Little is known about the diversity of Calanoida, Copepoda, in different habitats of the north-western Red Sea. In this study, biodiversity of Calanoida, Copepoda, during the cold and warm seasons of 2017, were observed at 12 stations belonging to four different habitats (coral reef (CR), sheltered shallow lagoons (SSL), seagrass (SG), and open deep-water (ODW) habitats) in the Hurghada shelf, north-western Red Sea. SSL habitats were the most affected by environmental conditions, especially temperature, salinity, and depth. Some calanoid species were restricted to certain habitats, others were adapted to live in more than one habitat, while some species showed a wide distribution in all habitats. ODW habitats showed maximum diversity and density of the calanoid species. The effects of temperature and salinity were distinct in the SG and SSL. The results clearly showed that different Red Sea habitats affected the biodiversity of calanoid copepods.