Optimal Medium Research Articles

Investigation into optimizing fermentation processes to enhance uric acid degradation by probiotics

To enhance the activity of Lactiplantibacillus plantarum FS4722 (L. plantarum FS4722) in reducing uric acid, this study optimized the culture medium components and fermentation conditions using response surface methodology. The results indicated that the optimal culture medium comprised glucose at 25.61g/L, yeast extract powder (YEP) at 26.86g/L, and nucleoside addition at 0.48g/L. The optimal fermentation conditions were: a fermentation time of 10h, an initial pH of 6.5, a fermentation temperature of 33 °C, and an inoculum size of 1.0%. Under these conditions, the nucleoside degradation ability of L. plantarum FS4722 increased by 41.9 times, enhancing its potential applications in reducing uric acid and developing anti-gout foods, health supplements, and pharmaceuticals.

Recent advances in lung cancer organoid (tumoroid) research (Review).

Lung cancer is the most critical type of malignant tumor that threatens human health. Traditional preclinical models have certain defects; for example, they cannot accurately reflect the characteristics of lung cancer and their development is costly and time-consuming. Through self-organization, cancer stem cells (CSCs) generate cancer organoids that have a structure similar to that of lung cancer tissues, overcoming to some extent the aforementioned challenges, thus enabling them to have broader application prospects. Lung cancer organoid (LCO) development methods can be divided into three broad categories based on the source of cells, which include cell lines, patient-derived xenografts and patient tumor tissue/pleural effusion. There are 17 different methods that have been described for the development of LCOs. These methods can be further merged into six categories based on the source of cells, the pre-treatment method used, the composition of the medium and the culture scaffold. These categories are: i) CSCs induced by defined transcription factors; ii) suspension culture; iii) relative optimal culture medium; iv) suboptimal culture medium; v) mechanical digestion and suboptimal culture medium; and vi) hydrogel scaffold. In the current review, the advantages and disadvantages of each of the aforementioned methods are summarized, and references for supporting studies are cited.

Performance investigation of a building-integrated collector based on spectrum splitting technology

Based on solar spectrum splitting technology, a novel concentrating photovoltaic/thermal (CPV/T) solar collector is proposed, which primarily composed of six Fresnel collector units with separate photovoltaic and photothermal modules. The compact overall structure is optimally designed that can be effectively integrated with ordinary buildings while supply both electrical and thermal energy. The proposed collector achieves the maximum annual average optical efficiency when installed north–south axially with a tilt angle approximately equal to the installation latitude, as a 64.97 % in Nanjing (32°N). Simulation analysis shows that the main influencing factors of the system performance are direct solar irradiance, working medium mass flow, and optical efficiency, the theoretical outlet working medium temperature can reach 150 °C while the photovoltaic and photothermal efficiencies are 12.37 % and 39.74 % when the normalized temperature difference T* is 0.06. Further, to verify the theoretical/simulation analysis results, the outdoor experimental tests are conducted and show that the optimal inlet working medium mass flow is 10.8 kg/h, while the efficiencies are 5.99 % and 27.59 %, respectively, that is, the proposed system comprehensive thermal efficiency can reach 43.35 %. The comparative analysis of theoretical research and experimental tests shows that the proposed system has rationality and practical value, which can provide guidance for practical engineering.

Perancangan Strategi Digital Marketing Pada Brand Sofia And Jenna Menggunakan Metode SOSTAC

The development of the Muslim Fashion Industry is increasingly rapid every year. This indicates that competition is getting tighter and the potential market for Muslim fashion in Indonesia continues to increase. Sofia and Jenna are one of the business actors operating in the Muslim fashion sector. Sofia and Jenna is a local brand that produces its own clothes (self-manufactured) which was founded in 2020. Even though it has been operating for several years, sales of Sofia and Jenna from January 2023 to October 2023 experienced fluctuations, in fact sales growth was still below Indonesian fashion industry. This is caused by several factors, namely low heart share and suboptimal use of digital marketing platforms, so this affects Sofia and Jenna's sales performance. For this reason, deeper observations were made regarding alternative solutions related to product, place, resource and promotion aspects. Of these four aspects, there is one aspect that shows the roots of the problem more than the other aspects, namely the promotion aspect. This research was conducted using the SOSTAC method which went through six stages, namely Situation Analysis, Objectives, Strategy, Tactics, Action, and Control. The aim of designing this digital marketing strategy is as a promotional medium and is expected to increase sales and marketing performance so that it can increase awareness of the Sofia and Jenna brand. Based on the digital marketing strategy design, this final project produced a design for the use of Social Media Optimization (SMO), Content Marketing, Partnership, Video Marketing, Linktree, User Generated Content (UGC), and Mobile Commerce Optimization tools. Then, the design is visualized using a Strategy Map. Through a structured and data-based approach, Sofia and Jenna are expected to be able to improve their position in the Muslim fashion market, as well as increase competitiveness and consumer loyalty towards the brand.

Assessing the mass-culture system and proximate content of Arthrospira maxima local for biogas and biohydrogen productions

Arthrospira maxima is a type of local cyanobacteria that has shown success in being grown massively in Indonesia. This research is aimed at looking at the resilience and ability to grow in outdoor conditions using cheap media and following environmental patterns in terms of temperature, sunlight and aeration. In specific purpose is utilization of the obtained biomass for biogas or biohydrogen production. Also, media optimization research from chemical pour grade analyzes into commercial types has been carried out at laboratory scale and field scale. The results show that at the scaling up process from laboratorial to outdoor condition the amount of biomass ratio has decreased by 2–10%, whereas proximate of protein has decreased from 46% to 38%, in contrast starch tends to increase from 10% to 23% and lipid was increase from around 9%–12%. Freshwater and marine water basic media also showing difference in ratio of biomass production and main proximate. The total calory and protein seems higher if A. maxima cultivate using marine water. Cultivation time outdoors turns out to be faster growth when compared to controlled conditions indoors. Biomass analysis results show that the largest content is protein, followed by carbohydrates and lipid. Considering the composition of the proximate, the cyanobacteria biomass tends to be used for biogas conversion rather than alcohol, due to the high protein with less carbohydrate content. Moreover, Arthrospira is cyanobacteria which is capable for producing heterocyst cells that can be excite gas. It was found the cyanobacteria release hydrogen gas around 54 μL/g wet biomasses/day mature culture. However, it should be remembered that this strain is still original or wild type, and can still be developed using both environmental and molecular manipulation approaches. Survival rate from laboratory scale to field scale shows that the microalgae is strong and worthy of consideration for industrial applications. Apart from that, high pH culture conditions are very good for removing contaminants. Also changing the media from pour analyses grade chemicals to commercial chemicals grade only reduces the biomass composition by 2–5% so it is worth considering for commercial applications. Testing various reactors to make biomass as needed with high productivity will be a challenge in the future.

A Nd-doped silica glass with high doping concentration, low hydroxyl content, and 900 nm fluorescence emission fabricated by laser additive manufacturing

The 900 nm wavelength laser is effective for atmospheric detection and various other applications.The frequency-doubled 450 nm deep blue laser can be used in maritime military, quantum optics, biomedicine, laser storage, laser display, and deep ultraviolet laser fields, among others. Although Nd3+ is considered the optimal medium for generating 900 nm lasers, competition with the 1060 nm emission wavelength must be addressed. In Nd-doped silica glass, without the introduction of aluminum, the emission intensity of the three-level transition is higher than that of the four-level transition. However, it can cause a concentration quenching between Nd3+, but co-doping with Al can effectively suppress it. As the Al/Nd ratio increases, the emission intensity at 1060 nm increases relative to that at 900 nm. By precisely adjusting the Al/Nd ratio, it is possible to suppress the 1060 nm emission while also inhibiting concentration quenching caused by Nd3+.

Statistical Optimization of Lipase Production by Aspergillus oryzae Using Olive Mill Wastewater

Among different sources of lipases, fungal lipases are hydrolytic enzymes owing much importance in industrial applications. However, the high production cost limits the industrial application of lipases. To address this issue, we have attempted to optimize lipase production by Aspergillus oryzae on olive mill wastewater (OMW) based medium. Media optimization is considered one of the best ways to economically produce a large quantity of lipase. The medium components were optimized by a statistical approach for optimal lipase production. The Plackett–Burman design was applied to identify the significant factors and response surface methodology to optimize medium components. The enzyme activity was highest under the following conditions: 3.49 g.L-1 glucose, 5.58 g.L-1 yeast extract and 0.72 g.L-1 CaCl2. A feasibility analysis of lipase production using the optimized data was performed, and at optimized conditions, lipase activity increased from 110.21 U.mL-1 to 212.53 U.mL-1, or an increase of 1.93 times.

Different Combinations of Nitrogen and Carbon Sources Influence the Growth and Postbiotic Metabolite Characteristics of Lactiplantibacillus plantarum Strains Isolated from Malaysian Foods.

Postbiotic metabolites produced by Lactiplantibacillus plantarum strains isolated from Malaysian food have been extensively reported for their positive effects on health. Understanding the effects of different combinations of carbon and nitrogen sources on the growth and corresponding characteristics of postbiotic metabolites produced by different strains of L. plantarum is important for various applications. Hence, the effects of different combinations of carbon (glucose, lactose, sucrose and dextrose) and nitrogen (X-SEED Kat, X-SEED Peptone, X-SEED Nucleo Advanced, Nucel875 MG, FM888 and FM902) sources on the growth of six strains of L. plantarum (RG11, RG14, RI11, RS5, TL1 and UL4) and the functional characteristics (bacteriocin inhibitory activity, antioxidant activity and lactic acid concentration) of their respective postbiotic metabolites were investigated in this study. UL4 produced the highest viable cell population with sucrose and Nucel875 nitrogen source. The UL4 strain also produced the strongest bacteriocin inhibitory activity with dextrose and FM888 nitrogen source. In comparison, the RI11 strain produced the highest lactic acid concentration with dextrose and Nucel875 nitrogen source and the highest reducing power of RS5 and TL1 postbiotic metabolites was achieved with MRS medium. In the combination of sucrose and X-Seed KAT nitrogen source, RG14 produce the highest hydroxyl radical scavenging activity. The effects of different combinations of carbon and nitrogen sources on the viable cell population of L. plantarum strains and the respective functional characteristics of postbiotic metabolites were strain dependent. The current study also revealed that fermentation media were an important factor that greatly impacted the functionalities of postbiotic metabolites due to the presence of various bioactive compounds that contributed to high antioxidant and antimicrobial properties. The results of this study will facilitate the subsequent medium design and optimisation for the development and production of specific postbiotic metabolites produced by the respective L. plantarum strain for their applications in various industries.

Statistical Optimisation of Streptomyces sp. DZ 06 Keratinase Production by Submerged Fermentation of Chicken Feather Meal

This study focused on the isolation of actinobacteria capable of producing extracellular keratinase from keratin-rich residues, which led to the selection of an actinobacterial strain referenced as Streptomyces strain DZ 06 (ES41). The Plackett–Burman screening plan was used for the statistical optimization of the enzymatic production medium, leading to the identification of five key parameters that achieved a maximum activity of 180.1 U/mL. Further refinement using response surface methodology (RSM) with a Box–Behnken design enhanced enzyme production to approximately 458 U/mL. Model validation, based on the statistical predictions, demonstrated that optimal keratinase activity of 489.24 U/mL could be attained with 6.13 g/L of chicken feather meal, a pH of 6.25, incubation at 40.65 °C for 4.11 days, and an inoculum size of 3.98 × 107 spores/mL. The optimized culture conditions yielded a 21.67-fold increase in keratinase compared with the initial non-optimized standard conditions. The results show that this bacterium is an excellent candidate for industrial applications when optimal conditions are used to minimize the overall costs of the enzyme production process.

A new evaluation method for sand control performance of slotted screen

Evaluation method of slotted screen performance is a critical concern for sand control. Despite its significance, comprehensive investigations into quantitative study of sand retaining during the complex flow process need further in-depth study, particularly in numerical simulation research model of the slotted screen. This paper adopts the two-way coupling calculation method of computational fluid dynamics (CFD) and discrete element method (DEM) for the particle movement and fluid flow at the slotted screen and establishes a numerical model of the sand particle migration process through the slotted screen. The sand particle deposition and plugging dynamics on the surface of the sand retaining medium and the micro sand plugging mechanism and blockage pattern are studied. The fractal dimension of formation sand is introduced to characterize different distribution of formation sand. The effects of fluid viscosity, width of the slotted screen, sand production concentration, fluid velocity and particle size distribution on sand production, sand passing rate and particle size distribution of formation sand passing through the slot and plugging behavior are studied. The sand plugging process of slotted screen can be divided into three stages: the beginning of blockage, the intensification of blockage, and the balance of blockage. Under simulated conditions, the initial sand plugging rate is 87%, and as the blockage intensifies, the sand plugging rate gradually increases to 96.5%. Based on the analysis of numerical simulation results, the index of total seepage resistance damage amplitude and velocity, general sand retention ratio and passed-sand size were defined to construct the integrated evaluation method of slotted screen media, which involves the performance of sand retention, anti-plugging and flow ability. A brand new set of ideas on quantifying the integrated evaluation method were introduced to calculate the individual and integrated performance indicators of the slotted screen based on simulated data. The quantitative evaluation of the sand control performance of the slotted screen provides guidance and reference for the parameter design of the slotted screen in the target oil region and the accuracy optimization of the sand control medium.

Optimization and Experimental Study of Iron Ore Grinding Medium Parameters Using EDEM Discrete Element Software.

Energy savings and consumption reduction of ball mills are crucial for industrial production. The grinding medium is an important component of a ball mill. In theory, using higher-density grinding media can yield better grinding results. However, for materials with varying grindability, employing grinding media of different densities can reduce energy consumption while maintaining the same grinding effect. This study simulates the motion of the grinding media in the mill using three different densities of balls and the same material (iron ore). The results reveal that balls with densities of 5.8 g/cm3 and 7.8 g/cm3 achieve faster grinding of materials into finer particles, but balls with a density of 5.8 g/cm3 consume less energy. Therefore, replacing a ball with a density of 5.8 g/cm3 in a ball mill can significantly reduce energy consumption. This study will assist in selecting the optimal grinding medium density for different materials, ultimately contributing to energy savings and reduced carbon emissions.

Optimization of the production of a new milk clotting enzyme by Aspergillus flavus thermophile on agro-industrial residues medium and characterization of the enzyme

This study focused on the optimization of the production of a milk clotting protease by Aspergillus flavus, isolated from a thermal station. The fermentation was carried out on wheat bran as a medium, followed by a partial characterization of the enzyme. The fungal isolate was screened for protease production based on clear zone hydrolysis on skim milk agar, and then it was identified as belonging to the genus Aspergillus based on its morphological characteristics. This identification was confirmed by molecular analyzes with 100% similarity with the A. flavus strain. After 7 days of fermentation, the crude extract is recovered by simple filtration. The production and optimization of the milk coagulant protease using wheat bran and agro-industrial waste as a medium was carried out using a statistical method of experimental design based on the Plackett and Burman matrices. The results showed that the optimal fermentation medium consisted of wheat bran (4% w/w), incubated at a temperature of 30 °C, with a pH of 5.0 after 6 days of fermentation. The enzyme was partially purified by ammonium sulfate precipitation, yielding an estimated proteolytic activity of 2,985 U. The partially purified protease exhibits optimal activity at pH 5 with hemoglobin as a substrate and an optimal temperature of 50 °C. The Lineweaver-Burk plot showed a KM value of 4.83 g/L and a Vm of 1,560 U. This enzyme causes very rapid coagulation of fresh cow’s milk with an optimum at 45 °C, a pH 7 and a concentration of 50 mM of calcium.

Effect of Media Formulations on Chlorophyll, Antioxidant Activity of Chlorella vulgaris and Its Potential as a Health Supplement

Graphical Abstract Highlight Research Chlorella indigenous from waters of Rokan River estuary was identified. The using of 4 chemicals KNO3, KH2PO4, MgSO4.7H2O and FeSO4.7H2O can reduce production cost and obtain an optimal medium formulation. The manipulated media was obtained can replace the commercial media of Chlorella Chlorella vulgaris contains high antioxidant activity. Abstract Chlorella is a microalga that is rich in chlorophyll and antioxidants so it has the potential to be a functional food or health supplement, however, the quality of Chlorella depends on the nutrient composition in cultivation. The research aimed to evaluate the effect of different formulations in Chlorella cultivation on the content of chlorophyll a, chlorophyll b, total chlorophyll, carotenoids and antioxidants. Furthermore, to analyze the profile of amino acids, fatty acids and secondary metabolism in the best formulation. The experimental design used was a non-factorial Completely Randomized Design (CRD) with 5 formulations in Chlorella cultivation: control, F-1, F-2, F-3, and F-4 by manipulating the use of 4 chemicals: KNO3, KH2PO4, MgSO4.7H2O and FeSO4.7H2O. The data obtained were analyzed descriptively and analysis of variance (ANOVA). The results showed that F-1 treatment with the use of 1.50 KNO3, 1.25 KH2PO4, 1 MgSO4.7H2O and 0.0498 FeSO4.7H2O (g L-l) was the best treatment with the content of chlorophyll a 38.19 µg/mL, chlorophyll b 41.45 µg/mL, total chlorophyll 79.65 µg/mL, carotenoids 0.08 µg/mL, and antioxidants activity 49.52 mg/L (strong) which is the same as the control treatment. In addition, Chlorella cultivated with the F-1 formula has 17 amino acid profiles with a total of 301.52 mg/g, 7 fatty acids 84.32 mg/g, and secondary metabolites, namely alkaloid 109.471 mg/L, flavonoid 82.111 mg/L, saponin 1342.222 mg/L, tannin 411,591 mg/L, and phenolic 151.889 mg/L. Therefore, the F-1 formulation can be developed for large-scale Chlorella cultivation and applied as a health supplement.

Efficient Plantlet Regeneration from Branches in Mangifera indica L.

Mango (Mangifera indica L.) is one of the most significant tropical and subtropical fruit species, with high ecological and economic value. However, research on the in vitro culture of mangoes is relatively weak, so establishing an efficient and stable mango plant regeneration system is of great significance. In this study, a preliminary mango regeneration system was established with Mangifera indica L. cv. Keitt from young branches as the starting explants. The results showed that the optimal plant growth regulator (PGR) formula for direct adventitious shoot induction on the branches was 1 mg/L 6-benzylaminopurine (6-BA) + 0.1 mg/L a-naphthaleneacetic acid (NAA), with an adventitious shoot induction rate of 73.63% and an average of 6.76 adventitious shoots. The optimal basal medium for adventitious shoot induction was wood plant medium (WPM), with an adventitious shoot induction rate of 63.87% and an average of 5.21 adventitious shoots. The optimal culture medium for adventitious shoot elongation was WPM + 1 mg/L 6-BA + 0.5 mg/L NAA, with an adventitious shoot elongation rate of 89.33% and an average length of 5.17 cm. The optimal formula for the induction of mango rooting was Douglas fir cotyledon revised medium (DCR) + 3 mg/L indole-3-butyric acid (IBA), with a maximum rooting rate of 66.13% and an average rooting quantity of 6.43. The genetic fidelity of the in vitro-regenerated plants was evaluated using inter-simple sequence repeat (ISSR) molecular markers. There was no difference between the in vitro-regenerated plants and the parent plant. This study provides an efficient and stable propagation system for Mangifera indica L., laying the foundation for its rapid propagation and genetic improvement.

Cellulase Enzyme Production Using Actinobacillus sp. on Several Alternative Growth Media

Cellulase enzymes are widely used in the food, beverage, animal feed, textile, and paper industries. The high cost of producing enzymes and low enzyme activity provides opportunities for using chemicals, and efforts are needed to produce cellulase enzymes economically through media optimization. This research aims to find alternative media that make it possible to produce cellulase enzymes with high activity. This research created a control media (M0) using pro-analysis chemicals and CMC as a carbon source. Three types of cellulolytic bacterial growth media were also created, consisting of technical chemicals, rice straw as a substrate, and different protein sources. M1: alternative media using urea as a protein source; M2: alternative media using powdered milk as a protein source; and M3: alternative media using chicken liver as a protein source. A total of 1% of cellulolytic bacteria (Actinobacillus sp.) was grown in each media and then incubated at 40°C for 33 hours at a speed of 140 rpm, and the production curve and cellulase enzyme activity were measured at every 3-hour interval. The results showed that the highest cellulase enzyme production was achieved at 24 hours using alternative media with powdered milk (M2) as a protein source. The cellulase enzyme activity produced was 2.9612 µ/ml.

A Novel Recombinant Vitronectin Variant Supports the Expansion and Differentiation of Pluripotent Stem Cells in Defined Animal-Free Workflows.

An essential aspect of harnessing the potential of pluripotent stem cells (PSCs) and their derivatives for regenerative medicine is the development of animal-free and chemically defined conditions for ex vivo cultivation. PSCs, including embryonic and induced PSCs (iPSCs), are in the early stages of clinical trials for various indications, including degenerative diseases and traumatic injury. A key step in the workflows generating these cells for more widespread clinical use is their safe and robust ex vivo cultivation. This entails optimization of cell culture media and substrates that are safe and consistent while maintaining robust functionality. Here, we describe the design of a human vitronectin (hVTN) variant with improved manufacturability in a bacterial expression system along with improved function in comparison to wild-type VTN and other previously characterized polypeptide fragments. In conjunction with an animal component-free media formulation, our hVTN fragment provides animal-free conditions for the enhanced expansion of iPSCs. This hVTN variant also supports the reprogramming of PBMCs into iPSCs. Furthermore, we show that these iPSCs can be efficiently differentiated into the three major germ layers and cortical neurons, thereby closing the loop on a completely defined animal-free workflow for cell types relevant for regenerative medicine.

Effect of Various Potting Media on the Growth and Root Development of Bougainvillea (Bougainvillea spp.) Cuttings

The study aims to enhance the propagation efficiency of Bougainvillea, a popular ornamental plant, by evaluating the effects of different potting media on its growth and root development. The experiment was conducted over 180 days using Bougainvillea hardwood cuttings and was arranged in a Randomized Block Design (RBD). The study revealed that a mixture of soil, sand, FYM, and vermicompost significantly improved plant height, stem girth, number of branches, number of leaves, leaf area, root weight, and survival rate compared to other treatments, highlighting its potential as an optimal potting medium for Bougainvillea propagation.The highest root length was recorded in the combination of Soil +Sand + Rice husk (1:1:1).

OPTIMIZATION OF CROSS-PLATFORM APPLICATIONS USING THE REACT LIBRARY

In today's world, with the growing use of mobile devices, the importance of creating cross-platform applications that provide high performance and quality of user experience has increased significantly. Improving the creation of such applications using React and React Native opens up wide opportunities for developers due to the possibility of code reuse, high development speed and ease of innovation, and is especially relevant because it concerns not only the technological aspects of development, but also economic efficiency, quality of user experience and the speed of introducing innovations to the software market. Open source and active community communication allow rapid identification and resolution of issues, development of new features and enhancements, and the sharing of knowledge and experience between developers. This creates a positive environment for innovation and growth, promotes rapid technological progress, and improves the quality of end products. The paper analyzes the key aspects and challenges associated with the creation of cross-platform applications, analyzes the main types of optimizations at different levels of development, including the component level, the state and data level, the level of working with APIs and external data, as well as the loading and code level. Special attention is paid to the integration of synchronous and asynchronous rendering to achieve optimal performance and user experience. Highlights the benefits of flexible state management using state managers, as well as the importance of image and media optimization to improve overall website performance. The article notes the role of the developer community in the process of improvement and innovation. Developers who master these technologies and optimize their application can create products that not only meet the requirements of the time, but also form new standards in the field of mobile and web development. Further research and integration of the latest technologies into the development process of cross-platform applications will have a significant impact on the software industry.

Efficient production of pullulan by Aureobasidium pullulans using a multi-objective optimization strategy with orthogonal experimental design coupling artificial neural network and genetic algorithm

Efficient pullulan production has long been a central research focus. This study used maltodextrin as the carbon source for pullulan production by Aureobasidium pullulans fermentation. A hybrid optimization approach, integrating orthogonal experimental design (OED), backpropagation artificial neural network (BP-ANN), and elite strategy non-dominated sequential genetic algorithm-II (NSGA-II), was developed. Range analysis based on OED revealed that MgSO4·7H2O significantly affects production but less impacts molecular weight, while pH notably influences molecular weight with a lesser effect on production, underscoring the need for multi-objective optimization. The BP-ANN model showed strong predictive capabilities, with goodness-of-fit values of 0.984 and 0.980 for production and molecular weight, respectively. Using this model as the fitness function for the optimization algorithm enhanced efficiency. Taking cost factors into account, the BP-ANN-NSGA-II algorithm identified the optimal fermentation medium conditions, resulting in a 6.89 % increase in production, a 368.97 % increase in molecular weight, and a 42.49 % reduction in cost. The maximum comprehensive optimization efficiency is 63.73 %, and the multi-objective optimization is better than the single objective optimization. This method significantly guides the improvement of pullulan fermentation optimization efficiency.

Cutting propagation of Salicornia europaea L. and the optimum NaCl and nutrient solution levels for its rooting and growth

ABSTRACT Salicornia europaea is a salty and crunchy succulent vegetable, traditionally known as precious gourmet food in Europe and Hawaii. Additionally, this plant is expected to be one of the most essential candidates for future crops under climate change because of its high salt tolerance and abundance of bioactive compounds. S. europaea is generally cultivated by seed propagation, which is challenging because of the heterogeneity in seed size, viability and dormancy. However, our results show that the cutting method is suitable for propagating this plant. We explored various nutrient conditions and proposed that the optimal culture medium conditions for promoting root and biomass development consists of 1.5% NaCl and a nutrient solution with 260 ppm N, 120 ppm P2O5 and 405 ppm K2O. Our findings suggest that the cutting method can facilitate S. europaea propagation, owing to its high degree of efficiency, homogeneity and simplicity, providing the potential to produce food even in salt-affected areas effectively.