Carbon neutral fuels must have a control over global warming. Even through passenger vehicle can be replaced with electrical and hybrid vehicles, but it is extremely difficult to replace goods transport vehicles which uses hydrocarbon fuels. Biofuels are one that can be obtained from various feedstock’s including grains and green matter with high starch and sugar content such as corn, sugar cane and sugar beets. The castor oil methyl esters, which is non-edible in nature fulfils the requirement of fuel for internal combustion engine. The conventional experimental scheme needs more time for optimization and substantial number of experiments need to perform as it is possible to vary a single operating variable at a time and is expensive. Mathematical models of Taguchi method using design of experiments (DOE) give superior results. By using DOE, Taguchi L9 orthogonal array is considered. Analysis of variance (ANOVA), The Regression Equation and signal-to-noise (S/N) ratio are obtained to predict the best parameters and to evaluate the influence of significant conditions on performance, emission and combustion characteristics. The mathematical model obtained by integration of Taguchi method and RSM is successfully validated with accuracy of 95%.