Open Ecosystem Research Articles

Comparison of dimensionality reduction methods on hyperspectral images for the identification of heathlands and mires.

Hyperspectral data and machine learning offer great potential for identifying valuable open ecosystems. Due to the large volume of data, preprocessing of hyperspectral images must involve dimensionality reduction. The main goal of this study was to test the effectiveness of various types of feature reduction (feature selection and feature extraction) when performing classification using the Random Forest algorithm. A comparison was conducted between two ecosystems - heathlands and mires protected as Natura 2000 habitats. Two transformations of feature extraction were chosen, namely Minimum Noise Fraction (MNF) and Principal Component Analysis (PCA), while Linear Discriminant Analysis (LDA) was used as a feature selection method. It was proven that irrespective of the class type, accuracy is higher with the feature extraction method (mean F1 accuracy of 0.922) than with feature selection (mean F1 accuracy of 0.787). At the same time, no significant differences in accuracies were found between the MNF and PCA methods. Although LDA resulted in lower accuracies (0.816 for heathland and 0.750 for mires), the method could also be used due to relatively high F1 values. The effectiveness of the LDA method for feature reduction in open ecosystem identification was confirmed for the first time for open natural vegetation.

Open ecosystems restoration: a global review shows biases and mismatches between theory and practice

Ecological restoration aims to recover the structure, biodiversity, and function of degraded ecosystems. However, open ecosystems have been largely overlooked in restoration, and the extent of theory‐based restoration is unclear. We systematically review papers on open ecosystems restoration from 2011 to 2021, examining publication trends with data on restored site age, size, indicators, and assessment frequency. We also explore the relationships between interventions, indicators, and ecological concepts. By analyzing 343 papers, we found that open ecosystems restoration is concentrated in North America and Europe, with nearly 75% of restored areas originating in these continents. Restored areas are small, assessed for a short duration, with a plethora of indicators and no establishment of clear control or reference areas for meaningful comparisons. Common open ecosystems restoration sites interventions involved trees and shrubs management, soil management, seeding, and transfer of plant material as the main interventions. From our analyzed papers, we found that 30% of them mentioned any ecological concepts, with community assembly and succession being the most mentioned ones. Furthermore, our exploratory analysis revealed no relationship between interventions, indicators, and ecological concepts, suggesting a widespread mismatch between theory and practice. As the Decade for Ecosystem Restoration unfolds, we call practitioners, researchers, and stakeholders working on open ecosystems to address challenges and curtail widespread biases. Despite the uniqueness of each restoration project, a clear, well‐planned design encompassing site‐specific variables and solid theoretical bases is expected to enhance restoration outcomes, optimizing costs and biodiversity gains.

Neuronal wiring diagram of an adult brain

Connections between neurons can be mapped by acquiring and analysing electron microscopic brain images. In recent years, this approach has been applied to chunks of brains to reconstruct local connectivity maps that are highly informative1–6, but nevertheless inadequate for understanding brain function more globally. Here we present a neuronal wiring diagram of a whole brain containing 5 × 107 chemical synapses7 between 139,255 neurons reconstructed from an adult female Drosophila melanogaster8,9. The resource also incorporates annotations of cell classes and types, nerves, hemilineages and predictions of neurotransmitter identities10–12. Data products are available for download, programmatic access and interactive browsing and have been made interoperable with other fly data resources. We derive a projectome—a map of projections between regions—from the connectome and report on tracing of synaptic pathways and the analysis of information flow from inputs (sensory and ascending neurons) to outputs (motor, endocrine and descending neurons) across both hemispheres and between the central brain and the optic lobes. Tracing from a subset of photoreceptors to descending motor pathways illustrates how structure can uncover putative circuit mechanisms underlying sensorimotor behaviours. The technologies and open ecosystem reported here set the stage for future large-scale connectome projects in other species.

Distribution and Biology of Protaetia fieberi (Coleoptera, Scarabaeidae)-Is Protection Status Required?

Studies on saproxylic species of Coleoptera have garnered significant attention due to the rarity of some of them. To investigate the distribution and biology of Protaetia fieberi (Kraatz, 1880) (Scarabaeidae: Cetoniinae) in European Russia, we analyzed data from 16 regions collected between 2018 and 2024. This species has been reliably recorded in 26 regions. We describe the species' distribution area boundaries and discuss limiting factors that inhibit its spread to the north, south, and southeast of European Russia. The primary limiting factor is the lack of suitable biotopes. Within its distribution, Protaetia fieberi prefers deciduous and mixed forests of various types. These habitats include both old-growth forest ecosystems and secondary forests that have regenerated following logging. The species also inhabits man-made forest ecosystems, such as field protection forest belts, old parks, and gardens. In forest ecosystems, Protaetia fieberi tends to occupy the upper levels and is rarely found on the ground layer. Conversely, in open areas such as glades, the species is more commonly found at ground level. This distribution pattern is linked to the adults' feeding preferences, which include consuming sap on tree trunks in forests and feeding on flowering plants in open ecosystems. The seasonal activity of Protaetia fieberi peaks from the third decade of June to the second decade of July. It is hypothesized that the perceived rarity of Protaetia fieberi in research samples is due to the specific baiting methods used, with beer traps being the most effective. The status of the species is re-evaluated in light of new data, suggesting that Protaetia fieberi is common rather than rare in European Russia.

Ecology of Some Panorpa (Mecoptera, Panorpidae) Species from Several Regions of Russia

Our study focuses on the investigation of the ecological aspects (seasonal dynamics, height distribution, and preference of biotopes) of six species within the genus Panorpa (P. alpina Rambur, 1842, P. cognata Rambur, 1842, P. communis Linnaeus, 1758, P. germanica Linnaeus, 1758, P. hybrida MacLachlan, 1882, and P. vulgaris Imhoff & Labram, 1845). The observed seasonal dynamics predominantly display a monovoltine nature. P. communis was active from the early days of May, with individuals persisting until the beginning of October. Conversely, P. vulgaris exhibits activity from the third decade of May to mid-September. P. hybrida manifests within a concise timeframe, observed from late May to late July. P. cognata demonstrates activity commencing in early May, with individuals observed until the end of September. Within forest environments, the highest population density of all species is concentrated at a height of 1.5 m above ground level, whereas the minimum population density is recorded at a height of 12 m above ground level. P. vulgaris demonstrates comparable abundance across heights ranging from 1.5 to 7.5 m, whereas P. hybrida exhibits uniform distribution solely at heights of 1.5 and 3.5 m. Furthermore, the abundance of P. communis surpasses that at other heights when observed at the height of 1.5 m. Panorpa specimens exhibit an absence in open ecosystems at heights of 8 and 10 m. The peak of population density for all species is consistently identified at heights of 2 and 4 m. The application of six diverse entomological methodologies leads to optimal outcomes. Our investigations reveal that P. communis, P. vulgaris, and P. hybrida display greater attraction to beer traps, while P. cognata exhibits a comparatively diminished propensity for such traps. P. cognata, in contrast, demonstrates a substantial presence in pan traps and pitfall traps. In Malaise traps, pan traps, and pitfall traps, five distinct species were captured, although with a species composition differing from alternative methods. To sum up, for the comprehensive study of Panorpa across an expansive geographical spectrum, it is judicious to integrate both net captures and the use of diverse trap types. In addition, it is necessary to explore all biotopes and tiers of the forest.

The Interaction between Climate Change and Biodiversity Can Be Assessed from a Material Cycle Perspective

This study focused on understanding biodiversity variability in response to climate change as an environmental stressor from a carbon cycle perspective. As the frequency and magnitude of environmental stresses are expected to increase due to rising carbon concentrations, these changes may affect the variability in biodiversity. However, several studies have argued that conventional methods for biodiversity assessment, primarily based on arithmetic indices utilized in national policies, are inadequate for accurately assessing these interactions among species, abiotic changes, and material cycling. Here, we indicate that environmental stressors that occur at various scales and domains, from macroscopic to microscopic, and their effects on ecosystems are multi-dimensional. Furthermore, environmental stresses not only affect organisms inhabiting these ecosystems but these organisms, in return, influence the carbon cycle through life history changes resulting from these stresses. The results of our review suggest that these processes are sequentially interconnected, forming self-reinforcing feedback loops. Ultimately, an integrated approach is necessary to understand biodiversity variability in complex and open ecosystems. This approach should be capable of comprehending changes in the overall flow by considering ecosystem characteristics.

Macroinvertebrates as a Bioindicator of Water Quality in the Jangkok River, Lombok Island

Rivers are water bodies in open ecosystems where activities in the environment have an impact on river water quality. Monitoring of water quality is necessary to preserve aquatic resources. One of the river basins on Lombok Island is the Jangkok River. This river has a high utility, so water quality monitoring is needed. Macroinvertebrates are one of the best biological indicators for water quality monitoring. The objective of this study is to determine the diversity of macroinvertebrates and the quality of Jangkok River waters by using macroinvertebrates as bioindicators so that the results of this study can be used as a reference basis for the future use of Jangkok River water in the allocation of community needs. The method used in this research is biomonitoring with macroinvertebrate bioindicators, and the technique used in sampling is purposive sampling. Sampling was conducted at three stations, namely the upstream, middle, and downstream parts of the Jangkok River. The data analysis used was the Family Biotic Index (FBI) supported by physical-chemical parameters. The results of this study showed that the macroinvertebrates identified were 721 individuals consisting of 5 classes, 17 orders, and 36 families. The water quality of the Jangkok River, based on the FBI value in the upstream and middle sections, is still included in the good category, while in the downstream section, it falls into the very bad category.

Innovation and creativity in gastronomy beyond Haute Cuisine restaurants: Towards an innovation ecosystem in Gastronomytech in the Basque Country

The new avant‐garde of innovation in the field of gastronomy is moving from Haute Cuisine restaurants to laboratories, research centres, hubs or ecosystems fully dedicated to culinary innovation, where mixed and multidisciplinary teams of cooks and scientist work together. Such agents are leading an expansion of the field of influence of culinary innovation, connecting culinary knowledge with sectors such as agri‐food, hospitality, tourism, health or biotechnology. And, in turn, they seek to expand the frontiers of culinary innovation by exploring the connection of gastronomy with new digital technologies, promoting gastronomic entrepreneurship or developing policies to boost advanced gastronomic ecosystems around the world. The article analyses this process through the case study of the emergence of an innovation ecosystem in Gastronomytech in the Basque Country (Spain) led by the agents BCC Innovation, LABe Digital Gastronomy Lab and GOe Gastronomy Open Ecosystem, all three managed by Basque Culinary Center (an organization where training, innovation, research and entrepreneurship coexist with the aim of developing and promoting gastronomy). The study has implications for the management of culinary creativity and innovation, both for chefs and innovation teams at restaurants, as well as for policy‐makers who aim to promote innovative gastronomy in the future.

Ten years of directing seeding restoration in the Brazilian savanna: Lessons learned and the way forward

Savannas and grasslands have lost almost 50% of their original cover worldwide. Therefore, the development of methods and information on open-canopy ecosystem restoration is urgent for the inclusion of these ecosystems into global and regional priorities. In the Brazilian savanna, the most diverse savanna in the world, restoration efforts focused on open ecosystems have been virtually absent, but have increased in the last 10 years. Such efforts are frequently threatened by invasive exotic grasses (IEG) that invade and dominate areas excluding native species, oftentimes aided by altered soil conditions. Long-term studies of savanna restoration trajectories are rare. In this study, we surveyed 22 savanna restoration areas established two to ten years before the study with similar restoration methods to assess their current status. We show that the current restoration methods are successful in establishing native species and allowing species turnover but they are threatened by IEG. Restoration success varies and is affected by soil conditions, IEG landscape cover and post-sowing weeding. Despite that, the simultaneous introduction of different plant functional groups allows turnover from fast to slow-growing plants. Establishing savanna native species is possible at an operational scale with current knowledge and techniques. However, native species establishment fails to prevent IEG reinfestation, which needs to be managed in restoration efforts in the Brazilian savanna.

Fire and grazing interaction in a subhumid grassland: Effects on a dominant tussock grass

AbstractQuestionsHerbivory and fire are the main disturbance drivers in open ecosystems. The aim of this study was to evaluate the effects of fire, cattle grazing, and their interaction on structural traits and the Normalized Difference Vegetation Index (NDVI) of the tussock grass Saccharum angustifolium, and its temporal dynamics in a subhumid grassland.LocationGrasslands of the Eastern Hills, Uruguay.MethodsWe set three blocks (four plots of 300 m2 each) in a homogeneous grazed area dominated by S. angustifolium within a protected area. Each block presented the combinations that emerged from the two levels of cattle grazing (grazed/ungrazed) and burning (burned/unburned) factors. The experimental burning was carried out in the spring. Clump volume, number of flowering culms, NDVI of S. angustifolium, and soil variables were measured for 2 years.ResultsBurning efficiency (plant biomass burned of the total available) was 82%, burn intensity ranged from 178–610 kW m−1, and the soil temperature increased 40% compared with pre‐fire measurements. The volume of S. angustifolium clumps decreased with fire, and the difference between burned–unburned plots decreased with time. The burned–ungrazed treatment increased the number of S. angustifolium flowering culms. NDVI of S. angustifolium clumps increased 50% with fire, and then decreased with time. Soil temperature was higher in burned treatments, and moisture was higher in unburned treatments on some dates. Bulk density, for the 0–5‐cm depth, was 9% higher in burned than in unburned plots irrespective of grazing.ConclusionsOur results provide new data in favor of the management practice carried out by ranchers in Uruguayan grasslands dominated by S. angustifolium. Our data complement the evidence that a fire–grazing interaction can maintain grasslands in different phases of a stable state. Our results constitute a useful contribution for the managers/authorities of the protected area, and can be extrapolated to other tussock grass‐dominated grassland areas.

Controlled Environment Ecosystem: A Cutting-Edge Technology in Speed Breeding.

The controlled environment ecosystem is a meticulously designed plant growing chamber utilized for cultivating biofortified crops and microgreens, addressing hidden hunger and malnutrition prevalent in the growing population. The integration of speed breeding within such controlled environments effectively eradicates morphological disruptions encountered in traditional breeding methods such as inbreeding depression, male sterility, self-incompatibility, embryo abortion, and other unsuccessful attempts. In contrast to the unpredictable climate conditions that often prolong breeding cycles to 10-15 years in traditional breeding and 4-5 years in transgenic breeding within open ecosystems, speed breeding techniques expedite the achievement of breeding objectives and F1-F6 generations within 2-3 years under controlled growing conditions. In comparison, traditional breeding may take 5-10 years for plant population line creation, 3-5 years for field trials, and 1-2 years for variety release. The effectiveness of speed breeding in trait improvement and population line development varies across different crops, requiring approximately 4 generations in rice and groundnut, 5 generations in soybean, pea, and oat, 6 generations in sorghum, Amaranthus sp., and subterranean clover, 6-7 generations in bread wheat, durum wheat, and chickpea, 7 generations in broad bean, 8 generations in lentil, and 10 generations in Arabidopsis thaliana annually within controlled environment ecosystems. Artificial intelligence leverages neural networks and algorithm models to screen phenotypic traits and assess their role in diverse crop species. Moreover, in controlled environment systems, mechanistic models combined with machine learning effectively regulate stable nutrient use efficiency, water use efficiency, photosynthetic assimilation product, metabolic use efficiency, climatic factors, greenhouse gas emissions, carbon sequestration, and carbon footprints. However, any negligence, even minor, in maintaining optimal photoperiodism, temperature, humidity, and controlling pests or diseases can lead to the deterioration of crop trials and speed breeding techniques within the controlled environment system. Further comparative studies are imperative to comprehend and justify the efficacy of climate management techniques in controlled environment ecosystems compared to natural environments, with or without soil.

Shedding light on the relevance of asexual reproduction in the savannas: Cloning in Velloziaceae

AbstractDuring a biodiversity monitoring of woody and herbaceous plants in 2023, we observed the formation of new individuals through cloning in Velloziaceae species. Based on recorded evidence and scientific literature, we discussed the importance of cloning in maintaining the high dominance of these species in seasonal open ecosystems. In addition, we hope to shed light on the discussion of the importance of asexual reproduction through cloning and resprouting strategies in plants widely widespread in savanna ecosystems.Abstract in Portuguese is available with online material.

Woody plant encroachment drives population declines in 20% of common open ecosystem bird species.

Grassy ecosystems cover more than 40% of the world's terrestrial surface, supporting crucial ecosystem services and unique biodiversity. These ecosystems have experienced major losses from conversion to agriculture with the remaining fragments threatened by global change. Woody plant encroachment, the increase in woody cover threatening grassy ecosystems, is a major global change symptom, shifting the composition, structure, and function of plant communities with concomitant effects on all biodiversity. To identify generalisable impacts of encroachment on biodiversity, we urgently need broad-scale studies on how species respond to woody cover change. Here, we make use of bird atlas, woody cover change data (between 2007 and 2016) and species traits, to assess: (1) population trends and woody cover responses using dynamic occupancy models; (2) how outcomes relate to habitat, diet and nesting traits; and (3) predictions of future occupancy trends, for 191 abundant, southern African bird species. We found that: (1) 63% (121) of species showed a decline in occupancy, with 18% (34) of species' declines correlated with increasing woody cover (i.e. losers). Only 2% (4) of species showed increasing population trends linked with increased woody cover (i.e. winners); (2) Open habitat specialist, invertivorous, ground nesting birds were the most frequent losers, however, we found no definitive evidence that the selected traits could predict outcomes; and (3) We predict open habitat loser species will take on average 52 years to experience 50% population declines with current rates of encroachment. Our results bring attention to concerning region-wide declining bird population trends and highlight woody plant encroachment as an important driver of bird population dynamics. Importantly, these findings should encourage improved management and restoration of our remaining grassy ecosystems. Furthermore, our findings show the importance of lands beyond protected areas for biodiversity, and the urgent need to mitigate the impacts of woody plant encroachment on bird biodiversity.

Restoration of open ecosystems in the face of climate change

Open ecosystems occur all around the world in various forms including temperate and tropical grasslands, savannas, shrublands, heathlands, among others. They are home to unique biodiversity, provide key ecosystem services and sustain traditional livelihoods of nearly two billion people. In the face of ongoing climate change, practitioners aiming to restore open ecosystems need the support of the scientific community more than ever. The aim of this Special Issue (SI) is to provide an attention-grabbing collection of high-quality publications addressing the growing challenges of open ecosystems restoration. The SI contains 14 papers that fill various, often interdisciplinary knowledge gaps. Three papers deal with the challenges of identifying the right target states, including the genetic composition of constituting plant species, for restoration under changing environmental conditions and competing stakeholder interests. Five papers advance our understanding on the appropriate timing and methodological toolkit to actively ignite re-assembly of the target plant communities, while two papers focus on situations where spontaneous processes can still also be relied on. The interaction of open ecosystems health and recovery with higher trophic levels, particularly grazers, is also discussed in three papers. Finally, a review paper systematically identifies further knowledge gaps, such as the role of soil microbes in grassland recovery and makes clear guidelines how to fill them. Due to the variety of topics and the rigorous content, this SI provides strong support for open ecosystems restoration policy and practice under the UN Decade on Ecosystem Restoration and beyond.

Global plant responses to intensified fire regimes

AbstractAimGlobal change factors, such as warming, heatwaves, droughts and land‐use changes, are intensifying fire regimes (defined here as increasing frequency or severity of fires) in many ecosystems worldwide. A large body of local‐scale research has shown that such intensified fire regimes can greatly impact on ecosystem structure and function through altering plant communities. Here, we aim to find general patterns of plant responses to intensified fire regimes across climates, habitats and fire regimes at the global scale.LocationWorldwide.Time periodStudies published 1962–2023.Major taxa studiedWoody plants, herbs and bryophytes.MethodsWe carried out a global systematic review and meta‐analysis of the response of plant abundance, diversity and fitness to increased fire frequency or severity. To assess the context dependency of those responses, we tested the effect of the following variables: fire regime component (fire frequency or severity), time since the last fire, fire type (wildfire or prescribed fire), historical fire regime type (surface or crown fire), plant life form (woody plant, herb or bryophyte), habitat type and climate.ResultsIntensified fire regimes reduced overall plant abundance (Hedges' d = −0.24), diversity (d = −0.27), and fitness (d = −0.69). Generally, adverse effects of intensified fire regimes on plants were stronger due to increased severity than frequency, in wildfires compared to prescribed fires, and at shorter times since fire. Adverse effects were also stronger for woody plants than for herbs, and in conifer and mixed forests than in open ecosystems (e.g. grasslands and shrublands).Main conclusionsIntensified fire regimes can substantially alter plant communities in many ecosystems worldwide. Plant responses are influenced by the specific fire regime component that is changing and by the biotic and abiotic conditions.

Pyric herbivory in a temperate European wood‐pasture system

Abstract The term pyric herbivory was first introduced in 2009, describing how fire shapes herbivory as burned areas attract herbivores and, simultaneously, herbivory shapes fuel load and fire behaviour. Pyric herbivory results in a mosaic of patches with varying levels of herbivory and grazing intensity fire intensity and frequency. The importance of pyric herbivory for ecosystem heterogeneity and biodiversity has been described for North American, Australian and African systems, but the concept remains largely untested in a European context. We introduced fire and herbivory in a full‐factorial experiment in a temperate European wood‐pasture system to test whether pyric herbivory operates in ways comparable to grassy systems elsewhere in the world. Using camera traps, we observed the behaviour of cattle in burned subplots (49 m2) compared with unburned subplots. We measured grass height and the proportion of the subplot that burned as variables affecting cattle preference and to assess how grazing affects fire behaviour. We also examined the effect on plant species and life‐form composition after six seasons of treatment. Cattle spent more time grazing in burned than in unburned subplots in the most productive paddock, where a larger proportion of the subplot burned. The proportion of a subplot that burned was positively related to pre‐fire grass height. Moreover, both grass height and the proportion of subplot burned declined in the burned subplots during the 6‐year study period and fire and cattle grazing altered the relative cover of graminoids and shrubs (Rubus spp.), with more graminoids in grazed and/or burned subplots and more shrubs in ungrazed subplots at the end of the study. Synthesis and applications. In our temperate European wood pasture, fire and (cattle) grazing interacted in ways comparable to pyric herbivory in grassy ecosystems elsewhere in the world, especially in the most productive paddock. Fire attracted grazing, with cattle grazing longer on subplots that burned more fully. Grazing also affected fire, where over the course of our experiment cattle grazing reduced grass height and the proportion of a subplot that burned. We suggest that pyric herbivory is an interesting management method to further explore in the European context to address the loss of biodiversity in open ecosystems, particularly in more productive sites.

An open-source FACS automation system for high-throughput cell biology.

Recent advances in gene editing are enabling the engineering of cells with an unprecedented level of scale. To capitalize on this opportunity, new methods are needed to accelerate the different steps required to manufacture and handle engineered cells. Here, we describe the development of an integrated software and hardware platform to automate Fluorescence-Activated Cell Sorting (FACS), a central step for the selection of cells displaying desired molecular attributes. Sorting large numbers of samples is laborious, and, to date, no automated system exists to sequentially manage FACS samples, likely owing to the need to tailor sorting conditions ("gating") to each individual sample. Our platform is built around a commercial instrument and integrates the handling and transfer of samples to and from the instrument, autonomous control of the instrument's software, and the algorithmic generation of sorting gates, resulting in walkaway functionality. Automation eliminates operator errors, standardizes gating conditions by eliminating operator-to-operator variations, and reduces hands-on labor by 93%. Moreover, our strategy for automating the operation of a commercial instrument control software in the absence of an Application Program Interface (API) exemplifies a universal solution for other instruments that lack an API. Our software and hardware designs are fully open-source and include step-by-step build documentation to contribute to a growing open ecosystem of tools for high-throughput cell biology.

Evaluating methods for measuring the leaf area index of encroaching shrubs in grasslands: From leaves to optical methods, 3-D scanning, and airborne observation

Leaf area index (LAI) is a key variable describing ecosystem structure and influencing the exchange of carbon, water, and energy. LAI is often evaluated with indirect methods. However, the accuracy of indirect measurements can vary with canopy structure and is not always generalizable across ecosystems. Previous research has characterized the accuracy of indirect methods for woody plants in forest ecosystems, but it is not well established for woody plants in open ecosystems—despite having large differences in canopy structure. In this study, we compared direct LAI measurements in clonal grassland shrub canopies to three indirect methods: a ceptometer, a handheld 3D scanner (processed using EXScanPro-3.6 software), and NEON's LAI product obtained from airborne hyperspectral imaging (derived from SAVI). To our knowledge, this is the first study to assess the accuracy of leaf area data in woody plants using a handheld 3D scanner and one of few studies assessing the accuracy of the National Ecological Observation Network's (NEON) Airborne Observation Platform—our source of airborne hyperspectral imaging. Data were collected in tallgrass prairie undergoing woody encroachment and three treatments: no herbivore disturbance, bison present, and simulated browsing. We found that direct LAI measurements of control and grazed C. drummondii canopies averaged ∼8.0. The ceptometer accurately estimated LAI in non-browsed canopies but overestimated LAI of browsed canopies by 38 %. One-sided leaf area of ramets measured with a handheld 3D scanner was strongly related to direct measurements (r2=0.86), but underestimated leaf area at greater values. LAI estimated from airborne spectral data underestimated LAI by 55 %. We conclude that a ceptometer was adequate for measuring LAI in dense shrub canopies when browsing was not present, the handheld 3D scanner was adequate for measuring leaf area of individual ramets, and the airborne spectral data was not suitable for estimating LAI of dense, grassland shrub canopies.

Envisioning Transition from Open Landscapes to Forested Landscapes in the Routt National Forest, Colorado, United States

Globally, in remaining wildlands, tree densities and forested cover have increased in grasslands and open forests since European settlement. In the southern Rocky Mountains of Colorado, United States, we determined tree composition and tree cover from historical (years 1875 to 1896) surveys and compared them to current (2002 to 2011) tree composition and current (year 2016) forested land cover for 500,000 ha of the Routt National Forest. Additionally, we examined whether changes in precipitation occurred. Regarding composition, pine (primarily lodgepole pine; Pinus contorta) decreased from 65% to 32% of all trees, with increased subalpine fir (Abies lasiocarpa) from 0.5% to 23% of all trees, and quaking aspen (Populus tremuloides) from 13% to 30% of all trees. According to 80% of 5175 survey points not in forests, the historical landscape was very open, comprised of grasslands, mountain meadows, and other open ecosystems. In contrast, 75% of the current landscape is covered by forests. Change points in the Palmer Modified Drought Index were within historical limits, indicating that forestation was not related to a change in water availability. Based on historical surveys and accounts, we envisioned a historical landscape that was open but embedded with dense lodgepole pine clusters and spruce stands at high elevations, which has now become a predominantly forested landscape of dense forests, similar to global forestation patterns.

Modern pollen rain reveals differences across forests, open and mosaic landscapes in Madagascar

Societal Impact StatementPalaeoecological investigations are crucial in understanding millennial to centennial land use and land cover change. By analysing the modern pollen rain from four main vegetation types across Madagascar, this research provides baselines for improving the interpretation of pollen records in palaeoecological studies. This study determines the modern pollen‐vegetation relationship in Madagascar and gives a better understanding of the island's landscapes. Despite some spatial limitations, this approach contributes to resolving the debated topic related to the origin of Madagascar's open ecosystems. Knowing the vegetation history prior to and after human settlement would help guide biodiversity management and its associated ecosystem services.Summary Modern pollen rain contributes to calibrating and defining palaeo‐records relative to the current vegetation of diverse landscapes. Quantifying past vegetation change is important to better frame sustainable and resilient management methods of biodiversity considering human needs. In this paper, we aim to assess modern pollen assemblages in different vegetation types in Madagascar and inform how these could contribute to the interpretation of palaeo‐records. We identified modern pollen assemblages from 21 samples across four major vegetation types in Madagascar, including seven (N = 7) newly generated samples from the tropical dry forest in the Northwest region and 14 datasets downloaded from the African Pollen Database website. The downloaded datasets are from core tops representing modern vegetation previously analysed in the tropical dry forests and spiny thickets, the wooded grassland–bushland mosaic and the littoral humid forest. We evaluated the frequency of different taxa in the samples and conducted multivariate analyses to evaluate the similarities and ecological affinities between samples. Pollen rain reflected the main vegetation types across Madagascar and provided information on the current structure and state of the landscape. Regarding the interpretation of pollen records, pollen rain samples revealed that: (i) Open mosaic ecosystems should contain a percentage of at least 40% grass pollen, informing on the origin of the vegetation in older samples and the state of degradation in more recent vegetation reconstructions; and (ii) Anthropogenic vegetation such as plantations are marked by the abundance of pioneer taxa such as Pinus and/or Eucalyptus spp. with a frequency >20%. Our findings improve the interpretations of pollen records and help differentiate past distribution of forest, open and mosaic vegetation, in Madagascar. It will contribute to further research on human practices, land use and into scenarios of conservation planning not only in Madagascar but also across the tropics.