Narcolepsy remains underrecognized and underdiagnosed despite affecting approximately 1 in every 3000 Americans. A study was undertaken to evaluate the effectiveness of an online educational intervention with the goal of improving neurologists’ knowledge of diagnostic criteria for narcolepsy issued by the American Psychiatric Association (DSM 5) and American Academy of Sleep Medicine (ICSD 3). The online continuing medical education (CME) activity consisted of a 30-minute video discussion between two faculty on how to diagnose narcolepsy. Educational effect was assessed by comparing a matched sample of neurologists’ responses to 4 identical questions presented before and directly after exposure to the intervention. A chi-square test was used to identify significant differences between pre- and post-assessment responses. P values <0.05 were considered statistically significant. Cramer’s V was used to calculate the effect size of the online education. Data was collected between January 26, 2016, and March 22, 2016. Participation in the CME intervention improved knowledge of neurologists as indicated by the medium educational effect size (n = 123; V=0.184; P <0.05). As a result of their participation in this educational intervention, significant overall improvements (P<0.05) were observed pre- vs post-participation in several specific areas, including knowledge of ICSD 3 criteria to diagnose type 1 narcolepsy (220% relative improvement), differentiation between the DSM 5 and ICSD 3 regarding the diagnostic criteria for narcolepsy (23% relative improvement), and knowledge of the clinical utility of the Swiss Narcolepsy Scale (118% relative improvement). No significant improvement was found regarding the identification of core symptoms of narcolepsy that typically accompany excessive daytime sleepiness. The results indicated that the CME-certified 30-minute video discussion between two narcolepsy experts was effective at improving learner knowledge regarding guideline-based diagnostic criteria for narcolpesy. A significant improvement in knowledge was also demonstrated regarding the differences between the DSM 5 and ICSD 3 diagnostic criteria. Future education should continue to address the spectrum of symptoms indicative of narcolepsy. An unrestricted educational grant from Jazz Pharmaceuticals.