Fatigue life of equipment cabin structure of CRH2G type train-set was taken into study in this paper. Aerodynamic load test points using differential pressure measurement were arranged at typical positions of equipment cabin structure, to obtain the aerodynamic load between inside and outside of equipment cabin. Adopting rain-flow counting method and finite element numerical simulation, 8-grade load spectrums of one-dimensional amplitude load and two-dimensional considering both amplitude and mean load were established. Fatigue life was then estimated based on Palmgren-Miner linear cumulative damage criterion. And the influence mechanism of operation conditions like train passing, tunnel passing on fatigue damage of equipment cabin structure was further analyzed. Results show that train passing conditions dominates in fatigue damage generation. The research results can provide scientific evidence and basis for the design, application and maintenance of high speed train.