Onion Plants Research Articles

Coding complete sequence of three segments of Allium deltapartitivirus from Iraq.

Here, we report the coding complete sequence of three segments of the Iraqi isolate of Allium deltapartitivirus named Tikrit in onion plants. According to the phylogeny, this isolate is closely related to an Allium deltapartitivirus from Brazil and to Arhar cryptic virus 1 from Hyderabad.

Influence of plant species, mycorrhizal inoculant, and soil phosphorus level on arbuscular mycorrhizal communities in onion and carrot roots.

Arbuscular mycorrhizal fungi (AMF) are ancient and ecologically important symbionts that colonize plant roots. These symbionts assist in the uptake of water and nutrients, particularly phosphorus, from the soil. This important role has led to the development of AMF inoculants for use as biofertilizers in agriculture. Commercial mycorrhizal inoculants are increasingly popular to produce onion and carrot, but their specific effects on native mycorrhizal communities under field conditions are not known. Furthermore, adequate availability of nutrients in soils, specifically phosphorus, can reduce the diversity and abundance of AMF communities in the roots. The type of crop grown can also influence the composition of AMF communities colonizing the plant roots. This study aimed to investigate how AMF inoculants, soil phosphorus levels, and plant species influence the diversity of AMF communities that colonize the roots of onion and carrot plants. Field trials were conducted on high organic matter (muck) soil in the Holland Marsh, Ontario, Canada. The treatments included AMF-coated seeds (three to five propagules of Rhizophagus irregularis per seed) and non-treated onion and carrot seeds grown in soil with low (~46 ppm) and high (~78 ppm) phosphorus levels. The mycorrhizal communities colonizing the onion and carrot roots were identified by Illumina sequencing. Five genera, Diversispora, Claroideoglomus, Funneliformis, Rhizophagus, and Glomus, were identified in roots of both plant species. AMF communities colonizing carrot roots were more diverse and richer than those colonizing onion roots. Diversispora and Funneliformis had a 1.3-fold and 2.9-fold greater abundance, respectively, in onion roots compared to carrots. Claroideoglomus was 1.4-fold more abundant in carrot roots than in onions. Inoculation with R. irregularis increased the abundance and richness of Rhizophagus in AMF communities of onion roots but not in carrot roots. The soil phosphorus level had no effect on the richness and diversity of AMF in the roots of either crop. In summary, AMF inoculant and soil phosphorus levels influenced the composition of AMF communities colonizing the roots of onion and carrot plants, but the effects varied between plant species.

Identifying Onion Fields at Risk of Iris Yellow Spot Virus in New York.

Iris yellow spot virus (IYSV) poses a significant threat to dry bulb onion, Allium cepa L., production and can lead to substantial yield reductions. IYSV is transmitted by onion thrips, Thrips tabaci (Lindeman), but not via seed. Transplanted onion fields have been major early season sources of IYSV epidemics. As onion thrips tend to disperse short distances, seeded onion fields bordering transplanted onion fields may be at greater risk of IYSV infection than seeded fields isolated from transplanted ones. Additionally, seeded onion fields planted early may be at greater risk of IYSV infection than those seeded later. In a 2-year study in New York, we compared IYSV incidence and onion thrips populations in seeded onion fields relative to their proximity to transplanted onion fields. In a second study, we compared IYSV incidence in onion fields with either small or large plants during midseason. Results showed similar IYSV incidence and onion thrips populations in seeded onion fields regardless of their proximity to transplanted onion fields, while IYSV incidence was over four times greater in large onion plants than in small ones during midseason. These findings suggest a greater risk of onion thrips-mediated IYSV infection in onion fields with large plants compared with small ones during midseason and that proximity of seeded fields to transplanted ones is a poor indicator of IYSV risk. Our findings on IYSV spread dynamics provided valuable insights for developing integrated pest and disease management strategies for New York onion growers.

Increasing Red Onion Plant (Allium ascalonicum L) Growth and Production by Providing Cascing and NPK 16.16.16. Fertilizer

Riau Province relies on other regions, such as North Sumatra, West Sumatra, and Java, for its needs. However, when natural disasters occur, or transportation disruptions happen from the sources of onion production, the price of shallots in the market increases. To reduce Riau companies' dependency on this raw material, it is necessary to develop shallot plants using optimal cultivation techniques to maximize growth and production. This research was conducted at the Experimental Garden of the Faculty of Agriculture, Pasir Pengaraian University, Rokan Hulu Regency, for three months from May 2022 to July 2022. The research utilized a completely randomized factorial design with two factors. The first factor involved the application of Vermicompost Fertilizer, with four treatment levels: 0. 200. 400. and 600 g/plot. The second factor involved the application of NPK 16.16.16 fertilizer with four treatment levels: 0. 10. 20. and 30 g/plot. The parameters observed included plant height, harvest age, number of tubers per cluster, tuber weight per bulb, wet tuber weight per cluster, dry tuber weight, and percentage of tuber weight loss. The research results indicated that the interaction between vermicompost fertilizer and NPK 16.16.16 fertilizer had a significant impact on plant height, harvest age, number of tubers per cluster, number of tubers per tuber, wet tuber weight per cluster, and dry tuber weight per cluster. The best treatment was a combination of Vermicompost 600 g/plot and NPK 16.16.16 30 g/plot. The main effect of the Vermicompost Fertilizer application was significant for all observation parameters, and the main effect of NPK 16.16.16 also had a significant impact on all observation parameters.

First Report of Stemphylium vesicarium Causing Onion Stemphylium Leaf Blight in Mauritius.

In August 2021, severe leaf blight symptoms were observed on onion (Allium cepa L. cvs Francia and Askari F1 hybrid) in commercial fields located in Mauritius, namely La Forêt (20°19'56.1"S57°30'04.9"E), St Aubin (20°29'47.0"S57°32'29.4"E) and Chapiron (20°20'46.8"S 57°29'12.8"E). Infected leaves displayed small circular to oblong yellow-pale-brown and spindle shaped lesions which later coalesced and formed necrotic areas with black sporulation. Three fields were selected from each region, and along a W-pattern across the fields a disease incidence ranging 53-93% and a severity of 9-28% were recorded. Ten symptomatic leaves were collected in each region and small pieces of infected tissue were surface disinfected using 1% NaOCl for 2 min, rinsed with sterile distilled water, air-dried, transferred to potato dextrose agar (PDA) and incubated for 7 days at 20°C under a 12-h light/dark cycle. Fungal cultures with uniform appearance forming multi-septated conidia typical of the genus Stemphylium (Simmons 1969) were consistently isolated. Monosporic colony of isolates SVCWLF24/3, SVSSA23/1 and SVCWLMC26/1 developed similar olivaceous green to light and dark grey mycelium with an average daily growth rate of 6.5 mm at 25°C in the dark. Conidiophores were straight, light brown with a distinct swollen apex on which olive brown to dark brown, oblong to ovoid, septate conidia formed with dimensions 16.2-44.7 × 8.0-22.9 μm (av. 29.5 x 14.7 μm; n = 50) typical of Stemphylium vesicarium (Wallr) E.G. Simmons 1969 (Woudenberg et al. 2017). Genomic DNA of the three isolates was extracted from fungal mycelium (Ranghoo and Hyde 2000).. The ITS, cmdA and gapdh genes of the isolates were amplified with primers ITS4/ITS5 (White et al. 1990), CALDF1/CALDR1 (Lawrence et al. 2013) and Gpd1/Gpd2 (Beerbee et al. 1999) and sequenced. Sequences were submitted to GenBank under accession numbers OR131271, ON620213, OR188702 (ITS), OR350623, OR350622, OR166368 (cmdA) and OR684516, OR684517, OR684518 (Gapdh). The BLAST search of the sequences showed 100% similarity with S. vesicarium strain CBS 155.24 under accession numbers KU850555 (ITS), KU850702 (Gapdh) and KU850845 (cmdA) (Woudenberg et al. 2017). Phylogenetic trees inferred from the ITS, cmdA and Gapdh concatenated datasets with the maximum-likelihood algorithm allowed clustering of the isolates within S. vesicarium clade, confirming the morphological identification. Pathogenicity tests were performed using all three isolates, cultured on PDA at 25°C in a 12-h dark/light cycle. Ten 60-day-old onion plants (cv. Francia) were spray inoculated each with 10 ml of conidial suspension (1 × 104/ml) of each isolate while 10 healthy plants sprayed with sterile distilled water served as control. They were incubated in a greenhouse at 25°C with a 12-h photoperiod and > 80% humidity. Necrotic circular lesions appeared on leaves after 7-10 days while control plants remained symptomless. Re-isolations made from symptomatic leaf tissues on PDA consistently yielded cultures with similar morphology as the original isolates, thus fulfilling Koch's postulates. This is the first report of S. vesicarium as the causal agent of leaf blight of onion in Mauritius. It is a re-emerging fungal disease (Hay et al. 2021) with a wide host range threatening local onion production. This finding will contribute to early detection of leaf blight, implementation of surveillance and integrated disease management in affected regions.

Enhancing bulb yield through nitrogen fertilization and the use of hybrid onion (Alluim cepa L.) varieties in northwest Ethiopia.

Onions are among the most important cash crops in developing countries, including Ethiopia. However, its production and productivity are very low, which is associated with inappropriate fertilization and the use of low-yielding varieties. Therefore, the present study was conducted to evaluate the effects of the nitrogen fertilizer rate on the growth, yield, and quality of hybrid onion varieties in northwest Ethiopia. The experiment was conducted at three locations (Koga, Woreta, and Woramit) during the 2021/2022 cropping season under irrigated conditions. The treatments consisted of three hybrid onion varieties (Russet, Jambar, Red Coach) and one open-pollinated onion variety (Bombay Red) and four nitrogen rates (0, 41, 82, and 123 kg ha-1), which were laid out in a randomized complete block design with a factorial arrangement of 4*4 in three replications. The results of the present study revealed that onion growth, yield and quality were influenced by the nitrogen fertilizer rate and onion variety across all locations. Compared with the open pollinated Bombay Red variety, the hybrid varieties (Russet and Jambar) performed well in terms of bulb diameter, bulb weight, total yield, marketable bulb yield, and pungency. Nitrogen fertilizer applied at a rate of 82 kg ha-1 resulted in the highest growth and yield parameters of onion. The Russet and Jambar varieties recorded the highest marketable bulb yields of 26.50 t ha-1 and 24.57 t ha-1, respectively. Onion varieties treated with the highest nitrogen fertilizer dosage of 123 kg ha-1, particularly the Bombay Red variety, exhibited the longest duration to reach maturity. Onion plants supplied with 82 kg ha-1 nitrogen presented the highest marketable bulb yields, with a value of 26.77 t ha-1. Too much nitrogen above 82 kg ha-1 leads to decreased yield; hence, excess nitrogen is lost to the environment. Furthermore, the Jambar and Russet hybrid varieties and the application of 82 kg ha-1 nitrogen fertilizers provided the highest net benefit. The hybrid varieties Jambar and Russet and the application of 82 kg ha-1 nitrogen fertilizer can be recommended for onion production in the study area and areas with similar agroecosystems. Since this study is the first of its kind, considering other hybrid onion varieties and optimizing agronomic practices such as spacing and phosphorus fertilizer are also recommended in future research.

Effect of planting time and seedling feeding area on the yield and biochemical composition of leek (Allium porrum L.)

In the article, the results of the research carried out in order to study the effect of planting time and feeding area on the biochemical composition of leek (Allium porrum L.) are presented with an in-depth scientific analysis. Planting was done by hand in 4x1 cm rows in 7x40x60 cm boxes in the greenhouse on February 20, March 10, and April 1. Seedlings appeared after 10-12 days, on May 1 they were transplanted to open ground. This ensured that the seedlings were 70, 50, and 30 days old. Planting in open ground was carried out in a scheme of 50x10 cm. Harvesting took place on September 1-5. At the end of the research, the data analysis shows that by evaluating the nature of growth of false stem diameter in leek onion plants at different planting periods during the growing season, it can be noted that the feeding area for seedlings is larger and the seedlings when planted early, standard products had earlier maturity and higher product quality.

Characteristics and Agronomic Efficiency of Fertilizer Urea Granules- Acidic Coated Bamboo Biochar Acrylate on Plant Onion Red (Allium ascaloniucm L.)

The use of urea-biochar granular fertilizer is intended to overcome the problem of inefficiency in the excessive use of urea fertilizer. The efficiency of urea is relatively low because 40-70% of N is lost from the soil. The purpose of this study was to determine the agronomic efficiency of acrylic acid-coated bamboo urea-biochar fertilizer on shallot plants. The research was conducted in the greenhouse of UPT Plant Pest and Disease Control Center in Celuk Sukawati Gianyar Village, and in the Laboratory of Soil and Environmental Sciences, Faculty of Agriculture, Udayana University, arranged in a Completely Randomized Design with a single factor consisting of 9 treatments and repeated 3 times. Parameters observed were plant growth yields (height, weight of tubers and fresh litter, weight of tubers and oven-dried litter, number of tillers), fertilizer agronomic efficiency. The results showed that the S30 treatment significantly increased the shallot plant height by 43.67 cm, the weight of the oven-dried bulbs by19.68 g and the RAE value by 362%, so that fertilization with the S30 treatment was the most efficient compared to other treatments and could be used by farmers in increasing the growth and yield of shallot plants.

Identification of Alternaria, Aspergillus, Fusarium and Penicillium spp. on Onion Plant (Allium cepa L.) Growing in Hatay, Amasya and Tokat Provinces Using MALDI-TOF Mass Spectrometry

Plant fungal disease pathogens cause significant yield and quality losses in onion growing areas. In addition to yield losses, they cause negative effects that reduce the quality and export potential of the product, resulting in significant economic losses during harvest, post-harvest, processing and marketing stages. In recent years, Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) has emerged as a rapid, cost-effective, reproducible, and powerful technique for identifying microorganisms, and its impact on microbiological diagnosis has transformed workflow in equipped laboratories. In this study, proteomic analyzes were performed on Alternaria, Aspergillus, Fusarium and Penicillium species isolated from onion growing areas in Hatay, Amasya, and Tokat provinces. After extraction of mycelium from single spore cultures of the isolates with ethanol-formic acid, the spectra of the individual fungal isolates were determined using the Flex control software program. These spectra were compared with Maldi Biotyper Real-Time Classification (RTC) and identification was performed. Of 519 different fungal isolates, 435 representative fungal isolates (83.8%) were identified by MALDI TOF MS. Eighty-four fungal isolates could not be identified because they were not in a satisfactory range of purity and identification. Of the 435 isolates, 269 (61.8%) were identified as Fusarium spp., 80 isolates (18.4%) were identified as Alternaria spp., 60 isolates (13.8%) as Aspergillus spp., and 26 isolates (6.0%) as Penicillium spp. Among the fungal isolates, 72.5% of the Fusarium isolates, 78.8% of the Alternaria isolates, 90.0% of the Aspergillus isolates and 84.6% of the Penicillium isolates were identified as “highly probable” species with score values between 2.000-3.000 (green color). These species are Alternaria alternata, Alternaria infectoria, Aspergillus flavus, Aspergillus niger, Fusarium culmorum, Fusarium moniliforme, Fusarium oxysporum, Fusarium proliferatum, Fusarium solani, Fusarium verticillioides, Penicillium commune and Penicillium glabrum. The results clearly demonstrate that MALDI TOF MS biotyping may be used as a highly reliable and economical diagnostic method for routine diagnosis of diseases caused by Alternaria, Aspergillus, Fusarium and Penicillium species.

CHARACTERIZATION OF GREEN ONION WITH NPK FERTILIZATION AND FOLIAR APPLICATION OF HORNWORT EXTRACT

A field trial on green onion crops in the autumn of 2020 transpired at a private farm in the District Al- Saniyah, Al-Diwaniyah Governorate, Iraq. The presented study aimed at characterizing green onion (Allium cepa L.) plants with NPK fertilization and foliar application of hornwort extract for growth and yield traits. In this experiment, the first factor comprised three NPK levels (0, 50%, and 100%) of NPK (150:100:150 kg ha-1) recommended fertilizer added to the soil, while the second factor included the foliar application of hornwort extract with two different concentrations (0, 20 ml L-1). The experiment in a randomized complete block design (RCBD) with factorial arrangement had three replications. Consequently, the recommended dose of NPK (100%) and spraying hornwort extract at 20 ml L-1 revealed a significant increase in the studied traits, i.e., plant height, length of the longest tubular leaf, number of leaves, fresh weight of tubular leaves, number of bulbs per plant, weight of the bulb, the diameter of the bulb, leaf chlorophyll content, bulb carbohydrate content, leaf carotenoid content, and plant yield. The interactions of both factors also showed a favorable effect on the mentioned traits in green onion.

Optimizing the Planting Date for Onion Production

This investigation was carried out during 2018 and 2019 at the research farm of the Agriculture Faculty of Kabul University, Afghanistan to study the correlation between weather conditions (maximum, minimum and mean temperature, relative humidity, cumulative day light and cumulative rainfall) and onion plant growth, yield and bulbs quality, as well as to find the optimal planting date for onion variety Safid e Paisaye. The data was analyzed with STAR software. The results of this study reveal that, increasing temperature and decreasing relative humidity and rainfall during growing season, caused faster maturity of bulbs, produced small sized bulbs, and finally reduced onion bulbs yield. It was observed that, due to relatively lower temperature and higher relative humidity during early growth stages the onion grown early in spring (seed sown in early March and transplants planted early in May) took longer period for maturation, produced larger bulbs and higher yield. This was also noted that due to longer maturation period the onion plants grown in early spring received a higher cumulative heat and sun light which contributed to improve bulbs quality and yield of onion. Based on this study results it is concluded that, early planting date of onion variety Safid e Paisaye not only increased significantly yield but also improved bulbs quality.

Increased Growth and Production of Shallots (Allium ascalonicum L.) with Mulching Type treatment and Tuber Weight

This study aims to determine the effect of mulch type and bulb weight on the growth and yield of onion production (Allium ascalonicum L.). In detail, this study aims to (1). Know the type of mulch that is most suitable for the growth and production of onion plants. (2). Knowing the weight of bulbs is best for the growth and production of onion plants. (3). Know the interaction between the type of mulch and the weight of bulbs used for the growth and production of onion plants. This study used Group Randomized Design (RAK) with 2 treatments, namely Factor I: three types of Mulch use (M): M0 = No Mulch; M1= Silver black plastic mulch (0.35 cm thick); M2= Mulch rice straw (1.5 cm thick). Factor II: three tuber weight intervals, namely: B0= 0 - 1.4 g (Small Tuber); B1= 1.5 – 1.8 g (Medium Tuber); B2 = > 1.8 g (Large Tubers). The results showed that the treatment of several types of mulch had a significant effect on plant height, weight of fresh tubers per clump, number of fresh tubers per clump, weight of fresh tubers per plot and number of fresh tubers per plot. M1 silver black plastic mulch treatment resulted in the highest plant height. weight of fresh tubers per clump (148.22 g), number of fresh tubers per clump, weight of fresh tubers per plot. Tuber weight treatment has a noticeable effect on plant height, number of leaves, weight of fresh tubers per clump, number of fresh tubers per clump, weight of fresh tubers per plot and number of fresh tubers per plot. B2 treatment yields the highest crop, number of leaves), weight of fresh tubers per clump, number of fresh tubers per clump, weight of fresh tubers per plot. The interaction of the use of several types of mulch and various tuber weights has a noticeable effect on plant height, number of leaves, weight of fresh tubers per clump, number of fresh tubers per clump, weight of fresh tubers per plot and number of fresh tubers per plot.

IMPLEMENTATION OF LSTM (LONG SHORT TERM MEMORY) ALGORITHM TO PREDICT WEATHER IN CENTRAL JAVA

Agro-indutrial agricultural production such as red onions in Indonesia has a very important share in driving Indonesia's economic growth, especially in Central Java province which contributed 28.15% of the total national red onion production in 2021. Weather conditions have a major influence on the red onion planting process until the red onions are ready to be harvested. In this study, the objective is to predict various types of weather such as rainfall, air temperature, and air humidity in seven districts in Central Java, namely Brebes, Temanggung, Demak, Boyolali, Kendal, Pati, and Tegal. To do this, the use of the LSTM (Long Short Term Memory) algorithm with its ability to store memory longer than RNN will be reliable for predicting various types of weather in the future. This research was developed with the CRISP-DM (Cross Industry Process Model for Data Mining) method which has a goal-oriented approach, this method is a mature and widely accepted method in Data Mining with various applications in Machine Learning. With the final results from 39 models by using the evaluation of the average value of train MSE 0.013, test RMSE 0.11, test MSE of 0.02, test RMSE 0.12 and succeed to predict 5 days or months ahead from the last data that is provided.

Technical Guidance on Cultivating Red Onion Plants by Applying KCL Fertilizer in North Tanjung Dayang Village, South Indralaya District, Ogan Ilir

Tanjung Dayang Utara is a village located in the South Indralaya sub-district, Ogan Ilir, South Sumatra. One of the most important inorganic fertilizers frequently used in agriculture is KCl, and considering its role in plant growth, it is crucial to increase the understanding of KCl application for plants. Organic fertilizers, such as chicken manure, are also added to the planting media to provide nutrients in addition to chemical fertilizers. The Bima Brebes variety of shallots are the crops grown in this study, and through this activity, information on shallots and fertilizer was provided, including preparing the planting media and the seedlings, planting, plant maintenance, and harvesting. This activity was carried out from July to September 2023. The activity is aimed at helping the residents of Tanjung Dayang Village comprehend the advantages, nutritional worth, and growth of shallots, as well as the proper techniques of growing shallots, particularly the Bima Brebes variety.

Combined Soil Microorganism Amendments and Foliar Micronutrient Nanofertilization Increased the Production of Allium cepa L. through Aquaporin Gene Regulation.

The aim of this study was to investigate the impact of changes in aquaporin expression on the growth of onion (Allium cepa L.) plants when subjected to dual applications of microorganism-based soil amendments and foliar nanoencapsulated mineral nutrients. Multiple physiological parameters related to water, gas exchange, and nutrient content in leaf, root, and bulb tissues were determined. Additionally, the gene expression of aquaporins, specifically PIP1, PIP2 (aquaporin subfamily plasma membrane intrinsic protein), and TIP2 (aquaporin subfamily tonoplast intrinsic protein), was analyzed. The findings revealed that the foliar application of nutrients in a nanoencapsulated form significantly enhanced nutrient penetration, mobilization, and overall plant growth to a greater extent than free-form fertilizers. Amendments with microorganisms alone did not promote growth but influenced the production of secondary metabolites in the bulbs. The combination of microorganisms and nanoencapsulated mineral nutrients demonstrated synergistic effects, increasing dry matter, mineral content, and aquaporin gene expression. This suggests that aquaporins play a pivotal role in the transport of nutrients from leaves to storage organs, resulting in the overexpression of PIP2 aquaporins in bulbs, improved water uptake, and enhanced cell growth. Therefore, the combined treatment with microorganisms and nanoencapsulated mineral nutrients may be an optimal approach for enhancing onion productivity by regulating aquaporins under field conditions.

ANALISA PEMBERIAN JENIS PUPUK ORGANIK DAN PEMASANGAN SUNGKUP TERHADAP PENINGKATAN PRODUKSI BAWANG MERAH (Allium ascalonicum L.)

Onion plant growth is often limited by nutrient availability and environmental influences. In addition, the use of hoods as a plant protection technique is expected to create microenvironmental conditions favorable to growth. The purpose of the study was to assess the effectiveness of using various kinds of organic fertilizers and the application of hoods in increasing shallot production. Factorial Randomized Group Design (RAK) was used, which consisted of two factors. The first factor was the type of organic fertilizer, namely bokashi fertilizer, guano fertilizer, and cow fertilizer. The second factor was the hood, namely control, waring hoods, and plastic hoods. Observation parameters observed in the vegetative phase consisted of: number of leaves, plant height. While the generative phase consisted of: number of tubers, wet weight of tubers per sample, wet weight of tubers per plot, dry weight of tubers per sample, and dry weight of tubers per plot. The results of variance analysis showed significant differences in the parameters of plant height at 14, 21, 28 days, and the number of leaves at 14, 21 days. In all parameters observed, the hoods treatment in the generative phase showed no significant results. The results of the study obtained that the treatment of guano fetilizer without a hoods produced the best tuber weight.

Microbial consortia as an option for biocontrol of Stromatinia cepivora

Stromatinia cepivora, the causal agent of white rot, is responsible for 60–80% of economic losses in onion and garlic crops. This work aimed to select biological control agents (BCAs) to control white rot. Ten microorganisms were tested for hyperparasitic activity on S. cepivora sclerotia in garlic (Allium sativum). Bioassays consisted of pots filled with sterile soil and 50 sclerotia in a plastic tulle bag. Four microorganisms were selected to compare their capability for degrade sclerotia on garlic. Our results showed that increasing degradation happened when Th034, Th035, Th003, and Bs006 were added to the pots containing garlic. Subsequently, three application techniques (seeds, seedlings at transplant, and seeds and transplant) were evaluated. Seven BCAs applied singly and in mixtures were evaluated in semi-field experiments for their ability to reduce white rot symptoms in onion plants in soil inoculated with 300 sclerotia per kilogram. The results indicated that efficacy was dependent on microrganism, mixture, and technique of application. The synergy factor showed that only two treatments have synergistic effects. In both cases, the mixture consisted of a strain of Bacillus and two species of Trichoderma (T. koningiopsis, T. atroviride) applied twice. In most cases, antagonistic interactions among BCAs were observed.

Effect of Spraying with an Extract of Roselle and Salicylic Acid on the Chemical Properties of Two Green Onion Cultivars (Allium Cepa L.)

A field experiment was conducted during the winter agricultural season 2022-2023 at the Agricultural Research Station of the College of Agriculture / University of Basra, Karma Ali, with the aim of studying spraying with roselle extract and salicylic acid and the interaction between them in the chemical characteristics of two varieties of green onions. The experiment included two factors, two varieties of onions (local white and local red) and seven concentrations of spraying three with roselle extract, which are (5, 10, 20) g. L-1 and three with salicylic acid (100, 150, 200) mg. L-1, in addition to the measurement treatment, by three sprays, between one spray and another, 10 days. It was carried out as a one-time split factorial experiment according to the randomized complete block design with three replications, and the medium means of the treatments were compared using the least significant difference at the level of probability 0.05. It was also found that there was no difference between the two cultivars in the content of the leaves of potassium, and the plants that were sprayed with roselle extract at a concentration of 20 mg excelled. L-1 in the aforementioned traits, except for the potassium content of the leaves, where the plants sprayed with roselle extract at a concentration of 10 mg were superior. L-1, as well as red onion plants that were sprayed with roselle extract at a concentration of 20 mg. L-1 showed significant superiority in leaves content of total chlorophyll, nitrogen, phosphorus and protein percentage, while the leaves of red onion plants sprayed with roselle extract at a concentration of 10 mg were superior. L-1 in potassium content compared to the control treatment.

Onion leaf blight caused by Alternaria alternata sensu lato and Stemphylium vesicarium in Michoacán, México

México is among the main producers of onions, which are cultivated mainly in the states of Baja California, Chihuahua, Guanajuato, Michoacán, and Zacatecas. During June and November 2017, foliar blight symptoms were observed in commercial onion crops in the municipality of Copándaro, Michoacán. The incidence of the disease was 20% in June and 80% in November. The objective of this work was to identify the causal agent or agents of this disease. Fungal isolates recovered from symptomatic plants presented conidia typical of Alternaria and Stemphylium. Molecular identification and phylogenetic analysis using partial sequences of the nuclear ribosomal internal transcribed spacer (ITS) region, the translation elongation factor-1 alpha (TEF1), and RNA polymerase subunit II (RPB2) genes confirmed the identity of the isolates as species within the Alternaria alternata species complex, and Stemphylium vesicarium. The isolates reproduced foliar blight symptoms in inoculated onion plants in pathogenicity tests. This is the first report of Alternaria alternata sensu lato and Stemphylium vesicarium causing foliar blight in commercial onion crops in Michoacán.

Chromosomes and the Mitotic Cell Cycle Phase in Onion Roots

Onions (Allium cepa) are commonly employed in vitro to evaluate the effects of chemical agents. Onion chromosomes have long been prepared using the acetic orcein staining technique. However, aceto-orcein stain is corrosive and deadly since it contains oxidizing agents like organic peroxides, toxic compounds like cyanides, acid corrosives, and radioactive materials. This study examined the mitotic activity in the roots of onion plants to ascertain the impact of the aceto orcein dye's soaking period on actively dividing root cells. Using the aceto-orcein squash procedure, a number of root tips from each bulb were collected, soaked for 1, 3, and 24 hours in aceto-orcein stain, and then processed further for cytological analyses. The purpose of the research was to examine the impact of chromosomal aberration and mitotic index on onion roots. It will reveal how many cells are actually going through mitosis. The tip of onion root cells was observed going through mitosis while actively undergoing mitotic division using the usual methods. There are various phases involved in mitotic divisions, including prophase, metaphase, anaphase, telophase, and interphase. At each soaking time, the experiment was run six times. The outcome shown that different soaking time durations had a substantial impact on the decline in mitotic index value.