Objective: One of the most important components of patient safety is safe surgical practices. Presence of risky surgical patients and units, and the increased number of surgical interventions applied to patients globally increase the responsibility of surgical nurses in terms of patient safety culture. The aim of this study was to determine the perception of patient safety culture of surgical nurses and the factors affecting it. Methods: The data of this descriptive and cross-sectional study were collected with the voluntary participation of nurses who worked in the surgical units of two public hospitals and one university hospital. This study aligns with the STROBE (Strengthening the Reporting of Observational Studies) checklist. Results: Nurses who received training on patient safety and nurses who are satisfied with their working conditions had a more positive patient safety culture perception. It was determined that the nurses working in the surgical services attach more importance to cooperation and comply with the policies to ensure patient safety compared to the operating room nurses. Conclusions: The perception of the nurses regarding patient safety culture was found to be moderate. It is recommended to maintain training opportunities and good working conditions on patient safety and to provide management support.