Oral Administration Of Methyltestosterone Research Articles

The effects of oral administration of methyltestosterone on seminal vesicle development, in African catfish

The effects of oral administration of methyltestosterone on seminal vesicle development, in African catfish

The effects of methyltestosteone on reproductive function in male greyhounds

Oral administration of methyltestosterone (MT) at 50 mg/dog/day to intact adult male greyhounds for 90 d resulted in decreased (P < 0.05) mean daily sperm output and mean testicular length. Additionally, the mean diameter of seminiferous tubules in MT-treated dogs tended to decrease (P = 0.08). Mean concentrations of luteinizing hormone (LH) and follicle stimulating hormone (FSH) and concentrations of testosterone in serum were also decreased or tended to decrease (P = 0.0003 to 0.059) at various sampling periods during MT treatment, suggesting alterations in spermatogenesis resulted from decreased serum concentrations of gonadotropins and steroids. Mean daily sperm output, mean testicular length, mean seminiferous tubule diameter and mean concentrations of FSH in serum were not decreased (P > 0.05) at the end of a 90-d recovery period. However, mean concentrations of serum LH and concentrations of testosterone were still lower (P < 0.05) during five of six and one of six sampling times, respectively, during the recovery period than the pretreatment levels, suggesting a prolonged effect of MT treatment on the pituitary/gonadal axis.

Induced sex reversal and spawning of blue-spotted grouper, Epinephelus fario

Attempts were made to induce sex reversal of under 2-year-old blue-spotted groupers and to induce spawning of 4-year-old females. Mature males, through sex reversal, were obtained successfully by the oral administration of methyltestosterone (MT) at daily feeding doses of 0.5 mg and 1.0 mg MT/kg body weight for 5 months. The sperm concentration was 8×10 7 cells/ml milt. The 2-year-old fish attained a sexually mature state in June and July with an average gonadosomatic index (GSI) of 2.07%. Changes in GSI and serum profile of estradiol-17β and 17α-testosterone were correlated with stage of gonad development, including vitellogenesis, sex transition and spermatogenesis. The values of GSI, serum estradiol and testosterone were elevated towards the completion of vitellogenesis and depressed during the sex transition phase. An increase in sex steroid concentrations, testosterone in particular, was recorded during spermatogenesis. Serum testosterone concentrations, ranging between 150 and 200 pg/ml serum in the prespawning season, gradually increased as vitellogenesis progressed and reached a level of 633 pg/ml serum in the mature females during the spawning season. Under the influence of MT administration, serum testosterone concentrations increased to 1100 pg and 1630 pg/ml serum at the end of a 170-day period at daily feeding doses of 0.5 mg and 1.0 mg MT/kg body weight, respectively. Spawning was induced by multiple injections of human chorionic gonadotropin (Puberogen) at doses of 2000–3000 IU HCG/kg fish. The frequency of hormone injections depended upon the stage of sexual maturity, as indicated by the stage of oocyte development and initial oocyte diameter. Two injections, 24 h apart, were found adequate when the recipient females contained mature oocytes at the tertiary yolk globule stage and a mean diameter of greater than 0.55 mm. For fish containing oocytes in the range of 0.50 to 0.55 mm, three injections were required to achieve successful spawning.

Zinc nutritional status, androgens, and growth retardation.

Zinc levels were measured in hair and serum of boys with constitutional growth delay and familial short status and in several boys before and after oral administration of methyltestosterone. These results show the following: (1) zinc levels in boys beyond stage 3 of genital development are significantly higher than in stage 1 and 2; (2) there is a linear relationship between zinc levels and serum testosterone concentration (up to 250 ng/dL); and (3) methyltestosterone administration raised the zinc concentration in serum and hair, especially in boys with constitutional growth delay. Therefore, increased endogenous production or exogenous supply of testosterone are associated with increased zinc levels. We speculate that the relative testosterone deficiency and hypogonadotropism seen in constitutional growth delay may result in decreased zinc levels, which in turn could cause a further delay in the appearance of secondary sexual characteristics and greater growth retardation.

PROCESS OF FUNCTIONAL SEX REVERSAL OF THE GONAD IN THE FEMALE GUPPY, POECILIA RETICULATA, TREATED WITH ANDROGEN BEFORE BIRTH.

In the guppy, Poecilia reticulata, ovarian differentiation occurs during the embryonal life by 14 days after the preceding parturition. Testicular differentiation begins with the appearance of prominent aggregations of stroma cells in the gonadal hilus occurring by 18 days following the last parturition. Oral administration of methyltestosterone (400 μ/g diet) to gravid guppies, begun 13-15 days after the preceding parturition and continued until the end of gestation, induced a male-type aggregation of somatic cells in the hilus of ovaries of female embryos. Gonads of newly born, androgenized females still had developing oocytes but were always provided with atypical clusters of stroma cells in their hilus. The gonads of affected female offspring developed successively into definite testes within 20 days after birth, displaying a precocious differentiation of the hilar stroma into sperm ducts and testicular interstitium, a concomitant initiation of spermatogenesis, and a conspicuous degeneration of oocytes. A successful masculinization of the somatic element, which may occur prior to that of the germ cells, in androgen-affected embryonic ovaries seems to be essential for the functional sex reversal of genetic females in the guppy.

Effects of methyltestosterone on the skin and the gonad of salmonids

Oral administration of methyltestosterone caused dark yellow coloration and a thickening of the skin in young pink salmon. This thickening of the skin was due partly to the hypertrophy of mucous cells and of cells in the stratum germinativum in the epidermis. The mucous cells became active in mucous secretion. Furthermore, the upper layer of the dermis became thick and dense and enclosed the scales after 2 weeks. Descaling was inhibited by the treatment. All ovarian eggs had degenerated and spermatogenesis was suspended. Some spermatogonia became hypertrophic and developed vacuoles in the cytoplasm. Hypertrophic changes were also observed in interstitial-like cells and in the cells lining the sperm duct, accompanied by active secretion of a liquid material. No large differences were observed between dosages of concentrations of 50 and 100μg/g diet. Similar changes were observed in chum salmon.

Effect of several anabolic steroids on sulfobromophthalein (BSP) retention in rabbits

Fasted, unanesthetized female rabbits were employed to study the disappearance of sulfobromophthalein (BSP) from blood and bile. The influence of several anabolic steroids on serum BSP at 20 minutes after single intravenous injections of BSP at 20 mg/Kg/ body weight was studied. Oral administration of methyltestosterone and norethandrolone (17α-ethyl-17β-hydroxy-4-estren-3-one) caused dose-related increases in BSP retention. Oxandrolone (17β-hydroxy-17α-methyl-2-oxa-5α-androstan-3-one) appeared to be less effective than the other two steroids in causing increased BSP retention. Testosterone propionate has no effect on BSP retention.

The Effect of Oral Administration of Methyl Testosterone on Swine Growth and Development3

The Effect of Oral Administration of Methyl Testosterone on Swine Growth and Development3

Influence of Season on Comb Development of Chicks Following Oral Administration of Methyl Testosterone

THE discovery of considerable amounts of male hormone in the feces of dairy cattle, (Riley and Hammond, 1942) raises the intriguing problem as to the possible functional activity of this hormone during pregnancy and lactation. It was considered important to determine the rate of elimination of the male hormone in individual cows during various physiological conditions. A simple biological assay method was developed consisting of feeding 10 percent dry cow manure in the chick starter ration in place of an equal amount of alfalfa meal (Turner, 1947). At 28 days of age the chicks were sacrificed, the comb cut off even with the head and weighed.To obtain an estimate of the amount of androgenic hormone present in manure, a comparison was made with the comb weight which could be stimulated by the oral administration of methyl testosterone. It was observed that chicks fed methyl testosterone at the rate of .

EXPERIMENTAL USE OF TESTOSTERONE COMPOUNDS IN PREMATURE INFANTS

IN A preliminary report Shelton and Varden (11) demonstrated that the oral administration of methyl testosterone to premature infants of both sexes, weighing less than 2000 grams, resulted in an apparent increase of expected survival, with concomitant minimal initial weight loss and early and sustained weight increase throughout the period of treatment. In an effort to obtain more conclusive data than the initial study provided, testosterone compounds were administered to a much larger group of infants on the premature wards of the Los Angeles County General Hospital, over a period of several months, according to the following procedure: All premature infants weighing less than 2000 grams were divided into three groups in rotation of entry, with no regard for sex or weight. The first group received methyl testosterone 5 mg. daily in propylene glycol, placed directly on the tongue immediately preceding an oral feeding or gavage. The second group received 4 mg. daily of testosterone propionate intragluteall...

Effect of Methyl Testosterone Treatment on Muscular Performance and the Central Nervous System of Older Men

IN AN EARLIER investigation (1) improvement of endurance in dynamic and static work, of finger ergograph performance, of the fusion frequency of flicker and, to a lesser extent, of muscular strength in four male subjects with diminished sexual hormone production (two eunuchoids, two castrates) were reported after four weeks' oral administration of methyl testosterone. An adequate level of male-sex hormone might be important for the maintenance of working capacity. If this is so, then the reduction and final cessation of the sex-hormone production with age might influence the reduction of working capacity with age. In other words, the reduction of working capacity with age might proceed differently if the sex-hormone concentration could be artificially maintained at a higher level. A very large amount of material would be necessary to prove or disprove this hypothesis. In the present paper we are reporting an investigation to determine whether exaggerated fatigue in older men might be partially due to redu...

Some Metabolic Effects of Testosterone Implants

Thorn and harrop (1) have shown that the administration of testosterone to normal dogs is followed by a decreased excretion of sodium and water, an effect resembling that of adrenal cortical extract. Kochakian and Murlin (2) found the nitrogen content of urine in castrate dogs diminished after the injection of androgens, while Thorn and Engel (3) noted a decrease in urinary nitrogen in normal dogs given testosterone propionate. These reports represent but a few of the recorded observations on animals. More recently, Kenyon et al. (4) have shown that in normal men and women, and particularly in eunuchoids, the injection of testosterone propionate ‘produced a reduction in the urinary excretion of nitrogen, sodium, potassium and chloride.’ When the injections of testosterone were discontinued, the excretion rates returned to the pre-injection levels. Jones et al. (5) noted that the oral administration of methyl testosterone possessed ‘the property of causing nitrogen, electrolyte and water retention.’

Oral Administration of Methyl Testosterone in Gynecology

THE USE OF ANDROGENIC HORMONES for the relief of varied functional gynecologic disorders has now been thoroughly accepted. That male sex hormone therapy has an established use in certain gynecologic disturbances is attested by the large number of clinical and experimental reports covering thousands of cases. The method of administration of these androgenic substances has been parenterally, either by the intramuscular injection of testosterone propionate in oil, the use of an active ointment through the skin, or more rarely, the subcutaneous implantation of pellets. The purpose of the present paper is to show that equally good results in the treatment of many gynecologic disturbances have been obtained by the oral administration of the synthetic and orally active androgenic hormone, methyl testosterone. It will be seen from the results of the present series of cases that the only therapeutic difference between the intramuscular injection of testosterone propionate and the oral administration of methyl test...

The Oral Administration of Methyl Testosterone in a Male Castrate

No AccessJournal of Urology1 Apr 1941The Oral Administration of Methyl Testosterone in a Male Castrate Rita S. Finkler, and George M. Cohn Rita S. FinklerRita S. Finkler More articles by this author , and George M. CohnGeorge M. Cohn More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(17)71083-XAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail © 1941 by The American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 45Issue 4April 1941Page: 548-551 Advertisement Copyright & Permissions© 1941 by The American Urological Association Education and Research, Inc.MetricsAuthor Information Rita S. Finkler More articles by this author George M. Cohn More articles by this author Expand All Advertisement PDF DownloadLoading ...