Beetle Research Articles

Development of immature stages of Heteronychus arator rugifrons (Fairmaire, 1871) (Coleoptera: Dynastidae), a major crop pest in Madagascar

Adults (black beetles), and to a lesser extent, larvae (white grubs), of Heteronychus arator rugifrons (Fairmaire) are major crop pests of cereals—particularly upland rice—in the central highlands of the island of Madagascar. Since the biology of this endemic subspecies is far less well understood than that of the African black beetle Heteronychus arator F., we undertook laboratory studies to determine the developmental durations of its immature stages at two temperatures, with measurements taken (body length and weight of all stages/instars, head capsule width of all larval instars). The mean immature stage development period was significantly shorter at 25°C (160.8 ± 11.60 d) than at 18°C (251.4 ± 11.93 d), mainly due to shorter egg incubation, L1 instar and pupal periods. Some morphological traits of immature stages confirmed that H. arator rugifrons is a distinct subspecies from H. arator. In particular, head capsule width was homogenous among larval instars, irrespective of the rearing temperature, with no overlap across instars. Head capsule width range was 1.04–1.52 for L1, 1.72–2.36 for L2, and 2.40–3.48 for L3. This information should be helpful in studies of population dynamics and economic threshold calculations with a view to management of this pest.

Ophiocordyceps salganeicola, a parasite of social cockroaches in Japan and insights into the evolution of other closely-related Blattodea-associated lineages

The entomopathogenic genus Ophiocordyceps includes a highly diverse group of fungal species, predominantly parasitizing insects in the orders Coleoptera, Hemiptera, Hymenoptera and Lepidoptera. However, other insect orders are also parasitized by these fungi, for example the Blattodea (termites and cockroaches). Despite their ubiquity in nearly all environments insects occur, blattodeans are rarely found infected by filamentous fungi and thus, their ecology and evolutionary history remain obscure. In this study, we propose a new species of Ophiocordyceps infecting the social cockroaches Salganea esakii and S. taiwanensis, based on 16 years of collections and field observations in Japan, especially in the Ryukyu Archipelago. We found a high degree of genetic similarity between specimens from different islands, infecting these two Salganea species and that this relationship is ancient, likely not originating from a recent host jump. Furthermore, we found that Ophiocordyceps lineages infecting cockroaches evolved around the same time, at least twice, one from beetles and the other from termites. We have also investigated the evolutionary relationships between Ophiocordyceps and termites and present the phylogenetic placement of O. cf. blattae. Our analyses also show that O. sinensis could have originated from an ancestor infecting termite, instead of beetle larvae as previously proposed.

Diversidade de mesoinvertebrados associada ao tratamento aeróbio de resíduos sólidos orgânicos domiciliares

Os mesoinvertebrados provocam mudanças nas propriedades físicas e químicas da matéria orgânica durante o processo de compostagem. São excelentes recicladores de matéria orgânica. O objetivo deste estudo foi avaliar a densidade e a diversidade de mesoinvertebrados em sistema de tratamento aeróbio de resíduos sólidos orgânicos domiciliares. O trabalho realizado em Campina Grande, estado da Paraíba, Brasil, seguiu os princípios da pesquisa experimental. Foram coletados resíduos sólidos orgânicos em 46 residências, previamente segregados na fonte geradora. Por 15 semanas o sistema foi monitorado diariamente. Durante a compostagem foram coletados 1.258 indivíduos pertencentes à seis ordens: Diptera (88,5%), Mesostigmata (5,5%), Coleoptera (2,4%), Isopoda (2,4%), Araneae (0,6%) e Orthoptera (0,6%). No processo de compostagem foram observadas três fases distintas, nas quais as espécies foram diferenciadas. Constatou-se que no sistema de tratamento dos resíduos sólidos orgânicos domiciliares, há considerável participação de organismos que atuam nas diferentes fases do tratamento, correlacionando-se com as mudanças físico-químicas e influenciando no funcionamento de todo o sistema.

Biology and parasites of Pteronotus gymnonotus from the Caatinga shrublands of Ceará (Brazil)

Mormoopid bats are distributed from southern United States of America to Brazil and comprise the genera Mormoops and Pteronotus. Although forms of Mormoops in Bahia, Brazil were described for the Quaternary, only some of the extant species of Pteronotus occur in this country, including P. gymnonotus. The species distribution ranges from southeastern México to northeastern Bolivia and central Brazil. This work presents information about food preference, reproduction, and the ectoparasitological fauna of P. gymnonotus in the state of Ceará. Fieldwork took place over ten consecutive days in the rain and dry seasons, in 2000, 2012, 2013, and 2019, on trails within the Serra das Almas Private Natural Heritage Reserve. A total of 14 P. gymnonotus specimens were caught in the three main phytophysiognomies present in the region. They were inspected for ecto and endoparasites, and fecal material was collected and analyzed under a light microscope. Of the total collected females, four were pregnant and one was lactating in January, during the rain season. No food residues or evidence of endoparasites were observed in the gastric content of the animals. Regarding the fecal samples, fragments of insects were found and identified as belonging to the orders Coleoptera and Lepidoptera. Seven individuals were infested with ectoparasites of the subfamily Acari and newly described associations with Diptera genera are presented. Due to the scarcity of data about these bats in the Caatinga biome, data reported here help explain the ecological interactions of P. gymnonotus in this semiarid area in Brazil.

Female calling, life cycle, and microstructures of the parasitic beetle Ripidius quadriceps Abeille de Perrin.

The precopulatory behaviour of the larviform females of Ripidius quadriceps Abeille de Perrin, 1872 is described. The calling posture of virgin females is documented. The cephalic morphology and microstructures are visualised using scanning electron microscopy, in particular the secretory pores in the cuticle of inflatable maxillary palps. An exhaustive overview of relevant publications revealed that the location of secretory pores on the head of females is unique within the order Coleoptera. Compared to other beetles with sedentary calling females, the calling phase of the short-lived and non-feeding female of Ripidius is exceptionally short. For bioassays, various traps using virgin females of Ripidius were tested. It is likely that the sedentary behaviour of the short-lived female combined with a unique morphology and priority for investing in reproduction is compensated for by the actively flying males with remarkably flabellate antennae. The life cycle of this species, including some of the exceptions recorded at the individual level, is discussed. Perspectives for a biological and morphological survey of this rarely collected western Palaearctic species are outlined. In addition, the calling behaviour, secretory sites and location of pheromone glands in females of Coleoptera producing long range pheromones is reviewed.

New host and distribution records for Buprestidae (Coleoptera) from the state of Morelos, Mexico.

New state and host records are provided for 28 species of Buprestidae collected in Morelos, Mexico in the following genera: Acmaeodera Eschscholtz 1829, Actenodes Dejean 1833, Agrilus Curtis 1825, Brachys Dejean 1833, Chrysobothris Eschscholtz 1829, Leiopleura Deyrolle 1864, Lius Deyrolle 1864, Pachyschelus Solier 1833, and Tetragonoschema Thomson 1857.

A translation enhancer element from black beetle virus engages yeast eIF4G1 to drive cap-independent translation initiation

Cap-independent translation initiation plays crucial roles in fine-tuning gene expression under global translation shutdown conditions. Translation of uncapped or de-capped transcripts can be stimulated by Cap-independent translation enhancer (CITE) elements, but the mechanisms of CITE-mediated translation initiation remain understudied. Here, we characterized a short 5ʹ-UTR RNA sequence from black beetle virus, BBV-seq. Mutational analysis indicates that the entire BBV-seq is required for efficient translation initiation, but this sequence does not operate as an IRES-type module. In yeast cell-free translation extracts, BBV-seq promoted efficient initiation on cap-free mRNA using a scanning mechanism. Moreover, BBV-seq can increase translation efficiency resulting from conventional cap-dependent translation initiation. Using genetic approaches, we found that BBV-seq exploits RNA-binding properties of eIF4G1 to promote initiation. Thus, BBV-seq constitutes a previously uncharacterized short, linear CITE that influences eIF4G1 to initiate 5′ end-dependent, cap-independent translation. These findings bring new insights into CITE-mediated translational control of gene expression.

Freshwater insects CONUS: A database of freshwater insect occurrences and traits for the contiguous United States.

MotivationFreshwater insects comprise 60% of freshwater animal diversity; they are widely used to assess water quality, and they provide prey for numerous freshwater and terrestrial taxa. Our knowledge of the distribution of freshwater insect diversity in the USA is incomplete because we lack comprehensive, standardized data on their distributions and functional traits at the scale of the contiguous United States (CONUS). We fill this knowledge gap by presenting Freshwater insects CONUS: A database of freshwater insect occurrences and traits for the contiguous United States. This database includes 2.05 million occurrence records for 932 genera in the major freshwater insect orders, at 51,044 stream locations sampled between 2001 and 2018 by federal and state biological monitoring programmes. Compared with existing open‐access databases, we tripled the number of occurrence records and locations and added records for 118 genera. We also present life‐history, dispersal, morphological and ecological traits and trait affinities (analogous to fuzzy‐coded traits) for 1,007 stream insect genera, assembled from existing databases, reference books and the primary literature. We nearly doubled the number of traits for 11 trait groups and added traits for 180 genera that were not available from open‐access databases. Our database, Freshwater insects CONUS, facilitates the mapping of freshwater insect taxonomic and functional diversity and, when paired with environmental data, will provide a powerful resource for quantifying how the environment shapes stream insect diversity and taxon‐specific distributions.Main types of variables containedGeoreferenced occurrence records and traits for stream insects.Spatial location and grainContiguous United States at a grain of c. 1 m2.Time period and grainOccurrence records from January 2001 to December 2018, with 1‐day temporal resolution. Traits from January 1911 to December 2018.Major taxa and level of measurementGenera from the orders Coleoptera, Diptera, Ephemeroptera, Hemiptera, Lepidoptera, Megaloptera, Neuroptera, Odonata, Plecoptera and Trichoptera.Software format.csv.

Annotated Catalogue of the Superfamily Dascilloidea (Coleoptera: Elateriformia) of Iran

The fauna of Iranian Dascillidae and Rhipiceridae (Coleoptera: Dascilloidea) is summarized in this paper. In total, 6 species of Dascillidae from 2 genera, Dascillus Latreille, 1797 (1 species) and KarumiaEscalera, 1913 (5 species), and 2 species of Rhipiceridae from the genus Arrhaphipterus Schaum, 1862 are listed.The lectotype of K. estaphilinoidesEscalera, 1913 is to be considered the holotype.

Palaeoentomological study of the lacustrine oil-shales of the lower Miocene San Chils locality (Ribesalbes-Alcora Basin, Castellón province, Spain)

For the first time, the study of the fossil record present in laminated bituminous dolostones from the San Chils locality, lower Miocene in age (ca. 19 Ma), located at the Ribesalbes-Alcora Basin, is addressed. The identification of 11 families and three genera belonging to six insect orders (Orthoptera, Thysanoptera, Hemiptera, Coleoptera, Hymenoptera and Diptera) has been carried out. Furthermore, ephippia of ladoceran crustaceans, one bird feather, vertebrate coprolites and plant remains have been found. The San Chils palaeodiversity has been compared to that of La Rinconada, a similar outcrop in the same basin and depositional unit. Both localities correspond to lacustrine deposits and were mined during the past two centuries. This work has increased the palaeontological richness of the Ribesalbes-Alcora Basin with new fi ndings such as specimens of the subfamily Corixinae (Hemiptera: Corixidae), some ant workers (Hymenoptera: ormicidae), a specimen assigned to the genus Exechia (Diptera: Mycetophilidae), ephippia of Daphnia (Ctenodapnia ) sp. (Branchiopoda: Cladocera), the possible first report of the genus Sciocoris (Hemiptera: Pentatomidae) for the Neogene fossil record and the possible presence of the family Aeolothripidae (Thysanoptera). The current collection of fossil specimens from San Chils is small, but shows that it shared similar palaeoenvironmental conditions to that of La Rinconada, both in the lake waters and in the surroundings.

Genetic Manipulation of the Ergot Alkaloid Pathway in Epichloë festucae var. lolii and Its Effect on Black Beetle Feeding Deterrence.

Epichloë endophytes are filamentous fungi (family Clavicipitaceae) that live in symbiotic associations with grasses in the sub family Poöideae. In New Zealand, E. festucae var. lolii confers significant resistance to perennial ryegrass (Lolium perenne) against insect and animal herbivory and is an essential component of pastoral agriculture, where ryegrass is a major forage species. The fungus produces in planta a range of bioactive secondary metabolites, including ergovaline, which has demonstrated bioactivity against the important pasture pest black beetle, but can also cause mammalian toxicosis. We genetically modified E. festucae var. lolii strain AR5 to eliminate key enzymatic steps in the ergovaline pathway to determine if intermediate ergot alkaloid compounds can still provide insecticidal benefits in the absence of the toxic end product ergovaline. Four genes (dmaW, easG, cloA, and lpsB) spanning the pathway were deleted and each deletion mutant was inoculated into five different plant genotypes of perennial ryegrass, which were later harvested for a full chemical analysis of the ergot alkaloid compounds produced. These associations were also used in a black beetle feeding deterrence study. Deterrence was seen with just chanoclavine present, but was cumulative as more intermediate compounds in the pathway were made available. Ergovaline was not detected in any of the deletion associations, indicating that bioactivity towards black beetle can be obtained in the absence of this mammalian toxin.

Variation of soil arthropods abundance and diversity between two sites of the Punjab province in Pakistan

Soil Arthropods are a vitally important component of the ecosystem, as their diversity, abundance, and distribution pattern play a vital role in ecosystem sustainability. Their population is expected to vary according to geographical patterns and environmental variables such as temperature, and humidity. For this purpose, we collected soil arthropods from the two regions having different latitudinal patterns: FSD (Faisalabad) and MGRH (Muzaffargarh). The soil faunal samples were collected fortnightly from the mango orchards for two consecutive years, 2017 and 2018. During the whole research, a total of 8301 specimens of (16, 11) orders, (118, 57) species, and (5027, 3274) individuals were recorded from FSD and MGRH, respectively. Order Coleoptera found the maximum abundance in both regions among all orders. Our results showed that the FSD regions had a high abundance of arthropods compared to the MGRH region. In comparison, the different diversity factors and abundance revealed a strong correlation with the abiotic factors. They showed a high pattern of distribution and richness at the FSD region, as low temperature and high humidity were observed in the FSD region that influences the more diversity occurrence of fauna. Our results predicted that the habitat type and environmental factors such as humidity and temperature play a significant role in the distribution pattern of arthropods in any geographical region.

Effect of substrates of native and exotic plant species on the initial period of colonization of benthic macroinvertebrates in the Cerrado biome

The introduction of exotic species can generate changes in the composition of organic debris of alien origin in aquatic ecosystems close to the places of introduction. This new debris can cause impacts at the level of communities and ecosystems. Therefore, the present work aims to answer the following question: are the macroinvertebrate communities present in substrates formed by leaves of the exotic species Pinus caribaea Morelet (Pinaceae) and Eucalyptus grandis Hill ex Maiden (Myrtaceae) similar to those found in substrates composed by the mix of these two species and substrates composed by a mix of native species? For the collection of macroinvertebrates, sets of four types of artificial substrates were submerged in a stream inside the Ecological Station of Panga (Uberlândia—MG, Brazil). The treatments were: leaves of P. caribaea, leaves of E. grandis, leaves of both exotic species and leaves of two native species. In the 20 sample units, about 250 individuals were found, belonging to the orders Coleoptera, Diptera, Ephemeroptera, Heteroptera, Megaloptera, Odonata, Plecoptera and Trichoptera. The effects of the treatments were observed in the exponential decay of the substrate mass and in the structure of the benthic macroinvertebrates communities. The composition of these communities differed among treatments, with high dissimilarity observed between P. caribaea treatments and the mix of exotic species. Therefore, the results demonstrate the importance of substrate complexity for benthic macroinvertebrates, as well as the possible effects of biological invasion and co-invasion by species widely used in silviculture activities.

Short and Sweet: Viral 5`-UTR as a Canonical and Non-Canonical Translation Initiation Switch.

The replication of viruses requires host cell functions, specifically for protein synthesis, as viruses lack their own translational machinery. Failure to translate viral mRNAs and generate viral proteins would affect the propagation and evolution of a virus. Thus, independently of their size, complexity, and genomes, viruses evolved sophisticated molecular mechanisms to hijack the translational apparatus of a host in order to recruit ribosomes for efficient protein production. One of the prevalent mechanisms of translation regulation utilized by viruses is non-canonical translation initiation. It is often governed by the 5’-untranslated regions (5’-UTRs) present upstream of a protein-coding sequence in viral mRNAs, such as internal ribosome entry sites (IRESs) and capindependent translation enhancers (CITEs). Viruses can also utilize canonical translation initiation factors of a host in non-canonical ways. Understanding strategies and mechanisms used by viruses to generate proteins is an important task, as it might help develop new therapeutic interventions. We previously have demonstrated that mRNA from the genome of the black beetle virus (BBV) of the Nodaviridae family contains short and unstructured 5’-UTR, which governs translation initiation as a CITE and as a canonical translational enhancer. In this Commentary, we summarize cap-dependent and cap-independent translation initiation mechanisms and further elaborate on the unique ability of the BBV mRNA 5’-UTR to switch between these two modes of translation initiation in the context of the viral life cycle. Medical implications in treating the severe acute respiratory syndrome coronavirus 2 (SARS-CoV2) infection by targeting viral 5’-UTRs are also discussed.

Барсакелмес қорығы қаттықанаттыларының (Coleoptera) күзгі фаунасы

Coleoptera are the most diverse and species-rich order of insects. Biologically, coleoptera are very diverse. Beetle nutrition is also extremely diverse. Among them there are predators, phytophages, saprophages, necrophages. Predatory beetles prey on various small invertebrates, mainly other insects. Herbivorous beetles feed not only on the green parts of a wide variety of plants, but also on wood. Beetles are found everywhere (in water, on land, on plants, in soil) and are essential in the circulation of substances in nature. The research was carried out in October 2021 in the section of the Syrdarya River delta, up to the Koksarai dam, Amanatkol Lake, Shobankol Lake, Alakol Lake, Akshatau Lake, Shomishkol lake, okr. village. Shomishkol, Kolshykkan lake. Conventional entomological traditional methods were used in the conducted research. As a result of the field work, representatives from the Insect class (Insecta) of the order Coleoptera, or beetles (Coleoptera) were identified. A total of 24 species from 4 families are represented. The sem stands out for its species diversity. Tenebrionidae (12 species), Carabidae (3 species), in the other 2 families, only 1 species are noted. Studies in 2021 have shown a decrease in the number and faunal change of dominant species. Since the studies were conducted in October, many invertebrates have already gone to winter.

Coleoptera Fauna Beetles of Nest Birds of the Northern Caucasus

In this article, we present a faunistic list of species of the order Coleoptera from 13 families: 40 species collected from the nests of 24 bird species in the North Caucasus. To our previously published data, the list of coleopteran species has increased by 2 species. We were able to identify about 20 optional species that got into the bird's nest by accident. These species are not covered and discussed in this article. The rest of the species have complex biocenotic relationships with the host

Іле-Алатау МҰТП қаттықанаттылары (Coleoptera) (Оңтүстік-Шығыс Қазақстан)

The article presents the results of research conducted by the authors on the territory of the Ile-Alatau State National Natural Park in 2020-2021. Research works are devoted to the fauna, biology and ecology of beetles or beetles on the territory of this natural park. Coleoptera are the largest group of insects, which is of great practical importance. They are distributed on the territory of the IleAlatau GNPP. Most of the beetle species are zoophages, and phytophages also predominate. When collecting the material, standard entomological methods were used-collection with an entomological filter, collection with an artificial light source, manual collection, etc. As a result of the conducted research, 47 species from 10 families of the coleoptera order were collected on the territory of the Ile-Alatau State National Natural Park: Meloidae, Curculionidae, Buprestidae, Chrysomelidae (out of 8 species, 17%), Tenebrionidae, Cerambycidae, Scarabaeidae (of 3 species, 7%), Coccinellidae, Silphidae, Сarabidae (of 2 species, 4%). In this order, predatory beetles are nutritionally related: representatives of the family Coccinellidae, Carabidae, the remaining species from 8 families (Meloidae, Curculionidae, Buprestidae, Chrysomelidae, Tenebrionidae, Cerambycidae, Scarabaeidae, Silphidae) are phytophages. Coleoptera are divided into zoophages (4 species), phytophages (41 species), coprophages (1 species), saprophages (1 species) by nutritional connection. Coleoptera on the territory of the Ile-Alatau State National Natural Park differ into chortobionts (27), dendrobionts (17 species), herpetobionts (3 species). Beetles in the park are ecologically mesophilic species.

Bazı bitki ektraktlarının Sitophilus granarius (Linnaeus, 1758) (Coleoptera: Curculionidae) ve Tribolium castaneum (Herbst, 1797) (Coleoptera: Tenebrionidae) üzerindeki insektisidal ve davranışsal etkileri

Depolanmış ürünlerdeki böceklerle mücadele oldukça önemlidir. Sitophilus granarius (Linnaeus, 1758) (Coleoptera: Curculionidae) ve Tribolium castaneum (Herbst, 1797) (Coleoptera: Tenebrionidea) depolanmış ürünlerde zarar meydana getiren önemli iki zararlı böcek türüdür. Depolanmış ürünlerdeki zararlılarla mücadelede kullanılabilecek alternatif ilaçlara ise her geçen gün ihtiyaç artmaktadır. Bu çalışmada, Achillea phrygia Boiss. et Bal. (Compositae), Prangos ferulacea (L.) Lindl. (Apiaceae) ve Salvia wiedemannii Boiss. (Lamiaceae) bitkilerinden elde edilen bitki ekstraktlarının, S. granarius ve T. castaneum ile mücadelede kullanılabilirlikleri araştırılmıştır. 72 saat sonunda, S. garanarius üzerinde test edilen bitkiler arasında en yüksek kontak aktiviteyi sırasıyla %74 ile P. ferulaceae hekzan, %71 ile A. phrygia methanol ekstraktı göstermiştir. T. castaneum üzerinde en yüksek aktiviteyi ise 72 saat sonunda %42 ile A. phrygia etil asetat ekstraktı göstermiştir. Bitki ekstraktları ile yapılan davranışsal etki çalışmalarında ise S. granarius üzerindeki en yüksek repellent aktivite 24. saat sonunda P. ferulaceae etil asetat ekstraktından (%52) elde edilmiştir. Bitki ekstraktlarının tamamı T. castaneum üzerinde yüksek oranda repellent etki göstermiştir.

Sexual Dimorphism and Feeding Ecology of the Black-bellied Bunchgrass Lizard Sceloporus aeneus (Squamata: Phrynosomatidae) in Central Mexico

Morphology and resource use have a significant influence on lizard population dynamics. In this study, sexual dimorphism and feeding ecology were assessed in a population of Sceloporus aeneus from central Mexico. Sexual dimorphism was recorded, with males being larger than females in several morphological characteristics, such as jaw width, femur length, tibia length, and forearm length, but not snout–vent length (SVL) or jaw length, which were similar in both sexes. The stomach contents analysis indicated an insectivorous diet composed of 11 prey categories, of which females consumed 11 and males consumed 9. Low values of feeding niche breadth were found, as well as high overlap between sexes and seasons. For the whole population, adult preys of the family Formicidae and orders Coleoptera and Hemiptera showed the highest values of food importance and abundance. A higher volume of stomach contents was recorded during the dry season than the wet season in both females and males. Results showed a pattern of sexual dimorphism similar to that of other species of the genus Sceloporus, but different from those of other populations of the same species. Morphological variation in jaw length and width seems to be related to food resources use because, in spite of the high similarity in the diet between sexes, the volume of stomach contents was not correlated with SVL in females. These findings suggest a constant consumption of food that is subsequently invested in reproduction. Further studies analyzing morphology and diet and their effect on reproductive characteristics such as clutch size, eggs mass, and volume are needed among populations of this species. The results obtained in this research could help explain the differences and similarities previously reported on the morphology and reproductive characteristics for S. aeneus and other species of the S. scalaris group.

KEANEKARAGAMAN ARTHROPODA PADA PERTANAMAN BAWANG MERAH DENGAN INTENSITAS APLIKASI PESTISIDA YANG BERBEDA DI KABUPATEN SIGI

Biodiversity is an important part of the agricultural ecosystem (agroecosystem) and diversity is determined by the environment and the application of pesticides in the field. Research on the diversity of arthropods in shallot cultivation with different intensities of pesticide application aims to determine the types and populations of arthropods in shallot cultivation on land with different intensities of pesticide application. The research was conducted in the village of Soulowe, which is the center of shallot plants in Sigi Regency, with a massive and intensive application of pesticides, from December 2019 to March 2020. Sampling was carried out using absolute methods (direct observation) and relative methods (Pitffal Traf and Jaring Ayun). The results of the identification carried out in the Unisa and Untad Palu laboratories found that the diversity of arthropods in low-intensity areas of pesticide application was more than that of medium and high applications. There are 7 orders and 13 families, namely Oxyopidae, Grillotalpidae (Ordo Araneae), Scarabaeidae, Coccinellidae (Order Coleoptera), Forficuloidea (Order Dermaptera), Agromyzidae, Muscidae (Order Diptera), Formicidae, Apidae (Order of Hymenoptera), Noctuidae (Order of Hymenoptera) and Acrididae, Gryllidae andTotigonidae (Order Orthoptera). The level of diversity, evenness and family wealth as well as the abundance of arthropod individuals were higher in land with low pesticide application intensity than medium and high pesticide application.