Omega-3 Rich Oil Research Articles

Production of docosahexaenoic acid through enzymatic hydrolysis of Omega-3 rich oil

Docosahexaenoic acid (DHA), an essential omega-3 fatty acid, is crucial for the normal development and function of the brain. Its production can be achieved through the partial hydrolysis of DHA-rich triglycerides catalyzed by lipases, particularly from fish oils. In this work, the characterization of anchovy oil capsules revealed that they offer a pure and concentrated source of DHA, with more than 90 % of the oil in the form of triglycerides. Therefore, the hydrolysis reaction was studied with the aim of releasing 100 % of the DHA present in this oil, using different immobilized lipases with maximum enzyme loading on the hydrophobic support Immobeads-C18. The study’s results revealed that our immobilization strategy through hydrophobic adsorption improved the catalytic properties of activity and selectivity of the TLL lipase compared to other biocatalysts described in the literature. Additionally, complete hydrolysis of the oil was achieved in just 24 h with the NS40-C18 derivative, which could be reused up to 6 cycles without loss of activity.

Characterization and in vitro bioaccessability of optimized chia oil-Capsul-sodium alginate microparticles obtained by 3 nozzle spray-drying

The influence sodium alginate (SA) as an outer layer agent on bioaccessibility and matrix food release of purified chia oil (PCO) microencapsulated was investigated. PCO microparticles with Capsul were elaborated and optimized by response surface methodology (RSM), and then as an encapsulating agent and SA as an outer layer (PCO-Capsul/SA) were designed by mini spray-drying with 2 fluid nozzle (2-N) and 3 fluid nozzle (3-N). The optimal conditions obtained for PCO-Capsul system were: dryer inlet temperature of 114 °C and PCO:Capsul relation of 1:5.42. After the addition of SA out layer, PCO-Capsul/SA microparticles were subjected to in vitro static gastrointestinal digestion. PCO-Capsul/SA occurred mainly in the intestinal phase, showing the suitability of SA as an intestine-site release polymer. However, when PCO-Capsul/SA was incorporated into a yoghurt (Y), microparticles showed a significantly lower PCO matrix food release and bioaccessibility after in vitro digestion than PCO-Capsul-Y microparticles, due to their interaction between SA and Y. SA spray-dried by 3-N showed great potential for vehiculation of omega-3 rich oils in the future incorporation and develop of functional foods. Tweetable abstractThis research shows the role of the sodium alginate incorporation by 3 nozzle spray dryer when the design of an intestine-delivery food ingredient is intended.

Flaxseeds Composition And Phytochemical Screening Methods

Flaxseed, often known as linseed (Linum usitatissimum L.), is derived from the annual flax plant. The human nutrition industry is the primary application for flaxseed, as it is becoming recognized as a significant functional food component because to the active ingredient content, which is shown to have health advantages. Flaxseed can be consumed in a variety of forms, such as ground, as oil, or combined with baked goods. Flaxseed consumption is supported by scientific evidence due to its high content of omega-3 and omega-6 rich oil, lignans, α-linolenic acid, high-quality proteins, and fibers. These compounds are biologically active and can prevent various chronic diseases like diabetes, cardiovascular disease, cerebrovascular stroke, and many types of cancer. Additionally, benefits from flaxseed diet have been demonstrated in the field of animal nutrition, and as a result in healthier food from animal origin. In fact, the fatty acid profile of the meat and fat is directly affected by the source of fat in diet in swine and poultry, feeding omega-3 enriched diets by the addition of flaxseed would increase the omega-3 content in eggs and meat and thus enrich the products.

Assessment of omega-3 and omega-6 fatty acid profiles and ratio of omega-6/omega-3 of white eggs produced by laying hens fed diets enriched with omega-3 rich vegetable oil

Abstract The aim of this study was to evaluate the levels of omega-3 (n-3) and omega-6 (n-6) fatty acids in egg and the ratio of n-6/n-3 of white eggs produced by laying hens (Hy-Line white) fed diets enriched with n-3 fats. In this study, alpha-linolenic acid (ALA) levels of the dietary treatments ranged from 0.3 to 6% energy. Grain-based diets containing a low linoleic acid (LA) content were selected to prepare a basal diet to optimize the conversion of ALA into n-3 long chain polyunsaturated fatty acids (LCPUFA). The results showed that the level of all n-3 LCPUFA in eggs improved (P < 0.01) by increasing the levels of dietary ALA. Importantly, eggs produced from laying hens fed diets containing 6%en ALA significantly increased (P < 0.01) the total of n-3 fats by approximately nine-fold. Diets enriched with ALA significantly reduced (P < 0.01) the ratio of n-6/n-3 of the eggs. The n-6/n-3 ratio of eggs decreased from 7.17% in the 0.3%en ALA diet to 1.29% in the 6%en ALA diet. In conclusion, white laying hens fed ALA-enriched diets produced eggs higher in n-3 fatty acids and lower n-6/n-3 ratio, which provides an alternative n-3 rich food for consumers and have beneficial health effects.

Partial replacement of saturated fats in liver pâté by an olive oil-in-water emulsion containing β-glucan shows no compromise in sensory and storage oxidation of lipids and protein.

The consumption of olive oil has been shown to have a positive effect on preventing obesity and hypertension. At the same time, it is recommended to avoid processed meat products as they contain saturated fats. The inclusion of highly unsaturated lipids in food products can lead to rapid oxidation and deterioration of sensory characteristics. The objective of the current work was to encapsulate olive oil and incorporate it into traditional Polish liver pâté. The oil-in-water emulsions were formulated with varying levels of oat β-glucan and were evaluated for droplet size, pH, encapsulation efficiency and rheology. The liver pâtés made using the emulsions with and without β-glucan were then evaluated for pH, texture, colour, lipid and protein oxidation, thermal stability and sensory properties. The results showed that the oil-in-water emulsions had a 100% encapsulation rate of olive oil after 30 days of storage at 4 °C, regardless of the presence of β-glucan. Although the texture of the emulsion-enriched liver pâté was different from that of the control, this difference was reduced when β-glucan was added to the emulsion and then to the pâté matrix. Replacing 50% of animal fat with an olive oil emulsion enriched with β-glucan did not result in any compromise of sensory properties, increase lipid or protein oxidation. These results suggest that it is possible to replace saturated lipids with omega-3-rich olive oil. © 2024 Society of Chemical Industry.

High internal phase emulsions stabilized by pea protein isolate-inulin conjugates: Application as edible inks for 3D printing

High internal phase emulsions (HIPEs) are soft materials that can be designed to contain nutrients and active agents. As a result, they are being explored for their potential application as edible inks in 3D food printing applications. However, the rheological characteristics of HIPEs are often unsuitable for this purpose, resulting in unsatisfactory precision and resolution performance during 3D printing. This study aims to overcome these problems by creating HIPEs with improved rheological properties by using pea protein-inulin glycosylation products as plant-based emulsifiers. These conjugates were prepared from pea protein isolate (PPI) and inulin by glycosylation using a combined ultrasound/pH-shift method. The oil phase of these emulsions consists of omega-3 rich algal oil and lemon oil. We showed that stable HIPEs could be formed at an oil phase content of 80%. At this oil content, the oil droplets were uniform and stable, and the emulsions exhibited higher storage modulus. Compared to PPI alone or physical mixture of PPI and inulin, PPI-inulin conjugates formed oil droplets with smaller diameters and better uniformity, as well as thicker and denser biopolymer coatings. Compared with protein alone, the rheological analysis demonstrated that the HIPEs formulated with PPI-inulin conjugates had a higher viscosity, higher elasticity, higher recovery rate, and better thixotropy, indicating that they had physiochemical characteristics suitable for edible inks applications. This was confirmed by using the HIPEs as edible inks to 3D print model food objects. In conclusion, the pea protein-inulin conjugates fabricated in this study can be used as plant-based emulsifiers to form edible inks based on HIPEs. In principle, a range of nutrients and other bioactive agents could be incorporated into these inks for personalized nutrition applications.

The Effect of Omega-3 Rich Fish Oil on the Kidney Changes in Mice Induced by Azoxymethane and Dextran Sodium Sulfate

The Effect of Omega-3 Rich Fish Oil on the Kidney Changes in Mice Induced by Azoxymethane and Dextran Sodium Sulfate

A dose-response study with omega-3 rich canola oil as a novel source of docosahexaenoic acid (DHA) in feed for Atlantic salmon (Salmo salar) in seawater; effects on performance, tissue fatty acid composition, and fillet quality

Long-chain omega-3 rich oils from crops genetically modified with algal genes are a promising new source of the omega-3 fatty acid DHA (docosahexaenoic acid; 22:6n-3) in Atlantic salmon diets. In this study, the fish oil and fish meal content were held constant across diets, and conventional canola oil was replaced with DHA canola (DHA-CA) oil in a step-wise manner at 0%, 25%, 50%, and 100% replacement, respectively. Growth of salmon, reared in seawater, from approximately 465 g to 1.5 kg was equal across all dietary treatments. Survival was >98% in all cases, with no difference between the dietary groups. Digestibility of lipids was high, approximately 93% in all dietary groups. Fatty acid retention in whole body was also high, with greater retention of α-linolenic acid (ALA, 18:3n-3) and eicosapentaenoic acid (EPA; 20:5n-3) in groups containing higher proportions of DHA-CA oil. Fillet, liver, and intestinal fatty acid compositions were influenced by the different fatty acid contents of the diets, with progressively higher inclusion of ALA, EPA, and DHA and a higher n-3:n-6 ratio in fish fed the 25%, 50%, and 100% DHA-CA diets. Increased dietary inclusion levels of DHA reduced the conversion to 1-14C labelled ALA to 1-14C EPA in liver cells, but some conversion of ALA to 1-14C EPA was maintained even at high dietary DHA levels. There was also a significant increase in anti-inflammatory pro-resolving lipid mediators, resolvins, with increased dietary DHA-CA. The visual colour impression of both muscle and skin was improved at higher DHA-CA inclusion levels in diets.Overall, our findings demonstrates that DHA-CA oil is an effective ingredient in Atlantic salmon diets in seawater, and that fatty acids (FA) from this oil are incorporated into the fish tissues in a manner equivalent to FA from conventional sources, with positive effects on fillet quality.

Incorporation of hydroxytyrosol alkyl esters of different chain length as antioxidant strategy in walnut oil spray-dried microparticles with a sodium alginate outer layer

Omega-3 rich vegetable oils, such as walnut oil, are gaining interest because of their health benefits. Synthetized homologous series of hydroxytyrosol alkyl esters (HTEs) with different alkyl chain lengths (C4-C18) were incorporated in purified walnut oil (PWO) spray-dried microparticles, designed with Capsul® (C) as encapsulating agent and sodium alginate (SA) as outer layer (PWO-C/SA). The encapsulation efficiency (>87%) and Tg of PWO-C/SA microparticles were not affected by the HTEs. The incorporation of HTE-C10 increased the melting point (185.0 ± 1.3 °C), decreasing the formation of Dimers + Polymers (1.12 ± 0.05% at day 35 of storage) and the crystallinity of the microparticles (>170 °C). The highest stability of PWO-C(HTE-C10)/SA suggests a specific location of HTE-C10 at the oil:water interface. The SA layer delayed the release of fatty acids during in vitro digestion. The incorporation of HTEs of medium chain length can be a suitable strategy to protect unsaturated oils encapsulated by spray-drying.

Characterization and oxidation stability of spray-dried emulsions with omega-3 oil and buttermilk processed by ultra-high-pressure homogenization (UHPH).

Integrating functional ingredients, such as buttermilk and omega-3 rich oils, in spray-dried emulsions (SDE) is a suitable way to incorporate these ingredients in dairy products to substitute dairy fat and increase their added value. Ultra-high-pressure homogenization (UHPH) processing of liquid emulsions considerably improves stability compared to the conventional homogenization (CH) process. With this premise, SDE were produced while comparing CH (30 MPa) and UHPH (100 or 200 MPa) processing of feeding emulsions. Emulsions were formulated with (50:50 chia:sunflower) oil, whole commercial buttermilk (BM), and maltodextrin (MD) as wall materials. Further spray drying of emulsions was then conducted. Obtained SDE were characterized in terms of water content, Aw (water activity), flowing properties, water solubility, encapsulation efficiency (EE), color, and microstructure. Oxidation stability of SDE was analyzed in accelerated oxidation conditions at 50 °C for one month for primary and secondary oxidation analysis evolution on days 1, 7, 14, and 31 of storage. Results showed better ability of BM as encapsulating agent in UHPH-processed emulsions with 7% of BM. This improvement was especially observed in the flowing properties and encapsulating efficiency. Seven percent BM UHPH-treated SDE showed the best primary oxidation stability during storage, while the 4% BM-UHPH-treated SDE exhibited better secondary oxidative stability.

Sustainable Upcycling of Fisheries and Aquaculture Wastes Using Fish-Derived Cold-Adapted Proteases.

The fisheries and aquaculture industries are some of the major economic sectors in the world. However, these industries generate significant amounts of wastes that need to be properly managed to avoid serious health and environmental issues. Recent advances in marine waste valorization indicate that fish waste biomass represents an abundant source of high-value biomolecules including enzymes, functional proteins, bioactive peptides, and omega-3 rich oils. Enzyme-assisted processes, for the recovery of these value-added biomolecules, have gained interest over chemical-based processes due to their cost-effectiveness as well as their green and eco-friendly aspects. Currently, the majority of commercially available proteases that are used to recover value-added compounds from fisheries and aquaculture wastes are mesophilic and/or thermophilic that require significant energy input and can lead to unfavorable reactions (i.e., oxidation). Cold-adapted proteases extracted from cold-water fish species, on the other hand, are active at low temperatures but unstable at higher temperatures which makes them interesting from both environmental and economic points of view by upcycling fish waste as well as by offering substantial energy savings. This review provides a general overview of cold-adapted proteolytic enzymes from cold-water fish species and highlights the opportunities they offer in the valorization of fisheries and aquaculture wastes.

Stability assessment of emulsion of carotenoids extracted from carrot bio-waste in flaxseed oil and its application in food model system

The research was conducted on the stability assessment of emulsion of carotenoids extracted from carrot-pomace in omega-3 rich flaxseed oil, followed by converting this emulsion into freeze-dried powder, and its application as natural functional colorant in the preparation of flavored milk. The study indicated that emulsion was stable over a range of pH (5–9), temperature (forewarming to HTST pasteurization) and ionic strength (0.1–0.75 M NaCl) except at pH 4, sterilization temperature and 1.0 M NaCl based on zeta potential and particle size of emulsion analysed at these conditions. Ten percent (out of 8, 10 and 12%) of freeze-dried emulsion was selected for addition as a natural functional ingredient in flavored milk on the basis of organoleptic evaluation. The fat, protein, total solids, total carotenoid content and antioxidant value (in terms of ABTS and reducing power assay) of developed product were significantly higher (p < 0.05) than control. The whiteness index of control was significantly higher (p < 0.05) than the developed product, while reverse was true for yellowness index. However, both the products showed total color difference of 34.65. The research concludes that carotenoid-rich emulsion can be efficiently freeze-dried and used as a functional natural colorant in flavored milk for providing it better bio-functional attributes over control.

Lipid Indexes and Quality Evaluation of Omega-3 Rich Oil from the Waste of Japanese Spanish Mackerel Extracted by Supercritical CO2.

Japanese Spanish mackerel (JSM) (Scomberomorus niphonius) is a marine fish species containing health-beneficial polyunsaturated fatty acids (PUFAs). In the present study, the quality of JSM by-products oils extracted by supercritical CO2 (SC-CO2) and organic solvent extraction was compared in terms of physico-chemical properties of the oils. Eicosapentaenoic acid (EPA) is one of the important polyunsaturated fatty acids present in SC-CO2-extracted skin and muscle oil 5.81 ± 0.69% and 4.93 ± 0.06%, respectively. The amount of docosahexaenoic acid (DHA) in SC-CO2-extracted skin and muscle oil was 12.56 ± 0.38% and 15.01 ± 0.28%, respectively. EPA and DHA are considered as important PUFAs for the development of brain function and the prevention of coronary heart diseases. Extracted oils showed considerable antioxidant activity. In the obtained oils, atherogenic index (AI) values varied from 0.72 to 0.93 and thrombogenic index (TI) ranged from 0.75 to 0.92, which is considered an acceptable level. Fatty acid composition, bio potentiality, thermogravimetric, and vitamin D analysis showed that oils extracted from JSM by-products can be a good source of oil for application in food, pharmaceutical and cosmetic industries. Therefore, the present research revealed the potentiality of green valorisation of S. niphonius by-products as a possible sustainable approach for targeting the era of zero waste.

Development and evaluation of indigenous snack Promega Khakra enriched with omega-3 rich canola oil and flax seed

There has been an increase in the incidence of life style diseases like, obesity, diabetes mellitus, high blood pressure etc., in our society. Awareness regarding the role of food in causing these lifestyle diseases has also increased among the population. Print media and social media have also started highlighting the ill effects of poor food choices. In general people have become health conscious and acknowledge the importance of healthy food when compared to the easily available junk and fast food. The Corona pandemic has also increased the demand for healthy nutrition. Therefore, there is a need to develop products which will meet the demands of this ever-growing health conscious population. These newer products should be preservative free, natural and should also have health benefits. High levels of Low-density lipoprotein (LDL) cholesterol in an individual has been associated with a wide range of cardiovascular diseases. Omega-3 fatty acids play a significant role in lowering high LDL cholesterol levels. Omega-3 fatty acids also improve cognitive functions among all age groups. There are very few vegetarian foods which provide omega-3 fatty acids. Khakra is a popular traditional Gujarati snack prepared mainly from wheat flour. An attempt has been made to develop a healthy khakra enriched with protein and omega-3 fatty acids (Promega khakra). Various flours like, wheat flour, gram flour, jowar flour and seeds like flax seeds and chia seeds were used in the development of this healthy enriched khakra. Canola oil was used in the preparation of the enriched khakra. Different compositions and combinations were tried to arrive at an acceptable product. These variations were evaluated for their acceptability using a 9 point hedonic scale by 25 semi trained panelists. Sensory evaluation and statistical analysis revealed that khakras (V-1) made with the following ingredients wheat flour (32%), ground flax seeds (19%), jowar flour (6%), gram flour (6%), canola oil (23%), kasuri methi (5%), cumin seeds (3%), turmeric powder (2%), red chilli powder (2%) and salt (2%) was well accepted (7.64 ± 0.71) . Nutritional composition of the standardised khakra showed the following per 100gms: Energy 410.88 kcals, carbohydrates 49.66 g, protein 12.24 g, fat 22.85g, total fiber 9.38g, moisture 1.56 per cent, ash 4.31 percent. The omega-3 content was 2.6 per 100gm (2 promega khakras) which meets approximately 3/4th of the daily requirement of an adult. The healthy Promega khakras thus developed can be consumed by all age groups.

Co-encapsulation of Lactobacillus acidophilus (La-5) and omega-3 rich oil through complex coacervation

Co-encapsulation of Lactobacillus acidophilus (La-5) and omega-3 rich oil through complex coacervation

Microencapsulation of omega-3 rich flaxseed and fish oils

Omega-3 fatty acids can be widely considered as potential therapeutic agents due to their antioxidant, nutritional, and health aspects. They have unsaturated nature which leads to susceptibility to oxidation conditions. Therefore, to get the maximum health benefit and to minimize or prevent the process of oxidation, omega-3 rich oils must be protected against atmospheric oxygen. To achieve this objective, two strategies were usually carried out: First addition of an antioxidant to the oils, and second, the encapsulation process. Here we review omega-3 fatty acids chemistry, natural sources, health benefits, and antimicrobial activity. This review highlights obstacles to incorporating omega-3 fatty acids in foods, particularly concerning oxidation. The importance of incorporating natural polyphenols especially curcumin as well as using different wall materials (sodium alginate, chitosan, and natural clays) in microencapsulating fish and flaxseed oils were also discussed. Different techniques of encapsulation were highlighted as well.

Omega-3 Rich Oils Attenuate ADHD-Like Behaviour Induced by Dietary Monosodium Glutamate in Rats.

&lt;b&gt;Background and Objective:&lt;/b&gt; Attention Deficit Hyperactivity Disorder (ADHD) is a neurodevelopmental disorder characterized by inattention, hyperactivity and cognitive dysfunction. The present study was designed to examine the possible modulatory effect of Fish, Walnuts or Fenugreek Oils against Attention Deficit Hyperactivity Disorder (ADHD)-like Behavior induced by Monosodium Glutamate (MSG) in Rats. &lt;b&gt;Materials and Methods:&lt;/b&gt; Fifty weaning rats were divided into five groups, (each group contain 10 rats) as follows: Group 1: Normal control rats were fed on a balanced diet. Groups from 2-5 rats were fed on a balanced diet+MSG (0.4 g kg&lt;sup&gt;&lt;/sup&gt;&lt;sup&gt;1&lt;/sup&gt; diet), Group 2 served as a positive control group whereas group 3, 4 and 5 treated with Fish, Walnuts and Fenugreek oil, respectively, (200 mg kg&lt;sup&gt;&lt;/sup&gt;&lt;sup&gt;1&lt;/sup&gt; b.wt.) by intra-gastric tube. Biochemical and behavioural parameters were tested as well as microscopic examination of brain tissue was done. &lt;b&gt;Results:&lt;/b&gt; MSG ingestion caused marked disruption in locomotors activity, memory function and brain tissue structure along with significant abnormalities in some bio-markers and reduction in the gene expression level of Bcl-2 in brain tissue. However, treatment with the tested oils showed remarkable effect by reversing the condition. &lt;b&gt;Conclusion:&lt;/b&gt; Dietary supplementation with walnut; fenugreek or fish oils at the tested dose could modulate the condition of ADHD in rats.

Buttermilk as Encapsulating Agent: Effect of Ultra-High-Pressure Homogenization on Chia Oil-in-Water Liquid Emulsion Formulations for Spray Drying.

Functional foods are highly demanded by consumers. Omega-3 rich oil and commercial buttermilk (BM), as functional components, used in combination to produce emulsions for further drying may facilitate the incorporation to foods. Ultra-high-pressure homogenization (UHPH) has a great potential for technological and nutritional aspects in emulsions production. The present study aimed to examine the potential improvement of UHPH technology in producing buttermilk-stabilized omega-3 rich emulsions (BME) for further drying, compared with conventional homogenization. Oil-in-water emulsions formulated with 10% chia: sunflower oil (50:50); 30% maltodextrin and 4 to 7% buttermilk were obtained by using conventional homogenization at 30 MPa and UHPH at 100 and 200 MPa. Particle size analysis, rheological evaluation, colloidal stability, zeta-potential measurement, and microstructure observations were performed in the BME. Subsequent spray drying of emulsions were made. As preliminary approximation for evaluating differences in the homogenization technology applied, encapsulation efficiency and morphological characteristics of on spray-dried emulsions (SDE) containing 21.3 to 22.7% oil content (dry basis) were selected. This study addresses the improvement in stability of BME treated by UHPH when compared to conventional homogenization and the beneficial consequences in encapsulation efficiency and morphology of SDE.

Apparent Digestibility of Macronutrients and Fatty Acids from Microalgae (Schizochytrium sp.) Fed to Rainbow Trout (Oncorhynchus mykiss): A Potential Candidate for Fish Oil Substitution.

Simple SummaryAquaculture is the world’s fastest growing sector of the global food system. Aquaculture feed producers are seeking substitutes for fishmeal and fish oil to develop more sustainable feeds. Marine microalgae show promise as potential ingredients in aquafeeds; however, the literature lacks data on the digestibility and availability of macronutrients and individual fatty acids for omega 3-rich microalgal biomass, Schizochytrium spp., in rainbow trout. This is an important gap to fill because, among marine microalgae, this species is the most frequently used in fish feed, particularly as a dietary supplement or mixed with other ingredients; digestibility data would guide more judicious inclusion of this microalga biomass. High digestibility of macronutrients, energy and fatty acids showed that Schizochytrium spp. is a high-quality substitute for fish oil and potential candidate as a supplement of LC-PUFA in trout feed with vegetable oils. Digestibility is influenced by temperature and several trials have shown increased macronutrient digestibility with increasing temperature. There is an urgent need to determine the effect of temperature on lipid and fatty acid digestibility. We found that the digestibility of nutrients in rainbow trout maintained at 8 °C or 15 °C was not significantly different. However, the digestibility of DHA omega 3 was significantly higher at 8 °C than at 15 °C.Aquaculture feed formulation has recently turned its focus to reduce the reliance on marine-derived resources and utilise alternative feedstuffs, as an approach to improve the environmental sustainability of the aquaculture sector. The fish oil market is highly volatile, and availability of this commodity is continuously decreasing for use in aquaculture. Currently, a growing number of commercial efforts producing microalgae are providing omega 3-rich oil for sustainable aquaculture feed. This study was focused to determine the nutrient digestibility of a marine microalga, Schizochytrium spp., which is rich in docosahexaenoic acid (DHA) and long-chain polyunsaturated fatty acids (LC-PUFA), as a novel dietary lipid source that could be utilized effectively by rainbow trout (Oncorhynchus mykiss). A whole-cell Schizochytrium spp. biomass was used in the digestibility experiment at two different temperatures, 8 °C and 15 °C. No significant differences were detected between the two temperatures for the apparent digestibility coefficients (ADCs) of the dry matter (94.3 ± 4.9%), total lipids (85.8 ± 0.0%), crude proteins (89.5 ± 1.8%), energy (83.1 ± 1.7%) and fatty acids (85.8 ± 7.5%). The ADCs of the nutrients, energy, DHA and other fatty acids showed that Schizochytrium spp. is a high-quality candidate for fish oil substitution and supplement of LC-PUFA in fish feed with vegetable oils.

Transgenerational supplementation with eicosapentaenoic acid reduced the metabolic consequences on the whole body and skeletal muscle in mice receiving an obesogenic diet.

The effect of manipulating the fatty acid profile of the diet over generations could affect the susceptibility to develop obesity and metabolic disorders. Although some acute effects were described, the impact of transgenerational continuous supplementation with omega 3 fatty acids on metabolic homeostasis and skeletal muscle metabolic flexibility during a nutritional stress is unknown. We analyzed the effect of an obesogenic diet in mice after transgenerational supplementation with an omega-3 rich oil (mainly EPA) or a control oil. Young F3 animals received a high fat and high sucrose diet for 4months. Whole-body biometric data were recorded and lipidomic/transcriptomic adaptations were explored in the skeletal muscle. F3 mice from the lineage supplemented with EPA gained less weight, fat mass, and exhibited better metabolic parameters after the obesogenic diet compared to mice from the control lineage. Transcriptomic exploration of skeletal muscle showed differential regulation of biological processes such as fibrosis, fatty acid catabolism, and inflammation between lineages. These adaptations were associated to subtle lipid remodeling of cellular membranes with an enrichment in phospholipids with omega 3 fatty acid in mice from the EPA lineage. Transgenerational and continuous intake of EPA could help to reduce cardiovascular and metabolic risks related to an unbalanced diet by the modulation of insulin sensitivity, fatty acid metabolism, and fibrosis in skeletal muscle.