Microbial fuel cells (MFCs) are a promising technology in bioelectricity production. Water systems may be utilized in producing electricity by bio-electrochemical catalytic activity of its inherent microbial culture while simultaneously treating wastewater. Current studies are focusing on design and material optimization for future up-scaling application. For large-scale application, optimization studies such as compartmentalization and stacking become important. In this study, a membrane-less microbial fuel cell is designed and optimized in terms of optimum electrode distances and optimum surface area ratios. It was found that the specific design yielded a maximum of 25.81 mV at the optimum distance wherein dissolved oxygen is sufficiently low enough in this level. Through the optimization of electrode distance was also found that the MFC designed is anode-limited with a 1:4 ratio of anode to cathode is required to produce its maximum power density output. Multiple electrodes study proves the MFC setup is stackable even without membrane separation. This paper reports the first known attempt to quantify an optimum surface area to volume ratio at 2.34 m2/m3.