Organ Biomass Research Articles

Ultra- and Mesostructural Response to Salinization in Two Populations of С3–С4 Intermediate Species Sedobassia sedoides

The plants from two populations (P1 and P2) of xero-halophyte Sedobassia sedoides (Pall.) Freitag & G. Kadereit (Chenopodiaceae) with С3–С4 intermediate type of photosynthesis were examined. Morphophysiological parameters were determined: dry biomass of the above-ground organs, maximum quantum yield of photosystem II (PSII), ultra- and mesostructure of the leaf, carbon isotope discrimination (δ13С) in plants grown under normal conditions and exposed to moderate salinization (0 and 200 mM NaCl). By the values of δ13С and efficiency of PSII, we did not detect significant differences between the populations. Under control conditions, the chloroplasts and mitochondria were located in Kranz-like cells of the bundle sheath of both populations in the region adjacent to vascular bundle, which is characteristic of С2 type of photosynthesis (with photorespiratory СО2 concentrating mechanism). P2 plants were notable for a greater volume of Kranz-like cells of the bundle sheath and more numerous, larger chloroplasts and mitochondria than in P1 plants. By structural leaf parameters, P1 plants may be attributed to a proto-Kranz type of photosynthesis (intermediate between С3 and С2 types) and P2 plants to С2 photosynthesis. Under salinization, the accumulation of dry biomass was reduced in both populations but more considerably in P1. The ultrastructure of organelles in both populations showed different responses to salinization, which was especially pronounced in Kranz-like cells of the bundle sheath. In P1 plants, the area of chloroplasts and mitochondria rose, whereas the area and number of chloroplasts under stress did not change in P2 plants but the area and number of mitochondria decreased. In the cells of plants from both populations, signs of degradation were observed (more pronounced in P1 plants); they were shown in chloroplasts twisting and a disturbance of their granal stacking. In P2 plants, Kranz-like cells of the bundle sheath preserved safer organelles but there occurred the cells with complete degradation of a vacuolar type. Thus, intraspecific ultra- and mesostructural differences were detected in plants from two populations of С3–С4 intermediate species S. sedoides, which reflect different stages of development of photorespiratory СО2 concentrating mechanism. Under salinization, the plants from two different populations showed unlike strategies of adaptation on the level of leaf ultra- and mesostructure.

Estimation of Canopy Biomass Components in Paddy Rice from Combined Optical and SAR Data Using Multi-Target Gaussian Regressor Stacking

Crop biomass is a critical variable to make sound decisions about field crop monitoring activities (fertilizers and irrigation) and crop productivity forecasts. More importantly, crop biomass estimations by components are essential for crop growth monitoring as the yield formation of crops results from the accumulation and transportation of substances between different organs. Retrieval of crop biomass from synthetic aperture radar SAR or optical imagery is of paramount importance for in-season monitoring of crop growth. A combination of optical and SAR imagery can compensate for their limitations and has exhibited comparative advantages in biomass estimation. Notably, the joint estimations of biophysical parameters might be more accurate than that of an individual parameter. Previous studies have attempted to use satellite imagery to estimate aboveground biomass, but the estimation of biomass for individual organs remains a challenge. Multi-target Gaussian process regressor stacking (MGPRS), as a new machine learning method, can be suitably utilized to estimate biomass components jointly from satellite imagery data, as the model does not require a large amount of data for training and can be adjusted to the required degrees of relationship exhibited by the given data. Thus, the aim of this study was to estimate the biomass of individual organs by using MGPRS in conjunction with optical (Sentinel-2A) and SAR (Sentinel-1A) imagery. Two hybrid indices, SAR and optical multiplication vegetation index (SOMVI) and SAR and optical difference vegetation index (SODVI), have been constructed to examine their estimation performance. The hybrid vegetation indices were used as input for the MGPRS and single-target Gaussian process regression (SGPR). The accuracy of the estimation methods was analyzed by in situ measurements of aboveground biomass (AGB) and organ biomass conducted in 2018 and 2019 over the paddy rice fields of Xinghua in Jiangsu Province, China. The results showed that the combined indices (SOMVI and SODVI) performed better than those derived from either the optical or SAR data only. The best predictive accuracy was achieved by the MGPRS using SODVI as input (r2 = 0.84, RMSE = 0.4 kg/m2 for stem biomass; r2 = 0.87, RMSE = 0.16 kg/m2 for AGB). This was higher than using SOMVI as input for the MGPRS (r2 = 0.71, RMSE = 1.12 kg/m2 for stem biomass; r2 = 0.71, RMSE = 0.56 kg/m2 for AGB) or SGPR (r2 = 0.63, RMSE = 1.08 kg/m2 for stem biomass; r2 = 0.67, RMSE = 1.08 kg/m2 for AGB). Relatively, higher accuracy for leaf biomass was achieved using SOMVI (r2 = 0.83) than using SODVI (r2 = 0.73) as input for MGPRS. Our results demonstrate that the combined indices are effective by integrating SAR and optical imagery and MGPRS outperformed SGPR with the same input variable for estimating rice crop biomass. The presented workflow will improve the estimation of crops biomass components from satellite data for effective crop growth monitoring.

Effects of nitrogen application level on photosynthate distribution of Korla fragrant pear trees

Using 13C pulsed labeling technique, we examined the biomass and carbon accumulation of different organs as well as the distribution characteristics of 13C assimilate of 6-year-old Korla fragrant pear trees under three nitrogen application levels, i.e., 150, 300, and 450 kg N·hm-2 (marked as N1, N2, and N3, respectively). Results showed that the biomass, carbon accumulation, 13C fixation and leaf assimilation capacity of the whole pear tree increased while root to shoot ratio decreased with increasing nitrogen application. Both biomass and carbon accumulation amount of reproductive organs (i.e., fruits) were the highest under N2 treatment. The 13C content and distribution rate of each organ changed dynamically along with increasing nitrogen application. At the new shoot growing stage, leaves and roots had stronger competitive abilities for photosynthate, with 13C distribution rates being the highest under N1 treatment. During fruit swelling and mature stages, leaves and fruits were more competitive, with 13C content and distribution rate in leaves being the highest under N3 treatment and those in fruits being the highest under N2 treatment. According to the absorption and distribution characteristics of carbon assimilate across organs under the three nitrogen application levels, the optimal nitrogen application level for achieving high fruit yield in the 6-year-old Korla fragrant pear tree orchard is recommended as 300 kg·hm-2 .

What are the differences in yield formation among two cucumber (Cucumis sativus L.) cultivars and their F1 hybrid?

To elucidate the mechanisms underlying the differences in yield formation among two parents (P1 and P2) and their F1 hybrid of cucumber, biomass production and whole source–sink dynamics were analyzed using a functional–structural plant model (FSPM) that simulates both the number and size of individual organs. Observations of plant development and organ biomass were recorded throughout the growth periods of the plants. The GreenLab Model was used to analyze the differences in fruit setting, organ expansion, biomass production and biomass allocation. The source–sink parameters were estimated from the experimental measurements. Moreover, a particle swarm optimization algorithm (PSO) was applied to analyze whether the fruit setting is related to the source–sink ratio. The results showed that the internal source–sink ratio increased in the vegetative stage and reached a peak until the first fruit setting. The high yield of hybrid F1 is the compound result of both fruit setting and the internal source–sink ratio. The optimization results also revealed that the incremental changes in fruit weight result from the increases in sink strength and proportion of plant biomass allocation for fruits. The model-aided analysis revealed that heterosis is a result of a delicate compromise between fruit setting and fruit sink strength. The organ-level model may provide a computational approach to define the target of breeding by combination with a genetic model.

Elevated CO2 offsets the alteration of foliar chemicals (n-icosane, geranyl acetate, and elixene) induced by elevated O3 in three taxa of O3-tolerant eucalypts

Eucalypts are important forest resources in southwestern China, and may be tolerant to elevated ground-level ozone (O3) concentrations that can negatively affect plant growth. High CO2 may offset O3-induced effects by providing excess carbon to produce secondary metabolites or by inducing stomatal closure. Here, the effects of elevated CO2 and O3 on leaf secondary metabolites and other defense chemicals were studied by exposing seedlings of Eucalyptus globulus, E. grandis, and E. camaldulensis × E. deglupta to a factorial combination of two levels of O3 (< 10 nmol mol−1 and 60 nmol mol−1) and CO2 (ambient: 370 μmol mol−1 and 600 μmol mol−1) in open-top field chambers. GC-profiles of leaf extracts illustrated the effect of elevated O3 and the countering effect of high CO2 on compounds in leaf epicuticular wax and essential oils, i.e., n-icosane, geranyl acetate and elixene, compounds known as a first-line defense against insect herbivores. n-Icosane may be involved in tolerance mechanisms of E. grandis and the hybrid, while geranyl acetate and elixene in the tolerance of E. globulus. Elevated O3 and CO2, singly or in combination, affected only leaf physiology but not biomass of various organs. Elevated CO2 impacted several leaf traits, including stomatal conductance, leaf mass per area, carbon, lignin, n-icosane, geranyl acetate and elixene. Limited effects of elevated O3 on leaf physiology (nitrogen, n-icosane, geranyl acetate, elixene) were commonly offset by elevated CO2. We conclude that E. globulus, E. grandis and the hybrid were tolerant to these O3 and CO2 treatments, and n-icosane, geranyl acetate and elixene may be major players in tolerance mechanisms of the tested species.

Estimating aboveground and organ biomass of plant canopies across the entire season of rice growth with terrestrial laser scanning

Non-destructive and accurate estimation of crop biomass is crucial for the quantitative diagnosis of growth status and timely prediction of grain yield. As an active remote sensing technique, terrestrial laser scanning (TLS) has become increasingly available in crop monitoring for its advantages in recording structural properties. Some researchers have attempted to use TLS data in the estimation of crop aboveground biomass, but only for part of the growing season. Previous studies rarely investigated the estimation of biomass for individual organs, such as the panicles in rice canopies, which led to the poor understanding of TLS technology in monitoring biomass partitioning among organs. The objective of this study was to investigate the potential of TLS in estimating the biomass for individual organs and aboveground biomass of rice and to examine the feasibility of developing universal models for the entire growing season. The field plots experiments were conducted in 2017 and 2018 and involved different nitrogen (N) rates, planting techniques and rice varieties. Three regression approaches, stepwise multiple linear regression (SMLR), random forest regression (RF) and linear mixed-effects (LME) modeling, were evaluated in estimating biomass with extensive TLS and biomass data collected at multiple phenological stages of rice growth across the entire season. The models were calibrated with the 2017 dataset and validated independently with the 2018 dataset.The results demonstrated that growth stage in LME modeling was selected as the most significant random effect on rice growth among the three candidates, which were rice variety, growth stage and planting technique. The LME models grouped by growth stage exhibited higher validation accuracies for all biomass variables over the entire season to varying degrees than SMLR models and RF models. The most pronounced improvement with a LME model was obtained for panicle biomass, with an increase of 0.74 in R2 (LME: R2 = 0.90, SMLR: R2 = 0.16) and a decrease of 1.15 t/ha in RMSE (LME: RMSE =0.79 t/ha, SMLR: RMSE =2.94 t/ha). Compared to SMLR and RF, LME modeling yielded similar estimation accuracies of aboveground biomass for pre-heading stages, but significantly higher accuracies for post-heading stages (LME: R2 = 0.63, RMSE =2.27 t/ha; SMLR: R2 = 0.42, RMSE =2.42 t/ha; RF: R2 = 0.57, RMSE =2.80 t/ha). These findings implied that SMLR was only suitable for the estimation of biomass at pre-heading stages and LME modeling performed remarkably well across all growth stages, especially for post-heading. The results suggest coupling TLS with LME modeling is a promising approach to monitoring rice biomass at post-heading stages at high accuracy and to overcoming the saturation of canopy reflectance signals encountered in optical remote sensing. It also has great potential in the monitoring of other crops in cloud-cover conditions and the instantaneous prediction of grain yield any time before harvest.

Different rootstocks decrease the cadmium accumulation of the post-grafting generation of Brassica juncea var. megarrhiza

ABSTRACTA pot experiment was designed to assess the effects of different rootstocks on cadmium (Cd) accumulation and the growth of the post-grafting generations of Brassica juncea var. megarrhiza. Four treatments, including ungrafted and B. juncea var. megarrhiza as the scion grafted on the rootstocks of Brassica napus, Brassica chinensis and Brassica campestris respectively, were analysed. The three grafting treatments decreased the content of Cd in the first post-grafting generations of B. juncea var. megarrhiza but increased the length of roots, tuberous roots and leaves, and tuberous root diameters, as well as the biomass of the various organs and edible parts. In addition, the grafted rootstocks could enhance the level of photosynthetic pigments and the activity of peroxidase but reduce the activities of superoxide dismutase and catalase and the soluble protein content of the first post-grafting generations of B. juncea var. megarrhiza. The different rootstocks grafts could also decrease the available concentration of Cd in the soil but increase the soil pH. Therefore, the grafting could decrease the accumulation of cadmium in the post-grafting generation of B. juncea var. megarrhiza, and the graft on B. campestris was the most effective.

Ecological Differentiation between Invasive and Native Species of the Genus Epilobium in Riparian Ecosystems Is Associated with Plant Functional Traits

The biomass allocation, leaf traits, and the CO2 assimilation rate in invasive Epilobium adenocaulon Hausskn and E. pseudorubescens A.K. Skvortsov and indigenous E. palustre L. were studied in the Middle Urals. E. adenocaulon had the maximum height, and E. palustre had the largest leaf canopy diameter. The most compact in linear sizes species E. pseudorubescens was characterized by the lowest biomass. The pattern of organ biomass allocation in invasive E. adenocaulon and E. pseudorubescens corresponded to plants with a ruderal strategy, which was expressed in a low proportion of roots and a large proportion of generative organs. Among the studied species, E. adenocaulon was characterized by the largest the stem mass ratio. The indigenous E. palustre had a large proportion of roots and leaves and a smaller proportion of generative organs, which indicated the stress-tolerant properties of this species. In addition to the pattern of biomass allocation, E. palustre differed from the invasive species in a large (30% on average) amount of chlorophyll and 1.2 times less carotenoid content, as well as low photosynthetic activity of chlorophyll. The rate of CO2 uptake per unit leaf area was the greatest in E. adenocaulon, which had thick leaves with a large leaf mass per area (LMA), exceeding that in species E. palustre and E. pseudorubescens with thin leaves with lower LMA by 1.8 and 1.6 times, respectively. A negative correlation between the CO2 assimilation rate per 1 g of dry weight and leaf density (LD) (r = –0.78, p < 0.001) predetermined the maximum value of the photosynthesis rate in E. pseudorubescens, which had the least dense leaves. The analysis of the general dataset of the functional traits indicated that the studied species of the Epilobium genus had different ecological strategies: E. palustre—S (stress tolerant), E. adenocaulon—CR (competitive-ruderal strategy), and E. pseudorubescens—R (ruderal species). It is concluded that invasive species do not have a direct negative effect on E. palustre, even being characterized by distinct ecological properties compared to native species. However, owing to the CR strategy, E. adenocaulon is able to invade riparian plant communities when they are affected by disturbing factors, which may lead to a decrease or to disappearance of the indigenous stress-tolerant species E. palustre and thus to its replacement in disturbed communities by the more competitive invasive species E. adenocaulon.

Changes in the Phenotype of Winter Wheat Varieties Released Between 1920 and 2016 in Response to In-Furrow Fertilizer: Biomass Allocation, Yield, and Grain Protein Concentration.

Plant breeding has increased the yield of winter wheat (Triticum aestivum L.) over decades, and the rate of genetic gain has been faster under high fertility in some countries. However, this response is not universal, and limited information exists on the physiological traits underlying the interaction between varieties and fertilization. Thus, our objectives were to identify the key shifts in crop phenotype in response to selection for yield and quality, and to determine whether historical and modern winter wheat varieties respond differently to in-furrow fertilizer. Factorial field experiments combined eight winter wheat varieties released between 1920 and 2016, and two fertilizer practices [control versus 112 kg ha-1 in-furrow 12 -40-0-10-1 (N-P-K-S-Zn)] in four Kansas environments. Grain yield and grain N-removal increased nonlinearly with year of release, with greater increases between 1966 and 2000. In-furrow fertilizer increased yield by ~300 kg ha-1 with no variety × fertility interaction. Grain protein concentration related negatively to yield, and the residuals of this relationship were unrelated to year of release. Yield increase was associated with changes in thermal time to critical growth stages, as modern varieties had shorter vegetative period and longer grain filling period. Yield gains also derived from more kernels m-2 resultant from more kernels head-1. Historical varieties were taller, had thinner stems, and allocated more biomass to the stem than semidwarf varieties. Yield gains resulted from increases in harvest index and not in biomass accumulation at grain filling and maturity, as shoot biomass was similar among varieties. The allometric exponent (i.e., the slope between log of organ biomass and log of shoot biomass) for grain increased with, and for leaves was unrelated to, year of release. The ability of modern varieties to allocate more biomass to the kernels coupled to an early maturity increased grain yield and grain N-removal over time. However, increases in grain yield were greater than increases in grain N-removal, reducing grain protein concentration in modern varieties.

Phosphorus Application Improves the Cotton Yield by Enhancing Reproductive Organ Biomass and Nutrient Accumulation in Two Cotton Cultivars with Different Phosphorus Sensitivity

Phosphorus (P) plays a pivotal role in cotton by enhancing the reproductive growth and yield formation. Cotton cultivars vary greatly in response to P availability, especially under P-deficient conditions. So, we hypothesized that the increasing P level promotes the reproductive growth in cotton cultivars varying with P sensitivity. For this, two cotton cultivars, Lu-54 (sensitive to low P) and Yuzaomian-9110 (tolerant to low P), in response to three different P levels (P0: 0 (control), P1: 100, and P2: 200 kg P2O5 ha−1) were studied at 39, 52, 69, 83, and 99 days after transplanting during 2017 and 2018. The results revealed that the seed cotton yield was improved in P1 and P2 treatments by 23.9%–34.5% and 30.8%–52.3% in Lu-54, and 16.6%–25.6% and 20.6%–38.5% in Yuzaomian-9110 during 2017 and 2018, respectively. The accumulation of reproductive organ biomass was 21.0%–52.1% and 28.5%–56.8% higher in Lu-54 and 24.2%–56.8% and 34.8%–69.1% higher in Yuzaomian-9110 in P1 and P2 over the control, respectively. During the fast accumulation period, the average accumulation of N, P, K, and biomass across the years in P2 were recorded as 0.75, 0.6, 0.5, and 120.5 kg ha−1 d−1 in Lu-54, while they were 0.65, 0.5, 0.8, and 98.5 kg ha−1 d−1 in Yuzaomian-9110. Overall, a longer period, in terms of reproductive biomass accumulation, was recorded for Yuzaomian-9110 compared with Lu-54 in 2017 and vice versa across the 2018 growing season. The results suggested that increasing P rate improved yield, reproductive organ biomass, as well as nutrient accumulation in both cotton cultivars. However, low P-sensitive cultivar (Lu-54) was more responsive to P application compared with low P-tolerant cultivar.

Effects of increased precipitation on growth of two ephemeral plants in the Gurbantunggut Desert, China

Ephemeral plants are a major component of biodiversity in the deserts of Northwest China, with important ecological functions. Information on plant morphological characteristics and biomass accumulation and allocation during different growth stages could enhance our understanding of the functional features and survival strategy of ephemeral plants. We examined the effects of increased precipitation on ephemeral species Eremopyrum distans and Nepeta micrantha in the Gurbantunggut Desert. Changes in morphological characteristics and biomass accumulation and allocation were analyzed under increasing precipitation 30% and 50% treatments. The results showed that increased precipitation 30% and 50% treatments promoted the growth of E. distans, with leaf area and reproductive organ biomass being increased by 14.2%-188.5% and 55.9%, respectively. The effects of increased precipitation on the growth of N. micrantha varied across different growth stages. At leaf expansion stage, increased precipitation promoted growth of N. micrantha, while at fruit ripening stage, increased precipitation 50% treatment reduced leaf area, plant height, and reproduction biomass by 54.9%, 20.5%, 43.2%, respectively. Thus, the responses of the two species to increased precipitation was species-specific. Increased precipitation would change the survival strategies of the two species, with consequence on the species composition and structure of desert community.

Plant Density Influences Reproductive Growth, Lint Yield and Boll Spatial Distribution of Cotton

The number of cotton plants grown per unit of ground area has gained attention due to the high prices of inputs and lower production. Cotton yield per unit of area in Henan province has been stagnant in the last few years. The objectives of this study were to investigate cotton growth, yield, boll spatial distribution and biomass accumulation using different plant densities at cultivation and to find out the optimal plant density. A 2-year field experiment was conducted in a randomized complete block design under six plant densities (D1, 15,000; D2, 33,000; D3, 51,000; D4, 69,000; D5, 87,000 and D6, 105,000 ha−1). Cotton grown at lower plant density produced taller plants and high number of leaves per plant while greater number of branches, fruiting nodes and high number of bolls per unit of ground area were produced under high plant density. Boll retention rate decreased as plant population increased and at nodes 1–8 the rate decreased slowly and then increased dramatically. The highest seed cotton yield (4546 kg ha−1) and lint yield (1682 kg ha−1) was produced by D5. The seed cotton and lint yield produced by D5 were 51–55%, 40–37%, 22–26%, 11–15%, 12–15%, 28–30%, 21–24%, 15–20%, 7–13% and 13–17% higher than D1, D2, D3, D4 and D6 during both years of experimentation, respectively. The increase in seed yield was due to higher biomass accumulation in reproductive organs under D5 plant density. The highest average (110.4 VA kg ha−1 d−1) and maximum (126 VM kg ha−1 d−1) rate of reproductive organs biomass was also accumulated by D5 as compared to other plant densities. The results suggest that D5 is the optimal plant density for high reproductive biomass accumulation and high yield for the area of Henan province.

Moderate Drip Irrigation Level with Low Mepiquat Chloride Application Increases Cotton Lint Yield by Improving Leaf Photosynthetic Rate and Reproductive Organ Biomass Accumulation in Arid Region

Due to the changing climate, frequent episodes of drought have threatened cotton lint yield by offsetting their physiological and biochemical functioning. An efficient use of irrigation water can help to produce more crops per drop in cotton production systems. We assume that an optimal drip irrigation with low mepiquat chloride application could increase water productivity (WP) and maintain lint yields by enhancing leaf functional characteristics. A 2-year field experiment determines the response of irrigation regimes (600 (W1), 540 (W2), 480 (W3), 420 (W4) 360 (W5) m3 ha−1) on cotton growth, photosynthesis, fiber quality, biomass accumulation and yield. Mepiquat chloride was sprayed in different concentration at various growth phases (see material section). Result showed that W1 increased leaf area index (LAI) by 5.3–36.0%, net photosynthetic rate (Pn) by 3.4–23.2%, chlorophyll content (Chl) by 1.3–12.0% than other treatments. Improvements in these attributes led to higher lint yield. However, no differences were observed between W1 and W2 in terms of lint and seed cotton yield, but W2 increased WP by 3.7% in both years. Compared with other counterparts, W2 had the largest LAI (4.3–32.1%) at the full boll stage and prolonged reproductive organ biomass (ROB) accumulation by 30–35 d during the fast accumulation period (FAP). LAI, the average (VT) and maximum (VM) biomass accumulation rates of ROB were positively correlated with lint yield. In conclusion, the drip irrigation level of 540–600 m3 ha−1 with reduced MC application is a good strategy to achieve higher WP and lint yield by improving leaf photosynthetic traits and more reproductive organ biomass accumulation.

Pre-Sowing Irrigation Plus Surface Fertilization Improves Morpho-Physiological Traits and Sustaining Water-Nitrogen Productivity of Cotton

The changing climatic conditions are causing erratic rains and frequent episodes of moisture stress; these impose a great challenge to cotton productivity by negatively affecting plant physiological, biochemical and molecular processes. This situation requires an efficient management of water-nutrient to achieve optimal crop production. Wise use of water-nutrient in cotton production and improved water use-efficiency may help to produce more crop per drop. We hypothesized that the application of nitrogen into deep soil layers can improve water-nitrogen productivity by promoting root growth and functional attributes of cotton crop. To test this hypothesis, a two-year pot experiment under field conditions was conducted to explore the effects of two irrigation levels (i.e., pre-sowing irrigation (W80) and no pre-sowing irrigation (W0)) combined with different fertilization methods (i.e., surface application (F10) and deep application (F30)) on soil water content, soil available nitrogen, roots morpho-physiological attributes, dry mass and water-nitrogen productivity of cotton. W80 treatment increased root length by 3.1%–17.5% in the 0–40 cm soil layer compared with W0. W80 had 11.3%–52.9% higher root nitrate reductase activity in the 10–30 cm soil layer and 18.8%–67.9% in the 60–80 cm soil layer compared with W0. The W80F10 resulted in 4.3%–44.1% greater root nitrate reductase activity compared with other treatments in the 0–30 cm soil layer at 54–84 days after emergence. Water-nitrogen productivity was positively associated with dry mass, water consumption, root length and root nitrate reductase activity. Our data highlighted that pre-sowing irrigation coupled with basal surface fertilization is a promising option in terms of improved cotton root growth. Functioning in the surface soil profile led to a higher reproductive organ biomass production and water-nitrogen productivity.

Cadmium accumulation, translocation, and assessment of eighteen Linum usitatissimum L. cultivars growing in heavy metal contaminated soil

Flax (Linum usitatissimum L.) is suitable for growing in heavy metal polluted soil for non-food purposes and can be used as potential crops for cleaning the soil from heavy metals. The main objective of this study was to investigate flax/linseed phytoextraction of cadmium (Cd), including uptake, translocation, and accumulation differences in organs among flax/linseed cultivars. A field experiment was carried out in Cd contaminated field of Chinese southern area with nine flax, one dual-purpose, and eight linseed cultivars. Cd concentrations in plant organs (root, xylem, phloem, and capsule) and biomass of different organs of each cultivar were measured and evaluated. Significant differences in Cd concentration and accumulation among organs were observed. The most Cd was accumulated by phloem, which was 2 ∼ 4 times more than other organs, followed by roots, xylems and capsules played a comparably smaller role. The uptake of Cd by flax/linseed from per hectare was calculated and had highly significant positive correlation with biomass. Phloem accumulated 45 ∼ 55% of total Cd from the soil by the plant. Among testing 18 flax/linseed cultivars, one flax cultivar (Zhongya 1), and two linseed cultivars (Y2I329 and Y2I330), which extracted more than 60 g ha−1, can be considered as Cd accumulators for phytoremediation of Cd contaminated soil.

Soil Temperature Prior to Veraison Alters Grapevine Carbon Partitioning, Xylem Sap Hormones, and Fruit Set

To gain a better understanding of environmental effects on grapevines and the physiological regulation of acclimation, we determined the effects of soil temperature (14 or 24°C) between anthesis and veraison on growth, nonstructural carbohydrates, cytokinins, abscisic acid, and leaf function of potted Vitis vinifera cv. Shiraz. Plants of each regime were selected from two groups that had been grown in a glasshouse from three weeks prior to budbreak at an average soil temperature of either 13 or 23°C. Soil temperature between anthesis and veraison affected utilization and restoration of root and trunk nonstructural carbohydrates and changes in biomass of major plant organs. Soil warming promoted shoot growth via utilization of starch reserves, while soil cooling promoted starch storage in both the root and wood and shifted overall biomass partitioning to the roots. A change in soil temperature from warm to cool through flowering was also associated with reduced fruit set. Diurnal courses of photosynthesis, transpiration, and stomatal conductance after fruit set were significantly affected by soil temperature. Phytohormones (cytokinin and abscisic acid) were measured in the xylem sap and leaves at fruit set and veraison. Differences between these two sample types during grapevine development highlight a phytohormone shift likely involved in postveraison fruit ripening. We conclude that soil temperature significantly affects grapevine growth and that the responses are mediated largely by an influence of temperature on mobilization of nonstructural carbohydrates from the roots.

Gas exchange, biomass and non-structural carbohydrates dynamics in vines under combined drought and biotic stress

BackgroundIntensity of drought stress and pest attacks is forecasted to increase in the near future posing a serious threat to natural and agricultural ecosystems. Knowledge on potential effects of a combined abiotic-biotic stress on whole-plant physiology is lacking. We monitored the water status and carbon metabolism of a vine rootstock with or without scion subjected to water shortening and/or infestation with the sucking insect phylloxera (Daktulosphaira vitifoliae Fitch). We measured non-structural carbohydrates and biomass of different plant organs to assess the stress-induced responses at the root, stem, and leaf level. Effects of watering on root infestation were also addressed.ResultsHigher root infestation was observed in drought-stressed plants compared to well-watered. The drought had a significant impact on most of the measured functional traits. Phylloxera further influenced vines water and carbon metabolism and enforced the sink strength of the roots by stimulating photosynthates translocation. The insect induced carbon depletion, reprogramed vine development, while preventing biomass compensation. A synergic effect of biotic-abiotic stress could be detected in several physiological and morphological traits.ConclusionsOur results indicate that events of water shortage favour insects’ feeding damage and increase the abundance of root nodosities. Root phylloxera infestation imposes a considerable stress to the plants which might exacerbate the negative effects of drought.

Efficient improvement of soil salinization through phytoremediation induced by chemical remediation in extreme arid land northwest China

This study investigated the influence of chemical remediation agents (Bc, M, HA, and Bc + HA) on the growth of the halophyte Lycium ruthenicum and the mechanism of restoration of soil salinization using joint halophyte and chemical remediation in arid fields. The results showed that aboveground organ biomass of L. ruthenicum increased significantly with the chemical remediation agents analyze but the effects on the root system were different. Among the root traits, dry weight of the taproot of L. ruthenicum increased significantly (p < 0.05) by 60.57% with HA; however, the lateral roots were inhibited. With the addition of biochar, the content of sodium ions in roots increased significantly. Further analysis showed that endogenous manganese (Mn) promoted K+ absorption concentration increase from 22.09 to 38.28 g/kg. Moreover, Joint L. ruthenicum and chemical remediation with Bc, HA, M and Bc + HA reduced Na+ to 5854.76, 9396.19, 6530.95 and 11164.29 g/(kg DW⋅m2·a), respectively. Tests revealed that for L. ruthenicum, the aboveground biomass and root morphological plasticity, as well as the synergistic effect of K+ on Na+ transport capacity influenced by endogenous Mn in leaves, were the primary causes of the efficient improvement of saline-alkali land.

Plasticity in Sexual Dimorphism Enhances Adaptation of Dioecious Vallisneria natans Plants to Water Depth Change.

Sexual dimorphism in vegetative and reproductive traits is associated with contrasting strategies of males and females for response to varied environmental conditions, causing sex-specific reproduction success and consequently long-distance dispersal and colonization. Aquatic plants usually exhibit rich phenotypic plasticity and great diversity in reproductive systems, but the influence of aquatic conditions on the plasticity of sexual dimorphism has received less attention. Using a common garden experiment with dioecious submerged plant Vallisneria natans grown at various water depths simulating different light availability, we measured variations in 20 traits for females and 19 traits for males (total = 540 plants from 30 seed families) including morphology, reproductive traits and photosynthesis. We investigated sex-specific plastic responses and variation of sexual dimorphism in response to water depth change. Females displayed much greater leaf length, vegetative biomass and resource allocation to reproduction than males at all depths, whereas spathe number and gamete production per spathe displayed reverse pattern. Besides most traits in each sex (16 in female and 12 in male) showing striking phenotypic plasticity, the degree of sexual dimorphism increased significantly for total biomass and reproductive investment, but decreased for leaf length, spathe number and flowering ramet percentage in low light and deep water. Females varied more than males in leaf length, total biomass, reproductive investment, length and biomass of reproductive organs and rate of photosynthesis in response to decreased underwater light availability, suggesting that female has greater plasticity than male. These findings illustrated considerable plasticity in the degree of sexual dimorphism in a variety of vegetative and reproductive traits across different environments driven by the contrasting reproductive functions of the sexes in relation to pollen and seed dispersal. Females of V. natans responded more plastically than males to low light conditions resulted from water depth variation in either aboveground vegetative growth or reproduction. This study provides novel insight into adaptive strategies of submerged dioecious macrophytes to survive and increase fitness in freshwater habitats.

Effects of different rootstocks on cadmium accumulation of grafted Cyphomandra betacea seedlings

ABSTRACT To study whether grafting can reduce the heavy metal uptake of Cyphomandra betacea, C. betacea seedlings were grafted onto three different rootstocks (eggplant, Solanum nigrum, and tomato) and planted in cadmium (Cd)contaminated soil. Compared with ungrafted seedlings, S. nigrum rootstock increased the biomass of different organs of C. betacea seedlings, while eggplant and tomato rootstocks decreased it or had no significant effect. Chlorophyll a, chlorophyll b, and total chlorophyll contents of C. betacea seedlings were ranked as follows: S. nigrum rootstock > ungrafted > eggplant rootstock > tomato rootstock. The three rootstocks had no significant effects on the superoxide dismutase activity of C. betacea seedlings. Solanum nigrum rootstock enhanced the peroxidase and catalase activities and soluble protein content of C. betacea seedlings, while the other rootstocks reduced or had no significant influence on these parameters. The three rootstocks increased the Cd content of aboveground organs of C. betacea seedlings as follows: S. nigrum rootstock > eggplant rootstock > tomato rootstock > ungrafted. Grafting onto S. nigrum rootstock can therefore promote the growth of C. betacea; however, all rootstocks investigated in this study promote Cd uptake in C. betacea and thus cannot be used in Cd-contaminated areas.