One-way ANOVA Statistical Test Research Articles

A Dosimetric Comparison Study for Blood Irradiation Employing Different Medium and Algorithms in Clinical Linear Accelerator.

To identify a suitable approach for blood irradiation other than the commonly used water medium and to study the impact of different algorithm dose computations. Water is the commonly used medium for blood irradiation. In this study computed tomography scans were taken with locally made blood irradiation phantoms other than water, by using air, rice powder and thermocole using parallel beam for 25 Gy. Plans were recalculated for different algorithms such as collapsed cone (CC), Monte Carlo (MC) and pencil beam (PB). The dose-volume parameters and measured doses were collected and analyzed for each medium and algorithm. The monitor unit (MU) for rice powder and water are close (2461±57 and 2469±61, respectively), with a maximum dose of 28.0±1.8 and 28.0±1.9 Gy. The PB algorithm resulted in lower monitor unit values regardless of the medium used, generating values of 2418, 2406, 2382, and 2362 for water, rice powder, air, and Thermocol, respectively. A significant increase in dose was observed irrespective of the medium used when the MC algorithm was employed, with a maximum of 30.26 Gy in rice powder; a smaller dose was used when the CC algorithm was employed, with 26.3 Gy in water medium. The average maximum doses of all groups were equal using the one-way Anova statistical test. Regarding the impact of field size, rice powder appears to have consistent doses across various field sizes, with slight increases as field size grows, which is similar to water. While water is the conventional medium, this study highlights the potential benefits of rice powder, such as eliminating the risks associated with bubble formation and water spillage, which can lead to equipment malfunction and safety hazards. Although previous studies have explored rice powder as a bolus and tissue-equivalent material, this study uniquely applies this knowledge to blood irradiation, an area where rice powder has not been thoroughly investigated.

Evaluation of Etching Time Effect on Shear Bond Strength of Orthodontic Brackets Bonded with Self-Adhesive Composite: An in-Vitro Study

Introduction: Precise acid etching of the tooth surface is a significant factor in the strength of the bracket bond. The current study aimed to determine the effect of different application times of acid etching on the shear bond strength of metallic orthodontic brackets under controlled laboratory conditions. Methods: Sixty intact premolar teeth were selected for this in-vitro study. The samples were divided into four groups based on the different acid etching times: 20, 25, 30, and 35 seconds. The teeth surfaces were etched with 37% phosphoric acid according to the specified times, and brackets were bonded to the enamel surface using GC Ortho connect composite. Shear bond strength values of the brackets was measured using a Universal Testing Machine. The amount of Adhesive Remnant Index was also observed using a stereomicroscope. Additionally, the surface roughness of the tooth enamel after bracket bonding was examined using SEM. The results related to shear bond strength were analyzed with one-way ANOVA statistical test and using SPSS version 16 software. The non-parametric Kruskal Wallis (Wallis-Kruskal) test was also used to check the frequency of ARI index. Results: The highest shear bond strength was observed at 25 seconds, and the lowest at 30 seconds. However, there was no significant difference in the shear bond strength of brackets was observed among the four groups (p=0.186). The highest ARI score was observed at 35 seconds, and the lowest at 20 seconds. No statistically significant difference was observed in the ARI scores in different groups. SEM images revealed that the enamel surface porosity was highest at 20 seconds. Conclusion: The results indicate that the shear bond strength and ARI at different acid etching times do not significantly differ. Therefore, orthodontic bracket bonding can be performed with less acid etching time.

The Effect of a Combination of Ethanol Extract from Leaves and Flowers of Plumeria acuminate L. Against Aedes aegypti Larvae

Aedes aegypti is the primary vector that causes dengue virus (DENV) infection. Ae. aegypti resistance to abate (Temephos) has occurred in Indonesia and several other countries. Innovations are needed to develop alternative ingredients that can be used as natural larvacides. The Plumeria acuminate L. is an Indonesian plant with metabolites that have the potential to act as larvicides. This study aims to determine the potential combination of ethanol extract of P. acuminate L. leaves and flowers as a larvicide for A. aegypti. A laboratory experimental study with a post-test-only control group design was carried out on Ae. aegypti larvae instar III. The larvicide test was carried out according to WHO standards in 2005 with concentrations of 10000, 7500, 5000, and 2500 ppm, as well as control (water and 1% DMSO), with 20 larvae for each concentration. The observation was carried out at the 24th and 48th hours with 3 repetitions. Larval mortality data was analyzed using one-way ANOVA statistical tests to determine significant differences and lethal concentrations (LC50 and LC90) were calculated using probit analysis using SPSS software. The mortality of Ae. aegypti larvae due to the administration of a combined ethanolic extract of P. acuminate L. leaves and flowers was the highest at doses of 10000 and 7500 ppm, which was 100% with lethal concentrations of LC50 and LC90 of 3364.715 and 6293.759 ppm at the 24th hour observation, whereas at the 48th-hour observation, lethal concentrations were detected at 1767.998 and 2941.138 ppm, respectively. One-way ANOVA analysis test showed a significant difference in Ae. aegypti larval mortality due to the administration of a combined ethanol extract of P. acuminate L. leaves and flowers at 24th-hour observation (p = 0.000) and (p = 0.013) at 48th hour observation. The combination of ethanol extract of P. acuminate L. leaves and flowers showed larvicidal activity against Ae. aegypti larvae as evidenced by larvae mortality which were influenced by concentration and observation time.

Administration of low dose intranasal ketamine exerts a neuroprotective effect on whole brain irradiation injury model in wistar rats.

Exposure to ionizing radiation leads to oxidative stress and neuroinflammation, resulting in neurocognitive impairments. Adverse effects are also associated with glutamate-induced excitotoxicity due to alterations in the composition of glutamate receptors. Ketamine, which is a noncompetitive NMDA glutamate receptor antagonist, has been stated to exert an impact on glutamatergic receptors. This study aims to reveal the possible alleviating or preventive effects of ketamine, which maintains glutamate homeostasis and decreases neurodegeneration, in a radiation-induced neurotoxicity model. Twenty-one female Wistar Queryrats were included in the study and 14 of these underwent whole brain irradiation (IR) with a 20 Gray single dose. Animals were allocated into three groups. Group 1: Normal control; Group 2: Placebo / IR + Saline; Group 3: IR + Ketamine. Ketamine was administered in addition to IR to rats in Group 3. The one-way ANOVA statistical test was used to compare groups. The value of p < 0.05 was considered statistically significant. When administered in addition to irradiation, ketamine treatment significantly increased scores in the three-chamber sociability test, open field test, and passive avoidance learning test. It also raised neuron counts in the hippocampal CA1 and CA3 regions as well as in Purkinje cells, and enhanced levels of brain-derived neurotrophic factor and tyrosine receptor kinase-B. Furthermore, ketamine administration resulted in decreased levels of glial fibrillary acidic protein, malondialdehyde, and tumor necrosis factor-alpha, indicating a reduction in neuroinflammation and oxidative stress. Ketamine exerted a significant protective impact on radiation-induced neurocognitive impairments and enhanced social-memory capacity by reducing neuronal loss, oxidative stress, and neuroinflammation. Our findings suggest that ketamine is beneficial in the treatment or prevention of neurodegeneration via the regulation of the BDNF/TrkB signaling pathway besides decreasing neuroinflammation and blocking NMDA receptors.

Antibacterial activity of jamblang leaf ethanol extract (Syzygium cumini) against the growth of Propionibacterium acnes

Background: Propionibacterium acnes is a bacterium found on the skin that plays a significant role in acne vulgaris. The inappropriate use of antibiotics can lead to resistance, necessitating the search for alternative therapies from plants with high antibacterial potential. One such plant is jamblang (Syzygium cumini), which has demonstrated antibacterial properties. Objective: This research aims to test the antibacterial activity of jamblang leaf extract against the growth of P. acnes. Method: The study employed an experimental design with a posttest-only control group design. The treatment groups consisted of a positive control (clindamycin), a negative control (sterile distilled water), and jamblang leaf extract groups with concentrations of 100%, 50%, 25%, 12.5%, and 6.25% (n=4). Data analysis was conducted using the One-Way ANOVA statistical test. Results: The jamblang leaf extract exhibited antibacterial potential against the growth of P. acnes. The extract showed strong antibacterial activity at concentrations of 100%, 50%, 25%, and 12.5%, and moderate antibacterial activity at a concentration of 6.25%. Conclusion: This suggests that jamblang leaf extract could be a promising alternative therapy for treating acne vulgaris, offering a natural solution to combat antibiotic resistance.

The relationship between physical activity and Body Mass Index among medical students

Preserving one's physical health and preventing diseases is of utmost importance, and leading a healthy lifestyle is essential. The absence of physical activity has been identified as a significant risk factor for obesity and chronic illnesses.This study aimed to establish an intricate relationship between physical activity level and Body Mass Index (BMI) among medical students. This analytical study used a cross-sectional design. Data were collected from October to December 2023. A total of 144 medical students enrolled at Universitas Prima, Indonesia participated in this study. Physical activity data were obtained using the International Physical Activity Questionnaire (IPAQ), whereas BMI was measured using the standard method of dividing weight in kilograms by the square of height in meters (kg/m²). Data analysis was performed using a One-Way ANOVA statistical test. The study findings revealed a significant difference (p &lt; 0.05) between physical activity levels and BMI. Students in the light activity group had an average BMI of 28.57 kg/m², compared to 23.27 kg/m² for the moderate activity group and 21.66 kg/m² for the vigorous activity group. This indicates that physical activity plays a crucial role in determining BMI, with moderate- and vigorous-intensity activities associated with ideal BMI ranges. This study underscores the importance of physical activity in maintaining healthy weight and reducing the risk of obesity and associated chronic diseases. Promoting physical activity among university students can be an effective strategy for promoting their overall well-being.

Comparison of Carbon Storage in Forested and Non-Forested Soils in Tropical Wetlands of Caimanera, Colombia, and Llano, Mexico

Mangrove forests are considered to be the most productive ecosystems on the planet due to the multiple ecosystem services they offer, both environmental economic, and social; however, their area in recent years has been decreasing due to anthropogenic activities such as urbanization and deforestation. These activities alter the normal cycle of carbon stored in sediments, which is considered their main function to counteract climate change. Therefore, the objective of this research was to compare the carbon storage capacity and coverage in forested and non-forested sites of two tropical wetlands located in Colombia (the Caimanera) and Mexico (the Llano). Methodologically, we began by identifying the coverages and determining the area in the wetlands with Sentinel-2A satellite images and a supervised classification; subsequently, soil cores were extracted in all the coverages to a depth of 70 cm and the apparent density (AD), the percentage of organic carbon (OC), and the density of carbon in the soil were determined. For analysis of the variables, a trend graph was constructed between carbon density and depth with descriptive statistics, using one-way ANOVA to establish which coverage and wetland were the most significant concerning carbon storage. The results of the supervised classification showed that Rhizophora mangle and Avicennia germinans are the dominant species, also finding deforested areas in both wetlands. The one-way ANOVA statistical test indicated that the Caimanera, with the percentage of organic carbon (18.4 ± 1.19%), is 1.57 times greater than of the Llano mangrove swamp with (11.7 ± 0.748 MgC/ha). Through the trend graph, it was observed that the carbon density of the forested area of the Caimanera ranged from 120 to 140 MgC/ha, which is higher than in the deforested areas of the same wetland between 40 and 60 MgC/ha, and the homologous areas of the Llano wetland. The results suggest that deforested areas are sources of greenhouse gas emissions because they contain less carbon than forested areas. Therefore, it is concluded that the Caimanera and the Llano wetlands have the same mangrove species and that the average organic carbon stored in their soil is below the average of other mangrove forest soils in the American continent; it is recommended that the findings of this work be considered for the carbon balances by continent and the characterization of mangrove species according to their carbon storage capacity.

Formulation and Characterization of New Experimental Dental Composites with Zirconium Filling in Different Forms.

Short glass fibers are generally used in posterior dental restorations to enhance the mechanical properties and improve the material microstructure. Two resin-based composites (S0 and SF) were formulated and characterized to investigate the influence of zirconium in their characteristics and properties. The organic part of the investigated materials was the same (BisGMA, TEGDMA, and a photochemical polymerization system), and in the inorganic part, besides quart, glassA, and hydroxylapatite with Zn, sample S0 contained strontium glass with zirconium and sample SF contained fiber powder of chopped zirconium. The samples were characterized by the degree of conversion (DC), mechanical properties, water sorption (WS), scanning electron microscopy (SEM), atomic force microscopy (AFM) before and after the WS test, and antimicrobial properties. The results obtained were subjected to one-way ANOVA and Tukey's statistical tests. Both samples had a high DC. Regarding the mechanical properties, both samples were very similar, except DTS, which was higher for the composite without fibers. After 14 days, the WS value of the SF sample was lower than that of the S0 sample. Water caused significant changes in the topography of the SF sample, but thanks to its antimicrobial properties and the diffusion phenomenon, SF had a more pronounced antimicrobial effect. This study shows that the addition of appropriate amounts of Sr-Zr-glass powder gives the material in which it is added similar properties to material containing chopped zirconium glass fiber powder. According to the antimicrobial test results, resin composites containing experimental zirconia fillings can be considered in future in vitro clinical studies for posterior reconstructions with significantly improved mechanical properties.

The Impact of Butterfly Pea Flower (Clitoria ternatea L.) Extract on Atherosclerosis Biomarker Profiles in Obese White Rats (Rattus norvegicus L.)

Background: Clitoria ternatea L., commonly known as butterfly pea flower, has gained attention as a potential agent in the prevention and treatment of atherosclerosis due to its rich content of bioactive compounds, such as anthocyanin, a type of flavonoid renowned for its potent antioxidant and anti-inflammatory properties, along with its ability to enhance the immune system.Objective: This study aimed to elucidate the positive impact of butterfly pea flower extract on atherosclerosis biomarker profiles.Methods: The approach framework used in this research is a true experimental laboratory with a Control Group Post-Test design. Obese male white rats were selected as the research subjects. Thirty-six obese white rats were randomly divided into six groups using the Completely Randomized Design (CRD) method. The data collected were VCAM and IL-6 levels from the blood serum of obese white rats tested using an ELISA photometer. The tunica intima thickness was measured using a microscope. Data were analyzed employing SPSS 18 software, utilizing one-way ANOVA statistical tests and post hoc Tukey tests.Results: The research showed that with the increase in Butterfly Flower Extract (BPFE) dose, there was a consistently lower level of inflammatory biomarkers, such as VCAM-1 and IL-6, compared to positive controls and other variations, as well as tunica intima thickness was thinning than others. A dose of 600 mg/kg BW (P3 group) had VCAM-1 levels up to 30.40 ± 6.71 ng/mL, IL-6 levels up to 17.70 ± 8.29 ng/mL, and tunica intima thickness up to 3.18 ± 1.24 µm.Conclusion: BPFE effectively lowers inflammatory biomarkers and thins the tunica intima thickness in obese white rats at 600 mg/kg BW dose. It may offer promising therapeutic potential in addressing atherosclerosis.

Potential Pharmaceutical Biotechnology Products in the Form of Formulations and Preparations of Telang Flower Kombucha Dish Soap (Clitoria ternatea L) as an Antibacterial from Beef (Bos taurus) Isolate

This research aims to see the potential of each formulation and preparation of dishwashing soap with the active ingredient telang flower kombucha in inhibiting the growth of pathogenic bacteria isolated from beef. This research is a laboratory experiment, namely by making a dishwashing soap base without the active substance of telang flower kombucha. Make a dishwashing soap base that adds the active substance of butterfly pea flower kombucha at concentrations of 20%, 30% and 40%. Biochemical identification results have shown that beef samples contain gram-positive bacteria in the form of Staphylococcus aureus and Listeria monocytogenes, as well as gram-negative bacteria in the form of Salmonella thyposa and Escherichia coli. The results of this research have also proven that pharmaceutical biotechnology products in the form of formulations and dishwashing soap preparations with the active ingredient telang flower kombucha have a positive correlation in inhibiting the growth of the four test bacteria. Based on the one-way ANOVA statistical test at each P value &lt;0.05, it proves that telang flower kombucha dish soap at a concentration of 40% according to post hoc analysis is not significantly different from concentrations of 20% and 30% as a pharmaceutical and antibacterial biotechnology product. isolated from beef. The conclusion of this research is that the formulation and preparation of dishwashing soap at a concentration of 40% proved to be the most effective as a pharmaceutical biotechnology product in inhibiting the growth of the four test bacteria.

Effect of pH Variations in Eosin Methylene Blue Agar (EMBA) Medium on E. coli Growth

The growth of E. coli is influenced by several factors, including environmental pH. Environmental pH unsuitable for bacterial growth conditions will interfere with the enzyme activity and influence bacterial growth. This study aimed to determine the effect of pH variations (5.3, 5.8, 6.3, 7.3, 7.8, 8.3) in EMBA medium on E. coli growth. The research method used was true experiment post-test-only control design. The samples in this study were suspensions with dilutions of 10-4, 10-5, 10-6, and 10-7, inoculated using the duplo test to EMBA with various pH conditions, so that the total sample size is 48. The results showed that the mean number of E. coli bacteria on EMBA with pH 5.3 was 9.1 x 106 CFU/mL; with pH 5.8 was 9.6 x 106 CFU/mL; with pH 6.3 was 1.2 x 107 CFU/mL; with pH 7.3 was 1.1 x 107 CFU/mL; with pH 7.8 was 9.7 x 106 CFU/mL; and with pH 8.3 was 7.1 x 106 CFU/mL. Growth in positive control showed the mean number 1.4 x 107 CFU/mL; negative control showed no growth of E. coli or other microorganisms. Based on the One-Way ANOVA statistical test with a 95% confidence level, there was no difference in the mean number of E. coli bacteria in the six pH variations of EMBA medium (p-value &gt; 0.05). E. coli bacteria grew best at neutral pH. Its growth decreases in slightly acidic and slightly alkaline pH, but it can still be observed. This allows E. coli to survive in extreme pH. Pathogenic E. coli have developed the potential to live inside the human body. They will experience temporary stress in unfavourable conditions before finally adapting. The advice for future researchers is to test the effect of pH on E. coli growth by using other E. coli growth media or with a wider range of pH (more acidic and more basic). It is also recommended to conduct further research about the effect of various environmental conditions such as temperature, nutrients, and others on bacterial growth.

Total Flavonoid Content and In Vitro Anti-Inflammatory Potentials of Kombucha with Enrichment of Butterfly Pea (Clitoria ternatea) Flower Extract

Kombucha is a fermented beverage from tea (Camellia sinensis) that is claimed to be beneficial for several medical ailments. Several studies have conducted nutritional enrichment of kombucha to optimize its nutritional benefits. One of the native Indonesian plants that can be utilized as nutritional enrichment material is the butterfly pea (Clitoria ternatea) flower. Its nutrition is expected to increase the health benefits of kombucha Camellia sinensis. Kombucha has beneficial compounds such as flavonoids that have potential applications in medical chemistry due to its anti-inflammatory properties. This study aims to enrich the valuable nutrition and test the in vitro anti-inflammatory potentials of kombucha, conducted by formulating kombuchaCamellia sinensis with Clitoria ternatea flower extract addition (0; 0.5; 1; 1.5; 2)% (m/v), the chemical characterization including pH value test using digital pH meter and total flavonoid content (TFC) using colorimetric (AlCl3) assay) with quercetin as its standard, and in vitro anti-inflammatory activity test using the red blood cell (RBC) membrane stabilization method. The result showed that the pH value of kombucha had met the consumption quality standard (3-4), starting from 3,30 + 0,00 (0%) to 3,66 + 0,01 (2%). The flavonoid content increased from 38,89 + 0,05 mgQE/g (0%) to 126,73 + 0,13 mgQE/g (2%). Kombucha samples showed anti-inflammatory activity potentials with the best IC50 value of 140,22 ppm (2% addition). The data from this study were analyzed using a one-way ANOVA statistical test. It shows there is an influence from the addition of Clitoria ternatea to the TFC result (p&lt;0,05), and it continued to Duncan's Multiple Range test (DMRT) that showed sample F0 to F4 were significantly different from each other (p&lt;0,05). In conclusion, the enrichment of kombucha (Camellia sinensis) using Clitoria ternatea flower extract has been proven to increase the health-beneficial compound (flavonoids) and have potential anti-inflammatory activity.

Aloin Extract on Histopathological Features of Osteogenesis in Fractures of Rattus Norvegicus Wistar Strains

The process of bone repair after trauma or fracture will involve cells, signalling molecules, and processes that have been active during the remodelling process. The new study found that Aloin can promote the expression of osteogenic differentiation regulators, such as Bmp-1 (Bone morphogenetic protein 1) and Runx1 (Runt-related transcription factor 1) in MC3T3–E1 cells, where Runx1 is a transcription factor in osteogenic differentiation—knowing and analyzing aloin extract on the histopathological picture of osteogenesis in Wistar strain rattus norvegicus fractures. This type of research is a laboratory experimental test with a post-test-only control group design. The population number will be divided into four groups, namely positive control group (+), negative control (-), treatment group 1, and treatment group 1, with 14 samples using the Purposive Sampling technique. Univariate data analysis is presented as mean and standard deviation and one-way ANOVA statistical test. Data processing using computerized IBM version 15.0 of the SPSS program. Histopathological picture of osteogenesis in fractures of male rats (Rattus norvegicus) based on osteoblast values at least at K(-), which is 30.64, and K(+), which is 31.90. In the P1 group, the highest average osteoblast value was 39.58, and P1's was 38.14. The osteoclast value is obtained at least at K(-) and 8, and K(+) is 19.01. In the P1 group, the highest average osteoclast value was 16.73, and P1 was 11.70. Aloin extract did not affect the histopathological picture of osteogenesis in fractures of male rats (Rattus norvegicus) with a sig of &gt;0.05.

First Report of Binucleate Rhizoctonia AG-G Causing Grapevine (Vitis Vinifera) Trunk Diseases in California Nurseries.

Grapevine Trunk Diseases (GTD) are caused by a consortium of fungal pathogens that affect the biological functions of the vascular system of mature and young grapevines (Gramaje et al. 2018). We conducted surveys to profile GTD pathogens in California grapevine nurseries and collected 784 cuttings of cvs. Cabernet Sauvignon and Chardonnay grafted on 1103P rootstock. Several vines exhibited wood necrotic lesions and cankers at the graft union and the root ball (Figure 1A). Symptomatic wood tissues were cultured on PDA medium and after two weeks of incubation at room temperature (22°C), several known GTD pathogens were recovered. We also identified Rhizoctonia from 42 of the 784 vines (5.3% incidence) based on the morphological characteristics of a brown pigmented mycelium (Figure 1B), hyphae branched at a right angle with constrictions at the branch point (Figure 1C) and absence of spores (González García et al., 2006). A subsample of four isolates (DCHG2B, DCSG22R, JCSG9B, and JCHG12B) were randomly selected for further DNA-based taxonomic identification and pathogenicity evaluation to grapevine. The ITS and beta tubulin regions were amplified using the ITS1/ITS4 and B36F/B12R primer sets, respectively (González et al. 2006), and sequences were deposited in the NCBI database (Accession numbers: OR052655, OR052656, OR052657, OR052658 and OR059207, OR059208, OR059209, OR059210). Sequences displayed >99% and >96% identity with the respective ITS and beta tubulin sequences of the binucleate Rhizoctonia AG-G specimen C-653 (González et al. 2006). A phylogenetic tree constructed using the Neighbor-Joining method indicated a 100% bootstrap support with the binucleate Rhizoctonia AG-G (Figure 2). Pathogenicity of the binucleate AG-G Rhizoctonia were confirmed on two separate technical replicates using standard methods. For each replicate, one-year-old rootstock 1103P were wounded with sterile drill bits and inoculated with a single 5 mm diameter agar plug collected from Rhizoctonia growing cultures, while control vines were inoculated with sterile agar. The first replicate lasted 28 weeks with (DCHG2B, DCSG22R) inoculated on seven vines. The second bioassay lasted 24 weeks with two additional isolates (JCSG9B, JCHG12B) inoculated on twelve vines. Rhizoctonia-inoculated vines developed wood symptoms similar to those observed on cuttings in nurseries, with necrotic lesions lengths significantly longer than the controls (First replicate: 3.5  0.4 cm vs. 1.3  0.6 cm; Second replicate: 6.8  0.8 cm vs. 1.1  0.2 cm), based on one-way ANOVA statistical test (P value < 0.05). Rhizoctonia isolates recovery from wood necrotic lesions were confirmed by ITS sequencing, thereby fulfilling Koch's postulate. Several binucleate Rhizoctonia anastomosis groups, including AG-G, have been found to cause root rot and stem necrosis in plant nurseries (Aiello et al., 2017; Rinehart et al., 2007). Rhizoctonia has also been reported to be associated with grapevine nurseries in Europe (Pintos et al., 2018), South Africa (Halleen et al., 2003) and Australia (Walker, 1992). However, the multinucleate Rhizoctonia solani was the only species confirmed to cause root rot on grapevine (Walker, 1992). Our data suggests that the binucleate Rhizoctonia from the AG-G anastomosis group also cause wood necrosis in grapevine. Those findings warrant further studies on the complexity of Rhizoctonia anastomosis groups in nursery and their aggressiveness to grapevine.

Uji Aktivitas Antifungi Kombinasi Ekstrak Etanol Kulit Pisang Kepok (Musa paradisiaca L.) dan Kulit Jeruk Manis (Citrus sinensis) terhadap Candida albicans Penyebab Keputihan Patologis Secara In Vitro

Pathological vaginal discharge (Candidiasis) is a reproductive health problem experienced by more than 50% of women throughout the world. Candida albicans is the main cause of pathological vaginal discharge. This disease is characterized by yellow to greenish discharge, odor, and itching to the point of bleeding which can cause death. Antifungal drugs used to treat pathological vaginal discharge can have several side effects. Therefore, the urgency of this research is to find natural alternatives for pathological vaginal discharge drugs, such as sweet orange peel and kepok banana peel which contain bioactive compounds that can inhibit the growth of Candida albicans. The aim of this study was to test the antifungal activity of a combination of ethanol extracts of sweet orange peel and kepok banana peel against Candida albicans in vitro. This research uses laboratory experimental methods. The results showed that all combinations of ethanol extracts of sweet orange peel and kepok banana peel could inhibit the growth of Candida albicans. The inhibitory power of each extract combination is categorized as moderate to very strong. According to the one-way ANOVA statistical test, all combinations were significantly different (p &lt; 0.05). The ethanol extract of sweet orange peel contains flavonoids, alkaloids, phenolics and steroids, while the kepok banana peel extract contains phytochemical compounds such as flavonoids, phenolics, triterpenoids and alkaloids.

ANALYSIS OF THE INFLUENCE OF AGE ON THE ABSORBED DOSE OF X-RAY RADIATION IN THORAX EXAMINATION

Analysis of the Effect of Age on the Absorption Dose of X-ray Radiation in Thorax Examination. This research aimed at finding out the effect of the patient's age on the absorbed dose obtained during the thorax examination. The observation data used is the exposure factor in the form of the tube voltage (kV), tube current (mA), irradiation time (s), and focus film distance (cm). The age range used is grouped based on the elderly age category established by the Ministry of Health of the Republic of Indonesia which ranges from 46 years until 85 years of age. From the data on the exposure factor and age of elderly patients, the oneway ANOVA statistical test was conducted. From the results of these statistical tests, the results obtained FCalculate value is 0.201 and sig 0.821 for AP thorax examination and the results obtained FCalculate value is 0.790 and sig 0.476 for PA thorax examination with the FTable value is 3.81 and the sig is 0.05. Therefore, based on the testing criteria in statistics of the initial hypothesis (H0) is accepted or it can be concluded that age does not affect the amount of X-ray radiation absorbed dose received by elderly patients in thorax examinations from the age range of 46 years until 85 years.

The Effect of Ciplukan Leaves Extract (Physalis minima) Effervescent Tablets as an Denture Cleanser to the Growth Candida albicans

Denture stomatitis is one of the problems that often occurs in denture wearers. Prevention of denture stomatitis can be done by immersing the denture in a cleaning solution. Ciplukan leaves (Physalis minima) are natural ingredients that can be used for denture cleaning because they contain antimicrobials. This study aims to determine the effect of effervescent tablets of ciplukan leaf extract (Physalis minima) as a denture cleanser in inhibiting the growth of C. albicans. Methods: This type of research is a laboratory experiment with the post-test-only control group design. The sample used is acrylic resin plate made of heat curing acrylic material The samples used were 24 pieces with a size of 10x10x1 mm which were divided into 4 treatment groups. Samples were immersed in alkaline peroxide effervescent tablets and ciplukan leaf extract effervescent tablets with concentrations of 25%, 50%, and 75% for 20 minutes. The number of C. albicans colonies was counted using a spectrophotometer and converted into the formula. The results of the One-Way ANOVA statistical test showed that the number of C. albicans in each treatment group was significantly different with a significance value of 0,00. Ciplukan leaf extract effervescent tablets were able to inhibit the growth of C. albicans on heat cured acrylic resin plates with the most effective concentration of 75%.

IDENTIFICATION AND DETERMINATION OF SAPONIN CONTENT FROM EXTRACT OF Embelia borneensis BARK

Abstract Embelia borneensis known sekilang used by the Dayak Kenyah tribe of Long Temuyat Village, Malinau, North Kalimantan as a fish poison and leech repellent. Sekilang bark extract contains alkaloids, tannins, saponins and flavonoids compounds. The purpose of this study was to determine the type and content of saponins in the methanol extract based on different maceration, reflux, and Soxhlet extraction methods. Saponin content was determined by the gravimetric method. The results showed that the type of saponin contained in sekilang bark extract was triterpenoid saponin. The average saponin content with maceration method was 24.87%, reflux method was 28.97 and soxhlation method was 29.99%. The results of data analysis with one-way anova statistical test showed that there was a significant difference between extraction methods on saponin content of sekilang stem bark with sig value of 0.009 with a significant level of 0.05 (5%).

HISTOLOGICAL RESPONSE OF PULP TO BELIMBING WULUH (Averrhoa bilimbi L.) GEL COMPARED TO CARBAMID PEROXIDE AS TOOTH WHITENING (IN VIVO)

A teeth whitening procedure is an action to get naturally bright teeth color. The teeth whitening process often has side effects on the tooth structure and its surroundings. Teeth whitening ingredients generally contain carbamide peroxide. One of the natural ingredients that can whiten teeth is Averrhoa bilimbi Linn. This study aimed to determine the effect of Averrhoa bilimbi L. gel and carbamide peroxide on the histological response of the pulp. The research method was carried out by processing Averrhoa bilimbi Linn into extracts through the maceration method and then making a gel with a concentration of 50%. The study was conducted on 27 rabbit incisors and divided into three groups (negative control group, 10% carbamide peroxide, and 50% Averrhoa bilimbi L. gel). Each group was exposed for 4 hours for 14 days, then the teeth were extracted, and the pulp was taken with K-file number 10-40 and continued to look at the histological picture. The data was processed using a one-way ANOVA statistical test; the statistical test results showed significant differences for the three groups with a p-value of 0.000 (p &lt;0.05). It can be concluded that there is an effect of 50% Averrhoa bilimbi L. gel on the histological response of the pulp, there is an effect of 10% carbamide peroxide on the histological response of the pulp, and there is a significant difference between 10% carbamide peroxide and 50% Averrhoa bilimbi L. gel.

Multi-objective optimization of mixed-model assembly lines incorporating musculoskeletal risks assessment using digital human modeling

In line with Industry 5.0, ergonomic factors have recently received more attention in balancing assembly lines to enhance the human-centric aspect. Meanwhile, today’s mass-customized trend yields manufacturers to offset the assembly lines for different product variants. Thus, this study addresses the mixed-model assembly line balancing problem (MMALBP) by considering worker posture. Digital human modeling and posture assessment technologies are utilized to assess the risks of work-related musculoskeletal disorders using a method known as rapid entire body analysis (REBA). The resulting MMALBP is formulated as a mixed-integer linear programming (MILP) model while considering three objectives: cycle time, maximum ergonomic risk of workstations, and total ergonomic risks. An enhanced non-dominated sorting genetic algorithm (E-NSGA-II) is developed by incorporating a local search procedure that generates neighborhood solutions and a multi-criteria decision-making mechanism that ensures the selection of promising solutions. The E-NSGA-II is benchmarked against Epsilon-constraint, MOGA, and NSGA-II while solving a case study and also test problems taken from the literature. The computational results show that E-NSGA-II can find promising Pareto front solutions while dominating the considered methods in terms of performance metrics. The robustness of E-NSGA-II results is evaluated through one-way ANOVA statistical tests. The analysis of results shows that a smooth distribution of time and ergonomic loads among the workstations can be achieved when all three objectives are simultaneously considered.