The influence of wood fiber content on the road performance of asphalt mixture with high recycled asphalt pavement (RAP) content was studied based on the Marshall test, high temperature rutting test, low temperature crack resistance test and water stability test. And the optimum wood fiber content in RAP asphalt mixture with high RAP content was recommended. The results show that the dynamic stability and low temperature bending strain of RAP asphalt mixture increased and then decreased with the increase of wood fiber content. When the content of wood fiber is 0.3 %, the road performance of RAP asphalt mixture is the best. Compared with RAP asphalt mixture without wood fiber, the Marshall stability and low temperature flexural tensile strain of RAP asphalt mixture with 0.3% wood fiber increase by 7.1% and 4.6%, respectively, and the high temperature rutting resistance and water stability decreases.