Objective To explore the effect of negative pressure attraction method of brushing and washing apply in oral care of patients with orotracheal intubation.Methods Totals of 82 oral intubation patients in ICU were randomly selected and divided into experience group ( n =42 ) and control group ( n =40 ).The traditional method of oral care was used in control group,while the negative pressure attraction method of brushing and washing was used in experience group,then,the incidence of oral infections,ventilator-associated pneumonia( VAP),dental plaque and oral mucosal injury were observed in two groups.Results The incidence of oral infections,ventilator-associated pneumonia(VAP),dental plaque in experience group respectively were 7.14%,2.38%,4.76%,and that in control group were 22.50%,15.00%,32.50%,the differences were statistically significant ( x2 =3.88,4.18,10.55,respectively; P < 0.05 ),while there was no significant difference in the incidence of oral mucosal injury between two groups ( P > 0.05 ).Conclusions The negative pressure attraction method of brushing and washing can effectively keep the oral clean of patients with orotracheal intubation,reduce the incidence of oral infections and ventilator-associated pneumonia and dental plaque,when compared with the traditional method of oral care. Key words: Oral cavity; Care; Orotracheal intubation; Negative pressure attraction method of brushing and washing