OBJECTIVE: This study was performed to assess the survival, development, and pregnancy and delivery rates of cryopreserved embryos derived from sperm obtained from fresh- or frozen-thawed testicular tissue.DESIGN: Retrospective analyzed study from 2006 to 2009.MATERIALS AND METHODS: In fresh IVF cycles (n=70), ICSI was performed using sperm retrieved from fresh (TESE-Fresh IVF cycles; n=29) and frozen-thawed testicular tissue (t-TESE-Fresh IVF cycles; n=41) in patients with obstructive and non-obstructive azoosperma. Testicular tissues were cryopreserved by programmed cell freezer (Cryomagic I, Korea). The surplus or all fertilized embryos (zygotes, cleavaged embryos and blastocysts) in the fresh IVF cycles were cryopreserved by slow freezing or vitrification. Total 85 frozen-thawed ET cycles (Cryo ET) were divided as cryopreserved embryos derived from fresh (TESE-Cryo ET; n=35) group and frozen-thawed TESE (t-TESE-Cryo ET; n=50) group. Embryo survival, embryo grades, pregnancy and delivery rates were compared between two groups. Chi-square test and t-test were used for statistical analysis.RESULTS: There were no significant differences in clinical parameters of fresh cycles between TESE-Fresh IVF cycles and t-TESE-Fresh IVF cycles. In Cryo-ET cycles, rate of survival (95.7% vs. 94.0%) and good quality embryos (82.4%, vs. 71.9%) were similar between TESE-Cryo ET and t-TESE-Cryo ET. And pregnancy and implantation rates were also similar between two groups (54.3% vs. 52.5% and 28.4% vs. 24.4%). Delivery rates were slightly higher in t-TESE-Cryo ET (46.0%) than TESE-Cryo ET (30.3%). However, there were not significantly different.CONCLUSION: The pregnancy and delivery rates of frozen-thawed embryos derived from frozen-thawed TESE were obtained similar to those of derived from fresh TESE. Therefore, we can suggest that frozen-thawed embryos using frozen-thawed TESE could achieve a relevant clinical outcome in IVF cycles and avoids further oocyte retrieval and TESE procedures. OBJECTIVE: This study was performed to assess the survival, development, and pregnancy and delivery rates of cryopreserved embryos derived from sperm obtained from fresh- or frozen-thawed testicular tissue. DESIGN: Retrospective analyzed study from 2006 to 2009. MATERIALS AND METHODS: In fresh IVF cycles (n=70), ICSI was performed using sperm retrieved from fresh (TESE-Fresh IVF cycles; n=29) and frozen-thawed testicular tissue (t-TESE-Fresh IVF cycles; n=41) in patients with obstructive and non-obstructive azoosperma. Testicular tissues were cryopreserved by programmed cell freezer (Cryomagic I, Korea). The surplus or all fertilized embryos (zygotes, cleavaged embryos and blastocysts) in the fresh IVF cycles were cryopreserved by slow freezing or vitrification. Total 85 frozen-thawed ET cycles (Cryo ET) were divided as cryopreserved embryos derived from fresh (TESE-Cryo ET; n=35) group and frozen-thawed TESE (t-TESE-Cryo ET; n=50) group. Embryo survival, embryo grades, pregnancy and delivery rates were compared between two groups. Chi-square test and t-test were used for statistical analysis. RESULTS: There were no significant differences in clinical parameters of fresh cycles between TESE-Fresh IVF cycles and t-TESE-Fresh IVF cycles. In Cryo-ET cycles, rate of survival (95.7% vs. 94.0%) and good quality embryos (82.4%, vs. 71.9%) were similar between TESE-Cryo ET and t-TESE-Cryo ET. And pregnancy and implantation rates were also similar between two groups (54.3% vs. 52.5% and 28.4% vs. 24.4%). Delivery rates were slightly higher in t-TESE-Cryo ET (46.0%) than TESE-Cryo ET (30.3%). However, there were not significantly different. CONCLUSION: The pregnancy and delivery rates of frozen-thawed embryos derived from frozen-thawed TESE were obtained similar to those of derived from fresh TESE. Therefore, we can suggest that frozen-thawed embryos using frozen-thawed TESE could achieve a relevant clinical outcome in IVF cycles and avoids further oocyte retrieval and TESE procedures.