Aim: The aim of this study was to assess the awareness and knowledge of the dental students towards oral biopsy as a diagnostic tool in relation to oral lesions. Background: Biopsy is defined as the removal of tissue from the living organisms for the purpose of microscopic examination and diagnosis. Clinician have to decide the type of biopsy required based on the site, clinical nature of the lesion, and proximity to vital structures. General dentists must be able to perform simple oral biopsies for the diagnosis of oral lesions. The capacity to differentiate between benign and premalignant or malignant oral lesions is essential for establishing a correct diagnosis. Materials and Methods: The survey was cross-sectional in design. Following participant self-administered enrolment in the study, data were collected by questionnaire that assessed participants’ knowledge on oral biopsy as a diagnostic tool in relation to oral lesions. A total of 100 questionnaires were disturbed to dental students of Saveetha Dental College, Chennai, Tamil Nadu. Results: The survey was completed within two weeks from the start of the study. Overall response rate was 100% and all completed the questionnaire (100 participants) within their stipulated time. The overall knowledge of the study participants was assessed based on their responses to the questionnaire. This study reveals that the dental students of Saveetha Dental College have a decent and fair general knowledge towards Oral Biopsy. The various ways to improve awareness regarding Oral biopsy as a diagnostic tool in relation to oral lesions are by conducting continuing medical education (CMEs), Workshops, Training students, etc.