One-way ANOVA Statistical Test Research Articles

Antibacterial activity of jamblang leaf ethanol extract (Syzygium cumini) against the growth of Propionibacterium acnes

Background: Propionibacterium acnes is a bacterium found on the skin that plays a significant role in acne vulgaris. The inappropriate use of antibiotics can lead to resistance, necessitating the search for alternative therapies from plants with high antibacterial potential. One such plant is jamblang (Syzygium cumini), which has demonstrated antibacterial properties. Objective: This research aims to test the antibacterial activity of jamblang leaf extract against the growth of P. acnes. Method: The study employed an experimental design with a posttest-only control group design. The treatment groups consisted of a positive control (clindamycin), a negative control (sterile distilled water), and jamblang leaf extract groups with concentrations of 100%, 50%, 25%, 12.5%, and 6.25% (n=4). Data analysis was conducted using the One-Way ANOVA statistical test. Results: The jamblang leaf extract exhibited antibacterial potential against the growth of P. acnes. The extract showed strong antibacterial activity at concentrations of 100%, 50%, 25%, and 12.5%, and moderate antibacterial activity at a concentration of 6.25%. Conclusion: This suggests that jamblang leaf extract could be a promising alternative therapy for treating acne vulgaris, offering a natural solution to combat antibiotic resistance.

Formulation and Characterization of New Experimental Dental Composites with Zirconium Filling in Different Forms.

Short glass fibers are generally used in posterior dental restorations to enhance the mechanical properties and improve the material microstructure. Two resin-based composites (S0 and SF) were formulated and characterized to investigate the influence of zirconium in their characteristics and properties. The organic part of the investigated materials was the same (BisGMA, TEGDMA, and a photochemical polymerization system), and in the inorganic part, besides quart, glassA, and hydroxylapatite with Zn, sample S0 contained strontium glass with zirconium and sample SF contained fiber powder of chopped zirconium. The samples were characterized by the degree of conversion (DC), mechanical properties, water sorption (WS), scanning electron microscopy (SEM), atomic force microscopy (AFM) before and after the WS test, and antimicrobial properties. The results obtained were subjected to one-way ANOVA and Tukey's statistical tests. Both samples had a high DC. Regarding the mechanical properties, both samples were very similar, except DTS, which was higher for the composite without fibers. After 14 days, the WS value of the SF sample was lower than that of the S0 sample. Water caused significant changes in the topography of the SF sample, but thanks to its antimicrobial properties and the diffusion phenomenon, SF had a more pronounced antimicrobial effect. This study shows that the addition of appropriate amounts of Sr-Zr-glass powder gives the material in which it is added similar properties to material containing chopped zirconium glass fiber powder. According to the antimicrobial test results, resin composites containing experimental zirconia fillings can be considered in future in vitro clinical studies for posterior reconstructions with significantly improved mechanical properties.

The Impact of Butterfly Pea Flower (Clitoria ternatea L.) Extract on Atherosclerosis Biomarker Profiles in Obese White Rats (Rattus norvegicus L.)

Background: Clitoria ternatea L., commonly known as butterfly pea flower, has gained attention as a potential agent in the prevention and treatment of atherosclerosis due to its rich content of bioactive compounds, such as anthocyanin, a type of flavonoid renowned for its potent antioxidant and anti-inflammatory properties, along with its ability to enhance the immune system.Objective: This study aimed to elucidate the positive impact of butterfly pea flower extract on atherosclerosis biomarker profiles.Methods: The approach framework used in this research is a true experimental laboratory with a Control Group Post-Test design. Obese male white rats were selected as the research subjects. Thirty-six obese white rats were randomly divided into six groups using the Completely Randomized Design (CRD) method. The data collected were VCAM and IL-6 levels from the blood serum of obese white rats tested using an ELISA photometer. The tunica intima thickness was measured using a microscope. Data were analyzed employing SPSS 18 software, utilizing one-way ANOVA statistical tests and post hoc Tukey tests.Results: The research showed that with the increase in Butterfly Flower Extract (BPFE) dose, there was a consistently lower level of inflammatory biomarkers, such as VCAM-1 and IL-6, compared to positive controls and other variations, as well as tunica intima thickness was thinning than others. A dose of 600 mg/kg BW (P3 group) had VCAM-1 levels up to 30.40 ± 6.71 ng/mL, IL-6 levels up to 17.70 ± 8.29 ng/mL, and tunica intima thickness up to 3.18 ± 1.24 µm.Conclusion: BPFE effectively lowers inflammatory biomarkers and thins the tunica intima thickness in obese white rats at 600 mg/kg BW dose. It may offer promising therapeutic potential in addressing atherosclerosis.

Potential Pharmaceutical Biotechnology Products in the Form of Formulations and Preparations of Telang Flower Kombucha Dish Soap (Clitoria ternatea L) as an Antibacterial from Beef (Bos taurus) Isolate

This research aims to see the potential of each formulation and preparation of dishwashing soap with the active ingredient telang flower kombucha in inhibiting the growth of pathogenic bacteria isolated from beef. This research is a laboratory experiment, namely by making a dishwashing soap base without the active substance of telang flower kombucha. Make a dishwashing soap base that adds the active substance of butterfly pea flower kombucha at concentrations of 20%, 30% and 40%. Biochemical identification results have shown that beef samples contain gram-positive bacteria in the form of Staphylococcus aureus and Listeria monocytogenes, as well as gram-negative bacteria in the form of Salmonella thyposa and Escherichia coli. The results of this research have also proven that pharmaceutical biotechnology products in the form of formulations and dishwashing soap preparations with the active ingredient telang flower kombucha have a positive correlation in inhibiting the growth of the four test bacteria. Based on the one-way ANOVA statistical test at each P value <0.05, it proves that telang flower kombucha dish soap at a concentration of 40% according to post hoc analysis is not significantly different from concentrations of 20% and 30% as a pharmaceutical and antibacterial biotechnology product. isolated from beef. The conclusion of this research is that the formulation and preparation of dishwashing soap at a concentration of 40% proved to be the most effective as a pharmaceutical biotechnology product in inhibiting the growth of the four test bacteria.

Total Flavonoid Content and In Vitro Anti-Inflammatory Potentials of Kombucha with Enrichment of Butterfly Pea (Clitoria ternatea) Flower Extract

Kombucha is a fermented beverage from tea (Camellia sinensis) that is claimed to be beneficial for several medical ailments. Several studies have conducted nutritional enrichment of kombucha to optimize its nutritional benefits. One of the native Indonesian plants that can be utilized as nutritional enrichment material is the butterfly pea (Clitoria ternatea) flower. Its nutrition is expected to increase the health benefits of kombucha Camellia sinensis. Kombucha has beneficial compounds such as flavonoids that have potential applications in medical chemistry due to its anti-inflammatory properties. This study aims to enrich the valuable nutrition and test the in vitro anti-inflammatory potentials of kombucha, conducted by formulating kombuchaCamellia sinensis with Clitoria ternatea flower extract addition (0; 0.5; 1; 1.5; 2)% (m/v), the chemical characterization including pH value test using digital pH meter and total flavonoid content (TFC) using colorimetric (AlCl3) assay) with quercetin as its standard, and in vitro anti-inflammatory activity test using the red blood cell (RBC) membrane stabilization method. The result showed that the pH value of kombucha had met the consumption quality standard (3-4), starting from 3,30 + 0,00 (0%) to 3,66 + 0,01 (2%). The flavonoid content increased from 38,89 + 0,05 mgQE/g (0%) to 126,73 + 0,13 mgQE/g (2%). Kombucha samples showed anti-inflammatory activity potentials with the best IC50 value of 140,22 ppm (2% addition). The data from this study were analyzed using a one-way ANOVA statistical test. It shows there is an influence from the addition of Clitoria ternatea to the TFC result (p<0,05), and it continued to Duncan's Multiple Range test (DMRT) that showed sample F0 to F4 were significantly different from each other (p<0,05). In conclusion, the enrichment of kombucha (Camellia sinensis) using Clitoria ternatea flower extract has been proven to increase the health-beneficial compound (flavonoids) and have potential anti-inflammatory activity.

First Report of Binucleate Rhizoctonia AG-G Causing Grapevine (Vitis Vinifera) Trunk Diseases in California Nurseries.

Grapevine Trunk Diseases (GTD) are caused by a consortium of fungal pathogens that affect the biological functions of the vascular system of mature and young grapevines (Gramaje et al. 2018). We conducted surveys to profile GTD pathogens in California grapevine nurseries and collected 784 cuttings of cvs. Cabernet Sauvignon and Chardonnay grafted on 1103P rootstock. Several vines exhibited wood necrotic lesions and cankers at the graft union and the root ball (Figure 1A). Symptomatic wood tissues were cultured on PDA medium and after two weeks of incubation at room temperature (22°C), several known GTD pathogens were recovered. We also identified Rhizoctonia from 42 of the 784 vines (5.3% incidence) based on the morphological characteristics of a brown pigmented mycelium (Figure 1B), hyphae branched at a right angle with constrictions at the branch point (Figure 1C) and absence of spores (González García et al., 2006). A subsample of four isolates (DCHG2B, DCSG22R, JCSG9B, and JCHG12B) were randomly selected for further DNA-based taxonomic identification and pathogenicity evaluation to grapevine. The ITS and beta tubulin regions were amplified using the ITS1/ITS4 and B36F/B12R primer sets, respectively (González et al. 2006), and sequences were deposited in the NCBI database (Accession numbers: OR052655, OR052656, OR052657, OR052658 and OR059207, OR059208, OR059209, OR059210). Sequences displayed >99% and >96% identity with the respective ITS and beta tubulin sequences of the binucleate Rhizoctonia AG-G specimen C-653 (González et al. 2006). A phylogenetic tree constructed using the Neighbor-Joining method indicated a 100% bootstrap support with the binucleate Rhizoctonia AG-G (Figure 2). Pathogenicity of the binucleate AG-G Rhizoctonia were confirmed on two separate technical replicates using standard methods. For each replicate, one-year-old rootstock 1103P were wounded with sterile drill bits and inoculated with a single 5 mm diameter agar plug collected from Rhizoctonia growing cultures, while control vines were inoculated with sterile agar. The first replicate lasted 28 weeks with (DCHG2B, DCSG22R) inoculated on seven vines. The second bioassay lasted 24 weeks with two additional isolates (JCSG9B, JCHG12B) inoculated on twelve vines. Rhizoctonia-inoculated vines developed wood symptoms similar to those observed on cuttings in nurseries, with necrotic lesions lengths significantly longer than the controls (First replicate: 3.5  0.4 cm vs. 1.3  0.6 cm; Second replicate: 6.8  0.8 cm vs. 1.1  0.2 cm), based on one-way ANOVA statistical test (P value < 0.05). Rhizoctonia isolates recovery from wood necrotic lesions were confirmed by ITS sequencing, thereby fulfilling Koch's postulate. Several binucleate Rhizoctonia anastomosis groups, including AG-G, have been found to cause root rot and stem necrosis in plant nurseries (Aiello et al., 2017; Rinehart et al., 2007). Rhizoctonia has also been reported to be associated with grapevine nurseries in Europe (Pintos et al., 2018), South Africa (Halleen et al., 2003) and Australia (Walker, 1992). However, the multinucleate Rhizoctonia solani was the only species confirmed to cause root rot on grapevine (Walker, 1992). Our data suggests that the binucleate Rhizoctonia from the AG-G anastomosis group also cause wood necrosis in grapevine. Those findings warrant further studies on the complexity of Rhizoctonia anastomosis groups in nursery and their aggressiveness to grapevine.

Uji Aktivitas Antifungi Kombinasi Ekstrak Etanol Kulit Pisang Kepok (Musa paradisiaca L.) dan Kulit Jeruk Manis (Citrus sinensis) terhadap Candida albicans Penyebab Keputihan Patologis Secara In Vitro

Pathological vaginal discharge (Candidiasis) is a reproductive health problem experienced by more than 50% of women throughout the world. Candida albicans is the main cause of pathological vaginal discharge. This disease is characterized by yellow to greenish discharge, odor, and itching to the point of bleeding which can cause death. Antifungal drugs used to treat pathological vaginal discharge can have several side effects. Therefore, the urgency of this research is to find natural alternatives for pathological vaginal discharge drugs, such as sweet orange peel and kepok banana peel which contain bioactive compounds that can inhibit the growth of Candida albicans. The aim of this study was to test the antifungal activity of a combination of ethanol extracts of sweet orange peel and kepok banana peel against Candida albicans in vitro. This research uses laboratory experimental methods. The results showed that all combinations of ethanol extracts of sweet orange peel and kepok banana peel could inhibit the growth of Candida albicans. The inhibitory power of each extract combination is categorized as moderate to very strong. According to the one-way ANOVA statistical test, all combinations were significantly different (p &lt; 0.05). The ethanol extract of sweet orange peel contains flavonoids, alkaloids, phenolics and steroids, while the kepok banana peel extract contains phytochemical compounds such as flavonoids, phenolics, triterpenoids and alkaloids.

ANALYSIS OF THE INFLUENCE OF AGE ON THE ABSORBED DOSE OF X-RAY RADIATION IN THORAX EXAMINATION

Analysis of the Effect of Age on the Absorption Dose of X-ray Radiation in Thorax Examination. This research aimed at finding out the effect of the patient's age on the absorbed dose obtained during the thorax examination. The observation data used is the exposure factor in the form of the tube voltage (kV), tube current (mA), irradiation time (s), and focus film distance (cm). The age range used is grouped based on the elderly age category established by the Ministry of Health of the Republic of Indonesia which ranges from 46 years until 85 years of age. From the data on the exposure factor and age of elderly patients, the oneway ANOVA statistical test was conducted. From the results of these statistical tests, the results obtained FCalculate value is 0.201 and sig 0.821 for AP thorax examination and the results obtained FCalculate value is 0.790 and sig 0.476 for PA thorax examination with the FTable value is 3.81 and the sig is 0.05. Therefore, based on the testing criteria in statistics of the initial hypothesis (H0) is accepted or it can be concluded that age does not affect the amount of X-ray radiation absorbed dose received by elderly patients in thorax examinations from the age range of 46 years until 85 years.

The Effect of Ciplukan Leaves Extract (Physalis minima) Effervescent Tablets as an Denture Cleanser to the Growth Candida albicans

Denture stomatitis is one of the problems that often occurs in denture wearers. Prevention of denture stomatitis can be done by immersing the denture in a cleaning solution. Ciplukan leaves (Physalis minima) are natural ingredients that can be used for denture cleaning because they contain antimicrobials. This study aims to determine the effect of effervescent tablets of ciplukan leaf extract (Physalis minima) as a denture cleanser in inhibiting the growth of C. albicans. Methods: This type of research is a laboratory experiment with the post-test-only control group design. The sample used is acrylic resin plate made of heat curing acrylic material The samples used were 24 pieces with a size of 10x10x1 mm which were divided into 4 treatment groups. Samples were immersed in alkaline peroxide effervescent tablets and ciplukan leaf extract effervescent tablets with concentrations of 25%, 50%, and 75% for 20 minutes. The number of C. albicans colonies was counted using a spectrophotometer and converted into the formula. The results of the One-Way ANOVA statistical test showed that the number of C. albicans in each treatment group was significantly different with a significance value of 0,00. Ciplukan leaf extract effervescent tablets were able to inhibit the growth of C. albicans on heat cured acrylic resin plates with the most effective concentration of 75%.

IDENTIFICATION AND DETERMINATION OF SAPONIN CONTENT FROM EXTRACT OF Embelia borneensis BARK

Abstract Embelia borneensis known sekilang used by the Dayak Kenyah tribe of Long Temuyat Village, Malinau, North Kalimantan as a fish poison and leech repellent. Sekilang bark extract contains alkaloids, tannins, saponins and flavonoids compounds. The purpose of this study was to determine the type and content of saponins in the methanol extract based on different maceration, reflux, and Soxhlet extraction methods. Saponin content was determined by the gravimetric method. The results showed that the type of saponin contained in sekilang bark extract was triterpenoid saponin. The average saponin content with maceration method was 24.87%, reflux method was 28.97 and soxhlation method was 29.99%. The results of data analysis with one-way anova statistical test showed that there was a significant difference between extraction methods on saponin content of sekilang stem bark with sig value of 0.009 with a significant level of 0.05 (5%).

HISTOLOGICAL RESPONSE OF PULP TO BELIMBING WULUH (Averrhoa bilimbi L.) GEL COMPARED TO CARBAMID PEROXIDE AS TOOTH WHITENING (IN VIVO)

A teeth whitening procedure is an action to get naturally bright teeth color. The teeth whitening process often has side effects on the tooth structure and its surroundings. Teeth whitening ingredients generally contain carbamide peroxide. One of the natural ingredients that can whiten teeth is Averrhoa bilimbi Linn. This study aimed to determine the effect of Averrhoa bilimbi L. gel and carbamide peroxide on the histological response of the pulp. The research method was carried out by processing Averrhoa bilimbi Linn into extracts through the maceration method and then making a gel with a concentration of 50%. The study was conducted on 27 rabbit incisors and divided into three groups (negative control group, 10% carbamide peroxide, and 50% Averrhoa bilimbi L. gel). Each group was exposed for 4 hours for 14 days, then the teeth were extracted, and the pulp was taken with K-file number 10-40 and continued to look at the histological picture. The data was processed using a one-way ANOVA statistical test; the statistical test results showed significant differences for the three groups with a p-value of 0.000 (p &lt;0.05). It can be concluded that there is an effect of 50% Averrhoa bilimbi L. gel on the histological response of the pulp, there is an effect of 10% carbamide peroxide on the histological response of the pulp, and there is a significant difference between 10% carbamide peroxide and 50% Averrhoa bilimbi L. gel.

Multi-objective optimization of mixed-model assembly lines incorporating musculoskeletal risks assessment using digital human modeling

In line with Industry 5.0, ergonomic factors have recently received more attention in balancing assembly lines to enhance the human-centric aspect. Meanwhile, today’s mass-customized trend yields manufacturers to offset the assembly lines for different product variants. Thus, this study addresses the mixed-model assembly line balancing problem (MMALBP) by considering worker posture. Digital human modeling and posture assessment technologies are utilized to assess the risks of work-related musculoskeletal disorders using a method known as rapid entire body analysis (REBA). The resulting MMALBP is formulated as a mixed-integer linear programming (MILP) model while considering three objectives: cycle time, maximum ergonomic risk of workstations, and total ergonomic risks. An enhanced non-dominated sorting genetic algorithm (E-NSGA-II) is developed by incorporating a local search procedure that generates neighborhood solutions and a multi-criteria decision-making mechanism that ensures the selection of promising solutions. The E-NSGA-II is benchmarked against Epsilon-constraint, MOGA, and NSGA-II while solving a case study and also test problems taken from the literature. The computational results show that E-NSGA-II can find promising Pareto front solutions while dominating the considered methods in terms of performance metrics. The robustness of E-NSGA-II results is evaluated through one-way ANOVA statistical tests. The analysis of results shows that a smooth distribution of time and ergonomic loads among the workstations can be achieved when all three objectives are simultaneously considered.

Analysis of the Effect of Age on the Absorption Dose of X-ray Radiation in Thorax Examination

This research aimed at finding out the effect of the patient's age on the absorbed dose obtained during the thorax examination. The observation data used is the exposure factor in the form of the tube voltage (kV), tube current (mA), irradiation time (s), and focus film distance (cm). The age range used is grouped based on the elderly age category established by the Ministry of Health of the Republic of Indonesia which ranges from 46 years until 85 years of age. From the data on the exposure factor and age of elderly patients, the oneway ANOVA statistical test was conducted. From the results of these statistical tests, the results obtained FCalculate value is 0,201 and sig 0,821 for Thorax AP examination and the results obtained FCalculate value is 0,790 and sig 0,476 for Thorax PA examination with the FTable value is 3,81 and the sig is 0,05. Therefore, based on the testing criteria in statistics of the initial hypothesis (H0) is accepted or it can be concluded that age does not affect the amount of X-ray radiation absorbed dose received by elderly patients in thorax examinations from the age range of 46 years until 85 years.

Utilization Trends of the Kisan Credit Card (KCC) Beneficiaries

One of the most essential resources in contemporary agriculture is credit. As a result, there is a need to expand agricultural finance, boost land productivity, and raise the potential and effectiveness of using water resources for agricultural output. Credit is needed, among other things, for the adoption of agricultural technology, the purchase of contemporary inputs and tools, the development of land, the purchase of animals, and the purchase of raw materials. By enabling farmers to satisfy their credit needs throughout the whole cycle of crop production and at the same time providing money for investment reasons, agriculture credit plays a significant role in preserving agricultural production. To better understand "Utilization Trends of Kisan Credit Card (KCC) Beneficiaries," the current study was proposed. Descriptive research design was used for the study. For this study, 120 farmers were specifically chosen as a sample from the Eligaid mandal of Peddapalli District of Telangana Sate. According to the data, the majority of respondents had socio-economic profiles that are on the middle level. It was discovered that majority of respondents had a medium level of utility for the Kisan Credit Card. Age, education, home type; landholding, annual income, family type, savings increased, income levels increased by this scheme and level of utility etc. were all found to be significantly associated with the credit utilization of the Kisan credit card at 0.05 level of significance by applied the chi-square and one-way ANOVA statistical tests in SPSS.

Separate and Combined Effects of Resistance Training and Cucumber (Cucumis sativus) Juice Consumption on the Diabetic Indicators and Lipid Profile in Women with Type 2 Diabetes

Background: Type 2 diabetes is a metabolic disease with many consequences. Objectives: This study aimed to evaluate the separate and combined effects of resistance training and cucumber (Cucumis sativus) juice consumption on diabetic indicators and lipid profiles in women with type 2 diabetes. Methods: Women with type 2 diabetes (n = 40) were assessed in a randomized, double-blind study and categorized into four groups: (training + placebo), (training + supplement), supplement, and control. Blood sampling was performed 48 hours before and after the protocol, and blood indicators were evaluated to compare their changes among groups. One-way ANOVA statistical tests and Tukey’s post hoc test were used to compare groups. Results: The results indicated that separate resistance training significantly decreased fasting blood sugar (FBS), glycated hemoglobin (HbA1c), triglyceride (TG), total cholesterol (TC), and low-density lipoprotein (LDL), while high-density lipoprotein (HDL) significantly increased. Cucumber juice consumption significantly reduced FBS, HbA1c, TG, TC, and LDL, while HDL significantly increased (P &lt; 0.05). Combined resistance training and consuming cucumber juice caused a more significant decrease in (FBS, HbA1c, TG, TC, and LDL), and HDL increased significantly (P &lt; 0.05). Intergroup changes in (FBS, TG, TC, LDL) was significant (P &lt; 0.05). Intergroup changes in (HbA1c and HDL) were significant (P &lt; 0.05). However, differences in (HbA1c and HDL) between training and supplement groups were not significant (P ≥ 0.05). Conclusions: Separate and combined effects of resistance training and cucumber juice consumption improved diabetic indicators and lipid profile in women with type 2 diabetes.

U UJI AKTIVITAS ANTIBAKTERI SEDIAAN GEL HAND SANITIZER SANITIZER BUNGA LAWANG (Illiacium verum Hook. f) pada BAKTERI Staphylococcus aureus &amp; Escherichia coli

Some diseases caused by microorganisms on the hands are diarrhea, diphteria, boils, typhus and others. The bacteria that most often contaminate the skin of the hands are Staphylococcus aureus and Escheria coli, whose spreas most often transmitted from hand to hand. Bunga lawing are one of the medicinal plants that have been used in medicine. The purpose of this study was determine the antibacterial activity of gel hand essential oil sanitizer gel preparation (Illiacium verum Hook.f) to the growth of Staphylococcus aureus and Escheria coli bacteria compared to sanitizer Dettol gel hand. This was carried out with the experimental method. Essential oil of bunga lawing is extracted using a heat method, namely soxhletation. The antibacterial activity test in this study used the agar diffusion method (disc diffusion). The measurement of the diameter of the inhibited area formed were analyzed using a one-way ANOVA statistical test. The results of antibacterial activity of bunga lawang sanitizer hand have lower antibacterial activity gel hand sanitizer Dettol. In the diameter of Staphylococcus aureus bacteria the highest rate 0f hand flower sanitizer gel was 15,1 mm while the sanitizer gel hand wash 21,4 mm. In Escheria coli bacteria, the inhibitory diameter the sanitizer hand gel dettol was obtained at 20.1 mm, while the lawang hand sanitizer gel obtained the highest inhibition diameter of 13.1 mm. The results of data analysis using a one-way ANOVA statistical test showed a sig value p&lt;0,05 so that there were significant differences between the gel hand to the inhibitory power of the growth of Staphylococcus aureus and Escheria coli bacteria.

Uji komparasi desinfektan septalkan, terralin, dan alkohol 70% terhadap daya sterilisasi permukaan kursi dental

Cross-infection has the potential to occur in the Dental and Oral Hospital due to cross-transmission of pathogenic microbes through dental chair surfaces that are contaminated with microbes. This study aims to compare the effectiveness of three types of disinfectant Septalcan, Terralin, and 70% alcohol on the inhibition of pathogenic microbes originating from the oral cavity of patients who contaminate the surface of dental chairs in aerosol and non-aerosol clinics. Sampling of microorganisms was carried out using the technique of wiping/swab the surface of dental chairs in aerosol and non-aerosol clinics before and after the disinfection process with three types of disinfectants. The decrease in the number of germs before and after the disinfectant process is used as a parameter for the effectiveness of the disinfectant. The results of the one-way ANOVA statistical test shows that there is no significant difference in the effectiveness of the three types of Septalcan, Terralin, and 70% alcohol in reducing the number of germs on the surface of dental chairs. The three types of disinfectants are able to minimize the risk of cross-infection due to pathogenic microbial contamination on the surface of the dental chair.

The Influence of Oral Administration of Red Dragon Fruit Peels Extract (Hylocereus polyrhizus) on Increasing High Density Lipoprotein in Male White Rats (Rattus norvegicus) Hypercholesterolemia

Background: Hypercholesterolemia is one of the most common diseases in the world, with cholesterol levels exceeding normal levels. Hypercholesterolemia can cause various systemic disorders in the body. One of the signs is by decreasing HDL (High-Density Lipoprotein) cholesterol levels. Dragon fruit peel contains many ingredients, such as vitamin B3 (niacin), vitamin C, flavonoids, anthocyanins, and other minerals that can affect the increase in HDL production and reduce total cholesterol levels. Purpose: To know the effect of red dragon fruit peel extract (Hylocereus polyrhizus)on increasing levels of High-Density Lipoprotein (HDL) in male rats (Rattus norvegicus) hypercholesterolemia. Methods: Male rats were made hypercholesterolemic using a mixture of lard (3 ml) and egg yolk (1 g) for 14 days orally. 20 hypercholesterolemic male rats were further divided into 5 treatment groups and given dragon fruit peel extract therapy with different treatments, namely T0 negative control (CMC-Na 1% + aquadest), T1 positive control (Simvastatin 0.0013 mg/200 g BW), T2, T3 and T4 (each 100, 150, 200 mg/kg BW red dragon fruit peel extract) for 14 days orally. Blood serum was taken on day 36 and tested in the laboratory using a chemical analyzer machine; then the data were analyzed using the univariant multiple way one-way ANOVA statistical test with a significant difference (p&gt;0.05). Results: The P4 treatment group has an effective dose (200 mg/kg BW) to increase high-density lipoprotein (HDL) levels, while the P3 treatment group (dose 150 mg/kg BW) is less effective but can still have an effect. Conclusion: This research showed that red dragon fruit peel extract could increase HDL (High-Density Lipoprotein) cholesterol levels with an effective 200 mg/kg BW dose.

Formulation of Reed Diffuser is A Combination of Cinnamon (Cinnamomomum Verum) and Citronella (Cymbopogon Nardus) Essential Oil as An Anti-Stress Aromatheraphy

This study aims to determine whether the combination formulation of essential oils between cinnamon and citronella can be used as an aromatherapy reed diffuser preparation that has acted as anti-stress aromatherapy. The method used in this research is an experimental laboratory with a post-test only control group design using mice as test animals. The population used in this study was Cinnamon (Cinnamomum verum) and citronella (Cymbopogon nardus), while the samples used were Cinnamon (Cinnamomum verum) and citronella (Cymbopogon nardus) originating from Antang Market, Makassar City, South Sulawesi. taken in the morning. Data collection was carried out using questionnaires and observation methods. A one-way ANOVA statistical test was used to analyze the obtained data. The results showed that the stability of the Reed Diffuser combination of Cinnamon (Cinnamomum verum) and citronella (Cymbopogon nardus) essential oils in organoleptic testing had a physically and chemically stable odor and color. The hedonic test of the odor produced shows a distinctive odor in all four formulas, but the most preferred is at a concentration of 2:3. In addition, the preparation of Reed Diffuser, a combination of Cinnamon (Cinnamomum verum) and citronella (Cymbopogon nardus) essential oils, is effective as an anti-stress based on the results of tests with regular use.

Effect of Soaking Time’s Variation of Red Onion (Allium Cepa L) Extracts on Formalin Content in Mackerel Tuna (Euthynnus Affinis)

Mackerel tuna (Euthynnus affinis) is one of the most popular foods in the community since it is affordable and has complete nutritional content. It is a frozen fish food product so the storage period of which is relatively short. In addition, it is possible to add formalin on which. Formalin is a substance that is prohibited from being used due to its dangerous effects. The content of saponin compounds found in red onions (Allium cepa L) can be used to reduce formalin levels. This study aimed to determine the effect of variations in the soaking time of red onion extract on the formalin level in mackerel tuna and the optimal soaking time for reducing formalin levels. This study used an experimental method with a posttest control group design. The treatment was given by soaking red onion extract at a concentration of 10% in mackerel tuna containing formalin with variations in soaking time of 1 hour, 2 hours, and 3 hours which were tested quantitatively using a UV-Vis spectrophotometer. Then, the data were analyzed using a one-way ANOVA statistical parametric test and a post hoc test using Duncan's test (DMRT). The decrease of the formalin levels by soaking the onion extract for 1 hour, 2 hours, and 3 hours was 10.61%, 41%, and 73%, respectively. The result showed that there was an effect of the soaking time of red onion extract on formalin levels (p-value 0.05), and the optimal time of soaking onion extract to reduce the formalin content of the samples was 3 hours.