ABSTRACTWe prove that if is a conditionally negative definite matrix with all entries positive and is a non-constant operator monotone function, then is also conditionally negative definite. Moreover, is invertible if A is invertible. Next, we prove that if is a symmetric matrix with all entries positive and only one positive eigenvalue, and for all , then also has only one positive eigenvalue and it is invertible if A is invertible. Analogous results are also considered when the diagonal entries of A are all zero and off-diagonal entries are all positive, which extends a result of Reams.