The present research work aimed at developing a topical dosage form from onion extract for accelerated wound healing action. Onion extract was subjected to phytochemical screening, Gas Chromatograpgy-Mass Spectral analysis, in silico studies against selected proteins involved in wound healing process. It was assessed further for in vitro cytotoxicity and in vitro scratch assay on mouse fibroblasts cell line. Topical film forming onion extract spray formulations were prepared using Eudragit RS-100 as the film forming polymer using mixture of both stinging and non-stinging solvents. Gels were prepared using carbopol as gelling agent. Topical gels and film forming sprays were prepared and compared for wound healing. Gel formulations prepared with carbopol-940 and hydroxy propyl methyl cellulose showed better physico-chemical properties. The developed formulations were characterized for pH, viscosity, swelling index, spreadability, drug content, density, spray pattern, spray angle, drying time, average weight per dose, drug content and in-vitro permeation studies. The invitro cytotoxicity assay revealed cytocompatibility of the onion extract with L929 cell lines and possessed significant wound healing potential at concentration of 0.062 %v/v when subjected to in vitro scratch wound healing assay.