Optimal Storage Conditions Research Articles

Bioflocculant Producing Bacillus megaterium from Poultry Slaughterhouse Wastewater: Elucidation of Flocculation Efficacy and Mechanism

The study focused on isolating bioflocculant-producing microorganisms from poultry slaughterhouse wastewater (PSW). Microorganisms (n = 20) were isolated, and the D2 isolate, identified as Bacillus megaterium using 16S rDNA and RpoD (sigma 70), had maximum flocculation activity. Furthermore, characteristics of the bioflocculant produced by B. megaterium were determined, and the optimum storage conditions, including the flocculation mechanism, were identified. The bioflocculant was composed mainly of polysaccharides and proteins and was better stored frozen in a crude form. Furthermore, the flocculation efficacy was assessed using response surface methodology at pH 4 (min) and 9 (max), bioflocculant dosage of 1% (min) and 3% (max, v/v), indicating pH 6.5 and dosage of 2% (v/v) as optimum flocculation conditions for floc formation under ambient temperature. These results were further confirmed with microscopy assessments with zeta potential measurements confirming that the bioflocculant was ionic, albeit charge neutralization was not the primary mechanism for floc agglomeration. Hydrogen bonding was predominant, indicative of a neutralization-bridging mechanism, an assertion also based on the functional groups prevalent in the isolate-B. megaterium. The results obtained indicate that bioflocculants can be used to treat isolates that are sourced from wastewater.

The Optimal Storage Condition and Storage Time of Umbilical Cord Blood from Collection to Preparation

To explore the optimal storage condition and time of umbilical cord blood from collection to preparation. Collect cord blood samples from 30 healthy newborns, with each new born's umbilical cord blood was divided into two parts on average. One part was stored in cold storage (4 ℃) and the other was stored at room temperature (20-24 ℃). Samples were taken at 24, 36, 48, 60 and 72 h, respectively, total nucleated cells (TNC) count and TNC viability was analyzed. Flow cytometry was used to detect the ratio of viable CD34+ cells to viable CD45+ cells and viability of CD34+ cells, and colony-forming unit-granulocyte-macrophage (CFU-GM) count was performed by hematopoietic progenitor cell colony culture. The change trend of each index over time was observed, and the differences in each index was compared between cold storage and room temperature storage under the same storage time. The TNC count (r 4 ℃=-0.9588， r 20-24 ℃=-0.9790), TNC viability (r 4 ℃=-0.9941， r 20-24 ℃=-0.9970), CD34+ cells viability (r 4 ℃=-0.9932， r 20-24 ℃=-0.9828) of cord blood stored in cold storage (4 ℃) and room temperature storage (20-24 ℃) showed a consistent downward trend with the prolongation of storage time. The percentage of viable CD34+ cells (r 4 ℃=0.9169， r 20-24 ℃=0.7470) and CFU-GM count (r 4 ℃=-0.2537， r 20-24 ℃=-0.8098) did not show consistent trends. When the storage time was the same, the TNC count, TNC viability, CD34+ cells viability and CFU-GM count of cord blood stored in cold storage were higher than those stored at room temperature. Under the same storage time (24, 36, 48, 60 or 72 h), TNC viability in room temperature storage was significantly lower than that in cold storage (P <0.001), but TNC count, percentage of viable CD34+ cells and CFU-GM count were not significantly different between room temperature storage and cold storage. When stored at room temperature for 24 h and 36 h, the viability of CD34+ cells was significantly lower than that in cold storage (P <0.001, P <0.01), when the storage time for 48, 60 and 72 h, there was no significant difference in the CD34+ cells viability between room temperature storage and cold storage. It is recommended that cord blood be stored in cold storage (4 ℃) from collection to preparation, and processed as soon as possible.

Influence of temperature, time, and moisture content on rheology of tomatoes and pepper purees

This study explored how temperature, time, and moisture content affect the rheological properties (apparent viscosity, flow behavior index, and consistency coefficient) of stored tomato and pepper purees. These purees were prepared with moisture contents of 85%, 90%, and 95% (w/v) using the hot-break method and tested over 6 days at 2-day intervals and temperatures of 5°C, 10°C, and 15°C. Results displayed distinct ranges for apparent viscosity, consistency coefficient, and flow behavior indices: tomato puree (2,519.9-4,324.6 mPa · s, 258.0-550.6 mPa · Sn, 1.80-0.48) and pepper puree (2,105.6-4,562.0 mPa · s, 268.4-580.4 mPa · Sn, 0.22-0.48). The temperature and storage time had significant (p≤0.05) effects, but moisture content did not affect these properties. Flow behavior and consistency coefficients demonstrated relative variation with apparent viscosity, indicating pseudoplastic behavior. Optimal processing and storage conditions were identified within specific ranges: 13.21-14.42°C for 2 days with 92.22-94.23% (w/v) moisture content for pepper, and 8.42-11.77°C for 2-6 days with 85% (w/v) moisture for tomato.

(315) - Evaluation of Ventilation at 10°C as the Optimal Storage Condition for Donor Lungs in a Murine Transplant Model

(315) - Evaluation of Ventilation at 10°C as the Optimal Storage Condition for Donor Lungs in a Murine Transplant Model

Improved Long-Term Preservation of Cannabis Inflorescence by Utilizing Integrated Pre-Harvest Hexanoic Acid Treatment and Optimal Post-Harvest Storage Conditions.

The effort to maintain cannabinoid and terpene levels in harvested medicinal cannabis inflorescence is crucial, as many studies demonstrated a significant concentration decrease in these compounds during the drying, curing, and storage steps. These stages are critical for the preparation and preservation of medicinal cannabis for end-use, and any decline in cannabinoid and terpene content could potentially reduce the therapeutic efficacy of the product. Consequently, in the present study, we determined the efficacy of pre-harvest hexanoic acid treatment alongside four months of post-harvest vacuum storage in prolonging the shelf life of high THCA cannabis inflorescence. Our findings indicate that hexanoic acid treatment led to elevated concentrations of certain cannabinoids and terpenes on the day of harvest and subsequent to the drying and curing processes. Furthermore, the combination of hexanoic acid treatment and vacuum storage yielded the longest shelf life and the highest cannabinoid and mono-terpene content as compared to all other groups studied. Specifically, the major cannabinoid's-(-)-Δ9-trans-tetrahydrocannabinolic acid (THCA)-concentration decreased by 4-23% during the four months of storage with the lowest reduction observed following hexanoic acid pre-harvest treatment and post-harvest vacuum storage. Hexanoic acid spray application displayed a more pronounced impact on mono-terpene preservation than storage under vacuum without hexanoic acid treatment. Conversely, sesqui-terpenes were observed to be less prone to degradation than mono-terpenes over an extended storage duration. In summation, appropriate pre-harvest treatment coupled with optimized storage conditions can significantly extend the shelf life of cannabis inflorescence and preserve high active compound concentration over an extended time period.

Dual Effects of Cold Storage and Stored Host Eggs of Spodoptera frugiperda (Smith) (Lepidoptera: Noctuidae) on the Reproductive Capacity of Telenomus remus Nixon (Hymenoptera: Scelionidae).

Spodoptera frugiperda is the preferred host of the parasitoid Telenomus remus. Cold storage can preserve a sufficient quantity of parasitoids and their hosts in a laboratory colony for mass release. First, the effects of cold storage on the reproductive capacity of T. remus reared on non-stored S. frugiperda eggs and the hatching rate of unparasitized S. frugiperda eggs were investigated. Further, the dual effects of cold storage and stored S. frugiperda eggs on the reproductive capacity of T. remus were studied to determine the optimal storage conditions and the maximal shelf life for both the host and the parasitoid. The emergence rate, the number of adults produced, and the female proportion of T. remus were affected by cold storage factors. Pupae stored at 13 °C for 15 days is optimum for T. remus reared on non-stored S. frugiperda eggs. Spodoptera frugiperda eggs could only be stored at 10 °C for five days to be suitable for rearing T. remus. The optimum cold storage conditions for T. remus parasitizing stored eggs were 7 °C for 5 days in the larval stage. The maximal shelf lives of T. remus parasitizing cold-stored S. frugiperda eggs were 10 days. Cold storage affected the hatching rate of S. frugiperda eggs, thereby further affecting the reproductive capacity of T. remus. The findings suggest that different storage conditions should be used when mass-rearing T. remus on stored and non-stored eggs. Telenomus remus should be reproduced using fresh laid S. frugiperda eggs for maximum shelf life.

Effect of storage conditions on nutritional value, oil content, and oil composition of sesame seeds

The increase in global demand consumption of sesame seeds as a nutritious food source and the limited areas suitable for cultivation have made the world face the challenge of determining the optimal storage conditions for sesame seeds. In this study, sesame seeds were stored in three different temperature and humidity conditions: High Temperature (HT: 60 °C, 100% humidity), Room Temperature (RT: 20 °C, 70% humidity), and Refrigerator Temperature (FT: 4 °C, 30% humidity), compared to the control (20 °C, 3% humidity). The soluble sugar, vitamins and protein contents of the sesame seeds were determined following standard methods. Fatty acids were determined by gas chromatography-mass spectrometry (GC-MS). Oil yield 11.2% increased under the HT storage condition, but no significant change was observed under the other storage conditions. HT conditions caused the degradation of fatty acids, while RT and FT conditions maintained the structure of fatty acids. Vitamin C levels decreased in FT, RT and HT storage conditions 0.0622, 0.0496, 0.0118 mg/100 g and vitamin B12 levels decreased 11.73, 12.02, 10.79 μM/100 g, respectively. Vitamin B9 levels increased in FT, RT and HT storage conditions 299.3, 222.3, 216.8 μg/100 g, respectively and vitamin E decreased 135.3, 30.7 mg/100 g under HT and FT conditions compared to control, however, no changes were observed under RT conditions. Total soluble sugar content increased 157.7 mg/100 g in FT conditions while it decreased 73.56 mg/100 g in HT conditions. This study showed storage conditions affected the nutritional value and phytochemical properties of sesame seeds. High temperature and humidity adversely affect the oil quality, fatty acid structure, and vitamin and sugar content of sesame seeds. Sesame intake in human dietary affected by the cooking process, such as roasting in dressing bread and confectionery reduce fatty acids, vitamins, and sugars in seeds. It seems that RT and FT are the optimal conditions for preserving fatty acids with high nutritional value in sesame seeds, although the moisture is different. The findings are important in controlling and maintaining the nutritional value of sesame during storage.

Development and Assessment of Hydroxypropyl Methylcellulose-Based Floating Tablets for Ciprofloxacin HCL Using Direct Compression Technique

The key findings and conclusions include: such as formulation approaches as aim and objective Development of floating tablets using sodium bicarbonate and HPMC aimed at enhancing gastric residence time for improved drug bioavailability. Physicochemical Compliances The formulated tablets met compliance standards for various physicochemical parameters, including dimensions, floating time, tablet density, and drug content. Method of Formulations F2, F5, and F6 displayed favorable drug release profiles, with the F7 formulation exhibiting excellent release characteristics. in the evaluation the drug release kinetics studies show Kinetic analysis revealed that F2, F5, F6, and F7 formulations followed the Korsmeyer–Peppas model, indicating non-Fickian diffusion with 'n' values ranging from 0.521 to 0.633 and the stability indicates the Optimal storage conditions for stability were determined as 2-8°C for 60 days. Formulations F2, F5, F6, and F7 demonstrated stability at room temperature, 40°C, and 2-8°C for 30 days, with refrigerated storage maintaining stability throughout the 60 days. In conclusion, the developed hydrodynamically balanced tablets of Ciprofloxacin HCl exhibit promising physicochemical characteristics, dissolution profiles, and stability. These tablets hold the potential for enhancing drug bioavailability, making them a viable option for localized drug delivery in the upper gastrointestinal tract.

Enhanced Preservation of Bioactives in Wild Garlic (Allium ursinum L.) through Advanced Primary Processing

Medicinal and aromatic plants, such as wild garlic (Allium ursinum), are valuable sources of bioactive compounds in traditional and modern medicine. However, the challenges in cultivating wild garlic and its seasonality limit its availability primarily to early spring. To maintain the quality of wild garlic, effective postharvest practices are essential, considering the delicate nature of its leaves and their susceptibility to rapid decay. This study focuses on implementing eco-friendly postharvest practices to address the seasonality and perishability of wild garlic. Optimizing storage conditions and drying processes is essential for extending its shelf life and preserving bioactive components. Two postharvest approaches were evaluated, with vacuum packaging at 4 °C demonstrating the most effective preservation of sensory attributes and bioactive composition over a 9-day period. Additionally, different drying methods, including convective and vacuum drying at various temperatures, were investigated. When the best drying temperature was selected, the results revealed that convective drying is more efficient at preserving phenolic compounds in wild garlic leaves at 60 °C. Conversely, vacuum drying shows superior preservation of alliinase activity and total thiosulfinate content at 70 °C. Furthermore, this study explores the utilization of unused wild garlic, which is typically discarded as waste during storage. By employing suitable drying methods, this material can be preserved and provide a valuable source of phenolic compounds (7.17–10.12 g GAE/100 g) and thiosulfinate (9.35–12.72 mg AC/110 g) compounds for various extraction processes. Accordingly, this research presents significant implications for the integration of wild garlic into diverse industries, particularly in the fields of food and pharmaceutical sectors. The findings offer opportunities for sustainable utilization, economic benefits, and a more environmentally friendly and economically viable approach to wild garlic production and processing.

Investigation of select radionuclides stability in urine under various conditions for liquid scintillation counting (LSC).

Liquid Scintillation Counting (LSC) gross alpha/beta screening is a valuable tool for providing rapid laboratory response for the analysis of human clinical urine samples during a large-scale radiation incident event. Verification of method performance, as required for clinical laboratory testing, is accomplished by the evaluation of routine, periodic measurements of radioactive spiked samples for quality control, performance testing, and accuracy checks. Radionuclide stability of alpha and beta emitters in urine for LSC analysis is an important consideration. The purpose of this work is to demonstrate optimal preparations and storage conditions of samples used for method verification.

Experiment on long-term storage of vegetable seeds under natural conditions

Preservation of the genetic diversity of crop plants and genetic collections of cultivated plants in a living state is an important state task. Seeds are a convenient form of storing genetic material, as the specimens are small in volume, require relatively little care, and remain viable for a long period. The genetically determined period of seed viability can be signifi cantly extended by creating optimal storage conditions. Therefore, it is necessary to search for or create environmental conditions under which seeds could remain viable for as long as possible. There are modern high-tech seed storage facilities in the world, but in the event of emergency situations, natural storage facilities, one of which is located on the Taymyr Peninsula, will be in a more advantageous position. Since 1974, an experiment has been carried out to study the safety of various products in diff erent types of packaging in permafrost conditions with the participation of a number of research institutions. Employees of the breeding laboratories selected seeds of 27 varieties of seven main types of vegetable crops; the samples were planted in triplicate, in accordance with the planned three extraction dates: in 2022, 2028, and 2034.

Influence of the storage conditions of embryo culture media on mouse development.

The culture of preimplantation embryos in vitro is an important method for human and mouse reproductive technology. This study aims to investigate the influence of different conditions of culture media on the preimplantation stage of mouse embryos cultured in vitro, and monitor the post-implantation development of new mice after embryo transfer to surrogate females. We demonstrated here that mouse embryos cultured in vitro in fresh M16, KSOM, Global, and HTF embryo culture media from one cell to the blastocyst stage and the subsequent embryo transfer to surrogate females are able to proceed through post-implantation development and, after birth, develop into healthy mice. However, culture of embryos in differently aged media shows various (often unpredictable) results. To find the optimal storage conditions of culture media, we suggest that the freezing and long-term storage of these media at - 80°C will not influence the quality of the media. To test this hypothesis, we grew embryos from one cell to blastocysts in vitro in the selected media after thawing and subsequently transferring them to surrogate females. Embryo culture in these four media after thawing does not affect preimplantation and postnatal mouse development. Thus, we have shown that storage of embryo culture media at low temperature (- 80°C) does not impact the quality of the media, and subsequently, it can be used for the culture of embryos for the full preimplantation period, the same as in fresh media.

GRAIN TEMPERATURE CONTROL IN METAL SILOS WITH LONG-TERM STORAGE

The temperature of grain in storage is a complex process determined by the interaction of various factors. It is important to establish effective monitoring and management systems to ensure optimal storage conditions and avoid negative consequences such as spontaneous combustion or product spoilage. Grain temperature plays an important role in maintaining its quality during storage. Temperature control is a key element to avoid problems such as spontaneous combustion, fungal and bacterial growth, and to ensure optimal storage conditions. Grain thermometry is an important part of monitoring grain storage and processing conditions in elevators and warehouses. Temperature plays an important role in maintaining quality and avoiding problems such as self-ignition. Various factors can affect the change in grain temperature during storage: climatic conditions, grain moisture, location of thermometers, grain condition, storage period, seasonal changes, level of ventilation, etc. Proper management of these factors is important to maintain grain quality and prevent potential problems such as spontaneous combustion or mold growth. We conducted a study of temperature changes in the formed layers of grain during its storage in metal silos. The influence of natural conditions and the location of silos on the storage of grain was established and recommendations were given for its storage in metal silos. Special attention should be paid to the contact zones with the surface of the grain embankment and the zones adjacent to the walls of the slurry, they are the most sensitive to temperature changes. The air temperature in the silo affects the temperature of the upper layers of the grain mass, which are in direct contact with it. When the ambient temperature rises, the air temperature in the silo rises, because the metal surfaces of the silo have high thermal conductivity.

Post-harvest cold shock treatment enhanced antioxidant capacity to reduce chilling injury and improves the shelf life of guava (Psidium guajava L.)

This study investigates the impact of storage temperature and cold shock treatment (CST) on the postharvest quality and shelf life of guava fruits. Guavas were stored at varying temperatures (4°C, 6°C, 8°C, and 10°C) for 25 days to evaluate the incidence of chilling injury (CI) and determine optimal storage conditions. Results indicated that the best storage temperature was 6°C, which effectively maintained fruit quality by inhibiting CI, controlling weight loss, and preserving soluble solid content and soluble sugars. Further analysis on the effect of CST on guavas showed that longer cold shock durations (6, 9, and 12 h) before storage at a constant 4°C improved the storage quality and shelf life significantly by decreasing the CI rates, reducing weight loss, maintaining firmness, and improving the fruit’s nutritional quality. Notably, a 12-h CST yielded the best outcomes, evidenced by the lowest malondialdehyde (MDA) content and the highest increases in proline and soluble protein content - biomarkers of enhanced cold tolerance and reduced CI. Additionally, CST was found to modulate the reactive oxygen species (ROS) metabolism, increasing the activity levels of key antioxidant enzymes such as superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX), alongside higher contents of ascorbic acid. The study identified a correlation between the length of cold shock and improved antioxidant capacity, crucial for extending guava’s shelf life and preserving quality during cold storage. These findings have significant implications for the postharvest management of guava, recommending 6°C storage and 12-h CST as effective strategies for extending shelf life and ensuring quality in cold storage settings.

Metabolomics and transcriptomics reveal molecular mechanisms of anthocyanin accumulation in 'Nanhong' pear (Pyrus ussuriensis) peel at different temperatures

The bright red hue of the 'Nanhong' pear's pericarp is a key sensory attribute, yet its susceptibility to discoloration at harvest poses challenges for maintaining its commercial appeal. This study, employs quality metrics, metabolomics, and transcriptomics to examine fruit quality and anthocyanin metabolism, along with gene expression involved in anthocyanin biosynthesis, under dark conditions at 0 °C, 10 °C, 20 °C, and 30 °C for up to 21 d. Findings indicate that 0 °C is optimal for long-term storage, while 10 °C is preferable for retail display. LC-MS/MS identified 42 anthocyanin metabolites cyanidin-3-O-galactoside (cya-3-O-gal) being the primary pigment in the pericarp. Discoloration was primarily associated with cyanidin-type and peonidin-type anthocyanins. Transcriptome analysis identified a large number of differentially expressed genes (DEGs) in different comparison groups, KEGG pathway analysis highlighted the significant enrichment of DEGs in phenylpropane and flavonoid metabolic pathways. Key genes in the anthocyanin synthesis pathway, including PuPAL, PuCHS, PuCHI, PuF3`H, PuANS, and PuDFR were markedly down-regulated at 20 °C and 30 °C. Transcription factors such as AP2/ERF, WRKY, MYB, NAC, C2H2, bHLH,and bZIP played an influential part in this process. The study also established that fruit senescence and high-temperature stress were the primary causes of pericarp discoloration at these temperatures. Consequently, identifying optimal storage conditions that preserve fruit quality, delay senescence, and sustain anthocyanin accumulation is crucial. In this study, storage at 10 °C demonstrated the most favorable outcomes for maintaining the pericarp's red color during the marketing period.

Profiling the antioxidant biomarkers in marine fish larvae: a comparative assessment of different storage conditions to select the optimal strategy.

Research on antioxidant biomarkers can generate profound insights into the defense mechanisms of fish larvae against different stressors and can reveal manipulation strategies for improved growth and survival. However, the number of samples to process and unavailability of required infrastructure in larval-rearing facilities limit the immediate processing, requiring the preservation of specimens. Silver pompano (Trachinotus blochii), a potential marine aquaculture species, shows a low larval survival rate due to poorly developed antioxidant mechanism. In this context, 39 storage conditions, including three storage temperatures and different buffers, were scrutinized to select the most suitable preservation strategy for five important antioxidant biomarkers of fish larvae, viz. catalase activity, superoxide dismutase (SOD) activity, measurement of lipid peroxidation, reduced glutathione (GSH), and ascorbic acid contents. The paper proposes the optimum larval storage conditions for these five evaluated antioxidant biomarkers to generate similar results in preserved and non-preserved larval samples. Larval samples preserved in PBS at lower temperatures (- 20°C and - 80°C) are recommended for evaluating catalase activity and ascorbic acid content. Catalase activity can also be evaluated by preserving the larval samples at - 20°C or - 80°C without buffers. Larval samples held in PBS or without any buffers at - 20°C and at - 80°C were found to be suitable for SOD and GSH evaluation, respectively. Preservation in 50% glacial acetic acid at - 80°C or - 20°C was preferred for the lipid peroxidation assays. Apart from methodological perspectives, the paper provides insights into the dynamics of larval antioxidant profiles of T. blochii, for the first time.

Development of a Decision Support System for Perishable Food Management in Culinary Education Institutions Using Machine Learning with Linear Regression

This study presents the development of a decision support system tailored for educational institutions with culinary programs, aimed at optimizing the management of perishable food items. The system employs a machine learning approach, specifically linear regression, to predict and enhance decision-making regarding the handling, storage, and utilization of perishable ingredients. The foundation of the system lies in the collection and analysis of historical data pertaining to various perishable food items commonly utilized in culinary education. Key variables, including temperature, humidity, storage duration, and other relevant factors, are identified to build a robust linear regression model. This model serves to predict the remaining shelf life of perishable goods, offering valuable insights into optimal storage conditions. Instructors benefit from a tool that enhances the practical learning experience, fostering a deeper understanding of food preservation and waste reduction. Continuous feedback mechanisms are integrated to improve the system's accuracy and relevance over time. Culinary educators can contribute insights, ensuring that the system remains aligned with the dynamic needs of culinary education.By incorporating this decision support system into culinary programs, educational institutions can not only empower students with practical skills but also in still a culture of sustainability and resource efficiency. The linear regression model, as a part of the system, aids in making informed decisions that align with industry best practices, contributing to the development of responsible and skilled culinary professionals. This research represents a significant step towards leveraging machine learning techniques to enhance culinary education, offering a practical and innovative solution for the management of perishable food items within educational settings.

Breaking dormancy in Cycas revoluta: A study of seed morphological characterization and dormancy mechanisms

ABSTRACT: Cycas revoluta seeds exhibit both physical and morphological dormancy, posing significant challenges to their germination. This study investigated the morphological characteristics and dormancy mechanisms of C. revoluta seeds and evaluated methods to overcome these dormancies. Seeds were harvested over two consecutive years and subjected to different treatments. A morphological analysis of seed structure was conducted, along with assessments of physical (seed size, weight, and moisture content) and physiological tests (germination rate, germination speed, and mean time of germination).Morphological characterization revealed that the thick, woody sclerotesta forms an impermeable barrier, while the embryos within are often underdeveloped at the time of seed maturity. Germination of the first year demonstrated that removing the sclerotesta significantly enhances germination rates, with naked seeds achieving a 70% germination rate at 30 °C. In the second year, soaking naked seeds in gibberellic acid at 200 ppm for 24 hours further promoted germination, though its effect was modest compared to sclerotesta removal (naked seed). This study also demonstrated that storing seeds for a year allowed embryos to complete their development, further improving germination outcomes. These findings suggested that mechanical removal of the sclerotesta, combined with optimal storage conditions, is an effective strategy for overcoming dormancy in C. revoluta seeds and soak time in gibberellic acid (200 ppm) or water for 24 hours could enhance germination as well.

Enhancing seafood freshness monitoring: Integrating color change of a food-safe on-package colorimetric sensor with mathematical models, microbiological, and chemical analyses

The study assessed a developed food-safe on-package label as a real-time spoilage indicator for fish fillets. This colorimetric sensor is sensitive to Total Volatile Base Nitrogen (TVB-N) levels, providing a correct indication of fish freshness and spoilage. This study evaluates and predicts the shelf-life and effectiveness of an on-package colorimetric indicator. The sensor, using black rice (BC) dye with polyvinyl alcohol (PVOH), polyethylene glycol (PEG), and citric acid (CA) as binders and crosslinking agents, is applied to PET films. The food-safe pH indicator, prepared via lab-scale flexography printing, is durable in humid environments, making it suitable for practical packaging scenarios. The sensor visibly monitored fish spoilage at 4 °C for 9 days. Quality assessment included tracking ΔRGB (total color difference), chemical (TVB-N, pH), and microbiological analyses. Results indicate that the fish samples are fresh up to 4 days of storage at 4 °C; the total viable count (TVC), Pseudomonas growth, TVB-N contents and pH reached: 5.2 (log CFU/ml), 4.31(log CFU/ml), 26.22 (mg N/100 gr sample) and 7.48, respectively. Integrating colorimetric sensor data with mathematical modeling can predict spoilage trends over time. Integrated system offers a smart approach to accurately predicting shelf-life, aiding in optimizing storage conditions, minimizing food waste, and delivering fresh, high-quality fish products to consumers.

Optimum storage conditions for ‘Abate Fetel’ pears

Optimum storage conditions for ‘Abate Fetel’ pears