Open Time Research Articles

Preparedness and Public Health: an Italian Local Health Authority plan for a mass gathering in 2023

Abstract Issue Mass gatherings represent an important Public Health challenge. The increased number of people present in a specific area puts local health systems under stress (i.e.workload of emergency services, environmental health and food safety) and at the same time it rises the risks of a potential mass casualty incident. Effective planning and disaster preparedness have to be achieved through collaboration among local health authorities(LHA) and other stakeholders (event planners, law enforcement, local transportation services). Problem Every year, for 3 days, the Italian Alpine troops gather in a different city to remember First World War fallen. During those days, the city centre transit is not allowed to car (in specific areas even to emergency vehicles).In May 2023 this event occurred in Udine, a city in the North East of Italy with almost 100.000 inhabitants. For that year at least 300.000 participants were expected. LHA had to reorganize preparing itself to manage a temporary quadrupled catchment area. Results In the previous months, local authorities met local health representatives and other stakeholders in order to define a single emergency response plan. For healthcare services it was decided to: institute first aid points within the city; reorganise patients’ emergency transport system; increase hospitals’ treatment capacity (beds, life support equipment); increase healthcare professional availability; empower drugstores (opening times, drugs procurement); strengthen human and animal health surveillances;activate alternative communication systems. During the event, LHA updated other authorities involved three times a day about hospital and emergency system situation. At the end of the event,a multiprofessional debriefing meeting was held and no critical situations were reported. Lessons Preparedness requires a great commitment in terms of resources and time, but it is confirmed to be essential to guarantee adequate assistance even in extraordinary conditions. Key messages • Mass gathering s events put under stress all the main areas of public health system. • Public health preparedness cannot be effective without a strong involvement of other public and private stakeholders.

Effect of acupuncture on AMPK/ULK1 signaling pathway of hippocampus in chronic stress-induced depression rats

To observe the effect of manual acupuncture stimulation on changes of behavior and hippocampal signaling pathways of AMP-activated kinase (AMPK)/UNC-51-like kinase 1 (ULK1) in rats with chronic unpredictable mild stress (CUMS)-induced depression, so as to explore its molecular mechanism underlying improvement of depression. Thirty-two male SD rats were randomly divided into normal control, model, acupuncture and medication (fluoxetine) groups, with 8 rats in each group. The depression model was established by using CUMS (fasting, water depredation, restraint, swimming in cold water, crowding, stroboscope stimulation, and tail clipping, each of which was used once a week) for 6 weeks. Manual acupuncture stimulation was applied to "Baihui"(GV20) and "Yintang"(EX-HN3) for 10 min, once daily, 6 times a week for 6 weeks before every-day modeling. Rats of the medication group received gavage of fluoxetine (10 mg/kg) once a day for 6 weeks before every-day modeling. The percentage of sucrose preference, and the percentages of open arm times and open arm time in the elevated plus maze experiments were detected. The expression levels of AMPK, phosphorylated (p)-AMPK, ULK1, p-ULK1, mammalian target of rapamycin (mTOR) and p-mTOR proteins in the hippocampus tissue were detected by Western blot, and the expression of autophagy effecting protein (Beclin-1) and the selective autophagy receptors (p62) mRNA in the hippocampal tissue was detected using real-time fluorescence quantitative PCR, respectively. Compared with the normal group, the percentage of sucrose preference, percentage of times of entering the open arm and percentage of open arm time, the expression levels of p-AMPK /AMPK ratio, p-ULK1 /ULK1 ratio of proteins, and Beclin-1 mRNA were significantly decreased (P<0.01, P<0.05), and the expression levels of p-mTOR /mTOR ratio and p62 mRNA were significantly increased (P<0.01, P<0.05) in the model group. In comparison with the model group, the percentage of sucrose preference, percentage of open arm times and open arm time, the expression levels of p-AMPK /AMPK ratio, p-ULK1/ULK1 ratio of proteins, and Beclin-1 mRNA were significantly increased in both acupuncture and medication groups (P<0.01, P<0.05), while the expression levels of p-mTOR /mTOR ratio of proteins, and p62 mRNA were markedly decreased only in the medication group (P<0.05). Acupuncture treatment can alleviate depression-like behavior in CUMS rats, which may be related to its functions in up-regulating AMPK/ULK1 signaling pathway to induce cellular autophagy in the hippocampus.

NAGK regulates the onset of puberty in female mice

This study examines the role of N-acetylglucosamine kinase (NAGK) in initiating puberty in female mice. We employed real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) and immunofluorescence to measure NAGK expression in the hypothalamic-pituitary-ovarian axis across various developmental stages: infant, prepuberty, puberty, and adult. We further investigated the impact of Nagk gene knockdown on puberty in female mice. This included assessing the expression of puberty-related genes both in vivo and in vitro, GT1-7 cells proliferation and apoptosis, concentrations of GnRH and Kisspeptin, puberty onset timing, serum levels of progesterone (P4) and estradiol (E2), and ovarian morphology. Results revealed that Nagk mRNA is present in the hypothalamus, pituitary, and ovaries throughout different developmental stages in female mice. In the hypothalamus, Nagk mRNA levels were comparable during infant and prepuberty, lowest during puberty, and highest in adult. In the pituitary, Nagk mRNA peaked in adult, with no significant variation between infant, prepuberty, and puberty. In the ovaries, Nagk mRNA levels increased during puberty and peaked in adult. NAGK is predominantly located in the arcuate nucleus (ARC), periventricular nucleus (PeN), dorsomedial hypothalamic nucleus (DMH), paraventricular nucleus (PVN), adenohypophysis, and in the ovarian oocytes, interstitium, and granulosa cells across all developmental stages in female mice. Nagk knockdown in GT1-7 cells decreased the transcriptional level of Gnrh, Kiss1, Gpr54, Igf1 and Mapk14 mRNA and cell proliferation but increased the level of β-catenin mRNA and cell apoptosis, while reducing GnRH secretion. Following ICV injection, Nagk gene knockdown mice exhibited delayed the timing of vaginal opening (VO) and reduced hypothalamic levels of Gnrh, Kiss1, Gpr54, Igf1, Mapk14, and β-catenin mRNA. Additionally, serum concentrations of E2 in Nagk gene knockdown mice were significantly lower compared to the control group. These findings indicate that Nagk regulates the expression of Gnrh and Kiss1 mRNA in GT1-7 cells, affects hypothalamus Gnrh mRNA levels and serum E2 concentration, and that its knockdown can delay puberty onset in female mice.

Crip Time Travels Through the Membrane and Vortex: An Autoethnographic Inquiry of Neurodivergent Student Temporality in Higher Art Education

AbstractCrip time is a fluid term with various definitions that pertain to the ways that disabled people experience time. In one sense, the effects of crip time can be constraining, particularly when it results in an encounter with ableist institutional and societal barriers. But crip time can also take on a liberatory form as a mode of resistance and a catalyst for structural change. This autoethnographic inquiry explores these various manifestations of crip time and will take form as a kind of crip time travel endeavour that recounts my experiences of temporality as a neurodivergent university art student. Framed through the mental imagery of the membrane and the vortex, I discuss the ways in which my neurodivergent student temporality collided and conflicted with the rigid temporal frameworks of neoliberal higher art education (HAE). I particularly focus on how HAE segments its programming into academic and artistic curricular time. I detail my difficulties keeping up with the academic curricular time to such an extent that the studio time and community time of artistic curricular time became lost or displaced time. Based on this crip time travel inquiry, I will acknowledge and move beyond a confining conception of crip time to offer insights into the liberatory potential of crip time towards reimagining temporal relations, reconceiving student success and opening time for neurodivergent students for critical making and thinking among a community of artist peers and mentors in HAE.

Interlayer bonding performance of 3D printed engineered cementitious composites (ECC): Rheological regulation and fiber hybridization

The weak interlayer adhesion caused by the layer-by-layer 3D printing (3DP) process and the incorporation of organic fiber in Engineered Cementitious Composites (ECC), detrimentally impacts the integrity of 3DP-ECC structures, particularly for large-scale structures requiring extended open time. To optimize the printing quality and extent the operation time, cellulose filaments (CF) were employed as nano-reinforcement, viscosity modifier and water retainer, and were hybridized with polyethylene fiber (PE) and steel fiber (ST). The highest bonding strength was raised up to 3.51 MPa. The time-dependent escalation of rheological parameters was mitigated, reducing interlayer porosity to 0.56 % and limiting the reduction in bonding strength to 12.01 % within 60 min open time. The compressive anisotropy was almost eliminated, verifying the potential of CF in modifying interlayer adhesion. A linear correlation between rheological behavior and interlayer bonding performance was established, and a 0.508 Pa s/min plastic viscosity growth rate was suggested to avoid cold joint and ensure printing quality.

Conservation of the Charge in Signal from Drift Tube Ion Mobility Spectrometers.

Quantitative information obtained from drift tube detectors used in ion mobility spectrometry is contained in the area of peaks forming the drift time spectrum. The area of all peaks corresponds to the total charge of ions entering the drift section of the spectrometer. It was found that this charge is not conserved when the ion composition changes. This work is devoted to studying the causes of this phenomenon. Experimental research consisted of recording drift time spectra for 2-pentanone and n-heptanone, at various analyte concentrations and different opening times of the shutter grid. Measurements of the total ion current were also performed in static mode with an open grid. The research results indicated that the reasons for the lack of ion charge conservation in the drift time spectrum are ion recombination, mutual repulsion, and mobility-dependent transmission of ions through the shutter grid. The explanation of the relationships obtained experimentally was based on a simple theoretical model, which considered the phenomenon of ion transport along the reaction section and the penetration of ions through the shutter. The developed model provides a good description of the measurement results and allows the estimation of ion currents and ion concentrations in the reaction section upstream of the grid. This information is important for proper quantitative analysis as well as when the ion mobility spectrometer is used in quantitative studies of chemical ionization processes.

Acupoint catgut embedding: a potential intervention strategy for obesity-related precocious puberty.

Obesity-related precocious puberty is induced by obesity, and acupoint catgut embedding (ACE) therapy is known to treat obesity. This study aims to validate the hypothesis that ACE can delay the onset of obesity-related precocious puberty. Female Sprague-Dawley rats, 21 days old, were randomly divided into three groups: the high-fat diet combined with ACE treatment group (ACE), the high-fat diet group (HFD), and the normal control diet group (NCD), with 8 rats in each group. The vaginal opening (VO) time was monitored, and serum levels of luteinizing hormone (LH), follicle-stimulating hormone (FSH), and total estradiol (E2) were measured, followed by statistical analysis. Kaplan-Meier survival curves, with VO as the endpoint, showed that vaginal opening was delayed in the ACE group compared to the HFD group, with a statistically significant difference (p < 0.05). The changes in levels of FSH, LH, and E2 indicated that sexual development was delayed in the ACE group compared to the HFD group and was more similar to the NCD group. Combining the vaginal opening time and changes in hormone levels, this study confirms the potential role of ACE in delaying the onset of obesity-related precocious puberty.

Methodology for Testing Selected Parameters of Low-Current Vacuum Electric Arc

This article presents the author’s methodology for testing selected parameters of a low-current vacuum arc, implemented using an innovative test stand based on a vacuum discharge chamber with a contact system mounted inside. In order to verify the validity of the adopted research methodology, as well as the correctness of the operation of the developed laboratory bench, measurements and calculations were made, among other things, of the energy and burning time of the vacuum arc, depending on selected factors, such as pressure and the delay time of the contact opening, calculated from the “passage through zero” of the sinusoid of the current flowing through the system. The tests were performed at 230 V and a current of 5 A for two pressure values: p1 = 1.00 × 105 Pa (atmospheric pressure) and p2 = 4.00 × 10−3 Pa (high vacuum environment). It was found that the vacuum insulation technique allows a significant reduction in the value of the arc energy and the burning time of the arc. It was also observed that in the case of a high vacuum environment, the ignition of the vacuum arc occurs after a time equal to about 3 ms from the “passage through zero” of the current flowing through the system. Below this value, the phenomenon did not occur. The results obtained provide an opportunity for the design and manufacturing of vacuum switchgear, where there is the prospect of reducing the negative effects associated with the arc burning process in the contact gap.

7849 The EDC Mixture “NeuroMix” Has Developmental, Anxiolytic, And Obesogenic Effects In Rats

Abstract Disclosure: E. Morales-Grahl: None. L. M. Thompson: None. E.N. Hilz: None. A.C. Gore: None. Humans are exposed to a variety of endocrine disrupting chemicals (EDCs) on a daily basis through drinking water, food, and contact with everyday objects. Safety guidelines aim to keep exposure to individual EDCs below certain levels but don’t take into account the potentially unpredictable effects of simultaneous exposure to multiple EDCs. Furthermore, EDCs may exhibit non-monotonic dose responses. The present study assessed the effect of an EDC mixture at three different doses on two sets of behaviors implicated in EDC research: anxiety, and high fat and sugar consumption. Beginning in early pregnancy and until pups were weaned at day 21, Sprague Dawley rats were fed daily with NeuroMix, our lab’s EDC mixture containing 9 common industrial chemicals, at three doses (0.1x, 1x, 10x). The base dose (1x) contained EDCs below the no observed adverse effect level in humans (NOAEL). In adulthood, the female and male offspring were then subjected to an open field and light/dark test, followed by a high fat and high sucrose preference test. 0.1x NeuroMix exposure advanced the timing of vaginal opening in female offspring, while all doses increased anogenital distance in males, marking differences in sexual development. 1x NeuroMix exposure in male rats increased their time in the open field corners and their time immobile in the corners, indicating higher anxiety. No difference was seen in sucrose preference. Female rats exposed to 1x NeuroMix showed a higher preference for a high-fat diet, and gained more weight compared to the controls while on this diet. These findings demonstrate that a mixture of human-relevant chemicals, when administered perinatally to rats, have developmental, anxiogenic and obesogenic effects in a sexually dimorphic manner. We also show a non-monotonic response to our chemical mixture, with the 1x dose showing greater effects than the 10x dose. Overall, we provide evidence that even at levels considered safe for individual EDCs, there are adverse developmental and behavioral effects of EDCs when combined. Grant support: R35 ES035024, F32 ES034257. Presentation: 6/1/2024

Norharmane potentiated anxiolytic- and antidepressant-like responses induced by imipramine and citalopram: an isobologram analysis.

β-carboline compounds display a therapeutic property for treating depression and anxiety behaviors. Imipramine and citalopram play an important role in the modulation of anxiety and depression behaviors. We investigated the effects of norharmane, imipramine, and citalopram on anxiety- and depression-like effects and their interactions. Elevated plus maze and forced swimming test were used for the assessment of anxiety- and depression-like behaviors in male mice. The results revealed that intraperitoneal (i.p.) administration of norharmane (10 mg/kg) increased percentage of open arm time (%OAT) in the elevated plus maze test and decreased immobility time in the forced swimming test, proposing anxiolytic- and antidepressant-like effects. Injection of imipramine (5 mg/kg; i.p.) enhanced %OAT and decreased immobility time, suggesting anxiolytic- and antidepressant-like effects. Moreover, norharmane potentiated the anxiolytic- and antidepressant-like responses induced by imipramine by increasing %OAT and decreasing immobility time. The results revealed additive anxiolytic- and antidepressant-like effects between norharmane and imipramine in mice. Alone, the administration of citalopram (5 mg/kg; i.p.) enhanced %OAT and reduced immobility time, causing anxiolytic- and antidepressant-like effects. When citalopram and norharmane were coinjected, norharmane augmented the anxiolytic- and antidepressant-like effects induced by citalopram by increasing %OAT and reducing immobility time. These results indicated additive anxiolytic- and antidepressant-like effects between norharmane and antidepressant drugs such as imipramine and citalopram on the modulation of anxiety and depression processes in mice.

A Randomized Controlled Study Comparing And Evaluating The Peri-Operative Outcomes Of Using Straight Versus Angled Handpieces During The Surgical Extraction Of Impacted Mandibular Third Molars

Introduction: The straight handpiece is one of the most commonly used instruments in third molar surgeries. However, its use has been suggested to carry a potential risk of soft tissue injury, possibly due to its design limitations leading to direct positioning and excessive heating, which could contribute to soft tissue damage. Direct positioning can sometimes cause excessive heating, leading to potential soft tissue damage. Additionall its design might limit accessibility in the confined surgical area, making it more difficult to maneuver effectively. This study aims to evaluate whether using an angled handpiece enhances accessibility and reduces the risk of soft tissue damage during surgical procedures. AIM: Comparison and evaluation of the use of straight and angled handpieces on perioperative outcomes in operative removal of obstructed lower third molars. Materials and Methods: This research included 30 healthy participants aged 18 to 40, all of whom were undergoing extraction of impacted lower third molars at the Department of Oral and Maxillofacial Surgery. Participants were randomly assigned to two groups.: Group A, where the extraction was carried out using an angled handpiece, and Group B, where a straight handpiece was employed. Results: No statistically significant differences were found in postoperative pain, edema, mouth opening, or surgical time between the angled and straight handpiece groups. Conclusion: The findings of our research indicate that there were no notable statistical differences observed between the two groups in relation to post-operative outcomes such as pain, edema, mouth opening, or surgical time. The lack of statistical significance may be attributed to the small sample size, emphasizing the importance of larger cohorts for a more accurate assessment of the clinical impact. However, operator comfort was reported to be better with angled handpieces in certain cases. Category: Dentistry, Pain Management, Patient Care

Novel use of clinical pathways to improve clinical trial accrual.

414 Background: Fewer than 10% of adult cancer patients participate in clinical trials. Clinical pathways platforms have traditionally sought to improve trial accrual by displaying available trials for eligible patients. Our team explored how Pathways navigation data can also be used to identify eligible patients of poorly accruing trials and provide study investigators with a near real-time data feed of those patients. Methods: Providers at Dana-Farber Cancer Institute are asked to navigate the pathways decision-support platform and order systemic cancer therapies 7 days in advance of administration to assist with authorization. We invited Principal Investigators (PIs) of poorly accruing trials to collaborate with our Pathways data team through our Pathways to Accrual to Clinical Trials (PACT) service. Through PACT, PIs are informed of patients who were navigated to nodes involving the trial in question but received standard therapy instead. PIs are given access to a Tableau Dashboard that is updated with new data daily and receive weekly reminders about the dashboard. The current analysis includes two trials that have been using PACT for at least one year. We reviewed monthly trial accrual rates from the time of the trials opening until March 2024. The dataset did not meet criteria for Poisson analysis, so Fischer’s exact test was used to compare accrual rates. We ensured the denominator of potentially eligible patients was relatively consistent. Results: DF-21-519 accrued 16 patients over 14 months prior to PACT and 33 patients over 12 months using PACT. DF-22-028 accrued 0 patients over 6 months prior to PACT and 12 patients over 14 months using PACT. Collectively, use of PACT more than doubled accrual rates for the involved trials (one-tailed p = 0.048). Conclusions: A real-time Pathways data feed can be used to identify patients for appropriate clinical resources. Here, we show how our PACT program helped to more than double clinical trial accrual for the involved studies. Scaling this technology across a broader swath of clinical trials could be an important advance for trial participation for users of this pathways platform. Trial Accruals, Pre PACT Accrual Rate, Pre PACT (Accruals/month) Accruals, Post PACT Accrual Rate, Post PACT (Accruals/month) DF-21-519 16 accruals in 14 mo 1.14 33 accruals in 12 mo 2.75 DF-22-028 0 accruals in 6 mo 0 12 accruals in 14 mo 0.86 Total 16 accruals in 20 mo 0.8 45 accruals in 26 mo 1.73 one-tailed p is 0.048

Chronic pregabalin treatment reduced anxiety, and acute pregabalin treatment increased depression-like behaviors in rats

BackgroundPregabalin is an antiepileptic drug that binds to the alpha-2/delta unit at presynaptic voltage-dependent calcium channels. We aimed to investigate the effect of acute and chronic pregabalin administration on anxiety and depression-like behaviors.MethodsFifty-six male Wistar albino rats were divided into seven groups: control, vehicle, and five different dose groups (5, 10, 30, 60, and 100 mg/kg). Pregabalin was administered for two weeks. Depression-like behaviors were evaluated by Forced swimming test. Anxiety-like behavior (ALB) was evaluated by Open field test (OFT), Elevated Plus Maze (EPM), and light-dark box. Subjects underwent the forced swimming test (FST) after the first dose, while the open field test (OFT), elevated plus maze (EPM), and light-dark box (LDB) were performed after two weeks of treatment. Further sucrose preference test was conducted to evaluate anhedonia until the end of the experiment.ResultsIn the forced swimming test, depression-like behaviors increased after acute single-dose administration of 10, 30, 60, 100 mg/kg pregabalin. According to OFT results, chronic 100 mg/kg pregabalin showed anxiolytic effects by decreasing grooming, and freezing behaviors. In addition, 100 mg/kg chronic pregabalin administration significantly increased the time spent in the central region, the number of entries to the center, and the unsupported rearing number without causing any change in locomotor activity. According to EPM results, both chronic 60 and 100 mg/kg pregabalin treatments showed anxiolytic effects by increasing open arm time and head dipping behavior. In addition, 60 and 100 mg/kg chronic pregabalin administration significantly decreased stretch attend posture. All pregabalin administrations between 5 and 100 mg/kg displayed anxiolytic effects in the LDB. Sucrose preference was above 65% for the duration of all experiments and subjects did not show anhedonia.ConclusionAcute pregabalin treatment triggered depression-like behaviors. Anhedonia, which may be associated with depression, was not observed during chronic treatment. Moreover, chronic treatment with pregabalin revealed potent anxiolytic effects in different behavior patterns and doses for all tests of unconditional anxiety. In particular, 100 mg/kg chronic pregabalin administration decreased anxiety-like behaviors in all experiment setups. Although the anxiolytic effect was demonstrated in chronic treatment, acute treatment of pregabalin induced depression-like behaviors, and thus in clinical practice should be done with caution, especially in patients with anxiety-depression comorbidity.

The Antidiabetic Drug Metformin Attenuated Depressive and Anxietylike Behaviors and Oxidative Stress in the Brain in a Rodent Model of Inflammation Induced by Lipopolysaccharide in Male Rats.

Inflammation is considered to be a link between diabetes and central nervous system (CNS) disorders, including depression and anxiety. Metformin is suggested to have antioxidant, anti-inflammatory, and mood-improving effects. The aim of the current research was to investigate the effects of the antidiabetic drug metformin on depressive- and anxiety- like behaviors and oxidative stress in the brain in a rodent model of inflammation induced by lipopolysaccharide (LPS) in male rats. The rats were treated as follows: (1) Vehicle instead of metformin and lipopolysaccharide, (2) Lipopolysaccharide (1 mg/ kg) + vehicle instead of metformin, (3-5) Lipopolysaccharide + 50, 100, or 150 mg/ kg of metformin. After the behavioral tests, including open field (OF), elevated pulse maze (EPM), and force swimming (FS) tests, the brains were removed, and malondialdehyde (MDA), nitric oxide (NO) metabolites, total thiol, catalase (CAT) activity, interleukin-6 (IL-6) and superoxide dismutase (SOD) activity were determined. In the EPM, metformin increased the open arm time and entry and decreased closed arm time and entry. In the FS test, metformin lowered the immobility and increased active time compared to lipopolysaccharide. In the OF test, metformin increased total crossing and total distance, time spent, traveled distance, and crossing number in the central zone. As a result of metformin administration, IL-6, MDA, and NO metabolites were decreased while thiol content, SOD, and CAT activity were increased. The results indicated that the well-known antidiabetic drug metformin attenuated depressive- and anxiety-like behaviors induced by inflammation in rats. These beneficial effects are suggested to be due to their attenuating effects on neuroinflammation, oxidative stress, and NO in the brain.

Early versus Delayed Cholecystectomy in Mild Gallstone Pancreatitis (Prospective Comparative Study)

Abstract Background Gallstone pancreatitis is the most common etiology of acute pancreatitis accounting for up to sixty percent of all cases. In patients with mild gallstone pancreatitis – characterized by the absence of organ failure, peripancreatic fluid collections or necrosis, and typical resolution within one week – cholecystectomy is the definitive treatment. Aim of the Work To determine the feasibility of early cholecystectomy during index admission as long as the symptoms and laboratory values are improving for patients with gallstone pancreatitis predicted to be mild on admission and to obtain unbiased estimates of the effect of early cholecystectomy on hospital LOS, complications, and patient-reported outcomes (PROs) in order to determine the need for further evaluation. Patients and Methods This Prospective comparative study was conducted at tertiary care hospitals at Ain Shams University hospitals and Nasser institute for medical research and treatment from May 2022 till December 2022 and performed on a total of 20 adult patients who aged 18 years old and over with mild pancreatitis and without cholangitis. Results Patients treated with early or late procedure experienced approximately the same intra operative bleeding (30 vs 50%), conversion to open (10 vs 10%), biliary injury (0 vs 0%), and operation time (50 vs 50 minutes) with no statistically significant differences between them (p value= 1.00, 0.65, 0.912) respectively. Conclusion Early cholecystectomy after gallstone pancreatitis is more advantageous than late cholecystectomy after gallstone pancreatitis because it is associated with less rate of recurrence of biliary pancreatitis, less complication of pancreatitis, less perioperative complication (adhesions, blood loss, biliary events, infection, postoperative pain) and shorter duration of postoperative hospital stay.

Designing the regularity of biodegradable copolyester for sustainable hot-melt adhesives: Adhesion, removability, and biodegradability

Recent research has increased in eco-friendly hot-melt adhesives as alternatives to conventional commercial hot-melt adhesives. However, there have been limitations in terms of removability and biodegradability. This study addresses these issues by designing a novel molecular structure for a single polymer, resulting in a sustainable hot-melt adhesive that offers strong adhesion, clear removability, and high biodegradability without additional additives. By varying the ratio of alcohol monomers 1,4-butanediol (BD) and ethylene glycol (EG) during polymerization, we synthesized poly(butylene adipate-co-butylene terephthalate-co-ethylene adipate-co-ethylene terephthalate) (PBEAT) with four block segments. We increased the open time by reducing the regularity of the molecular structure, controlling crystallization behavior, and inhibiting polymer chain packing. This improvement in wettability with the adherend allowed us to achieve a lap shear adhesion strength of 3.18 MPa. Additionally, we proposed a debonding mechanism based on the correlation between the crystallization temperature (Tc) block copolymer's and shear adhesion failure temperature (SAFT), demonstrating the removability of the prepared hot-melt adhesive. Finally, analyses of hydrolysis, enzymatic degradation, and biodegradation in compost confirmed that reduced crystallinity enhances biodegradability. PBE30AT, exhibiting the strongest adhesion strength, achieves complete degradation in compost within 20 days, faster than neat poly(butylene adipate-co-terephthalate) (PBAT). This research offers a novel and practical approach to enhancing the potential and expandability of sustainable adhesives by tailoring the molecular structure of the polymer.

Density-profile imaging using a second harmonic dispersion interferometer configured with reflective-beam expanders.

A Second-Harmonic Dispersion Interferometer (SHDI) is assembled to measure the two-dimensional, line-integrated density profile of a pulsed-plasma jet using probe-beam diameters well beyond the 1mm diameters typically used in such instruments. An initial prototype demonstrated the technique using 7mm beam diameters, which are now increased to 35mm diameter using two types of beam expanders: an achromatic-beam expander (ABE) or a reflective-beam expander (RBE). ABEs were found to add a periodic background to the measured-phase image with a magnitude of the order of Δϕ ∼ 2π radians, compared to the background phase noise level in the system configured without beam expanders at Δϕbg ∼ 0.025 radians. Subtraction of the background phase in a sample image reduced the effect of the ABE phase offset to a level of Δϕ ∼ 0.1 radians, enhancing the quality of the density measurements. Reflective-beam expanders (RBEs) did not modify the background phase appreciably and were a significant improvement, allowing the instrument to be used for 2D (r, z) density-profile measurements of a 3cm diameter × 5cm long, pulsed-plasma jet. Variations in the timing parameters for the plasma gun, specifically the gas valve opening time and the gas-injection delay, for a constant discharge current were used to map the plasma gun's performance, indicating a nominal line-integrated electron density of Nedl ≳ 3 × 1015 cm-2 and a volumetric density of Ne ≃ 9 × 1015cm-3. The results obtained for the RBE configuration demonstrate that the 2D-SHDI platform may be scaled to even larger sample areas and a rep-rated system, with the ability to potentially provide for a reproducible, time-resolved (∼1 ns), high resolution (∼100μm) measurement of 2D plasma-density profiles.

443P Assessing inter and intra-rater reliability of video hand opening time in myotonic dystrophy

443P Assessing inter and intra-rater reliability of video hand opening time in myotonic dystrophy

Validation of MLC leaf open time calculation methods for PSQA in adaptive radiotherapy with tomotherapy units.

Treatment delivery safety and accuracy are essential to control the disease and protect healthy tissues in radiation therapy. For usual treatment, a phantom-based patient specific quality assurance (PSQA) is performed to verify the delivery prior to the treatment. The emergence of adaptive radiation therapy (ART) adds new complexities to PSQA. In fact, organ at risks and target volume re-contouring as well as plan re-optimization and treatment delivery are performed with the patient immobilized on the treatment couch, making phantom-based pretreatment PSQA impractical. In this case, phantomless PSQA tools based on multileaf collimator (MLC) leaf open times (LOTs) verifications provide alternative approaches for the Radixact® treatment units. However, their validity is compromised by the lack of independent and reliable methods for calculating the LOT performed by the MLC during deliveries. To provide independent and reliable methods of LOT calculation for the Radixact® treatment units. Two methods for calculating the LOTs performed by the MLC during deliveries have been implemented. The first method uses the signal recorded by the build-in detector and the second method uses the signal recorded by optical sensors mounted on the MLC. To calibrate the methods to the ground truth, in-phantom ionization chamber LOT measurements have been conducted on a Radixact® treatment unit. The methods were validated by comparing LOT calculations with in-phantom ionization chamber LOT measurements performed on two Radixact® treatment units. The study shows a good agreement between the two LOT calculation methods and the in-phantom ionization chamber measurements. There are no notable differences between the two methods and the same results were observed on the different treatment units. The two implemented methods have the potential to be part of a PSQA solution for ART in tomotherapy.

Identification of African rice genotypes with early morning flowering trait to avoid heat stress

AbstractEarly morning flowering (EMF) is a desirable trait in rice to avoid heat stress as temperatures in early morning hours are low compared to afternoon and flowering is the most sensitive stage to heat exposure. Cultivated rice accessions including both Oryza glaberrima and O. sativa were phenotyped for an EMF trait in 2016–2018 in two locations viz. Cotonou in Benin Republic and Ibadan in Nigeria. The initial screening was done in Cotonou in three phases in 2016 dry season, 2017 wet season and 2017 dry season, respectively. The 2093 accessions used in initial screening were subdivided into three groups based on flowering duration ensuring that each accession was grown under conditions which were best suited to their flowering duration. Further screening was done in Ibadan in 2018 dry season and 2018 wet season. Out of 2093 accessions taken in the initial screening only 1754 accessions germinated out of which only 64 accessions exhibited EMF phenotype consistently across the cultivated years, seasons and locations. Among the 64 accessions exhibiting EMF phenotype, 15 accessions also showed an early peak in spikelet opening time (EPSOT) trait, more than 80% flowering before 09:00 h. We conclude that 15 O. glaberrima accessions identified possessing both the EMF and EPSOT traits are suitable donors for use in breeding for heat escape in rice.