A successful migration from current C-band based optical networks to a multiband scenario primarily depends on the development of solutions that can reliably measure physical properties of optical links over broad spectral transmission windows. Additionally, these solutions must be capable of delivering wavelength-dependent and spatially-resolved indicators that can empower network operators to identify faults before they lead to severe service disruptions. Recently, the exploitation of receiver based digital signal processing as a tool for optical performance monitoring has gained tremendous popularity. One successful example is the so-called <italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">in-situ</i> power profile estimator, which can reconstruct the per-channel longitudinal power profile along the optical fiber link solely processing the received signal samples. In this work, we propose a novel application for the <italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">in-situ</i> power profile estimator by harnessing it on multiple wavelengths to accurately estimate the spectral gain profile of C+L-band in-line Erbium-doped fiber amplifiers deployed in a 280-km single mode fiber link. Furthermore, we show how this scheme can be efficiently used to detect amplification-related anomalies, such as gain tilt and narrowband gain compression. In our measurements, we achieved a sub-dB estimation accuracy by comparing the proposed gain extraction approach with the back-to-back characterization obtained from an optical spectrum analyzer.