Optimal Conditions For Production Research Articles

Metabolic engineering strategies for naringenin production enhancement in Streptomyces albidoflavus J1074

BackgroundNaringenin is an industrially relevant compound due to its multiple pharmaceutical properties as well as its central role in flavonoid biosynthesis.ResultsOn our way to develop Streptomyces albidoflavus J1074 as a microbial cell factory for naringenin production, we have significantly increased the yields of this flavanone by combining various metabolic engineering strategies, fermentation strategies and genome editing approaches in a stepwise manner. Specifically, we have screened different cultivation media to identify the optimal production conditions and have investigated how the additive feeding of naringenin precursors influences the production. Furthermore, we have employed genome editing strategies to remove biosynthetic gene clusters (BGCs) associated with pathways that might compete with naringenin biosynthesis for malonyl-CoA precursors. Moreover, we have expressed MatBC, coding for a malonate transporter and an enzyme responsible for the conversion of malonate into malonyl-CoA, respectively, and have duplicated the naringenin BGC, further contributing to the production improvement. By combining all of these strategies, we were able to achieve a remarkable 375-fold increase (from 0.06 mg/L to 22.47 mg/L) in naringenin titers.ConclusionThis work demonstrates the influence that fermentation conditions have over the final yield of a bioactive compound of interest and highlights various bottlenecks that affect production. Once such bottlenecks are identified, different strategies can be applied to overcome them, although the efficiencies of such strategies may vary and are difficult to predict.

Optimization of distillery-sourced wastewater anaerobic digestion for biogas production

Distillery industries in Ethiopia produces large volumes of high-strength, acidic, recalcitrant, and colored wastewater from sugarcane molasses-based alcohol distilleries. Interestingly, distillery wastes are high in organic nutrients that facilitate microbial growth and serve as substrates for renewable energy production. Conversely, energy insecurity is a major issue in the country, as access to modern energy services is limited in many parts of the country, particularly in rural areas. To address both challenges simultaneously, this study investigated the effects of temperature, pH, and retention time on distillery-sourced wastewater anaerobic digestion for biogas production. A Box-Behnken design confirmed that retention time influenced biogas production and biomethane yield largely. The numerical optimization of the process determined that the optimal conditions for specific biogas production (SBP) and biomethane yield were 31.21 °C temperature, 7.35 pH, and 16.85 days retention time. At this optimal point, the predicted values of SBP and biomethane yield were 0.469 l/g-COD removed and 57.87, respectively. The error percentages between experimental and predicted values were 1.71 % for SBP and 1.57 % for biomethane yield, suggesting the accuracy and predictability of the models. The study findings imply that simultaneous distillery waste treatment and energy production could be a viable option for providing affordable and clean energy to rural communities and small businesses in Ethiopia.

Synthesis and characterization of chitosan/polyvinyl alcohol immersed in sodium hydroxide thin film as a heterogeneous catalyst in biodiesel production

Biodiesel is a potential renewable energy source that can be used to replace fossil fuels because of its low toxicity, biodegradability, and renewability. However, biodiesel production requires long reaction times. Hence, a catalyst is needed to speed up the reaction and optimize production conditions. CS/PVA thin film catalyst immersed in NaOH has intriguing catalytic properties that can improve biodiesel conversion efficiency. In this paper, a series of chitosan/polyvinyl alcohol‑sodium hydroxide (CS/PVA-NaOH) thin films have been prepared as heterogeneous catalysts in biodiesel production. According to the FTIR spectra data, It shows that the vibrational frequency of the hydroxyl group (-OH) shifted from 3271 cm−1 to 3290 cm−1, indicating the hydrogen bond interaction between chitosan and PVA. Water uptake (45.42–218.64 %), swelling (120.63–142.99 %), porosity (62.67–243.43 %), and the durability of the thin film at pH conditions of 5–11 have been found to be the physicochemical characteristics of blended Chitosan/PVA (4/1; 3/2; 2.5/2.5; 2/3; 1/4 (v/v)). The optimal conditions for producing biodiesel were found to be around 65 °C for 90 min with an oil: methanol molar ratio of 1:7. By using a heterogeneous catalyst of CS/PVA-NaOH, the conversion efficiency from oil to biodiesel was achieved at around 95.39–99.52 %.

Detection and Environmental Optimization of dextran produced from Lactobacillus spp.

Introduction: Dextrans are D-glucose homopolysaccharides made by bacteria. There are several different bacterial species that produce these polysaccharides. Dextran is used as an adjuvant, drug carrier emulsifier, carrier, and stabiliser in the food, pharmaceutical, and chemical sectors, Nano gel. The aim of the study is to isolate Lactobacillus spp. from clinical sources, to identify the dextran production yield in Lactobacillus spp, and to reach the optimal growing parameters to discover the optimal dextran growth conditions. Methodology: 50 isolates of Lactobacillus spp. were gathered from different medical facilities in Baghdad City, 21 isolates came from healthy women's vagina (16 Lactobacillus plantarum, 5 Lactobacillus acidophilus), and 29 isolates came from samples of infant stool (15 Lactobacillus plantarum, 4 Lactobacillus acidophilus, and 10 Lactobacillus gasseri). All isolates were examined for dextran production using mucoid and spectrophotometric methods. Results: Lactobacillus acidophilus that was isolated from the genital tract of healthy women produced greater dextran. The results shows that the optimum conditions for dextran production that were investigated, there are several parameters play important roles to reach the optimal conditions, including, different natural carbon sources, nitrogen source, temperature, sucrose concentration, incubation time, pH, inoculum size. Conclusions: he optimum conditions for production were at 37 ºC for 48h at pH 5.5 with 4 % inoculum size and 6 g/ 100ml sucrose concentration with 6% best nitrogen source beef extract and the best natural carbon source for dextran production was dates.

Optimisation of Fungal Laccase Production from Monodictys castaneae

Laccases are copper-containing oxidase enzymes that have broad substrate specificity and applicability in industrial processes. The purpose of this work was to optimise laccase production by a certain endophytic fungus. Monodictys castaneae (Wallr.) Hughes was cultured and incubated using different nutritional and physiological factors affecting laccase production. It was found that the optimum physiological conditions for laccase production were 4 disc inoculum size, a 9-day fermentation period, pH 5, and 28°C. The enzyme was purified using acetone precipitation, gel filtration, and ion-exchange chromatography. The enzyme was identified as a monomeric protein with a molecular mass of 63 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Different concentrations of CuSO4 were used to stimulate laccase production and activity. A 0.15 g/l concentration of CuSO4 was the optimum dosage for laccase activity enhancement. For enzyme-guaiacol interaction, the optimal pH and temperature were 5.4 and 35 °C, respectively. The laccase activity was highly stimulated using 3 mM and 7 mM concentrations of chloride salts of Cd+2, Ca+2, Cr+2 and Fe+3, but the enzyme activity was slightly inhibited by 10 mM concentrations of these metal ions. According to all previous results, Monodictys castaneae showed promising potential as a producer of laccase.

Optimization of Oil Sorbent Thermoplastic Elastomer Microfiber Production by Centrifugal Spinning.

Fibrous structures are promising candidates for oil-water separation applications. In this study, we have produced poly(styrene-b-isobutylene-b-styrene) thermoplastic elastomeric fibers with the centrifugal spinning fiber production method. The optimal fiber production conditions were achieved when using a 25% w/w solution concentration in an 80/20 tetrahydrofuran/toluene (w/w) solvent system at 8000 rpm rotational speed. The produced fibers were bead-free and smooth-surfaced with a diameter of 3.68 µm. The produced fibers were highly hydrophobic and oleophilic, suggested by a water contact angle of 129° and the instantaneous absorption of the oil droplet. The oil absorption study showed fast absorption kinetics with 94% relative oil uptake after 1 min and a maximum of 16.5 g sunflower oil/g fiber. The results suggest that polyisobutylene-based thermoplastic elastomers could be promising alternatives in oil absorption applications.

In situ observation of photoelectrochemical water oxidation intermediates for selective biomass upgrading with simultaneous hydrogen production

In a water-splitting reaction, the anodic biomass upgrading with the simultaneous cathodic hydrogen production is a promising sustainable energy development for future needs. In the electrocatalytic water oxidation process, the reactive intermediates generated in the oxygen evolution reaction (OER) can be used for the selective oxidation of organic molecules to value-added chemicals. Herein, we fabricated a multifunctional catalyst, composed of phosphate-functionalized few-layer phosphorene (FLP-P) and bismuth ferrite (BFO) nanosheets (referred to as FLP-P-BFO), for photoelectrochemical (PEC) water-splitting reactions with the advantages of an efficient charge separation and the hybrid excitations of electrons and photons. At the cathode, the PEC-assisted FLP-P-BFO-catalytic reaction exhibits excellent hydrogen evolution reaction (HER) activity of a low overpotential of 110 mV at 10 mA cm−2 and a small Tafel slope of 51 mV dec-1. At the anode, the high FLP-P-BFO-catalytic efficiency is attributed to the bifunctional mechanism, in which the functionalized phosphate groups help to stabilize the Fe-OOH intermediate, thus mitigating the energy demand in the OER process. The bifunctional mechanism was validated by both pH-dependent and isotope-labeling examinations. With the assistance of in situ Raman spectroscopy, the optimal electrochemical conditions for the maximal production of the Fe-OOH intermediate in OER were obtained for the biomass upgrading of 5-hydroxymethylfurfural (HMF) to 2,5-furandicarboxylic acid (FDCA) with 100% conversion and selectivity, but without reducing the HER activity. The anodic biomass conversion, from HMF (a carbon–neutral feedstock) to the high value-added FDCA (a monomer of bio-plastics), with the simultaneous cathodic H2 production is a promising sustainable energy innovation. This novel strategy of employing PEC-assisted FLP-P-BFO-catalytic biomass upgrading to value-added products is extendable to combine the cathodic HER with many other anodic hydrocarbon oxidations for future energy-related applications.

Research progress on animal environment and welfare

AbstractAnimal welfare closely depends on its ability to adapt and thrive in a harmonious relationship with its environment, ensuring both physical and psychological well‐being. Over the years, the welfare of farm animals has gathered global attention and has become increasingly important to the general public and scientific communities. The connection between the environment and animal welfare is primarily established through the provision of suitable and controllable environment for animals. However, it is essential to recognize that the impact of environment extends beyond merely ensuring freedom from discomfort. The environment plays a crucial role in shaping an animal's response to challenges such as disease, stress, and pathogen. While animals may be housed in controlled environments that provide optimal conditions for health, production, and welfare, it is important to acknowledge that specific scenarios can significantly affect and alter the environmental requirements. Even with access to fresh air, certain factors can have a substantial impact on the well‐being of animals. Furthermore, providing appropriate environmental conditions goes beyond meeting basic needs and can greatly contribute to allowing animals to engage in their natural behaviors. It serves as a relevant tool for ensuring and maintaining adequate welfare standards. This review takes a comprehensive approach to environmental welfare by considering the welfare of animals managed in different stocking systems, considering environmental stress, stocking systems, and the provision of environmental enrichment items. By examining these factors, a broader understanding of the relationship between environment and welfare is achieved and recommendations for future research are outlined.

Kinshasa Province (Democratic Republic of Congo): Typology of Peri-Urban Ecosystems Providing Edible Insects

Tropical peri-urban ecosystems are essential for the urban population’s well-being through the goods and services they provide. Unfortunately, these ecosystems are subjected to anthropogenic pressures for various reasons. This study aims to assess the diversity and structure of the peri-urban ecosystems that provide the most edible insects in Kinshasa. A total of 360 people were interviewed to identify the two insect species most frequently consumed. The tree inventory was carried out in 50 plots (2500 m2 each), of which 25 were in Acacia plantations, 10 were planted Milletia forests, and 15 were natural Millettia forests. The diameters of each tree, dbh ≥ 10 cm, were measured. Principal component analysis (PCA) was used to distinguish the forest communities. Diversity indices were used to assess floristic variability and structural parameters were used to characterize forest stands. Two insects were identified as being the most commonly consumed in Kinshasa: Gonimbrasia jamesoni (28%) and Cirina forda (27%), found the in Acacia plantation, and in the planted Milletia forests and natural Milletti forest, respectively. The results indicate floristic (higher in the Acacia plantation) and structural variability between stands. The structure of the stands indicates good news for all forests, except the planted forest. The high frequency/dominance of A. auriculiformis and M. laurentii in the peri-urban ecosystems of Kinshasa would therefore be optimal conditions (under natural conditions) for good production of these two caterpillar species.

Prediction of glass phase viscosity in firing traditional ceramics and porcelain tiles with waste glass powder

The theoretical calculation of the viscosity of traditional ceramic materials is complex and models based on glass processing are generally used to estimate the viscosity of the glass phase according to its chemical composition. FactSage model considers structural information from silicate melts (glass phase), which is a different approach compared to other models. The viscosity of the melt fraction was calculated for different traditional ceramics, such as hard porcelain, technical porcelain, sanitary ware and porcelain tiles, including formulations with partial replacement of the fluxing raw material by soda-lime glass waste. FactSage model consistently indicated an increase in viscosity with higher levels of silica and a decrease in viscosity with the utilization of soda-lime glass waste in the formulations. Different responses and a nonlinear behavior were presented when varying the Na2O/K2O contents in the glass phase. The tested models presented reliable results for the processing of molten glass, but depending on the chemical composition and temperature, significant differences were observed among the values. Despite this, models that simulate the viscosity of the glass phase can help to optimize production conditions, but more investigations are needed to improve the relationship between theoretical and experimental results of viscosity.

Isolation and characterization of probiotic bacteria from fruit pulp, screening of probiotic properties and production of bacteriocin and probiotic curd

The present study was investigated to isolate probiotic lactic acid bacteria from Actinidia deliciosa (kiwi fruit) pulp. A total of eight isolates were found and two of them were probiotic LAB strains. The both A2 and A5 isolates strains were Gram-positive, catalase and oxidase negative, non-spore forming, non-motile, rod or short cocci shaped bacteria. The A2 and A5 strains both survived well in conditions of low pH, 0.4% phenol and high bile salt concentrations. They could grow at different temperatures and were resistant to different NaCl concentrations. A study was also conducted on the various probiotic and safety attributes of isolates. Bacteriocins, a kind of ribosomal protein produced by LAB, were estimated using the Folin-Lowery method. The formation of bacteriocins was influenced by various physical and chemical factors. The result demonstrates that the optimum conditions for the production of bacteriocins by the A2 strain were glucose as carbon source, pH 6.5, 2% NaCl and 1% bile salts. For the A5 strain, sucrose as a carbon source, pH 6.5, 1% NaCl and 1% bile salt were optimum conditions for bacteriocin production. Study was also conducted on exopolysaccharide (EPS) production, adhesion characteristics, antagonistic activity and antibiotic sensitivity. Probiotic curd (Dahi) formation was done and several parameters of curd like pH, total lactic acid concentration and water-holding capacity were all investigated.

Yield and Technological Quality of Pirarucu Trimming Surimi According to the Number of Washing Cycles, Rice Flour Content, and Cooking Temperature.

This research aimed to optimize surimi production with innovative raw materials called pirarucu trimmings and broken rice grains, which are by-products from food industries. The effects of three independent variables (number of washing cycles, content, and cooking temperature of rice flour-RF) on surimi's physical, chemical, and technological qualities were investigated through a Box-Behnken design. The number of washing cycles affected yield (77-93%), moisture (55-67%), lipids (18-35%), protein (7.15-11.88%), whiteness (46.73-64.45), chroma (8.86-13.18), hue angle (80.79-93.12°), cohesiveness (0.40-0.61), springiness (0.51-0.99), and freeze stability after 4 weeks (85.16-96.53%). RF concentration affected moisture, lipids, cohesiveness, springiness, and freeze-thaw stability after 4 weeks. RF cooking temperature affected moisture, chroma, cohesiveness, and springiness. The optimal conditions for surimi production with high yield and overall quality were three washing cycles, 6% of RF, and RF cooking temperature at 85 °C. It reveals the promising potential of both by-products to be used as an ingredient in restructured products and contribute to improving agri-industry sustainability.

Effect of different carbon sources on exopolysaccharide production of Lactiplantibacillus pentosus KS27 and optimization of production conditions using Box-Behnken designing

Effect of different carbon sources on exopolysaccharide production of Lactiplantibacillus pentosus KS27 and optimization of production conditions using Box-Behnken designing

Study on the mechanical strength and iodine adsorption behavior of coal-based activated carbon based on orthogonal experiments

To prepare coal-based activated carbon with high mechanical strength and adsorption performance for the removal of iodine from wastewater, the effects of carbonization temperature, heating rate, activation time and activator on the mechanical strength of activated carbon were studied; the optimal production conditions were determined by comprehensively considering the specific surface area, iodine value, compressive strength and wear resistance based on previous studies. Iodine adsorption kinetics and equilibrium adsorption experiments were carried out on activated carbon prepared under the optimum conditions. The results showed that the increase in mechanical strength was caused by the release of volatile matter and secondary reactions, which formed the carbon skeleton, while the decrease in mechanical strength was due to the intervention of the activator, which destroyed the carbon skeleton. The adsorption kinetics showed that a pseudo-second order kinetic model was more suitable for the adsorption behavior of iodine on activated carbon. The equilibrium adsorption experiment results showed that iodine was evenly adsorbed on the surface of activated carbon, and with increasing temperature, the affinity of the active sites of activated carbon to iodine also increased.

Optimization Condition for Ethanol Production from Sweet Sorghum Juice by Recombinant Zymomonas mobilis Overexpressing groESL Genes

High-temperature ethanol fermentation (HTEF) using high-potential thermotolerant ethanologenic microorganisms is a promising platform for ethanol production in tropical or subtropical areas. This study aims to evaluate the ethanol production potential of recombinant Zymomonas mobilis R301 overexpressing groESL genes under normal and high-temperature conditions and the expression of genes involved in the heat shock response and ethanol production pathway during ethanol fermentation using sweet sorghum juice (SSJ) as feedstock. Growth characterization analysis revealed that the recombinant Z. mobilis R301 exhibited multi-stress tolerance toward heat, acetic acid, and furfural. Based on the statistical experimental design, the optimum conditions for ethanol production from SSJ by the recombinant R301 at 30 °C were a sugar concentration of 171.67 g/L, cell concentration of 9.42% (v/v), and yeast extract concentration of 10.89 g/L, while those at 40 °C were a sugar concentration of 199.48 g/L, yeast extract concentration of 10.88 g/L, MgSO4 concentration of 1.05 g/L, and initial pH of 6.8. The maximum ethanol concentrations and productivities achieved in this study were 63.26 g/L and 1.17 g/L.h at 30 °C and 58.62 g/L and 1.22 g/L.h at 40 °C. The overexpression of the groES and groEL genes and upregulation of other heat shock-responsive genes at 40 °C enhanced cell growth, viability, and fermentation capacity of recombinant Z. mobilis R301 under heat stress. The current study demonstrated that recombinant Z. mobilis R301 exhibited high potential for ethanol production from SSJ or other sugar-based raw materials under high-temperature conditions.

Box–Behnken optimization of biodiesel production using trisodium phosphate catalyst from monazite ore processes

AbstractTrisodium phosphate (TSP) is a non‐toxic waste obtained from alkaline cracking as part of the processing of monazite ore. After simple purification, TSP can be utilized in many applications; however, it is used as a catalyst in this study. It is characterized using Thermogravimetric analysis , X‐ray diffraction analysis, Brunauer‐Emmett‐Teller, and Temperature‐programmed desorption of carbon dioxide and optimized in the transesterification reaction. After recrystallization and calcination at 350 °C for 0.5 h, the form of the TSP was tetragonal, with a 2.61 m2/g surface area and 537.4 x 10‐6 mol/g basic sites. Trisodium phosphate can therefore be utilized effectively as a solid base catalyst for the transesterification of palm oil. Three biodiesel production parameters were investigated: the methanol to oil molar ratio (6:1–24:1), catalyst concentration (1–10%), and reaction time (30 to 240 min). The experiments were designed using the Box–Behnken response surface method. As a result, the optimum conditions for biodiesel production are a 22:1 methanol to oil molar ratio, 4% catalyst concentration, and 200 min reaction time. The optimal fatty acid methyl ester (FAME) content is 99.79%, with an error between actual and predicted FAME of 0.21%. © 2023 Society of Industrial Chemistry and John Wiley & Sons Ltd.

Determination the Optimum Conditions for β-glucan Production Extracted from Saccharomyces cerevisiae

Β-glucan is a natural polysaccharide composed of a group of glucose monomers linked by beta glycoside bonds that yeasts can be synthesized. The effect of different temperatures, inoculum size, glucose concentration, yeast extract concentration, pH, and culture media on the β-glucan production from saccharomyces cerevisiae was studied. In addition, the dry weight of biomass containing β-glucan was measured. The results showed that the optimum conditions for β-glucan production were when the cells were grown at 30 ºc for 48 hours and pH = 7 with 10 % inoculum size, 2% glucose, and 0.1% yeast extract. Furthermore, the best culture medium for β-glucan production was yeast extract-glucose medium with a dry weight of glucan 2.5mg/5ml. In addition, this study aimed to determine the best method for β-glucan extraction with acceptable purity and concentration. Β-glucan was extracted using two methods autolysis and alkali-acids extraction. The dried and weighed crude β-glucan was 6 gm/l, and 8 gm/l for autolysis of yeast cells, and alkali-acids extraction, respectively. The analysis of glucan by FT-IR and HPLC showed that the method of extraction by autolysis of yeast cells was successful to obtain β-glucan without the presence of the α-glucan with a concentration of β-glucan 10.8mg/gm, while in the extraction by alkali-acid were obtained mixtures of α-glucan and β-glucan with the concentration of β-glucan 7.4 mg/gm. The current study revealed that the optimum conditions of β-glucan production and the best culture medium for β-glucan production was yeast extract-glucose medium and the best method for β-glucan extraction was autolysis extraction.

Screening, Identification, and Optimization of Enzyme-Producing Conditions for Cellulose-Degrading Bacteria in Distillery Lees

Cellulose-degrading bacteria were identified from distillery lees, and the strains were optimized for fermentation and enzyme production, providing effective strains for the resource utilization of distillery lees and developing cellulase. Based on the univariate test, the response surface test was used to optimize enzyme production conditions for fermentation. The screened strain JZ2 had a clear circle-to-colony diameter ratio of 2.0. The enzyme activities of exoglucanase, endoglucanase, and β-glucosidase were 4.341 ± 0.05 U/mL, 1.874 ± 0.04 U/mL, and 0.739 ± 0.02 U/mL, respectively. The bacterial colonies were large, and the bacterial cells’ morphology was rod-shaped. We identified Bacillus cereus (JZ2) from 16S rDNA sequence homology and phylogenetic tree analysis as belonging to the genus Bacillus in the thick-walled Bacillus phylum. The optimum production conditions of cellulase from strain JZ2 were a fermentation time of 2 d, a rotation rate of 180× g, and a temperature of 26 °C using the response surface method. The enzyme activity of JZ2 was 4.625 U/mL under optimal enzyme production conditions. In addition to good activity, the cellulase from the JZ2 strain may have the potential to convert distillery lees cellulose into useful compounds.

Toxicity of Bikaverin to Some Pathogens and Optimal Production Conditions

Bikaverin is a reddish pigment produced by a variety of fungal species, the majority of which belong to the Fusarium genus. This pigment has antibiotic activities against protozoa, bacteria, and fungus. This study looked at the toxicity of Bikaverin to some microbes, production, and characterization of the pigment from the fungus Fusarium oxysporum. The yield of total bikaverin in F. oxysporum shake flask culture was improved by optimizing cultivation variables such as complex medium, carbon supply, nitrogen source, temperature, medium pH, and incubation period. The maximum production of complete bikaverin was found after 10 days at 28°C in Potato dextrose broth [PDB] supplemented with 2% glucose, 2% corn step liquor, and a pH of 5.5. The isolates of more sensitive bacteria to bikaverin were Staphylococcus aureus and S. typhi, with inhibition zones 24 and 23 mm respectively, at a concentration of 75 ?g/mL. The isolates more sensitive fungi to bikaverin were E.faecalis, E. coli, C. albicans, and P. aeruginosa, with inhibition zones 18, 22, 20 and 13 mm, respectively, at concentrations of 75 ?g/mL. The study concluded that the toxic antimicrobial activity of bikaverin was against fungi and bacteria

Microalgae Cultivation Using Municipal Wastewater and Anaerobic Membrane Effluent: Lipid Production and Nutrient Removal

Microalgae cultivation using wastewater is a combined process for pollutant removal and lipid production that has been widely studied in recent years. In this study, the effects of anaerobic membrane effluent (AME) and municipal wastewater (MW) ratios on microalgae growth and pollutant removal processes were investigated, and the lipid production properties were also explored. Results show that microalgae can grow in all AME/WW ratios, and a 40% AME content is the optimal condition for microalgal biomass accumulation (52.9 mg/L·d) and lipid production (0.378 g/L). Higher AME addition would inhibit microalgae growth. In addition, high ammonia (approximately 97%) and phosphate (around 90%) removal efficiencies can be achieved in all AME/WW ratio conditions, while the total nitrogen removal efficiencies decreased with the addition of AME. Total nitrogen and phosphate are the limiting factors in treating water to meet the requirements of the integrated wastewater discharge standard. This study provided a new method for anaerobic digestion and municipal wastewater treatment and also realized green energy production based on the sustainable development principles.