Omega-3 Fatty Acid Content Research Articles

Fortification of yogurt with flaxseed powder and evaluation of its fatty acid profile, physicochemical, antioxidant, and sensory properties

Flaxseed is a natural ingredient with health benefits because of its rich contents of omega-3 fatty acids, phenolic compounds, and fiber. In this study, yogurt samples enriched with flaxseed powder were produced to achieve the potential health benefits of flaxseed. The results showed that the addition of flaxseed powder significantly increased pH, acidity, water holding capacity, and viscosity of yogurts. This enrichment led to a significant decrease (P < 0.05) in saturated fatty acids (SFAs), the ratio of omega-6 to omega-3 fatty acids, and atherogenic indices of yogurts and an increase in the polyunsaturated fatty acids (PUFAs). In addition, the enriched yogurts had a higher antioxidant activity compared to the controls. The color tests revealed that there was a statistically significant difference in color attributes (except a* values) between the samples (P < 0.05). Finally, the organoleptic characteristics of enriched yogurts were acceptable.

Supplementation with the omega-3 long chain polyunsaturated fatty acids: Changes in the concentrations of omega-3 index, fatty acids and molecular phospholipids of people at ultra high risk of developing psychosis

Omega-3 long chain polyunsaturated fatty acids (n-3 LCPUFA) are necessary for optimum mental health, with recent studies showing low n-3 LCPUFA in people at ultra-high risk (UHR) of developing psychosis. Furthermore, people at UHR of psychosis had increased erythrocyte sphingomyelin (SM) and reduced phosphatidylethanolamine (PE) concentrations as well as 27 erythrocyte phospholipid species that differed when compared to erythrocytes from age matched people not at UHR of psychosis. The aim of this analysis was to evaluate the effect of n-3 supplementation on the different erythrocyte lipid species (including SM and PE concentrations) in people at UHR of psychosis. Participants were randomly assigned to fish oil (containing 840 mg EPA and 560 mg DHA per day) or placebo (paraffin oil) for 6 months. Fasted blood samples were taken at baseline and post intervention. Mass spectrometry was used to analyse the molecular phospholipids and fatty acid composition of erythrocytes for both groups. The n-3 index was significantly increased from 3.0% to 4.12% after 6 months of receiving n-3 capsules. Fish oil capsules increased the phospholipid molecular species containing n-3 LCPUFA, and concomitant decreases in n-6 LCPUFA species. SM species did not show any significant changes in n-3 LCPUFA group however, three SM species (SM 16:0, SM 18:0, SM 18:1) significantly increased after 6 months of supplementation with placebo. N-3 supplementation for 6 months led to higher n-3 incorporation into erythrocytes, at the expense of n-6 PUFA across all phospholipid classes analyzed and may have prevented the increase in SM seen in the placebo group.

Enrichment of omega-3 fatty acids, waste oil by-products canning tuna (thunnus sp.) with urea crystallization

Enrichment test of omega-3 fatty acids from tuna canning waste oil in Bitung North Sulawesi Indonesia have been conducted. The purpose of this study was to utilize tuna canning waste oil and enriched the concentration of omega-3 fatty acids with urea crystallization. The results showed that the characteristics of fish oil quality of tuna canning waste 0.9% moisture content, free fatty acids (FFA) 3.4%, copper (Cu) 0.1 ppm, iron (Fe) 2.5 ppm and phosphorus (P) 32,5 ppm still meets IFOMA quality standards Liquid fatty acid profile contains total SFA 33.7%, 27.6% PUFA, 1.4% omega-6 fatty acids, omega-3 fatty acids 25.9%, EPA 4.2% and DHA 21.4%, MUFA 18.8%, omega-9 fatty acids 13.9%, while concentrations of omega-3 fatty acids contained a total SFA of 7.7%, PUFA 70.9%, omega-6 fatty acids 0.8%, omega-3 fatty acids 70.1%, EPA 4.2% and DHA 21.4%, MUFA 2.7%, omega-9 fatty acids 1.5%. The urea crystallization process increases the levels of polyunsaturated fatty acids (PUFAs) such as omega-3 (EPA and DHA.), the enrichment rate is 2.7 times.

1.37 OMEGA-3 SUPPLEMENTATION FOR ASD

ASD is a neurodevelopmental condition characterized by persistent deficits in social communication and social interaction, as well as restricted, repetitive patterns of behavior, interests, and activities. In 2010, it was estimated that the global prevalence of ASD was 7.6 per 1000 (1 in 132). It also is known that the presence of reduced concentration of omega-3 fatty acids has been reported in patients with ASD. The use of omega-3 in ASD has been studied for the symptomatic treatment of the condition.

Canola Breeding for HUMAN HEALTH

Canola Breeding for HUMAN HEALTH

Increased Maternal Prenatal Adiposity, Inflammation, and Lower Omega-3 Fatty Acid Levels Influence Child Negative Affect.

ObjectiveIncreased maternal adiposity during pregnancy is associated with offspring risk for psychiatric disorders. Inflammation secondary to adiposity is believed to be an important mechanism through which this effect occurs. Although increased adiposity introduces risk, not all children of overweight mothers develop these problems. Gestational factors that modify this risk are not well-understood. If maternal increased adiposity exerts its effects on offspring outcomes by increasing inflammation in the gestational environment, then anti-inflammatory inputs such as omega-3 fatty acids may be one protective factor. The goal of this study was to investigate whether maternal pre-pregnancy body mass index (BMI) and omega-3 fatty acid levels independently and/or interactively predicted offspring infant negative affect, an early life marker of risk for psychopathology.MethodsData came from a prospective study of women recruited during pregnancy and their 6 month old infants (N = 62; 40% female). Maternal pre-pregnancy BMI was pulled from medical charts and third trimester omega-3 fatty acid concentrations were assessed in plasma. Child negative affect was assessed using observer- and maternal-ratings at 6 months of age. Maternal inflammation was indexed by third trimester plasma levels of interleukin-6, tumor necrosis factor-alpha, and monocyte chemoattractant protein-1.ResultsMaternal pre-pregnancy BMI was associated with increased infant negative affect whereas eicosapentaenoic acid was associated with less infant negative affect. Maternal omega-3 fatty acid levels moderated the effect of BMI on infant negative affect, such that omega-3 fatty acids buffered children against the negative consequences of increased adiposity. Supporting the role of maternal inflammation in these associations, maternal BMI and omega-3 fatty acid levels interacted to predict maternal third trimester inflammation. Further, maternal inflammation was associated with increased infant negative affect.ConclusionResults suggest that omega-3 supplementation during pregnancy may protect against offspring behavioral risk associated with increased maternal adiposity.

Harnessing global fisheries to tackle micronutrient deficiencies

Micronutrient deficiencies account for an estimated one million premature deaths annually, and for some nations can reduce gross domestic product1,2 by up to 11%, highlighting the need for food policies that focus on improving nutrition rather than simply increasing the volume of food produced3. People gain nutrients from a varied diet, although fish-which are a rich source of bioavailable micronutrients that are essential to human health4-are often overlooked. A lack of understanding of the nutrient composition of most fish5 and how nutrient yields vary among fisheries has hindered the policy shifts that are needed to effectively harness the potential of fisheries for food and nutrition security6. Here, using the concentration of 7nutrients in more than 350 species of marine fish, we estimate how environmental and ecological traits predict nutrient content of marine finfish species. We use this predictive model to quantify the global spatial patterns of the concentrations of nutrients in marine fisheries and compare nutrient yields to the prevalence of micronutrient deficiencies in human populations. We find that species from tropical thermal regimes contain higher concentrations of calcium, iron and zinc; smaller species contain higher concentrations of calcium, iron and omega-3 fatty acids; and species from cold thermal regimes or those with a pelagic feeding pathway contain higher concentrations of omega-3 fatty acids. There is no relationship between nutrient concentrations and total fishery yield, highlighting that the nutrient quality of a fishery is determined by the species composition. For a number of countries in which nutrient intakes are inadequate, nutrients available in marine finfish catches exceed the dietary requirements for populations that live within 100kmof the coast, and a fraction of current landings could be particularly impactful for children under 5years of age. Our analyses suggest that fish-based food strategies have the potential to substantially contribute to global food and nutrition security.

Experimental evaluation of the culture parameters for optimum yield of lipids and other nutraceutically valuable compounds in Chloroidium saccharophillum (Kruger) comb. Nov

The progress of algal-based biofuel industry depends on the identification of high lipid-yielding algal species from diverse environments. Laboratory-scale standardization of culture requirements for optimum yield of lipids and other desirable chemicals are highly important in the industrial utilization of an algal species. The major objectives of the present study were such an evaluation of the industrial potentials of a fast-growing native green microalga isolated from eutrophicated water bodies of Kerala, South India, morphologically and molecularly identified as Chloroidium saccharophilum. Biomass productivity and biochemical composition of the alga in relation to culture parameters such as total carbohydrates, proteins, pigments and lipids were learned. At an increased pH (8) or salinity (50 mM) and at a deprived nitrate (0 mM) or decreased phosphate (0.5 mM) in the medium, the alga was found producing lipids that have good biodiesel properties such as low iodine value and high cetane number. Optimum culture parameters such as pH, salinity, nitrate and phosphate conditions for maximum biomass yield of the alga are discovered. It was found that an increase in salinity or phosphates and a decrease in nitrate from that of the normal Bold's Basal Medium are favourable for an increase in omega-3 fatty acid content in the lipid.

Maternal polymorphisms in the FADS1 and FADS2 genes modify the association between PUFA ingestion and plasma concentrations of omega-3 polyunsaturated fatty acids

There is a lack of studies about polymorphisms in FADS genes in pregnant women. We aimed to verify the interaction between three FADS gene polymorphisms (rs174561; rs174575; rs3834458) and dietary α-linolenic acid (ALA) or linoleic/α-linolenic acid ratio (LA/ALA) and plasma concentrations of omega-3 (n−3) PUFAs in pregnant women. Of the 250 women evaluated, the homozygous for the rs174561 and rs3834458 minor allele had high plasma ALA concentrations at the highest ALA and LA/ALA ratio tertile (p < 0.05). Plasma concentrations of EPA and DHA were not influenced by diet. For the rs174575 SNP, pregnant women who carried the minor allele presented lower proportions of plasma EPA in the second LA/ALA ratio tertile (p < 0.05). Increased dietary intake of ALA and LA/ALA ratio promoted plasma ALA accumulation in homozygotes for the minor allele rs174561 and rs3834458. Moderate intake of LA/ALA ratio may reduce plasma concentration of EPA in pregnants carrying the rs174575 minor allele.

GWAS identifies genetic variants associated with omega-3 fatty acid composition of Atlantic salmon fillets

The objective of this study was to identify genetic variants associated with long-chain omega-3 fatty acid content in Atlantic salmon muscle, in order to identify genes underlying the genetic variation in these traits, by performing a genome-wide association study.Fatty acid composition, including eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA) content and the ratios DHA/DPA (docosapentaenoic acid) and DHA/ALA (α-linolenic acid) of skeletal muscle was determined in 642 Atlantic salmon individuals from the Salmobreed broodstock program. Further, a 57 K single-nucleotide polymorphism (SNP) array was used to genotype the 642 individuals to search for SNPs associated with skeletal muscle content of individual omega-3 fatty acids as well as ratios between fatty acids, using a mixed model approach. We identified markers showing a significant association with the ratio of DHA to DPA located on chromosome 19, close to the candidate gene elovl2, which is directly involved in the conversion of DPA to DHA. The results further suggested that genetic variation affecting fillet EPA and DHA content is present on Atlantic salmon chromosome 21, as the GWAS analysis of EPA, DHA and DHA/ALA ratio all pointed to this chromosome. No known genes of direct relevance to the omega-3 bioconversion pathway were found here. We discuss the relevance of other interesting genes related to lipid metabolism and health located in this region.

Fatty Acid Profile, Total Carotenoids, and Free Radical-Scavenging from the Lipophilic Fractions of 12 Native Mexican Avocado Accessions.

Mexico is the world's largest producer, exporter, and consumer of avocados. "Hass" avocado is the most commercialized cultivar, while the native Mexican avocado varieties have been displaced. Thus, studies regarding their chemical and nutraceutical characterization are scarce. We studied the total lipid content, fatty acid profile, carotenoid content, and free radical-scavenging activity of the fruit pulp from 12 accessions of the native Mexican avocado (Persea americana var. drymifolia). The results show that the chemical and nutraceutical characteristics depend on the accession type. The total lipid content ranged from 13.22 to 23.41%. The major saturated fatty acid in all the avocado accessions was palmitic acid (15.54-22.68%). Monounsaturated fatty acids, like oleic (56.58-74.19%), linoleic (5.62-16.85%) and linolenic (0.63-2.85%) acids were the most abundant unsaturated fatty acids in all the native Mexican avocado accessions. The total carotenoid content (1.57 to 7.83mgeq. of β-carotene 100g-1 fresh weight) and the free radical-scavenging activity (7.58-88.62 mMol trolox equivalent 100g-1 fresh weight) also varied significantly (p < 0.05) among accessions. Native Mexican avocados have a great nutraceutical potential due to their high content of omega-9, omega-6, and omega-3 fatty acids and carotenoids. These compounds have been reported to display antioxidant activities and protect against cardiovascular diseases.

Serum omega-3 and omega-6 fatty acid concentrations and fecundity

Omega fatty acid serum concentrations have been shown to play an important role in reproduction in animal models, with conflicting results in human studies. We sought to determine the association between serum omega-3 and omega-6 fatty acid concentrations and the probability of conceiving, the risk of miscarriage, and ovarian reserve.

The Effect of Phytase on Production Performance, Egg Quality, Calcium and Phosphorus Excretion, and Fatty Acids and Cholesterol Concentration in Hy-Line Layers Fed Diets Containing Rice Bran

SUMMARY According to low availability of phosphorus in plant protein sources like rice bran, the importance of phosphorus requirement in poultry nutrition especially layers is increasing. This experiment was conducted to investigate the effects of phytase in diets containing different levels of rice bran on production performance, egg quality, and fatty acids and cholesterol concentration which affects the nutritional value of layer eggs. A total of 120 62-wk-old white Hy-line W-36 layer hens had corn-soybean diets with different levels of rice bran (0, 15, and 25%) and with or without phytase supplementation (250 FTU/kg). The eggshell weight and thickness were higher in phytase supplemented group. Haugh unit was not significantly different between experimental groups. The highest egg and yolk weight belonged to a treatment group supplemented with 25% rice bran. Inclusion of 25% rice bran increased egg weight, but decreased hen house egg production; hence, the egg mass did not show any significant changes. Cholesterol concentration of eggs in a group with 25% rice bran decreased, but fatty acids, omega-3, and omega-6 concentration increased significantly in the experimental diets supplemented with rice bran. Phytase supplemented groups had a significant decrease in the excretion of P and Ca which reduced the environmental concerns attributed to P excretion. In conclusion, the layers fed 250 FTU/kg phytase supplemented with 25% rice bran had improved performance, egg composition, and shell quality.

The Fatty Acid Composition and Quality of Oils from Post‐Industrial Waste of Quince Chaenomeles japonica

In the food industry, quince seeds are discarded as waste in the production process. Their use therefore creates added value and opens up the possibility of using no-waste processing technologies. Three types of waste were investigated: after juicing, after the manufacture of puree and syrup. The results showed that the yield of quince seeds (Chaenomeles japonica (Thunb.) Lindl. ex Spach from waste left after different production methods varies from 29.8 to 38.3 %. The cold pressed oil yield ranges from 4.9±0.03 to 7.1±0.06 %. The oil yield obtained by Soxhlet extraction varies from 14.6±0.64 to 17.3±0.9 %. Unsaturated fatty acid, especially polyunsaturated fatty acid is predominant in quince seed oil. The linoleic acid content of the quince seed oils was between 47.12 % and 58.49 % of the total fatty acids. The fatty acid composition of oils from post-industrial waste is more appropriate in the skin care industry than in the food industry because of the high ratio of omega-6/omega-3 and high linoleic acid content.

IDENTIFICATION OF PHYTOPLANKTON TYPES IN TAMBAK BANDENG WITH HIGH QUALITY OF OMEGA-3

Microalgae as a source of nutrients, can be a good alternative to the use of high-level organisms. The abundant omega 3 content in plankton, especially phytoplankton, needs to be conducted research on the identification of the types of phytoplankton in milkfish with high omega-3 quality. The purpose of the study was to identify the types of plankton containing omega-3 and to determine the levels of omega-3 from plankton found in milkfish ponds. This research was conducted at the Laboratory of the Faculty of Fisheries and Marine Sciences, Brawijaya University, Malang. The highest abundance of phytoplankton is from the Bachillariophyta division and the lowest division euglenophyta. Five types of plankton were determined to be tested for omega-3 fatty acids using the gas chromatography (GCMS) method, namely: Chaetoceros sp, Tetraselmis sp, Nitzchia, Chlorella and Spirulina. GCMS test results from several types of omega-3 fatty acid plankton isolates in Chaetoceros calcitrans were the highest compared to the other 4 plankton types. Conclusion of the composition of plankton in traditional pond waters consists of 4 phytoplankton divisions namely Chlorophyta, Chrysophyta, Cyanophyta, and Euglenophyta. GCMS test results from several types of plankton isolates omega-3 fatty acids in Chaetoceros sp, the highest compared to Tetraselmis sp, Nitzchia , Chlorella and Spirulina. Suggestion of natural feed in the water of milkfish ponds should use Chaetoceros sp because the content of omega 3 is high. Keywords: phytoplankton, omega-3

Quality Traits of Fillet of Gilthead Sea Bream (Sparus aurataL.) of Two Market Sizes Reared according to an “Antibiotic-Free” Approach

Rearing of “antibiotic-free” fish is becoming one of the most important goals to be achieved in the near future. Strategies are going to be adopted to increase nonspecific immune system and biosecurity in fish farming. To show the quality traits of gilthead sea breams reared under a prescription of “antibiotic-free” (ABF), two different market sizes (R: about 350 g andM: about 490 g) were analysed at the end of the farming cycle performed in a floating cage plant in the Adriatic sea, in the Gulf of Valona (Albania). Proximate composition and fatty acid profile were determined in April and June 2018. The omega-3 content demonstrated that the ABF samples collected according to the fish size and the harvest month can be defined as “rich in omega-3,” in compliance with the Annex to the Regulation (EC) n. 1924/2006, with a trend to increase in relation to fish growth.

Dietary chia seeds (Salvia hispanica) improve acute dyslipidemia and steatohepatitis in rats.

Chia seeds (Salvia hispanica L.) are rich in omega fatty acids. Dyslipidemia and steatohepatitis are diseases that require effective treatments in obese and non-obese patients. The aim was to evaluate the effect of chia intake on acute tyloxapol (TI)-induced dyslipidemia, on acute carbon tetrachloride (TC)-induced steatohepatitis, and on mixed damage (TC+TI) in non-obese rats. Four experimental groups were fed for 4weeks a diet with established rodent food (DE), and four groups were fed a diet with 15% added chia (DC). Plasma samples were analyzed for total cholesterol, triglycerides, glucose, biochemical liver damage markers, and tumor necrosis factor-α (TNF-α). Liver samples were used to quantify glycogen, catalase, lipid peroxidation, and TNF-α. A histopathological analysis was performed. DC intake partially or totally prevented steatohepatitis, and reduced lipids in the dyslipidemic groups. The hypolipidemic and hepatoprotective effects of chia may be correlated to its high content of α-linolenic acid (omega-3) and phenolics. PRACTICAL APPLICATIONS: Metabolic syndrome is associated with non-alcoholic fatty liver disease (NAFLD) and non-alcoholic steatohepatitis (NASH), which are currently the most common causes of chronic liver disease, cirrhosis, and hepatocellular carcinoma (HCC) worldwide. Dyslipidemia is a significant risk factor for NAFLD and NASH. Non-obese patients may have NAFLD or NASH. Metabolic syndrome and dyslipidemia are more strongly associated with NAFLD in non-obese than in obese patients. This is the first study evaluating the hypolipidemic and hepatoprotective effects of chia seed intake on acute dyslipidemia and/or steatohepatitis caused by the individual or combined administration of the inducers tyloxapol and carbon tetrachloride, respectively, in non-obese rats. The pharmacological effects of dietary chia are correlated to its high content of omega-3 and omega-6 (1:1), protein, dietary fiber, and phenolics. The results suggest that inclusion of chia in diets of non-obese patients with dyslipidemia and/or NAFLD/NASH may improve their health state and preventing cirrhosis or HCC.

Suckler Bulls Slaughtered at 15 Months of Age: Effect of Different Production Systems on the Fatty Acid Profile and Selected Quality Characteristics of Longissimus Thoracis.

The objective was to compare the quality of beef from bulls reared in typical Irish indoor systems or in novel grass-based systems. Bulls were assigned to one of the following systems: (a) grass silage plus barley-based concentrate ad libitum (CON); (b) grass silage ad libitum plus 5 kg of concentrate (SC); (c) grazed grass without supplementation (G0); (d) grazed grass plus 0.5 kg of the dietary dry matter intake as concentrate (GC) for (100 days) until slaughter (14.99 months). Carcass characteristics and pH decline were recorded. Longissimus thoracis was collected for analytical and sensory analysis. Lower carcass weight, conformation and fatness scores were found for grazing compared to CON and SC groups. CON bulls had highest intramuscular fat and lighter meat colour compared with grazing bulls. The SC meat (14 days aged) was rated higher for tenderness, texture, flavour and acceptability compared with grazing groups. CON saturated and monounsaturated fatty acid (FA) concentration was highest, conversely, omega-3 FA concentration was higher for GC compared with CON, while no differences were found in polyunsaturated FA. In conclusion, while market fatness specification was not reached by grazed grass treatments, beef eating quality was not detrimentally affected and nutritional quality was improved.

Application of genome editing in aquatic farm animals: Atlantic salmon.

Gene editing offers opportunities to solve fish farming sustainability issues that presently hampers expansion of the aquaculture industry. In for example Atlantic salmon farming, there are now two major bottlenecks limiting the expansion of the industry. One is the genetic impact of escaped farmed salmon on wild populations, which is considered the most long-term negative effect on the environment. Secondly and the utmost acute problem is the fish parasite salmon lice, which is currently causing high lethality in wild salmonids due to high concentrations of the parasite in the sea owing to sea cage salmon farming. There are also sustainability issues associated with increased use of vegetable-based ingredients as replacements for marine products in fish feed. This transition comes at the expense of the omega-3 content both in fish feed and the fish filet of the farmed fish. Reduced fish welfare represents another obstacle, and robust farmed fish is needed to avoid negative stress associated phenotypes such as cataract, bone and fin deformities, precocious maturity and higher disease susceptibility. Gene editing could solve some of these problems as genetic traits can be altered positively to reach phenotype of interest such as for example disease resistance and increased omega-3 production.

Effect of grazing cows in an intensive silvopastoral system with Leucaena leucocephala on Panela cheese properties: yield, composition, fatty acid profile and sensory acceptability.

Yield, chemical composition, fatty acid profile, and sensory acceptability of Panela cheese produced from cows grazing in an intensive silvopastoral system (ISS) with Leucaena leucocephala and Cynodon nlemfuensis were evaluated and compared with Panela cheese from cows grazing a monoculture system (MS) of C.nlemfuensis only. The experiment lasted for 9weeks in a tropical area in Mexico using ten crossbred cows (30-90days of milking) assigned homogenously as five cows in each experimental group. No significant differences were found between the two systems for milk and cheese gross composition. Panela cheese from ISS showed lower content of the hypercholesterolemic fatty acids, accompanied with higher content of omega-3, omega-6 and polyunsaturated fatty acids. In addition, Panela cheese from ISS showed higher preference for the attributes of appearance, texture, flavor, and overall acceptability. It is concluded that Panela cheese from cows grazing in ISS has better acceptability and nutritional properties than that produced from MS with grass only.