OpenACC Research Articles

Porting and Optimizing GTC-P on TaihuLight Supercomputer with OpenACC

Porting and Optimizing GTC-P on TaihuLight Supercomputer with OpenACC

Analysis of parallelisation of 3D-CEMBS model using technologies like OpenACC and OpenMP

Analysis of parallelisation of 3D-CEMBS model using technologies like OpenACC and OpenMP

1-MCP pretreatment prevents bud and flower abscission in Dendrobium orchids

Dendrobium orchid inflorescences were treated for 4 h at 25 °C with or without 100–500 nl/l 1-MCP and were then placed in water at 25 °C to follow abscission. In controls, depending on the experiment, 20–80% of the floral buds and 0–20% of the open flowers abscised within 1 week. The 1-MCP pretreatment largely prevented this abscission. If flowers were exposed to 1.0 μl/l ethylene for 3 days, all floral buds and all open flowers abscised within the 3 days of treatment. 1-MCP treatment just prior to ethylene treatment largely prevented the ethylene effect. Treatment with STS was as effective as treatment with 1-MCP. Dendrobium inflorescences are usually shipped by air in cardboard boxes lined with plastic film. The stem ends are placed in plastic tubes filled with water. After shipment and placement in water, a considerable percentage of the buds, and some flowers, abscise. This is probably due to elevated ethylene concentrations inside the boxes. Treatment of the inflorescences with 100–500 nl/l 1-MCP prior to simulated air transport largely prevented abscission during vase life. 1-MCP treatment inhibited ethylene production of the inflorescences by lowering both ACC synthase in open flowers and ACC oxidase activity in floral buds.

Optimizing Preparative LC/MS Configurations and Methods for Parallel Synthesis Purification

Preparative LC/MS is widely employed to address the high-throughput purification demands of parallel synthesis. However, conflicting chromatography requirements and the complexities of applying MS-directed fractionation can limit its effectiveness in high-throughput parallel synthesis schemes. We report here an at-column dilution small-scale preparative LC configuration which satisfies the mass loading (>20 mg) and small fraction volume (<1.5 mL) requirements of discovery parallel synthesis schemes employing plate mapping. We also present a protocol for compound-specific optimization of the preparative gradient LC method and MS threshold for fractionation from a “prepreparative” LC/MS analysis of the crude material. We will demonstrate significantly improved preparative separations (relative to “universal” prep LC methods) and the selection of reliable and effective fraction threshold methods for diverse libraries. The methods and configurations are simple to implement and are equally suited for “open acc...