Event Abstract Back to Event Functional imaging at cellular level in hemicochlea preparation from hearing mice. M. Aller1*, R. Fekete1, T. Horváth1, G. Polony1, Á. Fekete1, G. Halmos1, A. Heinrich1, B. Sperlágh1, B. Lendvai1, E. S. Vizi1 and T. Zelles1 1 Hungarian Academy of Sciences, Institute of Experimental Medicine, Hungary Hearing loss (HL) is the most frequent sensory deficit. In contrast to conductive forms, there is no effective treatment for sensorineural hearing losses (SNHLs; e.g. noise-induced HL or presbycusis). One of the main reason for the absence of tools to prevent and cure SNHLs is the insufficient knowledge of basic molecular mechanisms of normal and impaired adult hearing. Cells of the organ of Corti and auditory neurons are pimary targets in SNHLs. Majority of SNHLs are developed after the onset of hearing but investigations at cellular level in mice are usually performed before that. Hemicochlea preparation of hearing mice (>P14) has a structure and metabolism that are closer to in vivo conditions than any other preparations, including isolated cells, ex vivo explants or organotypic cultures from immature ears. We have developed a method of fluorescence imaging in acute hemicochlea preparation using various fluorescence indicators. After loading of the preparation with Fura-2/AM, we could measure repeatable Ca2+ transients in individual cells of the organ of Corti evoked by perfusion of ATP. Using dihydroethidium, a superoxide indicator we detected superoxide production in response to neurotoxic levels of Glu in auditory neurons. Disturbed regulation of Ca2+ and ROS are involved in SNHLs. However, precise description of the mechanisms is missing. Our new method offers the appropriate spatial and temporal resolution to explore their function in the damage of the inner ear. Keywords: methods, Neuroscience Conference: 13th Conference of the Hungarian Neuroscience Society (MITT), Budapest, Hungary, 20 Jan - 22 Jan, 2011. Presentation Type: Abstract Topic: Methods Citation: Aller M, Fekete R, Horváth T, Polony G, Fekete Á, Halmos G, Heinrich A, Sperlágh B, Lendvai B, Vizi ES and Zelles T (2011). Functional imaging at cellular level in hemicochlea preparation from hearing mice.. Front. Neurosci. Conference Abstract: 13th Conference of the Hungarian Neuroscience Society (MITT). doi: 10.3389/conf.fnins.2011.84.00080 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 03 Mar 2011; Published Online: 23 Mar 2011. * Correspondence: Dr. M. Aller, Hungarian Academy of Sciences, Institute of Experimental Medicine, Budapest, Hungary, allerm@koki.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers M. Aller R. Fekete T. Horváth G. Polony Á. Fekete G. Halmos A. Heinrich B. Sperlágh B. Lendvai E. S Vizi T. Zelles Google M. Aller R. Fekete T. Horváth G. Polony Á. Fekete G. Halmos A. Heinrich B. Sperlágh B. Lendvai E. S Vizi T. Zelles Google Scholar M. Aller R. Fekete T. Horváth G. Polony Á. Fekete G. Halmos A. Heinrich B. Sperlágh B. Lendvai E. S Vizi T. Zelles PubMed M. Aller R. Fekete T. Horváth G. Polony Á. Fekete G. Halmos A. Heinrich B. Sperlágh B. Lendvai E. S Vizi T. Zelles Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.