Optimal Healing Research Articles

COMPARATIVE CHARACTERISTICS OF CLINICAL INDICATORS OF THE CONDITION OF SCAR TISSUE OF THE FACIAL SKIN AT DIFFERENT STAGES OF THE POSTOPERATIVE PERIOD DEPENDING ON THE CHRONOTYPE OF THE PERSON.

The aim: The aim of the study was to improve preventive measures aimed at preventing the formation of pathological scarring of the skin in patients after elective surgery by intra- and postoperative use of cryopreserved placental cells. Materials and methods: The research was conducted in the Department of Maxillofacial Surgery on the basis of the Municipal Enterprise «Poltava Regional Clinical Hospital. M.V. Sklifosovsky» Poltava Regional Council. A total of 60 patients took part in the study, who were hospitalized for planned surgical interventions in the department of maxillofacial surgery. Results: We obtained clinical data on the processes of scar formation in patients with morning and evening chronotype on the background of local intraoperative injection of cryopreserved placenta extract into the wound. We have proved that short people with an established evening chronotype need additional preventive measures, especially on the 90th and 180th days of the postoperative period. Conclusions: From our study we can say with confidence that the drug "Cryocel" reduces epithelialization time, promotes optimal wound healing and leads to the formation of more aesthetic scars, which creates optimal both functional and cosmetic results.

Integrative Healthcare in India: A Paradigm Shift in Modern Medical Education and Practice of 21st Century

The famous Canadian physician William Osler once said that “the good physician treats the disease; the great physician treats the patient who has the disease.” Integrative health holds the same philosophy by bringing together conventional and complementary approaches in a coordinated care for the whole person (body, mind, spirit) rather than giving a specializing care to a particular organ system. Integrative health can be contrasted with alternative medicine in which a person takes nonconventional treatment instead of conventional medicine. According to the Consortium of Academic Health Centers for Integrative Medicine and Health, integrative medicine is defined as “the practice of medicine that reaffirms the importance of the relationship between practitioner and patient, focuses on the whole person, is supported by evidence, utilized all appropriate therapeutic and lifestyle approaches, healthcare professionals and disciplines to achieve optimal health and healing.

Is the future scarless? - Fibroblasts as targets for scarless wound healing: a narrative review.

Introduction: Scarless healing is the ideal outcome of wound healing and is exhibited in some species. This narrative review assembles the current understanding of fibroblast heterogenicity along with the latest fibroblast-related targets for scar reduction therapies. Human regenerative wound healing is deemed possible due to the wound regeneration already seen in the early gestation foetus. Methods: This literature narrative review was undertaken by searching PubMed and Web of Science databases and Google Scholar to find articles concerning the fibroblast involvement in wound healing. We evaluated and collated these articles to form a consensus of the current understanding of the field. Discussion: This article describes current understanding of fibroblast heterogenicity and involvement in wound healing, focusing on the role of fibroblasts during physiological scarring. We also present the current most promising targets involving fibroblasts in the reduction of scarring and how we can manipulate the behaviour of fibroblasts to mimic the wound regeneration models in the human foetus. These targets include the pro-fibrotic EN1 positive fibroblast lineage, TGFβ1 inhibition, and genetic therapies utilising miRNAs and siRNAs. Conclusion: No therapies are currently available to eradicate scarring; however, treatment options are available to reduce the appearance of scarring. Further research into the heterogenicity and interactions of fibroblasts in both the foetus and adult is needed, and this may lead to the development of novel treatments against scarring.Lay SummaryScarless healing refers to the repair of a wound with minimal residual scarring. The main cell responsible for the repair process is the fibroblast. It is now understood that there are different types of fibroblasts. Simply, some of these fibroblasts lead to scarring and some lead to regeneration. The early human foetus has mainly regenerative fibroblasts, but during aging the number of scarring fibroblasts increase to become the majority in the adult . Understanding how we can modify this process may ultimately result in the reduction in scarring. Currently, scar reduction therapies are aimed at optimal wound healing, surgical removal of abnormal scars, and using steroids and other drugs to encourage better wound repair by limiting the effect of scarring fibroblasts. Future therapies aim to target specific groups of fibroblasts to encourage regenerative wound healing. This narrative review aims to cover the current understanding of the different groups of fibroblasts and their effect on wound healing. We also cover the current and potential therapies that can be used to reduce scarring and suggest further areas for research in this field.

Interceptive treatment of the aberrant labial frenulum in the transition dentition stage using 808nm diode laser

The aberrant labial frenulum is one of the etiological factors for midline diastema. Such frenulum needs timely detection and early intervention in transition dentition to intercept the developing midline diastema in growing children. A conservative and minimal-invasive approach should be considered for managing the abnormal frenulum in the pediatric age group. This article presents a clinical case report on the successful and effective treatment of aberrant maxillary labial frenulum in a 10-year-old pediatric patient using the diode laser of 808 nm wavelength without infiltrative local anesthesia and sutures. The follow-up evaluation demonstrated optimum healing without any complications. The patient demonstrated cooperation and satisfaction with the laser surgery and its excellent healing outcomes.

Lumbar Disc Injury: Mechanisms and Intervention Options

Low back pain is prevalent in sport and the lumbar disc is a pain generator. Conservative care of these physically demanding patients is not consistent as outcome data has not established optimal strategies. Investigating disc injury mechanisms using experimental models will contribute to the creation of treatment strategies that will take into effect movements to avoid and minimize to reduce unwanted strain on the injured tissues throughout the recovery process. Additionally, how disc tissue acquires nutrition would help provide insight into activities that may facilitate the ability of disc tissue to sustain health and promote optimal healing. Rotational forces and compression in trunk-flexed positions may inflict injury to the annulus and endplate structures. Activity to facilitate nutrients through the endplate into the nucleus of the disc and restriction of exasperating movements may be effective in returning athletes to their desired levels of performance efficiently. The purpose of this review is to inquire possible disc injury mechanisms and how they occur in sport. It will also address recommendations for treatment interventions based on disc healing and metabolism evidence.

Presence of heavy metals of some selected neutraceutical products and their quality control assessment

Neutraceutical is combination of two words nutrition and pharmaceutical. The right amount of Neutraceutical is taken for the optimal healing and with fewer side effects. A Neutraceutical is a substance can be used in necessity of vitamins required by the body in order to full fill the demand of food which can be a curative as well as therapeutically used. This research was conducted to check the percentage of trace elements of zinc, copper iron, and lead, in the seven Neutraceutical products by the help of atomic absorption spectrum which are frequently used by the public of Quetta city i-e capital of Province of Baluchistan. The Physio-chemical properties like thickness, diameter, weight variation, dissolution, friability, are also checked and compared to the specification given in USP and BP pharmacopeia to find out that during manufacturing SOPs are followed or not. The seven empty bottles were selected to make solution of Neutraceutical in different products we washed every tablet with methanol in order to protect these bottles from germs, dust or any other contamination. The results obtained by quality control tests indicate that there was much deviation in the weight variation and hardness among these tablets of same batch but no deviation between thickness and diameter of these tablets. The result obtained by atomic absorption showed that percentage of these trace elements was more or less than the recommended limits when compared to specification given in USP and BP pharmacopeia. The concentration of these elements has much concern with the therapeutic efficacy of the Neutraceutical. The high concentration of lead is very much toxic to the lungs, liver and kidney. As well as the high concentration of iron, copper, zinc also affect the normal physiology of human being. It was concluded from this research that SOPs were not followed during manufacturing. So manufacturer should take consideration in respect to these formulations, SOPs should be followed as well as pre formulation studies and quality control tests should performed during all development procedures.

THE STUDY OF ANTIOXIDANTS TO EVALUATE THE HEALING EFFECTS OF PUNICA GRANATUM PEEL ON ACETIC ACID-INDUCED COLITIS IN CHARLES-FOSTER ALBINO RATS

Objective: The study aims to analyze the effect of 50% ethanolic extract of the dried Punica granatum peel (PGE) on the healing of acetic- acid-induced colitis in rats. Methods: Colitis was induced in rats using 50% acetic acid and then PGE extract was administered by oral route daily for 14 days to those rats. Optimal healing was observed by the administration of a 100 mg/kg dose of PGE extract. Effectiveness of the above-mentioned dosage of PGE, biochemical parameters namely- antioxidants-superoxide dismutase and reduced glutathione were studied on 18 h fasting rats on the 15th day of the experiment. Results: The results were suggestive of the healing properties of PGE extract by reduction of the inflammation and mucosal damage in the colon of those rats. The effect was established by the levels of antioxidants that indicate healing of the mucosal damage. The safety of extract was established by the effective administration of 10 times the therapeutic dose, that is, 1000 mg/kg dosage of the PGE extract with no noticeable adverse effects or side effects related to autonomic nervous system or central nervous system. Conclusion: PGE extract was found to be effective in healing mucosal damage due to colitis by controlling the infection and reducing the inflammation.

THE STUDY OF FREE RADICALS TO EVALUATE THE HEALING EFFECTS OF PUNICA GRANATUM PEEL ON ACETIC ACID-INDUCED COLITIS IN RATS

Objective: The objective of the study was to analyze the effect of 50% ethanolic extract of the dried Punica granatum peel (PGE) on the healing of acetic acid-induced colitis in rats. Methods: Colitis was induced in rats using 50% acetic acid and then PGE extract was administered by oral route daily for 14 days to those rats. Optimal healing was observed by the administration of a 100 mg/kg dose of PGE extract. Effectiveness of the above-mentioned dosage of PGE on biochemical parameters, basically free radicals – nitric oxide and lipid peroxidation was studied on 18 hours fasting rats on the 15th day of the experiment. Results: The results were suggestive of the healing properties of PGE extract by reduction of the inflammation and mucosal damage in the colon of those rats. The healing effects were established by the estimation and study of free radicals taken from the mucosal samples of the rat’s colon. The safety of extract was established by the effective administration of 10 times the therapeutic dose, that is, 1000 mg/kg dosage of the PGE extract with no noticeable adverse effects or side effects related to ANS or central nervous system. Conclusion: PGE extract was found to be effective in healing mucosal damage due to colitis by controlling the infection and reducing the inflammation.

BCL-Xl Driven Accumulation of Dysfunctional Mitochondria in Aged Stromal Cells Impairs the Haematopoietic Stem Cell Response to Stress

The burden of infections is known to increase with age. Not only is ageing associated with greater susceptibility to infections but also an increase in subsequent morbidity and mortality. The bone marrow (BM) niche is essential for the body's response to infection. Haematopoietic stem and progenitor cells (HSPCs) heavily rely on their supporting BM microenvironment to effectively expand and differentiate in response to stress and infection (1). The role of senescent cells has been explored in a number of age-related diseases including acute myeloid leukaemia (2). Here we explore the role of senescence during natural ageing in the BM microenvironment, the mechanism which drives this and how this impacts on the metabolic health of HSPCs.BM was isolated from aged (18-24 months) and young (8-12 weeks) C57Bl/6 mice and flow cytometry was used to compare mitochondrial membrane potential (ΔΨm) and mitochondrial ROS in HSPCs. Results show that HSPCs from aged animals increase in numbers and accumulate mitochondria with low membrane potential with lower mitochondrial ROS. Next, young and aged mice were treated with lipopolycaccharide (LPS). Metabolic analysis revealed that HSPCs from young mice increase metabolism of mitochondrial TCA cycle substrates in response to LPS whereas aged HSPCs continued to rely on glycolysis. When HSPCs from aged C57Bl/6 mice (CD45.2+) were FACS purified and adoptively transferred into young PepCboy (CD45.1+) mice, thus removing them from the aged BM microenvironment, they were able to recover their mitochondrial health and showed an improved metabolic response to treatment with LPS. Furthermore, qRT-PCR analysis of p16 and p21 expression in HSPCs and mesenchymal stromal cells (MSC) showed that MSCs, but not HSPCs, acquire a senescent phenotype in aged mice, and depletion of senescent cells in the p16-3MR mouse model (3) allowed recovery of HSPC mitochondrial function and response to LPS. Mechanistically, we found a significant upregulation of the anti-apoptotic protein BCL-XL in MSCs of aged mice. This has previously been described to drive the senescent phenotype and prevent apoptosis in senescent cells. By over-expressing GFP-tagged BCL-XL in MSCs and then co-culturing them with LSKs we were able to show that BCL-XL is transferred from MSCs to HSPCs in vitro. Finally, we demonstrated that targeting BCL-XL in vivo, using the senolytic drug ABT-263, in aged mice can restore the HSPC metabolic response to stress resulting in upregulation of TCA cycle metabolism.In conclusion, we show that the aged BM microenvironment is responsible for the HSPC metabolic shortfall resulting in impaired response to stress. Targeting the senescent cells in the environment restored the HSPC metabolic function and their response to infection in aged mice. This suggests that manipulation of the ageing BM microenvironment can help to improve the body's response to infection.1. Mistry JJ, Marlein CR, Moore JA, Hellmich C, Wojtowicz EE, Smith JGW, et al. ROS-mediated PI3K activation drives mitochondrial transfer from stromal cells to hematopoietic stem cells in response to infection. Proc Natl Acad Sci U S A. 2019;116(49):24610-9.2. Abdul-Aziz AM, Sun Y, Hellmich C, Marlein CR, Mistry J, Forde E, et al. Acute myeloid leukemia induces protumoral p16INK4a-driven senescence in the bone marrow microenvironment. Blood. 2019;133(5):446-56.3. Demaria M, Ohtani N, Youssef SA, Rodier F, Toussaint W, Mitchell JR, et al. An essential role for senescent cells in optimal wound healing through secretion of PDGF-AA. Dev Cell. 2014;31(6):722-33. DisclosuresNo relevant conflicts of interest to declare.

Undressing the choice of dressings.

There is a vast number of wound dressings to choose from, which can make selecting the most suitable dressing for a wound a daunting prospect. This article highlights how the wound can guide the doctor's decision. Although all wound dressings are intended to achieve optimal wound healing and protection from contamination, they do so to varying degrees depending on the qualities of the dressing and the wound itself. This article looks at a range of dressings, from traditional to newer dressings, and evaluates their benefits and contraindications to help determine where they are best suited for use in wound management.

Regional Anesthesia Use in Pediatric Burn Surgery: A Descriptive Retrospective Series.

Pediatric burns are a common and often devastating injury. Treatment, reconstruction, and rehabilitation are painful experiences. For some, the experience triggers post-traumatic stress disorder and/or a chronic pain syndrome. Given the role pain plays as a major secondary disease, it must be addressed to achieve optimal healing. Regional anesthesia has been used extensively to manage postoperative pain and reduce the need for opioids following other surgical procedures in children. However, regional anesthesia is not widely used in pediatric burn care. We present a descriptive, retrospective case series of 15 pediatric burn patients who received regional anesthesia as part of their intra-operative pain management. In our patient population, we saw low levels of anesthetic as well as opioid usage with well-controlled pain. In this cohort, 93% of patients scored a 0/10 on the Face, Legs, Activity, Cry and Consolability (FLACC) scale for pain by post-anesthesia care unit (PACU) discharge, with an average PACU stay of 70 minutes. Thirty-three percent of patients received no opioids, with the average opioid dose being 0.06mg/kg morphine equivalents. This case series serves to make clinicians aware of the feasibility of use and benefits of regional anesthesia in pediatric burn patients requiring operative repair.

Advances in Nanotechnology towards Development of Silver Nanoparticle-Based Wound-Healing Agents.

Since antiquity, silver-based therapies have been used in wound healing, wound care and management of infections to provide adequate healing. These therapies are associated with certain limitations, such as toxicity, skin discolouration and bacterial resistance, which have limited their use. As a result, new and innovative wound therapies, or strategies to improve the existing therapies, are sought after. Silver nanoparticles (AgNPs) have shown the potential to circumvent the limitations associated with conventional silver-based therapies as described above. AgNPs are effective against a broad spectrum of microorganisms and are less toxic, effective at lower concentrations and produce no skin discolouration. Furthermore, AgNPs can be decorated or coupled with other healing-promoting materials to provide optimum healing. This review details the history and impact of silver-based therapies leading up to AgNPs and AgNP-based nanoformulations in wound healing. It also highlights the properties of AgNPs that aid in wound healing and that make them superior to conventional silver-based wound treatment therapies.

Low defect and high electrical conductivity of graphene through plasma graphene healing treatment monitored with in situ optical emission spectroscopy

Fundamental studies on graphene (Gr) and its real device applications have been affected by unavoidable defects and impurities which are usually present in synthesized Gr. Therefore, post treatment methods on Gr have been an important subject of research followed by the community. Here, we demonstrate a post-treatment of cm-sized CVD-grown graphene in a Radio Frequency-generated low-pressure plasma of methane and hydrogen to remove oxygen functional groups and heal the structural defects. The optimum plasma treatment parameters, such as pressure, plasma power, and the ratio of the gases, are optimized using in-situ optical emission spectroscopy. This way we present an optimal healing condition monitored with in situ OES. A twofold increase in the conductivity of plasma-treated Gr samples was obtained. Plasma treatment conditions give insights into the possible underlying mechanisms, and the method presents an effective way to obtain improved Gr quality.

Roles of Non-Canonical Wnt Signalling Pathways in Bone Biology.

The Wnt signalling pathway is one of the central signalling pathways in bone development, homeostasis and regulation of bone mineral density. It consists of numerous Wnt ligands, receptors and co-receptors, which ensure tight spatiotemporal regulation of Wnt signalling pathway activity and thus tight regulation of bone tissue homeostasis. This enables maintenance of optimal mineral density, tissue healing and adaptation to changes in bone loading. While the role of the canonical/β-catenin Wnt signalling pathway in bone homeostasis is relatively well researched, Wnt ligands can also activate several non-canonical, β-catenin independent signalling pathways with important effects on bone tissue. In this review, we will provide a thorough overview of the current knowledge on different non-canonical Wnt signalling pathways involved in bone biology, focusing especially on the pathways that affect bone cell differentiation, maturation and function, processes involved in bone tissue structure regulation. We will describe the role of the two most known non-canonical pathways (Wnt/planar cell polarity pathways and Wnt/Ca2+ pathway), as well as other signalling pathways with a strong role in bone biology that communicate with the Wnt signalling pathway through non-canonical Wnt signalling. Our goal is to bring additional attention to these still not well researched but important pathways in the regulation of bone biology in the hope of prompting additional research in the area of non-canonical Wnt signalling pathways.

Achieving effective patient outcomes with PolyMem® Silicone Border.

Clinicians are under increasing pressure to provide high-quality patient outcomes at a reduced cost. Increasingly, community staff must acquire knowledge on advanced wound care products to cope with the growing caseload demands. This article describes the use of PolyMem® dressings to reduce pain, inflammation, oedema and bruising and their ability to debride and absorb exudate while providing an optimum healing environment. The PolyMem range includes multifunctional dressings for various painful chronic wounds. This article also presents five case studies with particularly good patient outcomes where PolyMem dressings were the primary dressing. All five patients were holistically assessed to enable consistent evidence-based treatment decisions. In four cases, the new PolyMem Silicone Border dressing was used. The patients found the PolyMem Silicone Border dressing comfortable and gentle on removal even when the skin was extremely fragile. The right dressing used at the right time on the right patient can improve patient outcomes.

Comparison of neointimal healing with bioresorbable scaffolds and drug-eluting stents in patients with stable ischaemic heart disease

Aim. To examine the process of neointimal formation after bioresorbable scaffolds (BRS) implantation using optical coherence tomography (OCT) in patients with stable coronary artery disease (SCAD) and to determine relationship between neointimal healing and biochemical parameters of inflammation.Methods. Patients with SCAD (n = 20) who were indicated for percutaneous coronary intervention (PCI) were enrolled. Patients were randomised into two groups as per the stent type. The treatment group comprised 10 patients who were implanted with BRS ABSORB (Abbott Laboratories, Abbott Park, USA) during PCI. The comparison group comprised 10 patients who were implanted with DES XIENCE (Abbott Laboratories, Abbott Park, USA) during PCI. All the patients underwent OCT imaging during PCI. Subsequently, 18 patients were subjected to coronary angiography with OCT imaging in 12 mon. The primary endpoint was the 12-month neointimal healing (NIH) score. Secondary endpoints were clinical outcomes (all-cause hospitalisation, myocardial infarction, probable stent thrombosis and death), OCT parameters at the 12-month follow-up and biochemical markers dynamics.Results. Initial angiographic data analysis indicated a higher rate of balloon pre-dilatation (100% vs. 30%; р = 0,003) and post-dilatation (100% vs. 20% р = 0,001) in patients of the treatment group. According to OCT, the NIH score was significantly higher in the XIENCE group [0 versus 9,14 (1,63–17,55); р = 0,008] at 12 mon. There was no significant difference in the clinical outcomes between the two groups. However, the ABSORB group had an increased CD40 level after 4–5 d of PCI. In agreement with the results of correlation analysis, there was an inverse correlation between the NIH score and CD40 level at 4–5 d after PCI (r = −0,576; р = 0,016). The cut-off value of CD40 level at 4–5 d after PCI was 47,5 ng/mL for the detection of optimal neointimal healing.Conclusion. In patients with SCAD, BRS demonstrated higher rate of neointimal healing than everolimus-coated stents. There was a registered inverse correlation of the NIH score with the CD40 level at 4–5 days after PCI. CD40 level > 47,5 ng/mL at 4–5 d after PCI increases the likelihood of optimal neointimal healing as per OCT data.Received 19 February 2021. Revised 7 June 2021. Accepted 16 June 2021.Funding: The study did not have sponsorship.Conflict of interest: The authors declare no conflicts of interests.Contribution of the authorsConception and study design: I.S. Bessonov, N.A. Musikhina, T.I. PetelinaData collection and analysis: S.S. Sapoznikov, I.S. Bessonov, N.А. Galeeva, A.O. DyakovaStatistical analysis: S.S. Sapoznikov, E.A. GorbatenkoDrafting the article: S.S. Sapoznikov, N.А. Galeeva, A.O. DyakovaCritical revision of the article: I.S. Bessonov, S.S. Sapoznikov, E.A. Gorbatenko, N.A. MusikhinaFinal approval of the version to be published: S.S. Sapoznikov, N.А. Galeeva, I.S. Bessonov, N.A. Musikhina, T.I. Petelina, A.O. Dyakova, E.A. Gorbatenko

The Role of Social Mechanisms in Achieving the Optimal Healing Environment for Oncology Patients Rooms (Adolescents and Young Adults Category-AYA) الاليات الاجتماعية ودورها فى تحقيق البيئة الاستشفائية المثلى لغرف مرضى الأورام فئة المراهقين وصغار البالغين

This paper discusses the importance of social mechanisms as one of key elements for achieving optimal healing environment-OHE for oncology patients' rooms, by overviewing the current status of some oncology centers, which focus solely on providing medical treatment without taking into account social, psychological, emotional and spiritual needs of patients, which in turn negatively affect the treatment and recovery of patients, especially adolescents and young adults–AYA, where the challenges of emotional and social resulting from cancer with challenges resulting from adolescence and adulthood. This paper aims to extract social mechanisms for oncology patient rooms, and indicate their role and priorities which will be applied with matching needs of patients of AYA category, mitigating their disorders, and impacts on patients' health outcomes. This paper attempts to reach the best appropriate model to achieve these mechanisms, by analyzing the current situation of selected samples from case study of the South Egypt Cancer Institute in Asyut. Through this analysis, this paper presents a set of results and recommendations that should be followed to develop patient rooms, which take into account the provision of social mechanisms and the implementing priorities depending on impact level of each element in order to reach the suitable setting for the optimal healing environment of the room. This paper relies the analytical descriptive approach to deal with the research problem by revising literature that dealt with the characterization and analyzing the needs of AYA oncologists in patient rooms, identifying the disorders and effects of the disease on this category, then identifying the appropriate social mechanisms that can be match their needs in the rooms. An analytical and deductive approach is followed through implementing various research tools and procedures to analyze the coverage of current status to know how these mechanisms are achieved within different models of patient rooms and how they affect patient health, then to analyze data and outcomes results. Finally, this paper concludes with a comprehensive chart of social mechanisms’ matrix suitable for optimal healing environment in AYA patients’ rooms. تناقش الورقة البحثية أهمية الآليات الاجتماعية کأحد العناصر الأساسية لتحقيق منظومة البيئة الاستشفائية المثلى لغرف مرضى الأورام، فمن خلال دراسة واقع بعض مراکز علاج الأورام تبين أنها ترکز على تقديم العلاج الطبي فقط دون الأخذ في الاعتبار تلبية الاحتياجات الاجتماعية والنفسية والعاطفية للمرضى بما يؤثر سلبا على علاج المرضى وسرعة الشفاء خاصة فئة المراهقين وصغار البالغين، حيث تتداخل التحديات العاطفية والاجتماعية الناتجة عن المرض مع تحديات مرحلة المراهقة والبلوغ. تهدف الورقة البحثية إلى استخلاص الآليات الاجتماعية (کأحد آليات تحقيق البيئة الاستشفائية المثلى) لغرف مرضى الأورام، وبيان دورها وأولويات تطبيقها في تلبية احتياجات المرضى فئة المراهقين وصغار البالغين، والتخفيف من اضطراباتهم، وتقييم الوضع الراهن للبيئة المبنية لمعرفة مدى تحقيق هذه الآليات. تحاول الورقة البحثية تحديد أفضل نماذج الغرف الملائمة لتحقيق هذه الآليات من خلال تحليل نماذج مختارة من معهد جنوب مصر للأورام بمدينة أسيوط، ومن خلال هذه التحليلات تعرض مجموعة من النتائج والتوصيات اللازمة لتطوير غرف المرضى بحيث تراعي توفير الآليات الاجتماعية وأولويات تطبيقها تبعا لدرجة تأثير کل منها وصولا إلى استيفاء متطلبات الوضع الأمثل للبيئة الاستشفائية للغرفة. تعتمد الورقة البحثية على المنهج الوصفي التحليلي للتعامل مع المشکلة البحثية من خلال دراسة الأدبيات التي تناولت توصيف وتحليل احتياجات مرضى الأورام فئة المراهقين وصغار البالغين، والتعرف على اضطرابات وتأثيرات المرض على هذه الفئة، ومن ثم تحديد الآليات الاجتماعية الملائمة، تبعه المنهج التحليلي الاستنباطي من خلال استخدام الأدوات والإجراءات البحثية المختلفة لتحليل الوضع الراهن ومعرفة مدى تحقق هذه الآليات داخل البيئة المبنية لنماذج غرف المرضى، ومن ثم تحليل ما تم التوصل إليه من بيانات ونتائج. تخلص الورقة البحثية إلى وضع تصور شامل لمصفوفة الآليات الاجتماعية الملائمة للمرضى فئة المراهقين وصغار البالغين.

How to set up for dental extractions

Exodontics is the branch of dental surgery concerned with the extraction of teeth. Dental extraction involves the removal of teeth from the dental alveolus (socket) in the alveolar bone of the incisive bones, maxilla and mandibles. There are two types of extractions the veterinary surgeon (VS) can perform — closed or open — and both are associated with tissue disruption and manipulation to varying degrees, which will inevitably initiate an inflammatory and pain response, which can prolong healing. The role of the veterinary nurse (VN) in preparing equipment and consumables for extraction should not be underestimated; excellent preparation can reduce surgical time, reduce the length of time the patient is anaesthetised, and ensure high-quality extractions can be performed by the VS to promote optimal postoperative healing.

MP47-12 IMPACT OF URETHRAL GROWTH FACTORS ON IN VITRO UROTHELIAL GROWTH

You have accessJournal of UrologyPediatric Urology III (MP47)1 Sep 2021MP47-12 IMPACT OF URETHRAL GROWTH FACTORS ON IN VITRO UROTHELIAL GROWTH Christopher Foster, and Courtney K. Rowe Christopher FosterChristopher Foster More articles by this author , and Courtney K. RoweCourtney K. Rowe More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000002068.12AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Urethral injuries and congenital anomalies that require surgery are often associated with poor healing that result in strictures and scarring. Current regenerative medicine techniques focus on scaffolds and growth factors; however, there is very limited preliminary research showing which growth factors are ideal for urothelial healing. The goal of this study was to 1) develop an in vitro model of urethral healing and 2) evaluate differences in healing rates between growth factors that are associated with the urethra to establish a foundation upon which future regenerative medicine can expand. We hypothesize that one of six candidate growth factors which have been found present during urethral healing would provide optimal urethral healing in vitro. METHODS: Human urothelial epithelial cells from a bladder source were purchased. Cells were seeded onto prefabricated wound healing dishes and allowed to reach near confluence. Four hours prior to the start of the assay, cells were starved with growth factor free media. At time 0, the well insert was removed and fresh media with one of six candidate growth factors was added to respective dish. At time 0, 6, 10, 14, 24, and 48 hours microscopic images were taken of the wound gap. Images were analyzed using Image-J and % wound gap coverage was considered healing percent of the wound. Comparative statistics were performed using ANOVA with tukey multiple comparisons test where p<0.0002. RESULTS: Insulin-like growth factor 1 (IGF-1) at concentrations of 10 and 100 ng/ml had a statistically significant increase in percentage healing compared to both the controls (Figure A). IGF-1 at 100 ng/ml was the only growth factor to show 100% healing at 48 hours. Epidermal growth factor (EGF) at concentrations of 1 and 10 ng/ml had a statistically significant increase in healing compared to the growth factor free media control (Figure A). FGF-basic, PDGF-BB, TGF-β1, and VEGF had no significant differences compared to the growth factor free media control. CONCLUSIONS: IGF-1 and EGF both drive healing in this urothelial epithelial growth model with IGF-1 having the greatest effect and providing 100% healing at 48 hours. Future studies should evaluate how these individual growth factors effect urothelial healing in an in vivo model. Source of Funding: None © 2021 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 206Issue Supplement 3September 2021Page: e827-e828 Advertisement Copyright & Permissions© 2021 by American Urological Association Education and Research, Inc.MetricsAuthor Information Christopher Foster More articles by this author Courtney K. Rowe More articles by this author Expand All Advertisement Loading ...

Research priorities for acute wounds in adults in Australia: a scoping review protocol

Background. Preservation and restoration of skin integrity following surgery is paramount for optimal patient wound healing outcomes. Acute wounds such as incisional wounds, skin tears, trauma or burn injury cause pain, reduce quality of life and are a considerable economic burden to the Australian healthcare system. Despite considerable advances in surgical technique and even with a panacea of innovative novel wound dressings, our scientific and clinical understanding of wound healing prevention and management of acute wound complications continue to present a considerable challenge to clinicians and policy makers. Understanding the gaps in knowledge and identifying clinical practice deficits are key for prevention and management of acute wounds. Aims. This scoping review aims to (i) map current research evidence and outcomes in acute wounds management, (ii) map current research evidence and outcomes in acute wounds prevention and (iii) determine research gaps in acute wound research relevant to Australia. Methods. The framework for this scoping review will utilise the PRISMA-ScR framework developed by Tricco et al.1. We will search the following databases – Medline, CINAHL, Embase, Joanna Briggs Institute (JBI), Cochrane Library and PubMed from January 2010 to March 2021. Trial registries (e.g. ISRCTN, ANZCTR and clinicaltrials.gov) and websites and publications of professional associations for wound care will also be searched. Two reviewers will independently screen all titles, abstracts and full text for articles to include. Conflicts will be solved by a third reviewer. This scoping review will include both qualitative and quantitative studies on acute wounds conducted in Australia. We will extract data from eligible articles and results will be grouped according to area of research and synthesised in a narrative review. Ethics and dissemination. As we will use data (i.e., journal articles) from publicly available platforms this scoping review does not require ethical approval. Findings will be disseminated through a peer-reviewed journal and conference presentation and social media platforms.