as insufficient mechanical strength, rapid degradation rates, and the detection of harmful leachable substances. Among these challenges, the production process is a key factor affecting product quality. This paper reviews the key aspects of the production process and quality control for animal-derived meshes in China, offering new insights for the quality control and regulatory oversight of such products.

Legacy and emerging flame retardants in sediments and wastewater treatment plant-derived biosolids

A baseline assessment of legacy and emerging flame retardant chemicals was performed in inland and transitional sediments as well as biosolids emanating from a selection of wastewater treatment plants (WWTPs) in Ireland. A selection of 24 polychlorinated biphenyls (PCBs), polybrominated diphenyl ethers (PBDEs), and chlorinated organophosphate esters (Cl-OPEs) were quantified in: 81 inland and transitional sediment samples collected during 2023; 39 transitional sediments collected between 2018 and 2022; and 21 biosolid samples collected from 7 WWTPs over 4-month intervals in January, May, and September 2023. Highest concentrations of BDE-209 and several Cl-OPEs were detected in both sediment and biosolid samples, while most PCBs and penta-/octa-BDEs were comparatively low. Moderate levels of PBDEs and Cl-OPEs were detected in Irish sediments compared to similar studies conducted internationally. In biosolid samples, levels of BDE-209 were on the higher end of figured reported worldwide while levels of Σ8Cl-OPEs were the highest relative to comparable international studies. PCBs meanwhile are on the lower end of international levels for both biosolids and sediments. Based on available predicted no-effect concentrations (PNECs), the majority of compounds assessed were found to be of low-risk based on their levels in sediments with the exception of TCIPP (Risk Quotient – RQ = 1.354 = high risk) as well as EHDPP, TEHP, PCB-118, and PCB-52 (RQ = 0.948, 0.576, 0.446, and 0.257 respectively = moderate risk). Similar risk assessment could not be performed on contaminants in biosolids, though levels of BDE-209 were on the higher end of figured reported worldwide (avg = 3155 ng/g) while levels of Σ8Cl-OPEs were the highest relative to comparable international studies (avg8 = 3290 ng/g). As the legacy PBDEs and PCBs have been listed as persistent organic pollutants (POPs) and replacement flame retardants such as Cl-OPEs have been flagged by programmes such as human biomonitoring for EU (HBM4EU) and the NORMAN Network as chemicals of emerging concern, continued monitoring of these moderate and high-risk contaminants in sediments, as well as an investigation of potential contamination of the food chain through land-spreading of biosolids on agricultural lands, would be warranted.

Role of Agrin in tissue repair and regeneration: From mechanisms to therapeutic opportunities (Review).

Tissue regeneration is a complex process that involves the recruitment of various types of cells for healing after injury; it is mediated by numerous precise interactions. However, the identification of effective targets for improving tissue regeneration remains a challenge. As an extracellular matrix protein, Agrin plays a critical role in neuromuscular junction formation. Furthermore, recent studies have revealed the role of Agrin in regulating tissue proliferation and regeneration, which contributes to the repair process of injured tissues. An in‑depth understanding of the role of Agrin will therefore be of value. Given that repair and regeneration processes occur in various parts of the human body, the present systematic review focuses on the role of Agrin in typical tissue and highlights the potential signaling pathways that are involved in Agrin‑induced repair and regeneration. This review offers important insight into novel strategies for the future clinical applications of Agrin‑based therapies, which may represent a feasible treatment option for patients who require organ replacement or repair.

Short-term outcomes of asphyxiated neonates depending on requirement for transfer in the first 24 h of life

ImportanceIn neonates with birth asphyxia (BA) and hypoxic-ischemic encephalopathy, therapeutic hypothermia (TH), initiated within six hours, is the only safe and established neuroprotective measure to prevent secondary brain injury. Infants born outside of TH centers have delayed access to cooling. ObjectiveTo compare in-hospital mortality, occurrence of seizures, and functional status at discharge in newborns with BA depending on postnatal transfer for treatment to another hospital within 24 h of admission (transferred (TN) versus non-transferred neonates (NTN)). DesignNationwide retrospective cohort study from a comprehensive hospital dataset using codes of the International Classification of Diseases, 10th modification (ICD-10). Clinical and outcome information was retrieved from diagnostic and procedural codes. Hierarchical multilevel logistic regression modeling was performed to quantify the effect of being postnatally transferred on target outcomes. SettingAll discharges from German hospitals from 2016 to 2021. ParticipantsFull term neonates with birth asphyxia (ICD-10 code: P21) admitted to a pediatric department on their first day of life. ExposuresPostnatal transfer to a pediatric department within 24 h of admission to an external hospital. Main outcomesIn-hospital death; secondary outcomes: seizures and pediatric complex chronic conditions category (PCCC) ≥ 2. ResultsOf 11,703,800 pediatric cases, 25,914 fulfilled the inclusion criteria. TNs had higher proportions of organ dysfunction, TH, organ replacement therapies, and neurological sequelae in spite of slightly lower proportions of maternal risk factors. In TNs, the adjusted odds ratios (OR) for death, seizures, and PCCC ≥ 2 were 4.08 ((95% confidence interval 3.41–4.89), 2.99 (2.65–3.38), and 1.76 (1.52–2.05), respectively. A subgroup analysis among infants receiving TH (n = 3,283) found less pronounced adjusted ORs for death (1.67 (1.29–2.17)) and seizures (1.26 (1.07–1.48)) and inverse effects for PCCC ≥ 2 (0.81 (0.64–1.02)) in TNs. Conclusion and relevanceThis comprehensive nationwide study found increased odds for adverse outcomes in neonates with BA who were transferred to another facility within 24 h of hospital admission. Closely linking obstetrical units to a pediatric department and balancing geographical coverage of different levels of care facilities might help to minimize risks for postnatal emergency transfer and optimize perinatal care.

Possibilities of Bombyx mori (B. mori) biotechnological platform for regenerative medicine

Regenerative medicine aims at changing modern medicine practice by eliminating core reasons of diseases and disorders. Regenerative medicine includes gene therapy, cell therapy and products of tissue engineering that are destined for augmentation, regeneration or replacement of organs, tissues, genes and metabolic processes in the organism. Biomaterials are amongst key components of regenerative medicine on which successful strategies are based.The review of biotechnological methods implemented in the USP and DSP stages with the use of B. mori was made. The methods analysed are aimed at improving quality characteristics and obtaining new kinds of biomaterials to meet the needs of regenerative medicine and biomedicine. The diversity of biotechnological solutions that allow to gain a wide spectrum of biomaterials (incl. derivatives of cocoon shell such as fibroin, sericin and their composites; recombinant derivatives; antimicrobial peptides; modified transgenic silk fibres;transgenic fibres that contain growth factors and peptides; etc.) is a unique basis for the bioindustrial platform on the B. mori basis.

Effect of Epidermal Mucous Secretion of Earthworms and other Selected Organic Substances on Rooting of Semi-Hardwood Cuttings of <em>Citrus aurantifolia</em> and Top Cuttings of <em>Dracaena sanderiana</em>

A recent trend in plant growth and propagation is to use natural substances rather than synthetic plant growth regulators. Two experiments were conducted to find out the effect of epidermal mucous secretion (EMS) of earthworms and other organic substances including Aloe vera gel, coconut water and ripe banana on rooting of semi-hardwood cuttings of Citrus aurantifolia and top cuttings of Dracaena sanderiana compared to commercially available plant growth regulators (PGRs). Eleven treatments were prepared using sole application of EMS solution, Aloe vera gel, coconut water and ripe banana, and mixtures of EMS and other organic substances in two different ratios by allocating equal and more inputs from EMS. The present experiments were set up adopting a completely randomized design. The number of roots per cutting, total root length per cutting (cm), length of the longest root per cutting (cm) and root vigor score were recorded for both plant species where destructive sampling was done at two months after planting for C. aurantifolia and one and two months after planting for D. sanderiana. The number of roots per cutting, length of the longest root per cutting and the root vigor score were significantly greater in the control treatment that used commercially available PGR and the cuttings treated with EMS: coconut water (1:1) for C. aurantifolia suggesting the possibility to us EMS and coconut water mixture to substitute the PGR for root induction in C. aurantifolia. The number of roots, total root length and the length of the longest root per cutting were significantly high in the treatments administrated with Aloe vera gel, ripe banana, and a mixture of EMS: ripe bananas (1:1) together with the PGR at two months after planting for D. sanderiana. Hence, the ingredients listed above could be utilized as alternative organic substances to produce adventurous roots in D. sanderiana top cuttings as same as the PGR. The results of the present study could be extremely valuable for developing a unique organic rooting replacement for the synthetic root inducing materials for C. aurantifolia and D. sanderiana. Nonetheless, additional research is required to validate the results of this study.

Acoustic-holography-patterned primary hepatocytes possess liver functions

Acoustic holography (AH), a promising approach for cell patterning, emerges as a powerful tool for constructing novel invitro 3D models that mimic organs and cancers features. However, understanding changes in cell function post-AH remains limited. Furthermore, replicating complex physiological and pathological processes solely with cell lines proves challenging. Here, we employed acoustical holographic lattice to assemble primary hepatocytes directly isolated from mice into a cell cluster matrix to construct a liver-shaped tissue sample. For the first time, we evaluated the liver functions of AH-patterned primary hepatocytes. The patterned model exhibited large numbers of self-assembled spheroids and superior multifarious core hepatocyte functions compared to cells in 2D and traditional 3D culture models. AH offers a robust protocol for long-term in vitro culture of primary cells, underscoring its potential for future applications in disease pathogenesis research, drug testing, and organ replacement therapy.

Chrononutrition in Critical Illness.

Circadian rhythms in humans are biological rhythms that regulate various physiological processes within a 24-hour time frame. Critical illness can disrupt the circadian rhythm, as can environmental and clinical factors, including altered light exposure, organ replacement therapies, disrupted sleep-wake cycles, noise, continuous enteral feeding, immobility, and therapeutic interventions. Nonpharmacological interventions, controlling the ICU environment, and pharmacological treatments are among the treatment strategies for circadian disruption. Nutrition establishes biological rhythms in metabolically active peripheral tissues and organs through appropriate synchronization with endocrine signals. Therefore, adhering to a feeding schedule based on the biological clock, a concept known as "chrononutrition," appears to be vitally important for regulating peripheral clocks. Chrononutritional approaches, such as intermittent enteral feeding that includes overnight fasting and consideration of macronutrient composition in enteral solutions, could potentially restore circadian health by resetting peripheral clocks. However, due to the lack of evidence, further studies on the effect of chrononutrition on clinical outcomes in critical illness are needed. The purpose of this review was to discuss the role of chrononutrition in regulating biological rhythms in critical illness, and its impact on clinical outcomes.

Bilateral Crosslinking with Glutaraldehyde and 1-Ethyl-3-(3-Dimethylaminopropyl) Carbodiimide: An Optimization Strategy for the Application of Decellularized Human Amniotic Membrane in Tissue Engineering

Introduction. The decellularized human amniotic membrane (dHAM) emerges as a viable 3D scaffold for organ repair and replacement using a tissue engineering strategy. Glutaraldehyde (GTA) and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) can increase the biomechanical properties of dHAM. However, the crosslinking process is associated with biochemical changes and residual toxic materials, dampening the biocompatibility of the dHAM. From a histologic point of view, each side of the amniotic membrane is biologically different. While the dHAM basement membrane side is rich in growth factors, the stromal side of the dHAM contains more connective tissue matrix (e.g., collagen fibers) which supports its biomechanical properties. Biocompatibility and biomechanical properties are two important challenges in the field of materials science. In this study, for the first time, the stromal and basement membrane side are cross-linked with GTA and EDC, respectively, to optimize the biocompatibility of the treated dHAM while sparing the GTA-mediated biomechanical improvements. Methods. Crosslinking was carried out on dHAM in three groups: EDC, GTA and bilateral treatment with EDC&GTA. Mechanical resistance, degradability, and crosslinking measurements were performed on treated dHAM. The viability of mesenchymal stem cells (MSCs) on the scaffolds was evaluated by the MTT assay. The expression levels of surface markers and images of the MSCs were thoroughly studied. Results. The results obtained showed that bilateral treatment of dHAM with EDC and GTA increased mechanical resistance. Similarly, the evaluation of surface markers revealed that bilaterally treated dHAM sustains the stemness and viability of MSCs at a level equal to that achieved with EDC alone. The SEM images indicated that the MSCs maintained adhesion on EDC&GTA-cross-linked dHAM. Conclusion. The current study explores a pioneering treatment of dHAM, a material long recognized for its regenerative properties, in a novel context. This research delves into the utilization of dHAM cross-linked with EDC&GTA, demonstrating its optimized efficacy in tissue engineering. The enhanced crosslinking technique significantly alters the membrane’s properties, amplifying its durability and therapeutic potential. In this novel bilateral treatment strategy (EDC and GTA), improving mechanical properties by GTA on the stromal surface and maintaining the biocompatibility of EDC on the side of the basement membrane of dHAM had been attained together. By investigating the handling and impact of this cross-linked membrane, this study unveils a new approach in leveraging a well-known material through an innovative process, revolutionizing its application in wound care.

Transcriptomic and western blot characterisation of the human CLEFF4 clone, a new rapid cell line replacement for the Caco2 model

The CLEFF4 sub clone from stock late passage Caco2 cells has a unique property of being able to develop polarised cell monolayers with high P-gp expression and tight junctions much quicker than the original cell line. Instead of being useful for transport studies 21–24 days after initiating culture, the CLEFF4 cell line matures in 5–6 days with tight junctions surpassing that of 3 week old Caco2 cells in that time frame [1].This has enabled the CLEFF4 cell line to provide measures of apparent permeability for potential drug candidates, so important for pre-clinical drug development, 4 times faster than the original cell line. RNA samples were collected and analysed at days 4 and 7 of culture over a 3 year period and had full RNA transcriptome analysed by the ranaseq.eu open bioinformatics platform. Protein was also collected from day 4 to day 22 of culture. Differential expression data from the FASTQ files have shown significant differences in expression in multiple genes involved with drug efflux, tight junctions, phase 2 metabolism and growth factors, which have been confirmed from protein determination that may hold the key to understanding accelerated human cell maturation. These gene expression results may be significant for other tissues beyond the gastrointestinal tract, and potentially for accelerated cell growth for the new field of laboratory grown tissues for organ replacement. The data also confirms the different genetic expression in CLEFF4 cells compared to Caco2 and the stable nature of the different expression over many years.

Idiopathic aseptic necrosis of the femoral head in children who are professionally engaged in gymnastics: A literature review

BACKGROUND: Aseptic necrosis of the femoral head in school-age children is a severe, rapidly progressive degenerative and dystrophic disease. A significant proportion of girls aged 10 years with osteonecrosis of the femoral head have been professionally engaged in rhythmic gymnastics. The relationship between professional sports, in particular rhythmic gymnastics, and the development of this pathology and the mechanism of impaired blood flow in the femoral head in such cases remains unclear. The severity of the course and serious consequences of this disease in the form of multidimensional deformities of the femoral head, early arthrosis of the hip joint, and persistent disability, require close attention.
 AIM: To analyze modern world literature data on the etiology, pathomechanics, and features of the course and treatment of idiopathic aseptic necrosis of the femoral head in children professionally engaged in rhythmic gymnastics.
 MATERIALS AND METHODS: A literature search on the problem of idiopathic aseptic necrosis of the femoral head in children professionally engaged in rhythmic gymnastics in the open information databases was conducted in PubMed, Science Direct, and Library with an analysis depth of 20 years.
 RESULTS: The analysis of publications on the osteonecrosis of the femoral head allowed us to talk about the etiological connection of this condition with professional rhythmic gymnastics, namely, high-intensity repetitive loads on the hip joint of a child. Studies using in vivo laser Doppler flowmetry and 3D computer modeling prove the occlusion of blood vessel branches encircling the femur under excessive mechanical stress on the femoral head and potentially unfavorable positions in the hip joint – overextension (hyperextension), external rotation, and abduction.
 CONCLUSIONS: Professional gymnastics can be a risk factor for the development of osteonecrosis of the femoral head. Frequent late disease diagnoses with the development of severe deformity of the femoral head and end-stage coxarthrosis requiring total hip replacement in adolescents determine the need for early identification of the causes of hip pain in children engaged in gymnastics. The findings will help improve treatment results and reduce the number of organ replacement interventions.

Bionics in Medicine: The Future of Organ Replacement

This article delves into the transformative role of bionics in medicine, particularly in the realm of organ replacement. As the demand for organ transplants significantly outweighs supply, bionic technology presents a revolutionary solution. Beginning with a historical overview, the article traces the evolution of bionics from basic prosthetics to sophisticated artificial organs. It then explores the current state of bionic organ replacement, highlighting existing technologies like artificial hearts and synthetic pancreases, and emphasizes their technological underpinnings, including advanced materials, robotics, and artificial intelligence. The discussion also addresses the significant challenges and limitations faced in this field, such as biocompatibility, ethical considerations, and long-term sustainability. Looking forward, the article forecasts future advancements, including biohybrid organs and 3D organ printing, and contemplates their implications for human health and longevity. Ethical and social considerations are also examined, providing a holistic view of the impact of bionic organs in medicine. Concluding on an optimistic note, the article underscores the importance of continued research and innovation in bionics, envisaging a future where organ scarcity is no longer a barrier to saving lives.

Effects of straw returning on photochemical process and imidacloprid degradation in paddy water through a field experiment

Straw returning is an important strategy for effectively using straw resources to improve soil fertility and quality, but the effects of this process on reactive intermediates (RIs) formation in paddy water are not fully explored. In this study, we conducted a field experiment to explore the effects of straw returning on RIs formation including triplet chromophoric dissolved organic matter (3CDOM*), hydroxyl radicals (•OH), and singlet oxygen (1O2) in paddy water. It was found that straw returning considerably increased the steady-state concentration of RIs, and the application of rice straw with 25% and 75% organic replacement ratio increased RIs concentration by 2.52 and 2.25 times, respectively. The optical spectroscopy, Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) analysis and structural equation model showed that straw returning produces abundant humified and aromatic DOM containing fulvic acid, lignin and protein-like substances, which could induce more RIs under irradiation. In addition, straw returning significantly accelerated imidacloprid (IMD) degradation, yielding degradation products that were highly toxic to aquatic animals (i.e., fish and daphnia) but less toxic to aquatic plants (i.e., green algae). This study would offer an innovative viewpoint on the effects of straw returning on the photochemistry of paddy water and the naturally occurring abiotic degradation pathway of organic contaminants.

Bibliometric Analysis of Historical Block Renewal Research at Home and Abroad based on the Scientific Knowledge Map

The renewal of historical blocks plays an extremely important role in the process of urbanization. However, there are few studies focus on the time distribution of the relevant literature corresponding to the renewal of historical blocks and cannot provide a more cutting-edge opinion conclusion. Therefore, this study aims to explore the law of time distribution in the literature study the renewal of historical blocks. This paper uses the scientific knowledge mapping software CiteSpace to systematically visualize the scientific literature in the field of historical block renewal and its many related fields. To start with, the bibliometric analysis of historical block renewal is carried out. This paper makes a bibliometric analysis of the time distribution, keyword distribution, literature release time, mutation keywords, author density, author network, and other data. This paper finds that the literature in the field of historical district renewal in CSSCI and Peking University core journals has been studied more rapidly in the past decade than in previous years. The increase in the number of papers has increased sharply, and the research hotspots have focused on organic renewal and functional replacement. Among them, micro-renewal, place spirit, and industrial sites are the most concerned frontiers. Based on the above results, this paper summarizes the development trend of the main cutting-edge technologies of the renewal of historical blocks and puts forward relevant suggestions for the development of the renewal field of historical blocks in China.

Retraction: Mesenchymal Stromal Cells for Tissue-Engineered Tissue and Organ Replacements.

[This retracts the article DOI: 10.1111/j.1432-2277.2011.01426.x.].

3D printing of gellan-dextran methacrylate IPNs in glycerol and their bioadhesion by RGD derivatives.

The ever-growing need for new tissue and organ replacement approaches paved the way for tissue engineering. Successful tissue regeneration requires an appropriate scaffold, which allows cell adhesion and provides mechanical support during tissue repair. In this light, an interpenetrating polymer network (IPN) system based on biocompatible polysaccharides, dextran (Dex) and gellan (Ge), was designed and proposed as a surface that facilitates cell adhesion in tissue engineering applications. The new matrix was developed in glycerol, an unconventional solvent, before the chemical functionalization of the polymer backbone, which provides the system with enhanced properties, such as increased stiffness and bioadhesiveness. Dex was modified introducing methacrylic groups, which are known to be sensitive to UV light. At the same time, Ge was functionalized with RGD moieties, known as promoters for cell adhesion. The printability of the systems was evaluated by exploiting the ability of glycerol to act as a co-initiator in the process, speeding up the kinetics of crosslinking. Following semi-IPNs formation, the solvent was removed by extensive solvent exchange with HEPES and CaCl2, leading to conversion into IPNs due to the ionic gelation of Ge chains. Mechanical properties were investigated and IPNs ability to promote osteoblasts adhesion was evaluated on thin-layer, 3D-printed disk films. Our results show a significant increase in adhesion on hydrogels decorated with RGD moieties, where osteoblasts adopted the spindle-shaped morphology typical of adherent mesenchymal cells. Our findings support the use of RGD-decorated Ge/Dex IPNs as new matrices able to support and facilitate cell adhesion in the perspective of bone tissue regeneration.