The Indigenous bio-bed technology with electrical cells were designed to evaluate the Nitrogen removal performance and other pollutants from the river water. The designed Indigenous bio-bed technology were taken an influent levels of CODcr at 250 mg/L, NH4-N and, NO3-N concentrations at 7.5 mg/L for the experiment. The device ran for 48 h for nitrogen remediation of the river water, thus, achieving removal efficiency of 85 %, 97 %, 98 %, 95 % and 92 % for CODcr, NH4-N, NO2-N, NO3-N and TIN, respectively. The highest power and current density generated were 486.86 mWm−3 and 4807.02 mA/m3, respectively. The correlation of both the values of Voltage production and TIN removal performance is shown to be statistically as, R2 of 0.9861 and 0.9999 of both open and close circuit mode of operation, respectively. The dominant phylum relative abundance deduced the close circuit mode of operation: higher relative abundance than the open circuit.