The new western land and sea corridor makes use of a variety of transport modes to reach major ASEAN countries such as Singapore to the south, connects Southeast Asia and North America to the east, and connects Chongqing, Lanzhou and Xinjiang to the north with the China-European Union (CEU) liner train, which is a composite opening-up corridor in the western region for realising the regional linkage and international co-operation with ASEAN and other countries, and organically connecting with the "One Belt, One Road". In order to explore the regional freight network of Gan-Qing-Ning region of the new western land and sea corridor, we establish the model of node socio-economic attractiveness, topological charisma and comprehensive utility maximization, and analyze the index factors by using entropy weighting, Delphi and comprehensive evaluation methods through the Matlab Genetic Algorithm Toolbox to study the influence of node socio-economic attractiveness, topological charisma, node freight volume, logistics and transportation costs and construction costs on the utility of nodes of the new western land and sea corridor. The influence of the regional node utility of Gansu, Qinghai and Ningxia in the corridor. Analyze the problems and factors affecting the selection of nodes in the existing freight transport corridors in the three provinces. Completing the site selection of Gan-Qing-Ning regional node of the new western land and sea corridor and combining the import and export cargo volume of the three provinces to propose the construction of the South-Middle East three-lane freight corridor, and completing the optimization plan of the freight network. The results show that: scientific optimization and improvement of Gan-Qing-Ning regional freight network can promote the construction of freight network in the northwest region of the new western land and sea corridor, to meet the demand for freight transportation in the corridor, and to promote the development of the corridor industry and economic growth. Thus, it provides reference for the development of Gansu, Qinghai and Ningxia region and freight network optimization.