The mortality and morbidity of HIV-infected patients have declined with the advent of highly active antiretroviral therapy (HAART), but its effect on Candida infections remains elusive. The aims of the present study were to determine the effect of HAART on the phosphor lipase, proteinase and haemoltyic activities of oral Candida albicans, and to investigate the correlation between these enzyme profiles and the patients’ demographic and clinical parameters. Isolates were obtained from 16 Chinese HIV-infected patients on HAART and 16 healthy controls using oral rinse technique. The isolates were tested in vitro for phospholipase, proteinase and haemolytic activities using three different plate assays. The phospholipase and haemolytic activities were found to be significantly higher (p <0.05) and the proteinase activities were lower in the test group (p< 0.05). The lower proteinase activities in HIV-infected group could be due to the direct effect of protease inhibitors in HAART while the higher phospholipase and haemolytic activity in the HIV-infected group remains elusive. These finding provide evidence of altered extracellular enzyme activities in the Candida isolates obtained from HIV patients on HAART, which may affect the infectivity of the Candida isolates.