Oral Rinse Technique Research Articles

Relationship between dental fluorosis, dental caries and salivary levels of Streptococcus mutans.

Worldwide, dental caries is an endemic infection and a significant public health problem. Fluoride reduces caries by helping to prevent demineralisation, by remineralising early carious lesions, and by decreasing the activity and growth of Streptococcus mutans (S. mutans). Fluoride intake above the optimal levels leads to a condition known as dental fluorosis, which causes hypo-mineralisation of the tooth. Some studies have revealed that the severity of fluorosis is directly proportional to caries, but some showed opposite results. Hence, with these contradicting results, this study was undertaken to identify the relationship between different grades of dental fluorosis, dental caries and the most common cariogenic bacteria S. mutans in saliva. A total of 90 subjects within 14- to 16-year age range were selected and categorised depending on the severity of fluorosis into three groups: group I (mild), group II (moderate) and group III (severe) based on modified Dean's fluorosis index criteria (1942). Unstimulated whole saliva samples were collected using the oral rinse technique and processed for quantification of S. mutans using Mitis Salivarius Bacitracin Agar medium. The number of colony-forming units (CFUs) was determined using a colony counter and expressed as ≥102, ≥103, ≥104 and ≥105 CFUs per ml of the sample, in accordance with the scale recommended by the manufacturer (HiMedia Laboratories). The severity of the caries was measured using decayed, missing or filled teeth (DMFT). Observations revealed that the overall DMFT was directly proportional to the level of S. mutans titres. It was observed that 67 to 73% of participants showed fewer colony counts (102 and 103) with minimal DMFT scores and 27 to 33% showed higher counts (104 and 105) with higher DMFT scores in all the groups. 105 CFUs of group III showed the highest mean DMFT scores (2.9) with an increased number of involved subjects than group II (2.3) and group I (1.5). In all, male participants had higher bacterial titres and DMFT scores than females. Fluoride in the drinking water served as anticariogenic agent regardless of the severity of fluorosis. Severe fluorosis with a higher incidence of caries and increased CFUs of S. mutans clearly indicates the importance of preventive measures and early treatment to reduce the severity of fluorosis and prevalence of dental caries.

Prevalence of Candida albicans Infection in the Oral Cavity among Dental Clinic Patients in Taiz City, Yemen

Oral candidiasis is one of the most common oral disease of human caused by Candida albicans, and can cause superficial infection. Objective: The present study was done to evaluate the prevalence of Candida albicans infection in the oral cavity among dental clinic patients in Taiz city, Yemen. And determining the sensitivity of the isolated Candida albicans to some antifungal drugs and plant extracts. Methods: A total (200) specimens with oral candidiasis have been collected from dental patients by using the oral rinse technique, from October 2021 to January 2022. Diagnosed by dentist of clinics and hospitals, and identified by microbiological procedures. Results: Most of the isolates were Candida albicans representing in 101(50.5%) of total oral sample isolates. The non-Albicans species are the second most common representing in 51(25.5%). The remaining oral sample isolates are of non-yeast growth representing in 48(24%). Fluconazole and Itraconazole have been tested against 20 isolates of C. albicans. All the drugs, examined in vitro, show antifungal sensitivity that shows (80%). Plant extracts of Salvadora persica and Commiphora myrrha have been tested against 20 isolates of C.albicans. Four concentration of alcoholic extracts for investigated plants show antifungal activity in vitro better than aqueous extracts. Conclusions: Candida albicans were the most prevalent isolate from oral cavity of dental patients with oral candidiasis. Plant extracts of Salvadora persica and Commiphora myrrha show antifungal activity in vitro.

Is Oral Candida associated with Dental Caries in Children - A Cross Sectional Analysis.

BACKGROUND: Dental caries is a world-wide public health issue and is considered the most common of all non-communicable disease. There has been a growing interest in association between oral fungal flora especially candida and dental caries. Hence the aim of the study was to assess the correlation between active caries status and oral candida carriage in children aged twelve years.MATERIALS AND METHODS: This is a cross-sectional study where a multiphase sampling was done and a total of thirty children were selected randomly among children screened previously. Data was collected through clinical oral examinations and structured interviews. The oral rinse technique described by Samaranayake et al. was used for extracting candida spp. The correlation between candida spplevel and caries experience was performed by the Pearsons correlation coefficient.RESULTS: The mean DMFS and defs scores of the children were 3.70±4.61 and 5.83±5.51 respectively. Significant association with decay scores for deciduous teeth (P<0.05) and defs (p= <0.001) was demonstrated. Candida species was associated highly with caries prevalence and significant positive correlation was found between Candida (CFU/plate) and defs scores (r=0.790) (p= <0.001)CONCLUSION: The study results suggested that there is a significant positive correlation between candida carriage and caries, especially in children in the age group of 12 years.

Effect of statin therapy on oral Candida carriage in hyperlipidemia patients: A pioneer study.

Hyperlipidemia affects about 25% of the adult population globally. Statins are the most commonly used drugs in the management of hyperlipidemia. Laboratory and retrospective clinical studies have observed the inhibitory effects of statins on the growth of different Candida species. The effect of statin therapy on oral Candida carriage has not yet been investigated. This pioneer controlled study aimed to assess and compare asymptomatic oral Candida carriage in a group of 50 hyperlipidemic patients who were on regular statin therapy (HLS group) and in a control group of 50 subjects, matched in terms of gender, age and dental status, who were hyperlipidemic, but not on statin therapy (HLNS group). The patients were recruited from the outpatient clinics of 2 university hospitals. The concentrated oral rinse technique was used to isolate oral Candida species in both groups. Candida species were identified using the germ tube test and the VITEK® 2 system. The Candida prevalence and colony count were significantly lower in the HLS group as compared to the HLNS group (n = 20, 40% vs. n = 30, 60%, respectively; p = 0.040). There was no significant difference in the oral Candida prevalence or colony count between different age groups in either the statin or control subjects. Statin therapy is associated with a reduction in oral Candida carriage in both prevalence and the colony count in hyperlipidemic patients.

Non-Invasive Saliva-based Detection of Gene Mutations in Oral Cancer Patients by Oral Rub and Rinse Technique.

Background:Oral Squamous Cell Carcinoma (OSCC) is the most widely reported cancer worldwide. Liquid biopsy, a method that relies on identification of tumor-associated cells and/or cell free nucleic acids from body fluids is becoming increasingly popular in cancer diagnostics. The aim of the study was to evaluate the feasibility of Oral Rub and Rinse (ORR) technique in determining the genetic changes in common biomarkers of oral cancer such as TP53 using DNA obtained from saliva of oral cancer patients. Methods:A total of 15 oral cancer patients were recruited in the study and pre-surgical saliva samples were collected using the ORR technique. Tissue samples included in the study were obtained during the surgical excision of the cancerous oral lesion. Genomic DNA was isolated from the salivary cell plug and the tissues and the TP53 gene was amplified by PCR. The PCR products of all the exons of TP53 (Exons 2 to 11) were electrophoresed on agarose gel, purified and sequenced by Sanger method. The obtained sequences were compared with the reference sequence of TP53 gene. Statistical analysis used:Descriptive statistics were used and reported as frequency and percentage. Results:Capillary sequencing of TP53 gene from tissue DNA revealed the presence of codon 72 c.215C>G (p.Pro72Arg) polymorphism in 10 patients (67%) and a heterozygous mutation at codon 172 c.514 G>T (p.Val172Phe) in 2 patients (13%). Among the 10 samples that showed codon 72 polymorphism, matched salivary DNA was available for 6 samples and 4 out of these showed same genetic change at codon 72. Similarly, of the 3 samples that showed codon 172 mutation, matched salivary DNA was available for 1 sample and the mutation status was identical. Conclusion:The results suggest a potential for clinical applications of ORR technique as an alternative to invasive tissue biopsy for detection of genetic changes in candidate biomarkers in oral cancer.

Relationship of Clinical Features with Candidal Carriage in Oral Submucous Fibrosis Patients: A Case-control Study.

Background and Objectives:Oral submucous fibrosis (OSF) is extensively prevalent in India and South-East Asia owing to the habit of arecanut (AN) use. Epithelial atrophy, hyposalivation, and immune alterations in OSF may predispose to increased Candidal carriage. Stomatopyrosis in OSF can result from multiple causes, which may also include Candidal infection. Hence, this study is aimed to assess Candidal carriage, species characterization, salivary flow rate (SFR) and its relationship with the clinical features (stomatopyrosis and mouth opening [MO]) in OSF patients alongwith the response to antifungal treatment in patients with higher Candidal carriage.Methodology:In this case-control study, 60 OSF patients and 30 age- and sex-matched control subjects were enrolled. SFR was assessed using modified Schirmer test. Samples for Candidal assessment were collected with the oral rinse technique and cultured. The isolated yeast species were counted and identified based on Gram staining, germ tube test, and CHROMagar. Data were analyzed with Chi-square test, Pearson's correlation test, and one-way ANOVA test.Results:The distribution of mean visual analog scale (VAS) score, SFR and MO was significantly varied (P < 0.001) in the study and control groups. Candida was found to be present significantly (P = 0.048) in OSF group as compared to control group. Candida albicans was the predominant species. No statistically significant association was obtained regarding Candidal isolation and SFR, burning sensation and MO in OSF patients. Only 1 patient in the study group yielded a high Candidal carriage (>400 CFU/mL) and reported relief in burning sensation (VAS score) with antifungal therapy.Conclusions:OSF patients yielded a significant higher oral Candidal carriage. Although it was not found to be associated directly, its role as a “cause and effect” in SFR and clinical features (stomatopyrosis and MO) of OSF cannot be ignored.

Candida Prevalence in the Saliva of Controlled and Uncontrolled Diabetic Patients – A Clinical Study

Context: Diabetes mellitus is a major cause of mortality and morbidity worldwide. The health of oral tissue is related to saliva and both the composition and flow of saliva are altered in diabetic patients. Aim: This study aims to assess the prevalence of Candida in diabetic patients and non-diabetic controls and to assess the relationship between the candidal carriage and glycemic control, the relationship between the oral prevalence of Candida species, and clinical candidal infection, and the effect of antidiabetic therapy and smoking on candidal infection. Settings and Design: This cross-sectional population-based study was conducted on 100 diabetic and 50 non-diabetic (control) patients. Methods and Material: Saliva samples were collected and the concentrated oral rinse technique was used for quantitative oral candidal isolation. A digital colony counter enumerated several candidal colonies on each plate. Statistical Analysis Used: Chi-square test and Mann–Whitney test are used. Results: Colony-forming units (CFU/ml) in diabetics were 3131.14 and in control was 986.8. Out of 55 diet control patients, 30 had a candidal carrier; out of 44 patients on oral hypoglycemic, 30 had candidal carrier; and out of 1 patient on insulin, 1 had a candidal carrier. Out of 19 diabetics (good control), 8 had candidal carrier; out of 28 (moderate control), 12 had candidal carrier; and out of 53 (poor control), 41 had a candidal carrier. Out of 14 smokers, 8 had candidal carriers, and out of 36 nonsmokers, 11 had candidal carriers. Conclusions: Diabetic patients carry a higher number of candida in their oral cavity.

Oral candidal carriage and its association with dental carious lesions in asymptomatic adults: a cross-sectional study from the UAE

BackgroundCandida’s potential association with dental caries has previously been reported in children. This research aimed at investigating the associations between Candida species in whole saliva and dental and periodontal health indices in a sample of adult patients.MethodsA cross-sectional study involving 160 patients investigated the associations between Candida species collected by oral rinse technique, and Decayed, Missing, and Filled Surfaces (DMFS), and periodontal health indices. Chi-square and Independent Samples t-test were used to assess the associations between Candida carriage and confounding variables. Binary logistic regression was used to assess association strengths between Candida carriage and DMFS, adjusted for socio-demographic variables, diabetes mellitus and plaque index.ResultsCandida colonies were identified in 49 (30.6%) patients with CFUs ranging from 103 to 105 colonies per mL. The quantity of Candida CFUs increased with age (r = 0.200; p < 0.05). Among all dental and periodontal health indices, only DMFS was significantly associated with higher values of Candida carriage (p = 0.034), and this association was independent from sex, age, smoking, diabetes mellitus and plaque index (OR: 1.014; 95% CI 1.0–1.03; p = 0.048).ConclusionThe association between Candida species in whole saliva and DMFS reported here supports an etiological role of Candida in dental cariogenesis among adults.

Antifungal Efficacy of Wrightia tinctoria (Roxb.) R.Br on Candida Species Isolated from the Oral Cavity

Introduction: Nature is a valuable source of active ingredients that needs to be explored, especially its utilization in the medical field. Owing to the limited availability of antifungal drugs and also considering their side effects, there is always a constant need for a safe and competent alternative. Wrightia tinctoria, a medicinal tree, has been reported to possess potent antifungal activity against commercially available candida strains [American type culture collection (ATCC) and microbial type culture collection (MTCC)]. This could be beneficial clinically only if its antifungal activity could be proved against candida species isolated from clinical samples as the commercially available candida strains might have lost its pathophysiological characteristics on repeated subcultures. Hence, with this background, we performed this study to determine the antifungal efficacy of the extracts obtained from the leaves of W. tinctoria against the candida species isolated from the oral cavity. The aim of this study was to determine the antifungal efficacy of W. tinctoria on candida species isolated from the oral cavity. Materials and Methods: To determine the antifungal activity, acetone, ethyl acetate, and chloroform extracts of leaves of W. tinctoria were used. The study population consisted of five healthy volunteers above 18 years of age without any harmful habits and five patients at increased risk of candida infection. Saliva samples were collected by oral rinse technique using phosphate buffered saline. Fluconazole was used as a positive control and the antifungal efficacy was determined using disk diffusion method. Kruskal-Wallis test was used to determine the significant difference between the extracts. Results: The mean zone of inhibition of acetone, ethyl acetate, and chloroform extracts of leaves of W. tinctoria was 10.8667, 11.0000, 10.1333 mm, respectively. Conclusion: Acetone, ethyl acetate, and chloroform extracts of W. tinctoria possess antifungal activity against candida species isolated from oral cavity.

IDENTIFIKASI FAKTOR RISIKO TERJADINYA KOLONISASI SPESIES CANDIDA DI RONGGA MULUT PADA INDIVIDU SEHAT (PENELITIAN PADA MAHASISWA &amp; MAHASISWI FKG UI)

Introduction : Cases of oral candidiasis are commonly found, both in healthy individuals and immunecompromise patients, however publications of Candida carrier in the oral cavity of healthy population and risk factors for colonization in Indonesia are hardly available. Objective : This study was aimed to analyze the type and number of Candida colonies and identify risk factors in the oral cavity of apparenthly health FKG UI students. Material and methods : the specimens were taken from 195 subjects with oral rinse technique for identification using culture medium CHROMagar® and Sabaraoud dextrose agar. Results and discussion : Candida species were found in the 107 subjects oral cavity (54.87%), being Candida albicans was is the predominant species (52.33%). Some 88 subjects (82.24%) was dominant in the number of colonies less than 400 CFU/ml, while the rest had colony of more than 400 CFU/ml (17.76%). Candida colony grew dominantly in single colony (90.65%), and the others showed multi-species colonies (9.34%). Risk factors identified included age; gender; hormonal; blood type O; denture; orthodontic appliances; unstimulated salivary flow; pH of saliva; smoking, alcohol and oral cleaning habit; and oral health status. By using a statistical Pearson chi-square test, no significant relationship was found between risk factors and number of Candida colonies in the oral cavity p less than 0.05. Conclusion : there was no one single risk factor for Candida colonization, but combination of various risk factors for demographis, local and systemic was observed

Comparison of antifungal efficacy of ethanolic extracts of Woodfordia fruticosa leaf and Punica granatum peel in uncontrolled diabetic patients wearing removable dentures: A randomized controlled clinical trial.

Background and Purpose : The search for the development of a suitable novel antimicrobial agent for fungal diseases continues to be a key problem in the current clinical field. The present investigation was aimed to determine the antifungal effect of the ethanolic crude extracts of Woodfordia fruticosa leaf (Wfl) and Punica granatum peel (Pgp) in uncontrolled diabetic patients wearing removable dentures.Materials and Methods: The ethanolic extracts of both plants were prepared using the soxhlet extraction method, and the obtained metabolites were confirmed by thin- layer chromatography. After the preparation of the mouthwash, a total of 100 subjects were randomly divided into two groups. Each subject was given physiological saline at the baseline. Group I was provided with P. granatum mouthwash, while Group II was given W. fruticosa mouthwash. Following the administration of the mouthwash, the patients were requested to rinse the mouthwash using the oral rinse technique twice daily 5 ml/rinse for 30 sec. Subsequently, colony-forming units (CFU) were evaluated in the participants. Post-therapeutic samples were collected 1 h and 1 week after the mouthwash use.Results: The mean reduction of CFU was calculated at the baseline, as well as 1 h and 1 week after using mouthwash. The results indicated a drastic reduction in CFU 1 h and 1 week after the application of Wfl mouthwash.Conclusion: The obtained data revealed that Wfl had potential anticandidal activity against Candida yeast cells, probably owing to its bioactive compounds like glycosides. Therefore, this agent can be used effectively as a natural remedy for the treatment of oral candidiasis. However, the exact mechanism of action of this plant needs to be elucidated.

Oral Candidal carriage and associated risk indicators among adults in Sakaka, Saudi Arabia

BackgroundCandida is a ubiquitous organism in nature which inhabits the oral cavity as part of the normal microbial flora. The oral carriage of Candida is perpetuated by several predisposing factors.MethodsThis cross-sectional study was designed to investigate the carriage rate of Candida among 104 voluntary adults at the college of medicine - Jouf University. The concentrated oral rinse technique using Sabouraud Dextrose agar medium supplemented with 0.05% Chloramphenicol was used to isolate Candida. The relative factors affecting the colonization of Candida and the concentration of each type were also determined.ResultsCandida species were isolated from the oral cavity of 45 (43.4%) subjects. Of these 55.6% were identifies as C. albicans as determined by the Vitek 2 compact system. Other Candida species were represented by C. glabrata (11.1%), C. krusei (11.1%), C. dubliniensis (8.9%), C. parapsilosis (6.7%), C. tropicalis (4.4%), and C. famata (2.2%). Subjects with very poor plaque status, severe gingivitis and diabetes had significantly (P = 0.001) high concentration of Candida spp.ConclusionPlague, severe gingivitis, and diabetes were found to be significantly associated with higher Candida colonization.

The relationship between Candida albicans colonization and oral hygiene in cancer patients undergoing chemotherapy

Abstract Various Candida species are harmless commensals present in the oral mucous membranes, and Candida albicans (C. albicans) is the most commonly isolated species. Overgrowth of C. albicans can lead to oral fungal infections, known as oral candidiasis, with multiple local and systemic predisposing factors. Poor oral hygiene (OH) is an important local factor that may increase the risk of oral candidiasis. An immunocompromised patient, such as cancer patient undergoing chemotherapy, has an increased susceptibility to develop oral candidiasis due to an impaired or weakened immune system. The aim of this study was to evaluate C. albicans colony forming units (CFUs) and its relationship with different levels of oral hygiene in cancer patients undergoing chemotherapy. The oral rinse technique was performed to isolate C. albicans from 60 subjects and were cultured on agar plates. Subjects were divided into different groups of OH level according to OHI-S scores. C. albicans were isolated from 44 (73.3%) subjects. There is a strong positive relationship between OH status and CFU ml-1 value (r = 0.708). It showed that oral C. albicans colonization increased significantly in cancer patients undergoing chemotheray with fair and poor OH (p = 0.001). Data from this study revealed a statistically significant relationship between oral hygiene status and C. albicans colonization among cancer patient undergoing chemotherapy. More attention should be paid to oral hygiene as one of the risk factors to be taken into account in the prevention paradigm to reduce the incidence of oral candidiasis among cancer patient undergoing chemotherapy. Further studies with larger sample sizes would be beneficial with the aim of verifying the results obtained in the present study, and consider the interaction effects of other multiple risk factors on the risk of developing oral candidiasis in cancer patients.

Targeted Screening Programme For Early Detection Of Oral Malignant And Potentially Malignant Lesions Using The Oral Rub And Rinse Technique Among Truck Drivers in Dakshina Kannada District − A Cross-Sectional Study

Introduction: Early detection of oral malignant and potentially malignant disorders is very important in reducing morbidity and mortality. Truck drivers are transport workers who transport goods over long distances in trucks are at a very “high risk” due to various factors. The aim of this study was to detect potentially malignant and malignant oral lesions using the oral rub and rinse technique among truck drivers in Dakshina Kannada. Materials and Methods: An oral cancer screening program among truck drivers was conducted in Dakshina Kannada district, Karnataka. Data regarding socio-demographic details, history of tobacco use were collected. Oral cancer screening was carried out. Exfoliative cytology was done using the oral rub and rinse technique if red/white lesions were present and biopsies were taken in case of ulcerated lesions. Descriptive statistics was used in this study in the form of number and percentage. Results: A total of 964 subjects were screened for potentially malignant and malignant oral lesions. Most of the patients (83%) were current tobacco users. Of the 964 subjects, 148 had red/white lesions. Results of exfoliative cytology showed that 23% smears were Class I, 24% were Class II, 31% were Class III, and 16% were Class IV smears; 6% smears were unsatisfactory. Tissue biopsy was taken for 23 patients with ulcerated lesions − 2 were confirmed for oral squamous cell carcinoma and 21 had dysplasia. Conclusion: High-risk targeted screening for oral cancer can help identify oral precancerous lesions at an early stage.

Chronic nail biting, orthodontic treatment and Enterobacteriaceae in the oral cavity.

BackgroundChronic nail biting is common in children and young adults. Auto inoculation of environmental pathogens can manifest as infection in distant organs. Multi-drug resistance gram negative bacteria are on the rise globally. Several of the foodborne bacteria fall within the Enterobacteriaceae family but very few studies have explored these microbes in the oral cavity of children with chronic nail-biting habit or orthodontic treatment. The study aims to investigate oral load of Enterobacteriaceae in children with chronic nail-biting habit and or those undergoing orthodontic treatment.Material and Methods150 children (no nail-biting n=30, nail biting n=60, fixed orthodontic treatment n =30 and a combination of fixed orthodontic appliance use and nail-biting habit n =30) were assessed for culture based microbiological investigation. The concentrated oral rinse technique was used. The rinse was inoculated in MacConkey’s and Blood Agar. The gram stained culture was subjected to biochemical tests for sub-species identification using Biomerieux Vitek 2 Compact Automated Microbiological Analyzer. Fisher’s exact and Kruskal Wallis with post hoc analysis using Dunn method was performed to test association and difference between groups.ResultsEnterobacteriaceae was positive for 72% of the children. Of them, nail biting or orthodontic treatment group comprised 89%. Those with a combination of nail biting and undergoing orthodontic treatment exhibited highest CFU/ml and those without nail biting or orthodontic treatment exhibited the lowest. Three of the four organisms isolated tested positive in the orthodontic treatment group. E. coli was positive in 38% of the children while Klebsiella and E. cloacae were isolated exclusively in the orthodontic treatment group.ConclusionsChronic nail biting or the use of fixed orthodontic appliances is associated with higher incidence of Enterobacteriaceae in the oral cavity. Oral health professionals play an important role in preventing multi drug resistance infectious diseases. Key words:Enterobacteriaceae, nail-biting, Onychophagia, orthodontic treatment.

Effectiveness of the 'Oral Rub And Rinse Technique' As a Tool for Oral Cancer Screening in South Indian Population

Background: Oral cancer is a public health problem and causing huge economic and a social burden. Early detection of oral potentially malignant disorders and oral cancers is very important in reducing the morbidity and mortality rates. Aim: To assess the effectiveness of the 'Oral Rub and Rinse Technique' as a tool for oral cancer screening among South Indian population. Methods: A population based, high risk targeted oral cancer screening was conducted in 2 districts of Karnataka, South India. About 523 subjects participated in the study. Data were collected regarding the sociodemographic details, history of tobacco use and knowledge regarding oral cancer. The participants were then screened for presence of oral premalignant/malignant lesions or conditions. Exfoliative cytology was done using traditional method and the 'Oral Rub and Rinse Technique' if red/white lesions were present. Smears were stained with Papanicolaou (PAP) stain and were evaluated by 2 cytopathologists independently. The data were tabulated and subjected to statistical analysis. Results: Of the 523 subjects, 112 had red/white lesions. Of these, 23% were Class I smears, 24% Class II smears, 31% Class III and 16% Class IV smears and 6% were unsatisfactory using the conventional technique whereas the Oral Rub and Rinse Technique showed 19% Class I smears, 28% Class II smears, 32% Class III smears, 17% Class IV smears and 4% unsatisfactory. The cellular quantity and quality in terms of clarity and dispersion was better with the smears prepared by the Oral Rub and Rinse Technique than the conventional technique. Conclusion: Oral Rub and Rinse Technique can be a reliable tool for population screening of oral cancer.

Oral Candida colonization in patients with chronic periodontitis. Is there any relationship?

BackgroundCandida can be implicated in the pathology of chronic periodontitis. AimsTo analyze the oral Candida carriage in patients suffering from chronic periodontitis (CP) and its correlation with the severity of this condition. MethodsMicrobiological samples were taken from 155 patients using the oral rinse (OR) technique and by using paper points in the periodontal pockets (GPP). These patients were divided into 3 groups: 89 patients without CP (control), 47 with moderate CP, and 19 with severe CP. Samples were cultured in a Candida chromogenic agar for Candida. Species were identified by microbiological and molecular methods. ResultsCandida was isolated in the OR of 45 (50.6%), 21 (44.7%), and 11 (57.9%) patients, respectively, and in the GPP of 32 (36%), 14 (29.2%), and 10 (42.6%) patients from the control, moderate CP and severe CP groups, respectively. Candida was isolated more frequently and in a greater burden in OR than in GPP (p<0.01). Candida albicans was the most prevalent species. GPP of patients with CP had poor fungal biodiversity (p<0.01). ConclusionsColonization by Candida was present in the samples of patients without CP, and with both moderate and severe CP. Nonetheless, patients with severe CP had a higher rate of Candida colonization, especially by C. albicans.

Relationships of Candida with Frequency of Brushing, Age and Smoking in Patients with Removable Dental Prosthesis: A Quantitative Study

Objective: Although progress has been made to reduce opportunistic infection of fungi in the oral cavity, the prevalence of denture stomatitis associated with Candida has increased. The purpose of this study was to evaluate the effect of denture cleaning frequencies, age and smoking habit on the levels of Candida species in the saliva of patients wearing removable partial or complete dentures. &#x0D; Methods: Subjects wearing removable partial or complete dentures were recruited. A questioner was used to collect information on oral hygiene habits and smoking. Saliva samples were collected by oral rinse technique in a sterile container and cultured in duplicate Sabouraud Dextrose Agar. The numbers of colonies were determined by aCOLyte colony counter and the number expressed as a colony forming unit (CFU). The CFU and clinical data were analyzed for correlation and the Kruskal-Wallis test was used to determine statistically significant differences. &#x0D; Results: Among 99 subjects recruited, 47 were wearing complete dentures and 28 were smokers. The brushing frequencies were once (37 subjects), twice (39 subjects) and three times (23 subjects) per day. CFU was significantly higher in partial denture wearers than complete denture wearers and the brushing frequencies significantly correlated with CFU (r = -0.85, P= 0.001). There was no statistically significant difference in CFU between smokers and non-smokers and no correlation of CFU with age was found. &#x0D; Conclusions: This quantitative study has suggested that there are statistically significant differences in the levels of Candida in the saliva of subjects with different brushing frequencies and wearing a different type of denture. However, no statistically significant difference was noticed between smokers and non-smokers, and there was no significant correlation between CFU and age.

Evaluation of Antifungal Efficacy of Ethanolic Crude Lawsone and Listerine Mouthwash in Uncontrolled Diabetics and Denture Wearers - A Randomized Clinical Trial.

Lawsonia Inermis (LI) is a shrub cultivated throughout India. Many in vitro studies have been done on antifungal activity of LI, although none of the studies have been conducted invivo. To evaluate the antifungal efficacy of ethanolic extract of crude lawsone in comparison with listerine mouth wash in known diabetics and wearing dentures. A total of 60 subjects were taken and randomly divided into two groups of 30 each. Group 1 received crude lawsone mouthwash and Group 2 received listerine mouth wash. Oral rinse technique was performed. Each subject was given distilled water at baseline and Colony Forming Units (CFU) of candidal species was determined. Post therapeutic samples were then collected 1hr and 1week following drug usage and they were further advised to use given mouth washes twice daily with volume of 5ml/rinse for 30 seconds and CFU was evaluated. Crude lawsone mouthwash showed superior antifungal activity when compared to listerine mouthwash. On individual comparison of both mouth washes at baseline, 1hr and 1week highly significant results were obtained using inferential statistics. The inter group comparison was done using independent t-test where lawsone was considered to be more effective in reducing CFU, at 1hr and 1week of using the mouth wash (p<0.01). Subjective symptoms like taste and smell were determined by chi square test where good taste was felt for lawsone and olfactory satisfaction was good with listerine (p<0.01). Burning sensation was found to be more with listerine mouth wash. The present study revealed superior antifungal activity with ethanolic extract of crude lawsone mouth wash compared with listerine mouthwash.

A new approach to exfoliative cytology: A comparative cytomorphometric study.

Early detection of oral premalignancy and malignancy using simple screening aids play a promising role in curbing the disease. The primary aim of this study is to evaluate and the secondary aim of this study is to compare the cytomorphometry and cellular atypia in keratinocytes obtained from oral rinse and conventional exfoliative cytology in normal oral mucosa and clinically diagnosed oral leukoplakia. The study comprised of 55 clinically diagnosed cases of leukoplakia and 55 age and sex matched normal controls. Smears were prepared using oral rinse technique followed by the conventional exfoliative cytology. Papanicoloau stained smears were evaluated for atypia and subjected to image analysis. Based on the presence of atypia they were further divided into three groups (Group 1-cases with atypia, Group 2-without atypia and Group 3-normal controls) and analyzed. Statistical analysis used one-way analysis of variance followed by Tukey Honestly Significant Difference test for intergroup analysis and unpaired students t-test to compare the two methods. Smears prepared with both methods demonstrated atypia in 18 cases. The cellular diameter and cellular area (CA) were progressively increased from Group 1 through Groups 2 and 3 in both the smears. Nuclear diameter and nuclear area and nuclear cytoplasmic ratio progressively decreased from Group 1 through Groups 2 and 3. Both the methods showed no significant differences among the cellular parameters except in normal controls. Cytomorphometric analysis of keratinocytes obtained with oral rinse method and wooden spatula can serve as a useful screening aid to detect oral leukoplakia. Oral rinse method being more convenient results in smears of better quality.