Onset Of Puberty In Gilts Research Articles

Ovary-derived circular RNAs profile analysis during the onset of puberty in gilts

BackgroundIn mammals, the ovary is the essential system of female reproduction for the onset of puberty, and the abnormal puberty has negative outcomes on health. CircRNA is a non-coding RNA produced by non-canonical alternative splicing (AS). Several studies have reported that circRNA is involved in the gene regulation and plays an important role in some human diseases. However, the contribution of circRNA has received little known within the onset of puberty in ovary.ResultsHere, the profiles of ovarian circRNAs across pre-, in- and post-pubertal stages were established by RNA-sEq. In total, 972 circRNAs were identified, including 631 stage-specific circRNAs and 8 tissue-specific circRNAs. The biological functions of parental genes of circRNAs were enriched in steroid biosynthesis, autophagy-animal, MAPK signaling pathway, progesterone-mediated oocyte maturation and ras signaling pathway. Moreover, 5 circRNAs derived from 4 puberty-related genes (ESR1, JAK2, NF1 and ARNT) were found in this study. The A3SS events were the most alternative splicing, but IR events were likely to be arose in post-pubertal ovaries. Besides, the circRNA-miRNA-gene networks were explored for 10 differentially expressed circRNAs. Furthermore, the head-to-tail exon as well as the expressions of 10 circRNAs were validated by the divergent RT-qPCR and sanger sequencing.ConclusionsIn summary, the profiles of ovarian circRNAs were provided during pubertal transition in gilts, and these results provided useful information for the investigation on the onset of puberty at the ovarian-circRNAs-level in mammals.

Genome-Wide DNA Methylation Analysis of Hypothalamus During the Onset of Puberty in Gilts

Although selection of the early age at puberty in gilts will make for a favorable effect on the reproductivity of sow, a large proportion of phenotypic variation in age at puberty of gilts cannot be explained by genetics. Previous studies have implicated hypothalamic DNA methylation in the onset of puberty in mammals. However, the underlying molecular mechanism regarding the regulation of the onset of puberty has remained largely unexplored in gilts. Herein, the genome-scale DNA methylation of hypothalamus was acquired, using the reduced representation bisulfite sequencing, to compare and describe the changes of DNA methylation across Pre-, In- and Post-pubertal gilts. In this study, the average methylation levels of CpGs and CpHs (where H = C, T, or A) in CpG islands- and gene-related regions were gradually decreased in hypothalamic methylomes during the pubertal transition. Comparisons of Pre- vs. In-, In- vs. Post-, and Pre- vs. Post-pubertal stage revealed that there were 85726, 92914, and 100421 differentially methylated CpGs and 5940, 14804, and 16893 differentially methylated CpHs (where H = C, T, or A) in the hypothalamic methylomes. The methylation changes of CpHs were more dynamic than that of CpGs, and methylation changes of CpGs and CpHs were likely to be, respectively, involved in the developmental processes of reproduction and the molecular processes of cellular communications in the hypothalamus. Moreover, methylation changes of CpHs were observed to overrepresent in the quantitative trait loci of age at puberty, and the biological function of these CpH methylation changes was enriched in the pancreas development in gilts. Furthermore, the mRNA levels of several differentially CpG or CpH methylated genes related to the transcription of RNA II polymerase, GnRH signaling pathway, Estrogen signaling pathway, PI3K-AKt signaling pathway, and Insulin signaling pathway, including MAX, MMP2, FGF11, IGF1R, FGF21, and GSK3B, were significantly changed across these pubertal stages in the hypothalamus. These results will help our understanding of how DNA methylation contributes to phenotypic variation of age at puberty.

PSIII-38 Effect of Deficiency or Surplus Dietary Energy Intake on the Onset of Puberty in Gilts Investigated by Liquid Chromatography-Mass Spectrometry Based Follicular Fluid Metabolomics Analysis.

PSIII-38 Effect of Deficiency or Surplus Dietary Energy Intake on the Onset of Puberty in Gilts Investigated by Liquid Chromatography-Mass Spectrometry Based Follicular Fluid Metabolomics Analysis.

150 Liquid Chromatography-Mass Spectrometry-Based Serum Metabolomics Analysis of the Effect of Dietary Energy Intake on the Onset of Puberty in Gilts.

150 Liquid Chromatography-Mass Spectrometry-Based Serum Metabolomics Analysis of the Effect of Dietary Energy Intake on the Onset of Puberty in Gilts.

Effects of Dietary Glucose on Serum Estrogen Levels and Onset of Puberty in Gilts.

Metabolic signals and the state of energy reserves have been shown to play a crucial role in the regulation of reproductive function. This study was carried out to investigate the effects of dietary glucose levels on puberty onset in gilts. Weight-matched, landrace gilts (n = 36) 162±3 days old, weighing about 71.05±4.53 kg, were randomly assigned to 3 dietary treatment groups of 12 gilts each. The trial lasted until the onset of puberty. Gilts in each group were supplied with diets containing different levels of glucose as follows: i) starch group (SG) was free of glucose, contained 64% corn derived starch; ii) low-dose group (LDG) contained 19.2% glucose and 44.8% corn derived starch; iii) high-dose group (HDG) contained 30% glucose and 30% corn derived starch. Results indicated: i) The growth performance of gilts were not affected by the addition of glucose, but the age of puberty onset was advanced significantly (p<0.05); ii) Compared with the SG, the concentration of insulin significantly increased before puberty in HDG (p<0.05); iii) There was no difference in serum progesterone (P) levels amongst the different feed groups, however, levels of estradiol (E2), luteinizing hormone, and follicle-stimulating hormone were significantly higher at puberty onset in HDG (p<0.05). Overall, our findings indicate that glucose supplementation significantly advances puberty onset, which can have practical purposes for commercial breeding.

Effects of birthweight on reproductive system development and onset of puberty in gilts.

The aim of the present study was to investigate the effects of birthweight on bodyweight development, development of the genital tract, onset of puberty and their associations with insulin-like growth factor (IGF) 1 and leptin concentrations. Pairs of littermate gilts from 51 litters were selected: one piglet with the highest birthweight (HW; 1.5±0.2kg) and the other with the lowest birthweight (LW; 1.0±0.2kg). Gilt pairs were killed at either fixed ages (80.8±1.2 days; AG; 16 pairs), fixed bodyweight (35.2±1.4kg; WG; 16 pairs) or after first oestrus (EG; 19 pairs). In the AG group, HW gilts were 5.6kg heavier at the time of death than LW gilts. In the WG group, LW gilts were 5.9 days older at the time of death (P<0.05). There were no significant differences in the number or size of total antral follicles or in the follicle population among birthweight classes. Age at puberty was similar between the HW and LW gilts, but bodyweight at time of death was greater for HW gilts (P<0.05). Birthweight did not affect the development of the genital tract, ovulation rate or hormone plasma concentrations. These results suggest that birthweight does not affect the development of the genital tract before puberty and puberty onset.

Melatonin implants do not alter estrogen feedback or advance puberty in gilts

Puberty in pigs is often delayed during late summer and autumn, with long daylength the most likely cause. We hypothesised (1) that gilts born around the shortest day would have a later release from the negative feedback actions of estradiol than gilts born around the spring equinox and (2) melatonin treatment would result in an earlier release from estradiol negative feedback and advance the onset of puberty in gilts born around the spring equinox. We first determined the optimal number of estradiol implants required to monitor the release from estradiol negative feedback in ovariectomised gilts. Secondly we determined whether melatonin implants altered negative feedback in 4 cohorts of ovariectomised gilts born between the winter solstice and spring equinox, and in the following year whether melatonin altered the time of the first ovulation in 5 cohorts of intact gilts born between the winter solstice and spring equinox. Plasma LH and FSH increased between 126 and 210d of age (P<0.001) in each cohort (season), but there was no effect of cohort, melatonin treatment or interactions (P>0.05). Age at first detection of elevated plasma progesterone in untreated, intact gilts decreased across the 4 cohorts (P<0.05). Melatonin treatment of intact gilts failed to advance the age of puberty irrespective of their season of birth (P>0.05). In conclusion, while we confirmed that estradiol sensitivity is decreased as gilts age, we failed to demonstrate any effects of season or melatonin on estradiol feedback or melatonin on puberty.

Prepuberal evolution of plasma leptin levels in gilts of thrifty genotype (Iberian pig) and lean commercial crosses (Large White × Landrace)

Current study aimed to determine possible differences in plasma leptin levels during the prepuberal period and their relationship with the onset of puberty in gilts of obese thrifty genotype (Iberian breed) and lean genotype (Large White×Landrace commercial crosses) reared under similar conditions. Plasma leptin concentration increased linearly during the 7weeks prior to the day of puberty attainment in both genotypes (P<0.005, r=0.707 for LW×L and P<0.0005, r=0.874 for Iberian gilts). However, leptin levels in the Iberian gilts was higher from the first sample of the experimental period, with females having 16weeks-old (2.7±0.3 vs 1.7±0.2ng/ml in LW×L; P<0.001), to the onset of puberty (8.5±0.7 vs 2.8±0.3ng/ml in LW×L; P<0.005). Thus, the current study reinforces previous data on changes in around puberty and evidences, for the first time, profound differences in prepuberal plasma leptin levels between gilts of obese (Iberian) and lean genotypes (LW×L).

Advanced Onset of Puberty in Gilts of Thrifty Genotype (Iberian Pig)

The Iberian pig is an autochthonous breed of the Mediterranean area, reared extensively in the central and southern areas of Spain and Portugal and that is known worldwide for the production of Iberian ham. The characteristics of the Iberian ham are related to its abundance of intramuscular fat, owing to the high capacity of the pig to accumulate fat under its skin and between the muscular fibres. This ability to store excess fat enables survival during periods of scarcity and it has been found in other antique animal breeds and even in humans, being named as the thrifty genotype. The reproductive management of the Iberian pig, in spite of a lack of accurate information unlike in other swine breeds, is based on the assumption of lower reproductive precocity and efficiency than the modern commercial breeds. The current study characterized and compared the onset of puberty in gilts of Iberian breed and meat commercial crosses (Large White × Landrace) reared in the same conditions by assessing weekly plasma progesterone profiles. At the end of the experimental period, when the gilts became 180 days old, the percentage of Iberian females that had reached puberty was 72.2%, with a mean age and weight of 160.5 ± 2.6 days and 92.9 ± 4.7 kg, respectively. The percentage of Large White × Landrace cross-breed females reaching puberty at 180 days was only 15% (p < 0.05). The mean age and weight were 165.0 ± 4.1 days and 107.8 ± 2.2 kg (p < 0.05), respectively. Thus, converse to the traditional assumption, onset of puberty was advanced in Iberian gilts. These results are important for the reproductive management of the Iberian pig but, at the same time, may set the basis for future studies on the metabolism-reproduction link and, specifically, on the possible relationship between earlier accumulation of fat and attainment of puberty.

Determination by echography of uterine changes around puberty in gilts and evaluation of a diagnosis of puberty.

Three experiments were carried out to evaluate the use of ultrasonography in assessing the onset of puberty in gilts. In experiment 1, gilts (n = 17) were scanned 3 times per week beginning at 133 and continuing until 187 days of age. The ultrasonic appearance of the uterus was described, quantified and compared with the reproductive status observed at slaughter. The quantification of the pictures showed a different correlation in time for infantile, impubertal, prepubertal and pubertal stages. For pubertal females, "uterine area" increased at around 180 days of age, well-defined sections of the uterine horns appeared 3 +/- 0.5 days before puberty. In infantile and impubertal gilts during the same period of age, uterine images remained dark and homogeneous; no significant change in the "uterine area" was observed. This difference in images allowed an evaluation of the diagnosis of puberty. In experiment 2, the gilts (n = 123) were scanned, the result was verified at slaughter the day after by examination of the genital tract. The uterine weight of the gilts that had reached a prepubertal or pubertal stage was significantly greater (P = 0.0001) than that in impubertal gilts. The sensitivity and the specificity of the diagnosis were 91.9% and 96.5% respectively. Experiment 3 was performed on a farm and echographic examinations were carried out one and five days after gilts (n = 117) arrived at the piggery. Oestrus detection or blood sampling for progesterone determinations were used as tools to determine the reproductive status. The sensitivity and the specificity of the diagnosis were 98.9% and 100% respectively. This diagnosis of puberty is thus accurate.

Failure of melatonin implants to alter onset of puberty in gilts.

Forty crossbred gilts (Hampshire x Yorkshire x Landrace x Duroc) weighing 55.4 +/- 0.5 kg at 4.2 +/- 0.1 months of age received either melatonin implants (N = 20) or served as controls (N = 20). Gilts were housed in a temperature controlled room (21 degrees C) with a light (L):dark (D) cycle of 10L:14D. Initially, four implants (12 mg melatonin each, Wildlife Pharmaceuticals, Ft. Collins, CO) were placed in one ear subcutaneously. At 2-week intervals, five, six, seven or eight implants were placed in ears of the gilts (one implant per 11.8 kg body weight). Throughout the trial, blood samples were collected twice weekly by jugular venipuncture. All gilts were exposed to mature boars for 15 min on alternate days. Age of puberty was determined from serum concentrations of progesterone and visual observations of estrus. Serum concentrations of melatonin were elevated (5-10 fold) throughout the trial in those gilts that received melatonin implants. Average daily gain (P = 0.51) and age at puberty (P = 0.57) were similar between gilts that received melatonin implants or no implants. Even though gilts continued to receive melatonin after they attained puberty, elevated serum concentrations of melatonin did not alter the length of subsequent estrous cycles (P = 0.90). These data indicate that elevation of serum concentrations of melatonin via implants did not alter the onset of puberty or length of subsequent estrous cycles.

Variation in age at puberty in pigs with divergent selection for components of efficient lean growth rate

A delay in the onset of puberty in gilts with intensive selection for efficient lean growth would have substantial implications for pig breeding programmes due to later first parity matings. Therefore the current study determined the responses in gilt traits at puberty to selection for components of efficient lean growth rate.

Effect of exogenous porcine prolactin (pPRL) on LH plasma concentration and on the release of GnRH in vitro from the stalk median eminence in prepubertal gilts

Abstract The aim of this study was to determine the effect of exogenous porcine prolactin (pPRL) induced hyperprolactinemia on the level of luteinizing hormone (LH) in blood, on the concentration of LH/human chorionic gonadotrophin (hCG) receptors in ovaries and on the release of gonadotrophin-releasing hormone (GnRH) in vitro from the stalk median eminence (SME) in prepubertal gilts. The experiment was performed on 22 prepubertal gilts (Large White), at 155 days of age (mean ± SD body weight 60.1 ± 3.4 kg). The animals were randomly assigned to two groups: one received 5 mg of PRL (BIOLACTIN, Biochefa, Sosnowiec, Poland, n = 11) and the other received saline (n = 11) i.m. once a day for 21 days. Blood samples were collected from the jugular vein three times a day during the first 2 weeks, four times a day in the third week of the study, additionally every 20 min for 4 h (08:00–12:00 h) and every 2 h for 8 h on days 1, 7, 15 and 21 of the experiment. Concentrations of PRL and LH in plasma and GnRH in medium were analyzed by radioimmunoassay (RIA) procedures. All gilts were slaughtered on the 21st day of treatment. Ovaries and hypothalamus were dissected immediately following slaughter. Mean concentrations of LH in the consecutive weeks of the study were similar in the PRL-treated gilts whereas in the control animals they rose significantly (from 0.29 ± 0.02 ng ml−1 the first week to 0.40 ± 0.02 ng ml−1 in the third week; P The results indicate that PRL treatment stimulated a release of LH from the pituitary gland in prepubertal gilts and slightly elevated the number of LH/hCG receptors in the ovaries. These findings suggest that PRL may influence processes connected with the onset of puberty in gilts.

Melatonin and farm animals: Endogenous rhythms and exogenous applications

Studies on farm animals have contributed significantly to our increased understanding of basic melatonin-related physiological mechanisms, as well as to the regulation of reproduction and pelage in individual domestic species. This review concentrates on recent work on the role of melatonin in the regulation of porcine reproduction, cervine endocrine, and behavioral cycles and wool and cashmere production which has added to this knowledge base. Early studies of the domestic pig indicated that melatonin secretion in this species differed markedly from that in other domestic and laboratory animals. There is now clear evidence that this is not the case and that the domestic pig uses a circadian rhythm of melatonin release for the transduction of photoperiodic information. Apparent inconsistencies among reports may be due, in part, to differences in the conditions under which the experiments were performed and to the assay systems employed to measure circulating melatonin, the concentrations of which are much lower than in other domestic species. Appropriately administered exogenous melatonin advances the onset of puberty in gilts, and may prove to be effective in overcoming seasonal infertility in female pigs. Appropriately timed melatonin and/or photoperiod treatments, administered to ruminants in utero, influence the reproductive physiology of the offspring, indicating that even in species which don't develop an endogenous melatonin rhythm till some weeks postnatally, awareness of photoenvironment, presumably via maternal melatonin, predates birth. Pre- or early postnatal melatonin-related treatments also influence the development/cycle frequency of pelage. Areas requiring further investigation include the hormonal/growth factors involved, reasons for the transient nature of the effects in sheep and goats, and the reason for similar effects on pelage of augmenting or inactivating melatonin. Aspects of endogenous melatonin rhythms in farm species which require further study include: the significance of the abolition of the nocturnal melatonin peak in the sheep by prolonged short day exposure; the increased pineal bloodflow in sheep bred to produce high wool yields; the presence of high daytime melatonin levels immediately prior to the rut in the fallow buck; and the low amplitude of the rhythm in the domestic pig.

A Survey of the concentration of ammonia recorded in pig housing in England

In animal housing ammonia is an aerial pollutant resulting from the microbial conversion of nitrogenous materials excreted in faeces and urine. Ammonia is an irritant to cells lining the respiratory tract and an increased incidence of pneumonia in pigs and humans and atrophic rhinitis in pigs have been correlated with elevated ammonia levels. In a survey of 12 farms and 49 pig buildings concentration of ammonia was identified specifically as a causative agent in the aetiology of atrophic rhinitis (Robertson, Wilson and Smith 1990). Furthermore, high levels of ammonia can delay the onset of puberty in gilts perhaps as a result of the masking of boar odour (Malayer, Brandt, Green, Kelly, Sutton and Diekman 1988).Regulations have been introduced (Control of Substances Hazardous to Health (COSHH)) limiting the exposure of humans to atmospheric ammonia to a mean level of 25 ppm over an 8h work shift and 35 ppm for 10 minute exposure. As part of a study on the effectiveness of a feed additive formulated to control atmospheric ammonia, levels of ammonia in pig housing were monitored.

Reduction in age of puberty in gilts consuming melatonin during decreasing or increasing daylength.

Two experiments were conducted to determine whether oral administration of melatonin alters the onset of puberty in gilts during naturally increasing or decreasing daylength. In Exp. 1, 20 crossbred prepubertal gilts weighing 77.5 +/- .5 kg at 171.8 +/- 1.0 d of age were assigned randomly to receive either a daily oral dose of 3 mg of melatonin (MEL) or ethanol vehicle (ETH) at 1530 from August 31 to December 1, 1987 (decreasing daylength). Gilts were exposed to mature boars for 20 min thrice weekly and blood samples were collected twice weekly. Serum concentrations of progesterone were used to establish age at puberty and length of estrous cycle. In Exp. 2, 20 crossbred prepubertal gilts weighing 67.7 +/- .7 kg at 143.8 +/- 1.1 d of age received either MEL or ETH treatment from February 1 to May 15, 1988 (increasing daylength). Age of puberty was less in gilts that received MEL than in gilts that received ETH in both Exp. 1 (198 +/- 3 vs 228 +/- 7 d; P less than .01) and Exp. 2 (183.8 +/- 2.7 d vs 194.3 +/- 3.3 d; P less than .05). Gilts that received MEL reached puberty at a lighter weight than gilts that received ETH in Exp. 1 (95.6 +/- 2.1 vs 112.4 +/- 3.9 kg; P less than .01) and Exp. 2 (88.1 +/- 1.5 vs 96.0 +/- 1.8 kg; P less than .01). Serum concentrations of LH and FSH, length of estrous cycles, and percentage of muscle of carcasses were similar between MEL and ETH gilts.(ABSTRACT TRUNCATED AT 250 WORDS)

Influence of manure gases on the onset of puberty of replacement gilts

AbstractTo determine whether gases generated during the breakdown of accumulated manure in an environmentally regulated building affects the onset of puberty in gilts, 42 crossbred gilts, born from 1 o t 15 September 1985, were reared indoors from 10 to 30 weeks of age on concrete slats over a pit that was drained and refilled with clean water biweekly (clean group). Forty-two gilts were reared over a pit where manure was allowed to accumulate (control group). These two groups had similar feeding, water, floor space, lighting and room temperature. A third group of 42 crossbred gilts was reared from 10 to 30 weeks of age in an open-front building with a concrete apron (outdoor group). Concentration of aerial ammonia in the control environment was three- to five-fold higher than in either the clean or the outdoor environment (P &lt; 0·001). Average daily gain and food conversion efficiency were similar for the two indoor treatment groups, but the outdoor group gained less weight than either indoor group (P &lt; 0·05). At 26 weeks of age all gilts were exposed to mature boars daily for 2 weeks, then every 2nd day for an additional 2 weeks. A greater proportion of gilts in the clean environment attained puberty 7 days (P &lt; 0·05) and 10 days (P &lt; 0·07) after first exposure to boars. These data suggest that odorous gases, such as ammonia, in the air of environmentally regulated buildings may diminish the stimulatory influence of boars on the onset of puberty in gilts.

A note on the influence of a Göttingen miniature boar on puberty attainment in Large White gilts

The initial work of Brooks and Cole (1970), which showed that a mature boar advances the onset of puberty in gilts, has become known as the ‘boar effect’, and this phenomenon has now been confirmed in numerous studies as reviewed by Hughes (1982), and Kirkwood and Hughes (1982).

Responses of seasonally anestrous gilts and weaned primiparous sows to treatment with pregnant mare's serum gonadotropin and Altrenogest

Seasonal anestrus during late summer and autumn delays the onset of puberty in gilts and the prompt return to estrus in weaned sows. In this study, 132 gilts that had not been detected in estrus within 3 wk after being moved to the breeding barn were given a single intramuscular (i.m.) injection of 0, 363, 725, or 1088 IU of pregnant mare's serum gonadotropin (PMSG), or they were fed Altrenogest, a synthetic progestogen, (20 mg per day) for 14 d. Thirty eight percent of control gilts were detected in estrus within 20 d after treatment. Treatment with 725 or 1088 IU PMSG or Altrenogest significantly increased the proportion of gilts detected in estrus within 20 d to 77% and significantly reduced the average interval to estrus. Treatment with 363 IU PMSG did not increase the percentage of gilts in estrus within 20 d. Primiparous sows (n = 155) were assigned to a 2 × 3 factorial experiment at weaning. The sows received 0 or 20 mg Altrenogest for 3 d beginning the day after weaning and 0, 900, or 1200 IU PMSG which was given the day after weaning or the day after the last dose of Altrenogest was administered. Fifty eight percent of control sows were detected in estrus within 10 d. Administration of 1200 IU PMSG alone increased the percentage of sows detected in estrus within 10 d to 95%. Treatment with 1200 IU PMSG plus Altrenogest or 900 IU PMSG with or without Altrenogest tended to increase the percentage of sows in estrus within 10 d (70 to 76%). Altrenogest alone was not beneficial. Fertility and subsequent litter sizes among gilts and sows that were given various treatments were similar. We concluded that a single intramuscular injection of an appropriate dose of PMSG overcomes seasonal anestrus in gilts and weaned primiparous sows.

Effects of composition and density of the group on the performance, behaviour and age at puberty in swine

Two hundred and eighteen cross-bred pigs (Duroc × Hampshire × Landrace × Yorkshire) were assigned to a 3 × 2 factorial experiment; the factors being composition of the group and density. The composition of the group factor consisted of pigs not reaggregated (NR), re-aggregated below the median body weight (RBMW), and re-aggregated above the median body weight (RAMW) at the time of transfer to the finishing pens. The density factor consisted of groups of 6 or 12 pigs/pen. Two trials with 2 replications of each trial were conducted. Twenty-six gilts from Trial I were used to determine their age and ovulation rate at the pubertal oestrus. During the nursery phase, composition of the group had no effect ( P>0.05) on average daily feed intake (ADFI) or feed efficiency (F/G). Pigs in the NR and RAMW groups had a similar ( P>0.05) average daily gain (ADG), whereas, those pigs in the RBMW group gained ( P<0.05) at a slower rate. Pigs housed in groups of 6 had a higher ( P<0.05) ADFI, had similar ( P>0.05) ADG and poorer ( P<0.05) F/G than those housed in groups of 12. During the finishing phase, composition of the group had no effect ( P>0.05) on ADFI, ADG or F/G. Pigs housed in groups of 6 had a greater ( P<0.05) ADFI and ADG, but similar ( P<0.05) F/G compared to those housed in groups of 12. Correlation coefficients between the various behaviours and the performance variables suggested that the more active pens of pigs gained less per day and were less efficient. Gilts housed in the NR groups of 6 were younger ( P<0.05) at puberty than gilts in the other groups of 6 or 12. Also, gilts housed in the RBMW groups of 6 were younger ( P<0.05) at puberty than those housed in the RAMW groups of 6, whereas those housed in the RBMW groups of 12 were older ( P<0.05) at puberty than those housed in the RAMW groups of 12. Ovulation rate was not affected ( P>0.05) by composition of the group, density or their interaction. In summary, composition and density of the group affected the performance of pigs and was correlated to some of the behavioural measurements. The most noticeable effect was that these same factors caused a delay in the onset of puberty in gilts.