The integration of Artificial Intelligence (AI) into education is a recent development, with chatbots emerging as a noteworthy addition to this transformative landscape. As online learning platforms rapidly advance, students need to adapt swiftly to excel in this dynamic environment. Consequently, understanding the acceptance of chatbots, particularly those employing Large Language Models (LLM) such as Chat Generative Pretrained Transformer (ChatGPT), Google Bard, and other interactive AI technologies, is of paramount importance. Investigating how students accept and view chatbots is essential to directing their incorporation into Industry 4.0 and enabling a smooth transition to Industry 5.0's customized and human-centered methodology. However, existing research on chatbots in education has overlooked key behavior-related aspects, such as Optimism, Innovativeness, Discomfort, Insecurity, Transparency, Ethics, Interaction, Engagement, and Accuracy, creating a significant literature gap. To address this gap, this study employs Partial Least Squares Structural Equation Modeling (PLS-SEM) to investigate the determinant of chatbots adoption in education among students, considering the Technology Readiness Index and Technology Acceptance Model. Utilizing a five-point Likert scale for data collection, we gathered a total of 185 responses, which were analyzed using R-Studio software. We established 12 hypotheses to achieve its objectives. The results showed that Optimism and Innovativeness are positively associated with Perceived Ease of Use and Perceived Usefulness. Conversely, Discomfort and Insecurity negatively impact Perceived Ease of Use, with only Insecurity negatively affecting Perceived Usefulness. Furthermore, Perceived Ease of Use, Perceived Usefulness, Interaction and Engagement, Accuracy, and Responsiveness all significantly contribute to the Intention to Use, whereas Transparency and Ethics have a negative impact on Intention to Use. Finally, Intention to Use mediates the relationships between Interaction, Engagement, Accuracy, Responsiveness, Transparency, Ethics, and Perception of Decision Making. These findings provide insights for future technology designers, elucidating critical user behavior factors influencing chatbots adoption and utilization in educational contexts.