Motivation Human rights non-governmental organizations (HR NGOs) play a significant role in the protection and promotion of human rights, particularly in the global south. In Bangladesh, human rights violations are a major concern. The government's approach to helping victims of abuses and upholding human rights has become contentious. Therefore, in this context, NGOs' approach to and activism in helping victims, public education, and putting pressure on the government to uphold human rights have been considered vital. Purpose The article evaluates and explores the key challenges HR NGOs in Bangladesh face in safeguarding human rights. Methods and approach For this qualitative study, primary data was collected through in-depth interviews with 25 human rights activists, victims of human rights violations and their family members, journalists, NGO officials, and academics. The 25 participants were selected on the basis of purposive and snowball sampling techniques. Findings The findings suggest that the overall role of HR NGOs in Bangladesh is dubious. The HR NGOs in Bangladesh cannot play an effective role in promoting human rights, and the government's role and its responsibility to help victims and safeguard human rights is not upheld. Bangladesh's government tries to limit the function of human rights groups. The key challenges faced by HR NGOs include the ruling elite's intervention in their activities, the government's oppression and harassment of NGO officials, the country's culture of impunity, NGOs' dependence on foreign donations, restrictive laws used to control the growth of HR NGOs, the HR NGOs' financial condition and political interest, and the ineffectiveness of human rights institutions in Bangladesh. These challenges restrict the growth of the HR NGOs in the country and result in their poor performance. However, although their role and influence are limited due to the many forms of government control and oppression, HR NGOs appear to be more active than the government in upholding human rights. Policy implications The collective efforts of the HR NGOs, civil society, the government, and general citizens are crucial to protect and support human rights. HR NGOs, civil society organizations (CSOs), journalists, and the general public should not remain silent on gross violations of human rights but should make the utmost effort to encourage citizens to break the culture of impunity and raise their voices against human rights abuses. In this regard, citizens need to understand the concept of human rights. The state's co-operation in protecting human rights is critical, as the fundamental duty of the state is to ensure the rights of its citizens. Human rights NGOs should monitor human rights situations, help victims of violations, and engage in popular education. The voices of HR NGOs and CSOs should be neutral and united in order to exert enough pressure on the government to change its behaviour regarding human rights. In addition, the judiciary, NHRC, and Anti-Corruption Commission (ACC) should play an active role in ensuring equality and justice.