Ongoing Studies Research Articles

Genome-wide investigation to assess copy number variants in the Italian local chicken population.

Copy number variants (CNV) hold significant functional and evolutionary importance. Numerous ongoing CNV studies aim to elucidate the etiology of human diseases and gain insights into the population structure of livestock. High-density chips have enabled the detection of CNV with increased resolution, leading to the identification of even small CNV. This study aimed to identify CNV in local Italian chicken breeds and investigate their distribution across the genome. Copy number variants were mainly distributed across the first six chromosomes and primarily associated with loss type CNV. The majority of CNV in the investigated breeds were of types 0 and 1, and the minimum length of CNV was significantly larger than that reported in previous studies. Interestingly, a high proportion of the length of chromosome 16 was covered by copy number variation regions (CNVR), with the major histocompatibility complex being the likely cause. Among the genes identified within CNVR, only those present in at least five animals across breeds (n = 95) were discussed to reduce the focus on redundant CNV. Some of these genes have been associated to functional traits in chickens. Notably, several CNVR on different chromosomes harbor genes related to muscle development, tissue-specific biological processes, heat stress resistance, and immune response. Quantitative trait loci (QTL) were also analyzed to investigate potential overlapping with the identified CNVR: 54 out of the 95 gene-containing regions overlapped with 428 QTL associated to body weight and size, carcass characteristics, egg production, egg components, fat deposition, and feed intake. The genomic phenomena reported in this study that can cause changes in the distribution of CNV within the genome over time and the comparison of these differences in CNVR of the local chicken breeds could help in preserving these genetic resources.

HCC-Related lncRNAs: Roles and Mechanisms.

Hepatocellular carcinoma (HCC) presents a significant global health threat, particularly in regions endemic to hepatitis B and C viruses, and because of the ongoing pandemic of obesity causing metabolic-dysfunction-related fatty liver disease (MAFLD), a precursor to HCC. The molecular intricacies of HCC, genetic and epigenetic alterations, and dysregulated signaling pathways facilitate personalized treatment strategies based on molecular profiling. Epigenetic regulation, encompassing DNA methyltion, histone modifications, and noncoding RNAs, functions as a critical layer influencing HCC development. Long noncoding RNAs (lncRNAs) are spotlighted for their diverse roles in gene regulation and their potential as diagnostic and therapeutic tools in cancer. In this review, we explore the pivotal role of lncRNAs in HCC, including MAFLD and viral hepatitis, the most prevalent risk factors for hepatocarcinogenesis. The dysregulation of lncRNAs is implicated in HCC progression by modulating chromatin regulation and transcription, sponging miRNAs, and influencing structural functions. The ongoing studies on lncRNAs contribute to a deeper comprehension of HCC pathogenesis and offer promising routes for precision medicine, highlighting the utility of lncRNAs as early biomarkers, prognostic indicators, and therapeutic targets.

Risk Stratification and Management of Intermediate-Risk Acute Pulmonary Embolism.

Pulmonary embolism (PE) is the third most common cause of cardiovascular death and necessitates prompt, accurate risk assessment at initial diagnosis to guide treatment and reduce associated mortality. Intermediate-risk PE, defined as the presence of right ventricular (RV) dysfunction in the absence of hemodynamic compromise, carries a significant risk for adverse clinical outcomes and represents a unique diagnostic challenge. While small clinical trials have evaluated advanced treatment strategies beyond standard anticoagulation, such as thrombolytic or endovascular therapy, there remains continued debate on the optimal care for this patient population. Here, we review the most recent risk stratification models, highlighting differences between prediction scores and their limitations, and discuss the utility of serologic biomarkers and imaging modalities to detect right ventricular dysfunction. Additionally, we examine current treatment recommendations including anticoagulation strategies, use of thrombolytics at full and reduced doses, and utilization of invasive treatment options. Current knowledge gaps and ongoing studies are highlighted.

RESULTS OF BIPHASIC CALCIUM PHOSPHATE BONE GRAFT WITH SUBMICRON-SIZED NEEDLE-SHAPED SURFACE TOPOGRAPHY AS STANDALONE ALTERNATIVE TO AUTOGRAFT ARE FAVOURABLE IN A PROSPECTIVE, MULTICENTRE, RANDOMIZED, INTRA-PATIENT CONTROLLED TRIAL

Pseudoarthrosis after spinal fusion is an important complication leading to revision spine surgeries. Iliac Crest Bone Graft is considered the gold standard, but with limited availability and associated co-morbidities, spine surgeons often utilize alternative bone grafts.Determine the non-inferiority of a novel submicron-sized needle-shaped surface biphasic calcium phosphate (BCP<µm) as compared to autograft in instrumented posterolateral spinal fusion.Adult patients indicated for instrumented posterolateral spinal fusion of one to six levels from T10-S2 were enrolled at five participating centers. After instrumentation and preparation of the bone bed, the randomized allocation side of the graft type was disclosed. One side was grafted with 10cc of autograft per level containing a minimum of 50% iliac crest bone. The other side was grafted with 10cc of BCP<µm granules standalone (without autograft or bone marrow aspirate). In total, 71 levels were treated. Prospective follow-up included adverse events, Oswestry Disability Index (ODI), and a fine-cut Computerized Tomography (CT) at one year. Fusion was systematically scored as fused or not fused per level per side by two spine surgeons blinded for the procedure.The first fifty patients enrolled are included in this analysis (mean age: 57 years; 60% female and 40% male). The diagnoses included deformity (56%), structural instability (28%), and instability from decompression (20%). The fusion rate determined by CT for BCP<μm was 76.1%, which compared favorably to the autograft fusion rate of 43.7%. Statistical analysis through binomial modeling showed that the odds of fusion of BCP<μm was 2.54 times higher than that of autograft. 14% of patients experienced a procedure or possible device-related severe adverse event and there were four reoperations. Oswestry Disability Index (ODI) score decreased from a mean of 46.0 (±15.0) to a mean of 31.7 (±16.9), and 52.4% of patients improved with at least 15-point decrease.This data, aiming to determine non-inferiority of standalone BCP<μm as compared to autograft for posterior spinal fusions, is promising. Ongoing studies to increase the power of the statistics with more patients are forthcoming.

Current Issues in Reduced-Port Gastrectomy: A Comprehensive Review.

Reduced-port gastrectomy (RPG) includes all procedures derived from various efforts to minimize surgical invasiveness, with single-incision laparoscopic gastrectomy (SILG) being the ultimate reduced-port technique. However, there are challenges related to its feasibility, oncological validity, training, and education. This review describes the current issues and challenges, as well as the future prospects of RPG for gastric cancer. Gastrectomy, which started as an open surgery, has evolved into a laparoscopic surgery. With the advancements in laparoscopic technology, SILG has been used to minimize surgical scarring. However, owing to the technical difficulties of SILG, cases involving the addition of 1 trocar or needle grasper alongside the multichannel port have also been reported. Additionally, 3-port laparoscopic gastrectomy (3PLG) using only 3 trocars is also being performed. RPG, as a concept, includes a range of approaches such as SILG, 2-port laparoscopic gastrectomy, and 3PLG. These techniques aimed to reduce the number of ports or incisions required for laparoscopic gastrectomy. Despite technical difficulties, RPGs offer numerous advantages, including minimal invasiveness, excellent cosmetic outcomes, and the potential for improved post-operative recovery, such as reduced length of hospital stay and post-operative pain. It could be considered similar to conventional laparoscopic gastrectomy, and may not be oncologically inferior. Ongoing studies, such as the KLASS 12, are required to gain further insights.

Cardiovascular Risk in Patients With Glomerular Disease: A Narrative Review of the Epidemiology, Mechanisms, Management, and Patient Priorities.

Cardiovascular (CV) disease is a major cause of morbidity and mortality for patients with glomerular disease. Despite the fact that mechanisms underpinning CV disease risk in this population are likely distinct from other forms of kidney disease, treatment and preventive strategies tend to be extrapolated from studies of patients with undifferentiated chronic kidney disease (CKD). There is an unmet need to delineate the pathophysiology of CV disease in patients with glomerular disease, establish unique risk factors, and identify novel therapeutic targets for disease prevention. The aims of this narrative review are to summarize the existing knowledge regarding the epidemiology, molecular mechanisms, and management of CV disease in patients with common glomerular disease, highlight the patient perspective, and propose specific areas for future study. The literature for this narrative review was accessed using common research search engines, including PubMed, PubMed Central, Medline, and Google Scholar. Information for the patient perspective section was collected through iterative discussions with a patient partner. We reviewed the epidemiology, molecular mechanisms of disease, management approaches, and the patient perspective in relation to CV disease in patients with glomerulopathies. Throughout, we have highlighted the current knowledge and have discussed future research approaches, both clinical and translational, while integrating the patient perspective. Patients with glomerular disease have significant CV disease risk driven by multifactorial, molecular mechanisms originating from their glomerular disease but complicated by existing comorbidities, kidney disease, and medication side effects. The current approach to risk stratification and treatment relies heavily on existing data from CKD patients, but this may not always be appropriate given the unique pathophysiology and mechanisms associated with CV disease risk in patients with glomerular disease. We highlight the need for ongoing glomerular disease-focused studies aimed to better delineate CV disease risk, while integrating the patient perspective. This is a narrative review and does not represent a comprehensive and systematic review of the literature.

Unusual or Uncommon Histology of Gastric Cancer.

This review comprehensively examines the diverse spectrum of gastric cancers, focusing on unusual or uncommon histology that presents significant diagnostic and therapeutic challenges. While the predominant form, tubular adenocarcinoma, is well-characterized, this review focuses on lesser-known variants, including papillary adenocarcinoma, micropapillary carcinoma, adenosquamous carcinoma, squamous cell carcinoma (SCC), hepatoid adenocarcinoma, gastric choriocarcinoma, gastric carcinoma with lymphoid stroma, carcinosarcoma, gastroblastoma, parietal cell carcinoma, oncocytic adenocarcinoma, Paneth cell carcinoma, gastric adenocarcinoma of the fundic gland type, undifferentiated carcinoma, and extremely well-differentiated adenocarcinoma. Although these diseases have different nomenclatures characterized by distinct histopathological features, these phenotypes often overlap, making it difficult to draw clear boundaries. Furthermore, the number of cases was limited, and the unique histopathological nature and potential pathogenic mechanisms were not well defined. This review highlights the importance of understanding these rare variants for accurate diagnosis, effective treatment planning, and improving patient outcomes. This review emphasizes the need for ongoing research and case studies to enhance our knowledge of these uncommon forms of gastric cancer, which will ultimately contribute to more effective treatments and better prognostic assessments. This review aimed to broaden the pathological narrative by acknowledging and addressing the intricacies of all cancer types, regardless of their rarity, to advance patient care and improve prognosis.

Assessing forest changes in Yok Don National Park and surrounding areas, Dak Lak province, Vietnam

Forests serve as vital ecosystems, playing a pivotal role in preserving biodiversity, maintaining air and water quality, and regulating the climate, all while delivering many benefits to humanity and serving as a critical resource for diverse industries. This study investigated changes in forest cover within Yok Don National Park and surrounding areas in Dak Lak province, Vietnam, over the period from 2001 to 2021. The primary objective was to identify decadal shifts in forest cover through the utilization of freely accessible satellite remote sensing data and geographic information systems (GIS) tools. The maximum likelihood classification technique was employed to categorize Landsat images captured in 2001, 2011, and 2021. Furthermore, we evaluated the efficacy of the Normalized Difference Vegetation Index (NDVI) and the Soil-Adjusted Vegetation Index (SAVI) based classifications in detecting forest cover changes when compared to supervised classification. Initial estimates placed forest cover at 2796.91 km2, constituting 88.44% of the total land area in 2001, which subsequently declined to 2609.97 km2 (82.53%) in 2011, and further reduced to 1843.57 km2 (58.29%) in 2021. Severely reduced forest cover is often associated with the expansion of agriculture on the forest edge; other contributing factors include logging and illegal production land. Notably, the NDVI outperformed the SAVI in detecting forest cover changes. The findings of this research are anticipated to contribute to ongoing studies and enhance the effective monitoring of this protected forest area, thus providing invaluable insights for decision-makers.

Room-temperature ferroelectric nematic liquid crystal showing a large and diverging density.

The ferroelectric nematic phase (NF) is a recently discovered phase of matter in which the orientational order of the conventional nematic liquid crystal state is augmented with polar order. Atomistic simulations suggest that the polar NF phase would be denser than conventional nematics owing to contributions from polar order. Using an oscillating U-tube densitometer, we obtain detailed temperature-dependent density values for a selection of conventional liquid crystals with excellent agreement with earlier reports. Having demonstrated the validity of our method, we then record density as a function of temperature for M5, a novel room-temperature ferroelectric nematic material. We present the first experimental density data for a NF material as well as density data for a nematic that has not previously been reported. We find that the room-temperature NF material shows a large (>1.3 g cm-3) density at all temperatures studied, notably including phases without polar order. An increase in density at phase transitions is observed. The magnitude of the increase for the intermediate-to-ferroelectric nematic (NX-NF) transition is an order of magnitude smaller than the isotropic-nematic (I-N) transition. We then probe potential consequences that may result from an elevated density through measurement of the refractive indices (no and ne). The navg of M5 is compared with 5CB and polar smectic liquid crystals. We observe how the highly polar nature of the system counteracts the effects of an increase in density. With knowledge of experimental density, we are able to derive an approximation that yields the polar order parameter, 〈P1〉, from polarisation measurements. Present results may be typical of ferroelectric nematic materials, potentially guiding material development, and is especially relevant for informing ongoing studies into this emerging class of materials.

The epigenetic signatures of opioid addiction and physical dependence are prevented by D-cysteine ethyl ester and betaine.

We have reported that D,L-thiol esters, including D-cysteine ethyl ester (D-CYSee), are effective at overcoming opioid-induced respiratory depression (OIRD) in rats. Our on-going studies reveal that co-injections of D-CYSee with multi-day morphine injections markedly diminish spontaneous withdrawal that usually occurs after cessation of multiple injections of morphine in rats. Chronically administered opioids are known (1) to alter cellular redox status, thus inducing an oxidative state, and (2) for an overall decrease in DNA methylation, therefore resulting in the transcriptional activation of previously silenced long interspersed elements (LINE-1) retrotransposon genes. The first objective of the present study was to determine whether D-CYSee and the one carbon metabolism with the methyl donor, betaine, would maintain redox control and normal DNA methylation levels in human neuroblastoma cell cultures (SH-SY5Y) under overnight challenge with morphine (100nM). The second objective was to determine whether D-CYSee and/or betaine could diminish the degree of physical dependence to morphine in male Sprague Dawley rats. Our data showed that overnight treatment with morphine reduced cellular GSH levels, induced mitochondrial damage, decreased global DNA methylation, and increased LINE-1 mRNA expression. These adverse effects by morphine, which diminished the reducing capacity and compromised the maintenance of the membrane potential of SH-SY5Y cells, was prevented by concurrent application of D-CYSee (100µM) or betaine (300µM). Furthermore, our data demonstrated that co-injections of D-CYSee (250μmol/kg, IV) and to a lesser extent, betaine (250μmol/kg, IV), markedly diminished the development of physical dependence induced by multi-day morphine injections (escalating daily doses of 10-30mg/kg, IV), as assessed by the lesser number of withdrawal phenomena elicited by the injection of the opioid receptor antagonist, naloxone (1.5mg/kg, IV). These findings provide evidence that D-CYSee and betaine prevent the appearance of redox alterations and epigenetic signatures commonly seen in neural cells involved in opioid physical dependence/addiction, and lessen development of physical dependence to morphine.

The FAIR Phase-0 Hyperon Program at HADES

Abstract. Hyperons are a unique probe to study the non-perturbative aspects of the strong interaction. At HADES, they are produced in proton or pion induced reactions at kinetic energies up to 4.5 GeV. Already in the past, HADES has shown its potential for hyperon physics, including λ polarization, λ-N interaction and measurements of the λ(1405) and λ(1520) line-shapes. The HADES detector has recently been extended with a forward detector, partly developed for the PANDA experiment, extending the acceptance for hyperon channels at forward angles. The PANDA@HADES initiative gives the opportunity for an even richer hyperon program. The current main objectives are the production of hyperon resonances, electromagnetic decays of hyperons with special focus on hyperon Dalitz decays and double strangeness production, including a λ – λ interaction study and ξ− production. First results from the ongoing studies promise a successful execution of the program. In the future, there is the possibility for a pion beam experiment with HADES, enabling further hyperon studies.

An ecosocial work model for social work education in Africa

There is a growing call for social work to contribute to the international movement towards sustainability to address the interconnected multiple ecological, social, and economic crises created by the global capitalist hegemony. This call is important for African social work given vulnerable people in Africa are primary victims of unsustainability. In this paper, we propose a model of an ecosocial work course for African undergraduate social work education. This proposal is based on our earlier review of the Bachelor of Social Work curricula of 12 universities in four African countries to determine the extent to which ecosocial content were addressed. Our review indicated the absence of an ecosocial approach in the curricula. To fill this gap, we then designed a model of an ecosocial work course based on our international experience in social work, our ongoing studies on sustainability, and relevant literature regarding Africa. We hope this model will be adopted by social work programmes in different African universities and used to shape social work students who will deal with the interconnected multiple crises in their practice. We also hope that this proposal will help African social work play a crucial role in the sustainable development of the continent. How to reference using ASWNet style: Tadesse M. E. and Obeng J. K. (2022). An ecosocial work model for social work education in Africa. African Journal of Social Work, 13(2), 57-69. https://dx.doi.org/10.4314/ajsw.v13i2.2 Visit journal website: https://ajsw.africasocialwork.net

Head and neck cancers: epidemiology, diagnosis and treatments

Introduction: Head and neck cancers (HNC) affect many subsites in the upper aero-digestive tract. The risk factors include tobacco smoking, chewing betel nut/paan, excessive alcohol consumption, hereditary factors and viruses such as human papillomavirus (HPV) and Epstein–Barr virus (EBV). Early diagnosis, lifestyle changes and HPV vaccination will improve survival. Epidemiology: Globally, recent estimates from GLOBOCAN shows there are 890,000 cases of head and neck cancers annually and 450,000 deaths annually (1). There is an increasing incidence of oral cavity, lip, buccal cancers in the developing world due to higher consumption of tobacco, alcohol and chewing areca nut. There has been an increasing incidence of some HNC due to high-risk strains of human papillomavirus (HPV) (2). Cancers caused by HPV include tonsil cancer, tongue base cancer and unknown primary cancers. The EBV causes nasopharyngeal cancers especially in the Far East. There is a decreasing incidence of some cancers caused by smoking such as laryngeal and hypopharyngeal cancers mainly due to the reduction in smoking rates in some developed countries. Overall, there is a predicted increase in HNC of up to 30% annually by 2030 (2). The HPV-associated cancers affect mainly younger males, who do not necessarily smoke or drink alcohol. The HPV is a strong prognostic factor so patients with HPV positive cancers respond better to treatment as there are less genetic mutations when compared to cancers driven by tobacco smoking. These trends are alarming with socioeconomic impacts. Diagnosis: Symptoms of HNC include difficulty swallowing, persistent sore throat, difficulty breathing, hoarseness, neck lumps, referred pain to the ear, weight loss, and systemic symptoms in advanced disease. A full ENT examination is performed of the oral cavity, oropharynx, neck and ideally flexible nasendoscopy. Ultrasound-guided FNAC or core biopsy of neck lumps are performed. Cross-sectional imaging with CT or MRI scans followed by tumour biopsies either under local or general anaesthetic. Tumour biopsies are assessed using H&E stains, and tests for HPV detection (p. 16 immunohistochemistry, in situ hybridisation or polymerase chain reaction). A PETCT scan is useful for staging cancers, identifying the origin of unknown primary cancers and for surveillance. Treatments: The head and neck cancer multidisciplinary team reviews all investigations and recommends either curative or palliative treatment options that are in every patient’s best interests. Surgery can be open, endoscopic or with the use of a robot. Microvascular reconstruction with flaps is performed as necessary to improve function and quality of life. Oncologic treatments include intensity-modulated radiotherapy (IMRT), which can be combined with chemotherapy. Immunotherapy is a second-line treatment for recurrent HNC currently. Advanced cancers can be treated with palliative intent or best supportive care especially if the patients are frail and have significant comorbidities. There are ongoing clinical trials and research studies exploring better, less-toxic treatments. Multiomics approach and artificial intelligence are exciting developments. Conclusion: There is an increasing incidence of some HNC especially cancers driven by tobacco smoking, alcohol consumption and high-risk human papillomaviruses. Smoking cessation, HPV vaccination, early diagnosis and improvements in treatments are important factors in ensuring good outcomes.

High-resolution, Mid-infrared Color Temperature Mapping of the Central 10″ of the Galaxy

The neighborhood of the Galactic black hole boasts a plethora of extended interstellar gas and dust features, as well as populations of compact (unresolved or marginally resolved) features such as the G objects. Most are well manifested in the infrared. To disentangle and characterize the infrared structure of the extended features and identify compact sources, we used 3.8 μm (L′ filter) data from the NIRC2 imager at the Keck Observatory and 8.6 μm (PAH1 filter) data from the VISIR imager at the Very Large Telescope to produce the highest-resolution mid-IR color temperature map of the inner half-parsec of the Galactic center to date. From this map, we compile a catalog of features that stand out from their background. In particular, we identify 33 compact sources that stand out against the local background temperature, 11 of which are newly identified and candidates for being members of the G object population. Additionally, we resolve and newly characterize the morphology of several known extended features. These results prepare the way for ongoing and future JWST studies that have access to a greater range of mid-infrared wavelengths and thus will allow for refined estimation of the trends of dust temperatures.

Adolescents with neuropsychiatric symptoms in the turmoil of the service system: The NEPSOS interdisciplinary study of social work, adolescent psychiatry, special education, and expert organisations

Existing social, health and education services do not respond to the needs of adolescents with neuropsychiatric symptoms. These services are usually fragmented and their professionals’ expertise is insufficient. In this interprofessional context, the integration of specialisations, multidisciplinary expertise and knowledge is a challenge. In the NEPSOS study, we explore the services aimed at and service needs of adolescents with neuropsychiatric symptoms and their families. The NEPSOS study is collaboratively designed and carried out by experts from the University of Turku and the University of Eastern Finland, including researchers of adolescent psychiatry, social work and special education, as well as expert organisations such as the Southwest Finland Centre of Excellence on Social Welfare and the Nationwide Association of Different Learners. These organisations provide their expertise for the implementation and integration of the research results into the service system and the development of recommendations. 
 In this study, the appropriateness of services and service paths are examined from the perspectives of adolescents and families, professionals and interprofessional collaboration. The study has four different lines. Line 1 examines the relationships between a) the socio-economic status of the parent and the situation of the adolescent and b) neuropsychiatric symptoms and child protection support measures. Line 2 examines the experiences and views of adolescents and their parents concerning the functionality of the service system and the functional forms of psychosocial support. Line 3 studies the professionals’ perspectives on the functionality of the service system and interprofessional collaboration in the context of adolescent service paths. Line 4 focuses on communication and interaction activities, including the implementation of the research results.
 The NEPSOS study will provide knowledge and deepen the understanding of the following topics:
 •How interprofessional cooperation is implemented in the service paths for adolescents with neuropsychiatric symptoms from the perspective of professionals from the fields of social work, adolescent psychiatry and education.
 •How existing service networks meet the needs of adolescents and their families, and what kind of psychosocial support is provided to them by the professionals of social work, adolescent psychiatry, and special education. 
 •How psychosocial services and interprofessional collaboration are performed to improve the timeliness, targeting and integration of services for adolescents with neuropsychiatric symptoms and their families.
 Furthermore, the impact of this study’s findings will be manifested as practical recommendations and training materials for professionals. The NEPSOS study forms part of an interdisciplinary research consortium whose ongoing and future studies specifically aim at strengthening the integration of social care, health care and education for the health and wellbeing of children and families. Our experiences regarding interdisciplinary collaboration and the future results of the study can be applied internationally both in higher education and the integration of child social, health and education services.
 Keywords: neuropsychiatric symptoms, adolescents, interdisciplinary collaboration, social work, adolescent psychiatry, special education.

Characterizing oceanographic conditions near Coiba Island and Pacific Panama using 20 years of satellite-based wind stress, SST and chlorophyll-a measurements

Coiba Island and the associated Special Zone of Marine Protection represent an important, yet poorly studied marine reserve along the Pacific coast of Panama. While efforts have recently began to establish monitoring programs in the region, a range of historical, marine-related environmental measurements already exist, derived from satellite-based observations. The goal of this paper was to use long-term datasets for key variables to provide qualitative insights (i.e. descriptive oceanography) of climatological conditions and interannual variability in the Pacific Panama region. These are underpinned with numerical assessments, providing an important baseline for ongoing and future studies, particularly in the Coiba Island/Gulf of Chiriqui region. In particular, we examined 20 years (January 2003-December 2022) of wind stress, sea surface temperature (SST), and chlorophyll-a (Chl-a), spanning the neritic and pelagic regions of the Pacific Panama coast. During the dry season (northern winter), the well-known, seasonal, regional Panama wind jet appeared across the Gulf of Panama, leading to surface mixing and SST cooling that eventually extended across most of the Panama Bight. West of the Azuero Peninsula, SST increased and surface warming extended further offshore from January through April. The SST in the Gulf of Chiriqui during this period was about 1 °C warmer on average than east of Coiba Island. By July and August, offshore SST gradients became largely longitudinal, cooling occured across the season, and the SST on either side of Coiba Island was nearly the same. The influence of the Panama jet in the Gulf of Panama was clear in the Chl-a data as well, with upwelling-driven values peaking in February/March (up to 11 mg m-3, with a monthly climatological value of around 2 mg m-3 during this period). During the rest of the year, the Chl-a concentration in this region averaged around 0.5-1.0 mg m-3. In the Gulf of Chiriqui and the region east of Coiba Island, the climatological monthly averages were roughly 0.3-0.5 mg m-3 and 0.4-0.6 mg m-3, respectively. Somewhat surprisingly, very high Chl-a values were present in the satellite data for the Gulf of Chiriqui during May 2007 and June 2008, peaking at 16 mg m-3 and 32 mg m-3 at a location just west of Coiba Island, respectively. It remains unclear as to the cause of these apparent blooms. Even when the high Chl-a values were excluded in the calculation of climatological averages in the Gulf of Chiriqui, however, there is a suggestion of modest seasonality in Chl-a values, with slightly elevated values (~ 0.4 mg m-3) peaking around May and October. During the extreme El Niño event of 2015-2016, the monthly-averaged SST along the Panama Pacific coast was warmer than average, with elevated levels of up to + 2 °C and lasting 12 months in the Gulf of Chiriqui. In the Gulf of Panama, the monthly-averaged SST anomalies were up to + 1.7 °C, although the temperatures returned to near-seasonal averages after roughly 5 months.

Antimicrobial approach of abdominal post-surgical infections.

Abdominal surgical site infections (SSIs) are infections that occur after abdominal surgery. They can be superficial, involving the skin tissue only, or more profound, involving deeper skin tissues including organs and implanted materials. Currently, SSIs are large global health problem with an incidence that varies significantly depending on the United Nations' Human Development Index. The purpose of this review is to provide a practical update on the latest available literature on SSIs, focusing on causative pathogens and treatment with an overview of the ongoing studies of new therapeutic strategies.

Towards a praxis of care in post-pandemic fieldwork: Comparing ethnographic encounters during Covid-19

The Covid-19 pandemic has highlighted the importance of ensuring the wellbeing of both researchers and participants throughout the research process. In this paper, we argue that in order to produce caring research, the wellbeing of researchers must not be neglected. Using our experiences as three doctoral researchers undertaking ethnographic fieldwork during the pandemic, we consider what it means to do research which has a ‘praxis of care’ at its core. By consciously embedding care into the research process, we argue that we can work towards research that prioritises multiple modes of care and compassion. To demonstrate this, we present and reflect on our three related, but individual experiences as ongoing case studies. Grounding our own research encounters within broader literature focused on self-care in early career research alongside feminist perspectives, we ask the following questions: In the pursuit of knowledge, what does taking a step back to care for ourselves look like? How can we plan fieldwork which operates without harm for both researcher and participant? Finally, we contemplate what fieldwork with an epistemological commitment to ‘care’ for both researchers and participants could look like and propose some practical recommendations for incorporating a praxis of care throughout the research process.

Imperative Role of Natural Product Chemistry in Cosmeceutical R&D - Phytonanocosmeceuticals

Natural product chemistry has always been attractive for drug and cosmetic industries as natural products can address these industries very well. By consumer demand, the cosmetic industry is looking for innovative, safer, more effective, and environmentally friendly products. In this sense, a relatively new concept of cosmetics has emerged under “cosmeceuticals or dermocosmetics/medcosmetics”. Cosmeceuticals are briefly defined as a subclass of cosmetics that contain drug active substances or bioactive natural products with enhanced efficacy for therapeutic or cosmetic purposes. They are also described as a combination of cosmetics and pharmaceutics or medical-grade cosmetics, which particularly enhance skin penetration and the restorative effect of active ingredients in cosmetic formulations. On the other hand, nanotechnology has become another exciting area in cosmetics as nanoformulations enhance skin penetration. Therefore, we have been working on research and development of novel phyto-based cosmeceuticals via extensive screening studies on plant extracts and pure natural substances using in vitro (enzyme inhibition, etc), in silico (molecular docking and toxicity screening), and cell-based assays. In this regard, an anti-acne formulation based on a number of plant extracts tested against Propionibacterium acnes has been developed by our group. Besides, an antimicrobial formulation as an oral spray for mouth defense is currently a commercial product. We have been studying nanofiber formulations loaded with plant extract for wound healing. All our ongoing studies on discovering novel active natural ingredients for cosmeceutical purposes have so far yielded three patents and four patent applications and commercialized a final product. In the present mini review, examples and details of our group’s recent findings on phyto-based cosmeceuticals and nanoformulations will be underlined.

Navigating the Complexity: A Comprehensive Review of Managing Pregnancy in Complete Heart Block Cases.

This review explores the comprehensive management of pregnancy in cases of complete heart block, a cardiac condition characterized by the dissociation between atrial and ventricular conduction. The review begins with a thorough examination of preconception counseling, emphasizing the assessment of maternal health, the evaluation of cardiac function, and the identification of potential risks. Subsequently, the medical management section delves into using beta-blockers, pacemakers, and antiarrhythmic drugs to monitor cardiac function during pregnancy and adjust medication regimens. Obstetric considerations highlight the importance of antenatal care, fetal monitoring, and thoughtful delivery planning, including the choice between vaginal delivery and cesarean section. The section on complications underscores the risks of arrhythmias, heart failure, premature birth, and neonatal cardiac issues. Looking ahead, the future directions and research section explores ongoing studies in genetics, pharmacology, and technological innovations, envisioning potential advancements in pacing technology and personalized medicine approaches. The conclusion synthesizes key findings, offering recommendations for clinical practice and reflecting on the challenges and opportunities inherent in managing pregnancy in complete heart block cases. The multidisciplinary approach emerges as paramount, with collaborative efforts paving the way for improved patient outcomes and advancements in the field.