Orange Glow Research Articles

Polyphenolic Profiling and Antioxidant Activity in Berry Extracts of Pyracantha Wild Varieties from the Mediterranean Region.

Pyracantha is a genus of wild perennial shrubs native in an area extending from Southwest Europe to Southeast Asia, and it is used in traditional medicine for the diuretic, cardiac, and tonic properties of its fruits, which can also be cooked to make jellies, jams, and sauces. This work aims to study and compare the antioxidant activity and the phenolic and anthocyanin composition of three varieties of Pyracantha coccinea: Red Column (PCR), Orange Glow (PCO), and Soleil d'Or (PCS), and one of Pyracantha angustifolia: Orange glow (PAO), collected from the spontaneous flora of the Mediterranean region (Southern Italy). Two different extraction processes were tested using methanol and an aqueous methanol solution (80% MeOH) to evaluate the polyphenolic content and antioxidant activity of freeze-dried berries. The highest total phenolic content was found in PCR and PAO berries (174.21 ± 0.149 and 168.01 ± 0.691 mg of gallic acid equivalent per gram of dry matter, respectively) extracted with an aqueous methanol solution (80% MeOH). Polyphenolic extracts analyzed by HPLC-DAD-ESI/MS confirmed the presence of rutin, quercetin hexose, neoeriocitrin, procyanidin B, and resveratrol. Moreover, the total antioxidant activity of the berries' extracts was measured by comparing two different spectrophotometric methods (ABTS and DPPH), showing that the varieties with the highest total phenolic content, PCR and PAO, also had the highest scavenging activity. Finally, a suitable extraction process was chosen for the evaluation of the anthocyanins' composition of all frozen berries, and in all MS spectra of Pyracantha varieties, two ionic species at 449 m/z attributable to two cyanidin derivatives were found.

Blue pill or red pill? Green, thanks. Behavioural test on coloured light preference in the Calabrian Alpine newt to increase the capture rate

Abstract To improve monitoring techniques, we investigated the colour vision of the Calabrian Alpine newt (Ichthyosaura alpestris inexpectata). We conducted controlled behavioural experiments on male and female newts to explore their response to artificial coloured light sources in the dark. We used green, blue, and orange glow sticks. Observations indicated that both sexes strongly preferred green glow sticks, which were selected in almost 50% of the trials Additionally, newts exhibited markedly swifter reactions to green light. The attraction to green glow sticks may be due to the biofluorescence within the green wavelength spectrum in this newt. This glowing ability, which resembles the green glow stick’s glow, could be beneficial in low-light conditions to locate other newts. Our results show that the behavioural preferences towards a particular light source can significantly enhance the success of capturing newts during population monitoring and conservation efforts for this rare and threatened taxon.

The results of the introduction of Pyracantha М. Roem. representatives in the conditions of the National Dendrological Park “Sofiyivka” of the NAS of Ukraine

Based on the analysis of long-term data, it was revealed that the weather and climatic conditions of the Right-Bank forest-steppe of Ukraine are pretty favourable for the course of seasonal rhythms of plant development studied species. P. coccinea (total points 83), P. crenatoserrata (total points 76), and P. crenulata (total score 81) are pretty promising species according to the scale for assessing the prospects of introduction of woody plants. Such species as P. × ‘Orange Charmer’ (sum of points 83) is quite promising among the varieties, and P. × ‘Red Column’ (sum of points 61), and P. × ‘Orange Glow’ (sum of points 66) are less promising. The least promising plant is P. × ‘Red Cushion’ (sum of points 42), and P. × ‘Soleil d’Оr’ (sum of points 37) is unpromising.

The University of Texas's “Racial Geography Tour,” designed by Dr. Edmund Gordon

The University of Texas's “Racial Geography Tour,” designed by Dr. Edmund Gordon

Biodiversity and Habitats of Polar Region Polyhydroxyalkanoic Acid-Producing Bacteria: Bioprospection by Popular Screening Methods.

Polyhydroxyalkanoates (PHAs), the intracellular polymers produced by various microorganisms as carbon and energy storage, are of great technological potential as biodegradable versions of common plastics. PHA-producing microbes are therefore in great demand and a plethora of different environments, especially extreme habitats, have been probed for the presence of PHA-accumulators. However, the polar region has been neglected in this regard, probably due to the low accessibility of the sampling material and unusual cultivation regime. Here, we present the results of a screening procedure involving 200 bacterial strains isolated from 25 habitats of both polar regions. Agar-based tests, microscopy, and genetic methods were conducted to elucidate the biodiversity and potential of polar-region PHA-accumulators. Microscopic observation of Nile Red stained cells proved to be the most reliable screening method as it allowed to confirm the characteristic bright orange glow of the Nile Red–PHA complex as well as the typical morphology of the PHA inclusions. Psychrophilic PHA-producers belonged mostly to the Comamonadaceae family (Betaproteobacteria) although actinobacterial PHA synthesizers of the families, Microbacteriaceae and Micrococcaceae also featured prominently. Glacial and postglacial habitats as well as developed polar region soils, were evaluated as promising for PHA-producer bioprospection. This study highlights the importance of psychrophiles as biodiverse and potent polyhydroxyalkanoate sources for scientific and application-aimed research.

Carotenoids, Flavonoids, Total Phenolic Compounds Content and Antioxidant Activity of Indigenous Pyracantha coccinea M. Roem. Fruits

Scarlet/red firethorn, Pyracantha coccinea M. Roem., is a common ornamental shrub, commonly cultivated nowadays in Romania and many other countries, in gardens or hedges. The tree has small white flowers and it produces small bright colored berries. The fruit is bitter and astringent, making it inedible when raw. The fruit can be cooked to make jellies, jams, sauces and marmalade. In this study, the Pyracantha coccinea M. Roem fruits from three common cultivars named �Orange Glow�, �Red Column� and �Soleil d�Or�, were collected from different individuals in Constanta city, Romania. Both frozen and dried (80�C) fruits were analyzed for the active principles content such as, carotenoids, flavonoids and total phenolic compounds correlated with antioxidant activity. Carotenoids were determined by acetone extraction and UV-Vis spectrophotometry, flavonoids, by methanol extraction and UV-Vis spectrophotometry and total phenolic, by methanol extraction, Folin-Ciocalteu reaction and UV-Vis spectrophotometry. The total antioxidant capacity was quantified through photochemiluminescence method by comparison with the standard substance used for calibration, Trolox� as tocopherol analogue (ACL procedure) using Photochem apparatus, Analytik Jena AG, Germany. The average carotenoid content in fresh frozen fruits ranged between 278-545 mg/kg (dry weight equivalent), while in dry material 133-203 mg/kg. Total flavonoids averaged 1415-4953 mg/kg in fresh fruits, increasing after heat-drying to 2981-5759 mg/kg. Fresh fruits contained a total amount of phenolic compounds of 11501-21107 mg/kg DW gallic acid equivalent, while dry fruits averaged 5749-8261 mg/kg GAE. Pyracantha coccinea M. Roem. fruits emphasized an increased antioxidant activity correlated with a high content of potentially bioactive compounds, especially of phenolic compounds, both in frozen and heat-dried state. �Red Column� and �Orange Glow� are the most promising varieties of the three cultivars, for potential pharmaceutical applications.

Isolation and identification of lipolytic bacteria from the digestive tract of eel (Anguilla bicolor bicolor)

Abstract. Hapsari RT, Susilowati A, Pangastuti A. 2020. Isolation and identification of lipolytic bacteria from the digestive tract of eel (Anguilla bicolor bicolor). Bonorowo Wetlands 10: 44-50. Anguilla bicolor bicolor is commonly found in the waters of western Indonesia. Currently, the demand for eel is increasing, causing an increase in the economic value of eel and being used as an export commodity. This study aims to isolate and identify lipolytic bacteria found in the digestive tract of eel (A. bicolor bicolor). Lipolytic bacteria isolated from the digestive tract of eel (A. bicolor bicolor). Bacterial isolation uses minimal media enriched with olive oil. When lipolytic bacteria were screened using Rhodamine B agar media, the presence of an orange glow in the colony upon exposure to 350 nm UV light indicated lipolytic activity. Identification of lipolytic bacteria was based on observing the morphological characters of bacterial colonies (edges, elevation, colonies, and colors) and the gene sequences encoding 16S rRNA. DNA sequence characteristics of lipolytic bacteria were analyzed by BLAST Nucleotide on the NCBI website (www.blast.ncbi.mlm.nih.gov/blast.cgi). Based on the screening results, 7 isolates of lipolytic bacteria were obtained from 30 successfully isolated isolates. The DNA sequence characteristics of the bacterial isolates were analyzed by BLASTN and found 5 species, namely Pseudomonas azotoformans, Providencia vermicola, Providencia sp., Aeromonas veronii, and Aeromonas hydrophila.

The Right Light: Tiger Salamander Capture Rates and Spectral Sensitivity

ABSTRACTKnowledge of population dynamics is critical for species of conservation concern so wildlife researchers need to take advantage of biases in animals' behaviors that could help them monitor populations. This is particularly important for amphibians because many populations are declining and difficult to observe. Attraction of vertebrates to light is a well‐known navigational cue and some fisheries strategies utilize light sources to increase catch success. To optimize population monitoring of state‐endangered eastern tiger salamanders (Ambystoma tigrinum), we tested the utility of colored glow sticks (green, orange, pink, or yellow) as bait in traps in Maryland and Delaware, USA, during 2015–2016. We then measured intensities of the colored glow sticks and estimated photoreceptive quantum catches of each color according to the spectral sensitivities of eastern tiger salamanders. We found that eastern tiger salamanders' photoreceptors had greater quantum catches of photons from green and yellow glow sticks. However, traps baited with orange glow sticks were more than twice as likely to capture eastern tiger salamanders than were traps baited with green or yellow. There were no differences in the number of salamanders caught per successful trap among glow stick colors, perhaps due to attraction of salamanders to motion from previously captured salamanders. Our results indicate that biases other than perceived intensity of light sources may drive behavioral preferences. © 2020 The Wildlife Society.

We Don't Need Another Hero: 10th Berlin Biennial for Contemporary Art

We Don't Need Another Hero: 10th Berlin Biennial for Contemporary Art

Spontaneous thermokinetic amplitude modulation in the “burning wire” lecture demonstration

A thin nichrome wire that is tensioned by a mass and driven by an alternating electrical current, with a magnet placed along the wire’s length, provides a popular lecture demonstration. Due to the convective cooling of the portions of the wire moving with the greatest velocity, in a darkened room, only the regions near the velocity nodes will be visible due to the orange glow of the heated wire. The thermal expansion coefficient of the wire forces the tensioning mass to change its height as temperature changes. By adiabatic invariance, the changes in the mass’s elevation cause the natural frequency to be shifted. Competition between the thermal inertia of the wire and the convective heat transfer coefficient introduces an exponential thermal relaxation time so that the amplitude of vibration is dependent on the ratio of the constant drive frequency to the changing resonance frequency at an earlier (retarded) time. These thermal and kinetic effects are incorporated into three coupled nonlinear ordinary differential equations that are separated by the method of multiple time scales and are solved numerically, reproducing both the spontaneous appearance of stable periodic amplitude modulation and the hysteretic behavior observed with the increasing or decreasing drive frequency.

The > friendly orange glow: The untold story of the PLATO system and the dawn of cyberculture

The > friendly orange glow: The untold story of the PLATO system and the dawn of cyberculture

As Fires Raged, Preparation, Teamwork Kept Disaster at Bay

As Fires Raged, Preparation, Teamwork Kept Disaster at Bay

Electrothermal Annealing of Catalytic Platinum Microwire Electrodes: Towards Membrane‐Free pH 7 Glucose Micro‐Fuel Cells

AbstractSurface activation and cleaning of platinum micro‐wires of 25 micron diameter has been achieved by a short one‐second electrothermal annealing treatment in air at 0.3 A (to orange glow). Voltammetric data suggest a decrease in the electrochemically active surface area with annealing time and a change in surface structure towards the Pt(100) crystalline face. The impact of electrothermal annealing on the electrocatalytic activity towards (i) oxygen reduction and (ii) glucose oxidation in pH 7 phosphate buffered aqueous solutions is investigated. In contrast to as‐received platinum, annealed platinum produces an electrocatalytic response towards glucose oxidation with increasing glucose concentration. A short one‐second anneal step is just as effective in enhancing catalysis as prolonged electrochemical cleaning in sulphuric acid. Finally, by combining a non‐annealed and an annealed microwire electrode, a very simple membrane‐free micro‐fuel cell system is devised operating in phosphate buffer at pH 7 with linearly increasing power output reaching 2.2 nW cm−2 for high glucose levels of 0.5 M.

Thorough Pictures of the Heart

You can hardly think what a pleasure it is to me to have such thorough pictures of your heart.-Jane Austen to Fanny Knight (20 February 1817)ONCE UPON A TIME A TOTAL STRANGER WROTE TO ME. I HAD HAD TWO OR THREE articles published in a Melbourne newspaper and one on the subject of migration appealed to her, for she was an englishwoman living in Australia. i was an Australian living in Greece. she did not have my address, nor did she expect the newspaper to forward her letter. instead, she used her powers of deduction, which proved to be considerable, made a shrewd guess at my location, and sent the pale blue aerogramme into the rather darker wide blue yonder. Hellenic post rose to the challenge as well, and so the letter eventually reached me.My correspondent in far-off Australia was surprised to hear that her letter had arrived, that the mission, so to speak, had been accomplished:Your letter arrived, which means my letter arrived. I can't believe it. As I pulled it out of the letterbox the wattle spilled pollen and blossom on to it, and I felt on the threshold of two worlds for a moment . . .I answered again, as was only polite; she replied, and the correspondence has now been going on for more than thirty years. this, then, was how Lesley and i came to meet. on paper-it would be five years before we met face-to-face.In this moment of writing i am also looking at two pictures propped on my desk, my eyes flicking between them and my computer screen. they were painted by artists who were interested, among other subjects, in the gentle art of correspondence, which was, at the time they were working, very much alive; these male artists liked to depict women either writing, receiving, or reading letters. the pictures i mention are separated in time by more than two centuries and were painted in very different places, but both are of women reading letters. the more recent one, the picture painted in Australia, is a 1908 work, The Letter, by e. phillips Fox. it shows a young, auburn-haired woman resting her right hand on a pink cheek; her left hand holds a letter that has been folded in the middle. she is standing in profile against a filmy curtain, and her dress is also filmy, with lace at its neck and on the ruffles edging the sleeve that has slipped down her right arm. Both dress and curtain are white, merging into grey; a darker grey seems to be the color of the flounces on the dress. the dress has a cream satin sash. the room is bathed in soft, pale light.Australia post, as it eventually became, had started almost a century before, in 1809, when ex-convict isaac nichols was given the job of boarding ships, collecting the mail, and then distributing it from his home in George street, sydney. thus the pandemonium of the general public rushing newly arrived ships was avoided. By 1908, the general public could expect two deliveries a day in big cities, while Mail day was a day to look forward to in country areas: the gigs and jinkers would be lined up waiting outside the General store.The other picture, Woman in Blue Reading a Letter, was painted by dutch master Johannes Vermeer during the period 1663-1664. dutch genre paintings of the seventeenth century were the first to depict anonymous people writing, reading, dispatching, and receiving letters. But correspondence then was a highly uncertain business: literate seamen who were on their way to and from the dutch east indies, for example, left their letters on a certain beach in Madagascar, where they knew or at least hoped that other ships would pick them up.Vermeer's woman, too, is standing in profile but is facing an unseen window and gripping her letter with both hands. there are two dark-blue chairs in the room; books lie on a desk. there is a box of pearls there, too, but they often go unnoticed. the woman is dressed in a richly heavy blue jacket and a brown-toned skirt. An orange glow suffuses this room, and a dark map hangs on the wall. …

Tracking the Development of Bipolar Disorder in Childhood

Tracking the Development of Bipolar Disorder in Childhood

Duck Tents (review)

Reviewed by: Duck Tents Hope Morrison Berry, Lynne . Duck Tents; illus. by Hiroe Nakata. Holt, 200926p. ISBN 978-0-8050-8696-6$16.95 R 4-7 yrs In this spirited verse tribute to the joys of camping, five little ducks (also starring in Duck Skates and Duck Dunks) pitch their tents in the backyard and embark on an evening of outdoor recreation. First they go fishing, albeit with limited success, then they roast marshmallows over a campfire ("Outside crispy, inside sticky,/ Chewy, gooey, finger-licky"), until finally it's time to tuck in for the night. Night-time noises keep the ducks awake, but when they collectively opt to ditch their solo tents and bunk up together, the problem is solved and they drift off to sleep. Berry's text is witty and playful, with descriptive details anchoring the simple narrative. The rhyming lines have a zesty cadence that nearly begs to be chanted aloud, and the lively verses pair perfectly with Nakata's ebullient watercolor illustrations. The art makes particularly good use of light and shadow, using them to indicate the movement of the sun through the day and casting an orange glow on the faces of the ducks as they sit fireside, and there is a warm domesticity in the friendship-focused scenes. This would be an excellent introduction to camping for the wary, as it describes many of the key elements while gently addressing the potentially scary parts. Copyright © 2009 The Board of Trustees of the University of Illinois

Meet a member: Eric Nyberg, releasing materials science into the wild

Hunkered down since before dawn on a colorful blanket of leaves covered by an early frost, Eric Nyberg prepares to draw back the string of his die-cast magnesium bow. Less than 25 feet from him stands a six-point bull elk. The orange glow of the sun rising above the mountains in eastern Washington illuminates the massive size of the elk and brings it into Nyberg’s focus. He waits for the perfect moment and releases the arrow—success. Nyberg, a senior research scientist at the Pacifi c Northwest National Laboratory in Richland, Washington, has been hunting for nearly 30 years in the state of Washington, and has taken deer and elk from several northwest states. His love for the nature-oriented sport has earned him two places in the Pope & Young Club Bowhunting Big Game Records of North America record book. “I began hunting as a teenager in Washington State,” he said. “In eastern Washington, hunting, fi shing, and other outdoor activities are very popular due to the open expanses, beautiful mountains, and abundant wildlife.” Prior to 2003, Nyberg did most of his hunting during rifl e season. It wasn’t until then that he was able to draw a “Non-Resident” elk hunting permit (“tag”) for the state of Montana and became interested in archery hunting. “Drawing the tag was going to be a hunting opportunity I would not miss, only problem was that it occurred during the rifl e hunting season in Washington State,” he said. “Therefore, I decided to take up archery hunting.” Nyberg said he practiced with his new bow all summer and within the fi rst month of becoming an archer he landed a six-point bull elk in Washington State. That same year he used the coveted “Montana tag” to take another six-point bull elk with a rifl e. Since then, he has hunted exclusively with a bow and arrow. “I love hunting because it is a chalMeet a Member: Eric Nyberg, Releasing Materials Science into the Wild

The perils of the light

I hate mornings. The delights of the rosy-fingered dawn are not for me unless I'm seeing it through a vague noisy haze, wondering if it is excessive to open another bottle of wine. Occasionally though, life and nature can produce a surprise. In deep December, on an overnight COOP shift, we set out in the dark on a late call to a quarry village on the edge of our area. Neither the driver or I knew it well. We became frustrated driving aimlessly around various unpromising-looking streets, lit in a drab orange glow by street lamps working late on a winter's morning, just like us. Suddenly, as we turned another corner the light changed dramatically. Out of range of the lamps the real world was already awake and bathed in a beautiful early …

Carnation, Lily, Lily, Rose

Carnation, Lily, Lily, Rose Carol V. Davis (bio) after a painting by John Singer Sargent is not the first paintingI was afraid of.That distinction belongsto Hieronymus Bosch,a melon round head leeringwith a pumpkin's grin.The body of a lion marriedto the feet of a crow. It is Carnation, Lily, Lily, Rosethat frightens me still.Others take comfort in the innocenceof the mirrored girls in white, ruffled collars.Those lanterns are not to be trusted;the orange glow in their belliesa beacon to the other side. You can tell me how foolish I am.It won't be the first time,but who's to say the white liliesare not omens of death, the one girla ghost of the other? [End Page 115] Carol V. Davis Carol V. Davis is the recipient of the T. S. Eliot Prize for Poetry from Truman State University Press. Her book Into the Arms of Pushkin will be published this fall. She is the author of two chapbooks, Letters from Prague and The Violin Teacher. She has twice been a Fulbright scholar in Russia, where her full-length collection, It's Time to Talk About . . . was published in a bilingual edition. Copyright © 2007 University of Nebraska Press

In Lesotho

In Lesotho