Roots Of Olive Trees Research Articles

Olive (Olea europaea L.) Root Growth in Soil Sectors with Olive Husks and Hay Residues

ABSTRACT Olive oil by-products have an important fertilizing value due to their high organic matter and nutrient content; however, they also possess a phytotoxic effect. Therefore, it is necessary to study the reaction of plants and, more specifically, the roots of olive trees to the presence of husks used as amendment in olive orchards. In this experimental work olive plants were grown in split-pots in which the roots were divided in four sectors and grown in different substrates. Husks, hay, and a mixture of the two was added to a soil substrate to simulate growing conditions in an olive orchard with green mulching. Results showed that olive roots performed differently in the different substrates. Husk substrates were avoided if a husk-free section was present. If not, the plants stopped growing and half of them perished. If hay was added with husks the toxic effect was reduced. It can be argued that in the field, olive plants, thanks to their great transmigration capacity, may be able to avoid the patches amended with husks, but repeated treatments with husks could cause shoot growth problems because it would be more difficult for roots to find optimal substrate to continue their growth.

Water balance of the olive tree–annual crop association: A modeling approach

Water transfers within mixed crops systems are complicated to understand due to the large number of complex interactions between the various components. Standard techniques fail to provide the proper assessment of the components of the water balance. Experiments and modeling developments are used to understand the dynamics of water transfers within the association of olive trees with annual crops under irrigation in Central Tunisia. The whole system is represented by a unit area made up of three components: a plot with the annual crop, a plot with the olive tree and a plot of bare soil. The modeling approach is based on the concept of reservoir. The model works on a daily time step and accounts for the lateral transfers of water occurring between the components of the system: (i) the water uptake by the roots of olive trees; (ii) the physical flow of water between the irrigated plot and the non-irrigated ones. A field experiment was carried out during 2 years (2002, 2003) and three crop cycles (spring potato, spring pea and autumn potato) in order to calibrate the model and test its validity. Olive tree transpiration was estimated from sap flow measurements and soil moisture in the different compartments was measured by neutron probe technique. The experimental data compare fairly well with the model outputs. The first purpose of the model is to understand the functioning of the olive tree–annual crop association from a water standpoint, but it can be easily extended to other intercropping systems mixing perennial vegetation with annual crops or used as a management tool. The estimates of the water extracted by the olive trees in each reservoir appear to be much more significant than those of the water physically transferred between reservoirs.

Osmotic regulation in leaves and roots of olive trees during a water deficit and rewatering

We evaluated the osmotic adjustment capacity of leaves and roots of young olive (Olea europaea L.) trees during a period of water deficit and subsequent rewatering. The trials were carried out in Basilicata (40 degrees 24' N, 16 degrees 48' E) on 2-year-old self-rooted olive plants (cv. 'Coratina'). Plants were subjected to one of four drought treatments. After 13 days of drought, plants reached mean predawn leaf water potentials of -0.45 +/- 0.015 MPa (control), -1.65 +/- 0.021 (low stress), -3.25 +/- 0.035 (medium stress) and -5.35 +/- 0.027 MPa (high stress). Total osmotic adjustment increased with increasing severity of drought stress. Trees in the high stress treatment showed total osmotic adjustments ranging between 2.4 MPa at 0500 h and 3.8 MPa at 1800 h on the last day of the drought period. Osmotic adjustment allowed the leaves to reach leaf water potentials of about -7.0 MPa. Active osmotic adjustment at predawn decreased during the rewatering period in both leaves and roots. Stomatal conductance and net photosynthetic rate declined with increasing drought stress. Osmotic adjustment in olive trees was associated with active and passive osmotic regulation of drought tolerance, providing an important mechanism for avoiding water loss.

Lipoxygenase activity and proline accumulation in leaves and roots of olive trees in response to drought stress

The olive tree (Olea europaea L.) is commonly grown in the Mediterranean basin and is able to resist severe and prolonged drought. Levels of proline (PRO) and malondialdehyde (MDA), and the lipoxygenase (LOX) activity were determined in 2-year-old olive plants (cv. 'Coratina') grown in environmental conditions characterized by high temperatures and high photosynthetic photon flux density levels and gradually subjected to a controlled water deficit for 20 days. Before and during the experimental period, leaf and root samples were collected and analysed for PRO and MDA. The levels of PRO increased in parallel with the severity of drought stress in both leaves and roots. Significant increases of LOX activity and MDA content were also observed during the progressive increment of drought stress in both leaf and root tissues. Measurements of transpiration and photosynthetic rate, stomatal conductance and substomatal CO(2) concentration were carried out during the experiment. The accumulation of PRO indicates a possible role of PRO in drought tolerance. The increases of MDA content and LOX activity show that the water deficit is associated with lipid peroxidation mechanisms.

First Report of the Parasitic Plant Orobanche aegyptiaca Infecting Olive.

Broomrapes (Orobanche spp.) are obligatory parasitic plants that infect the root system of vegetables and field crops worldwide resulting in severe damage. Five broomrape species are known as significant parasites of crops in Israel: O. aegyptiaca Pers., O. cernua Loefl., O. cumana Wallr., O. crenata Forssk., and O. ramose L. (1,2). Recently, O. aegyptiaca was found to parasitize roots of young olive trees (Olea europaea) in a 1-year-old plantation located in Esdraelon Valley, Israel (voucher specimens deposited in Newe-Ya'ar Weed Herbarium, Ramat Yishay, Israel). To our knowledge, this is the first time that a tree in general and olive in particular has been reported to serve as host for O. aegyptiaca. Washing the root system clearly verified connections between the parasite and olive roots. Cross sections of an attachment site confirmed the development of functional haustoria. Trees were planted in a field where tomatoes had been previously parasitized by O. aegyptiaca for several years. In April 2001, many O. aegyptiaca plants emerged under each olive tree in a total area of 0.3 ha. Additional emergence of O. aegyptiaca was observed until July 2001. The high level of Orobanche infection did not lead to visible damage in the trees. However, the mature parasite developed massive amounts of seeds, serving to increase the population of O. aegyptiaca in the field.

Identity and Pathogenicity of Two Phytophthora Taxa Associated with a New Root Disease of Olive Trees.

Fifty-two Phytophthora isolates from necrotic roots of olives were characterized. Colony morphologies on carrot-agar medium led us to separate them into two groups: A (36 isolates) and B (16 isolates). The optimum growth temperature for Group A was about 21°C, with slow growth at 30°C. In contrast, Group B isolates had an optimum temperature for growth of 26°C, and grew rapidly at 30°C. Growth rates, sporangial and oogonial characteristics of the Group A isolates conformed to P. megasperma "BHR-type" sensu stricto. This designation was supported by a sequence analysis of their ITS rDNA regions. Colony patterns, sporangial characteristics and temperature-growth relationships of the Group B isolates conformed closely to those of the 'O-group' taxon of Phytophthora. They also conformed to this unusual taxon in their ITS sequence. In addition, Group B isolates were either entirely self-sterile, self-sterile A1s or weakly self-fertile. Pathogenicity tests showed that both taxa were highly aggressive on roots of olive trees. The association of flooding with Phytophthora infection indicates that the previously reported high sensitivity of olive to root asphyxiation may be more properly regarded as root-rot caused by Phytophthora spp.

First Report of Phytophthora palmivora as a Pathogen of Olive in Italy.

Olive (Olea europea L.) is an economically important crop in Italy and is planted on about 1 million ha. The Apulia, Calabria, and Sicily regions of Southern Italy account for about 70% of the production. Many new plantations have been established during the last 10 years. In summer 1999, 1- to 2-year-old olive trees (cv. Carolea) with decline symptoms were observed in new plantations in Catanzaro Province (Calabria). The symptoms associated with the root rot were leaf chlorosis, defoliation, wilting, twig dieback, and eventual plant collapse. In some cases, more than 40% of the trees were affected. A Phytophthora sp. was isolated consistently from rotted rootlets of diseased trees using a selective medium (2). Single-zoospore isolates were obtained from the colonies. The species isolated from olive roots was identified as P. palmivora (E. Butler) E. Butler on the basis of morphological and cultural characters according to Erwin and Ribeiro (1). All isolates produced papillate sporangia, which were elliptical to ovoid, caducous (mean pedicel length = 5 µm), with a length-breadth ratio of 1.8. In addition, some isolates produced subglobose, non-papillate sporangia with a length-breadth ratio ranging from 1.2 to 1.5. Electrophoresis of mycelial proteins on polyacrylamide gels confirmed that all isolates were pure cultures and that they all belonged to the same species. The electrophoretic banding patterns of total soluble mycelial proteins and eight isozymes (alkaline phosphatase, esterase, fumarase, NAD-glucose dehydrogenase, glucose-6-phosphate dehydrogenase, lactate dehydrogenase, malate dehydrogenase, and superoxide dismutase) of all olive isolates were identical to those of two strains of P. palmivora from parlor palm and from pittosporum. Conversely, they were clearly distinct from the electrophoretic banding patterns of reference isolates of P. cactorum (Lebert & Cohn) Schroter, P. capsici Leonian, P. citrophthora (R. E. Sm. & E. H. Sm.) Leonian, and P. nicotianae van Breda de Haan. All isolates of P. palmivora from olive were of the A1 mating type. The pathogenicity of four P. palmivora isolates from olive, two producing only typical and two producing both types of sporangia, was tested in the greenhouse at 18 to 25°C, using 20 1-year-old rooted cuttings of olive cv. Carolea for each isolate. Twenty noninoculated cuttings were used as a control. The inoculum for pathogenicity tests was produced on autoclaved rice grains moistened with V-8 juice. Cuttings were transplanted to pots filled with soil infested with inoculum at 2% (wt/vol). Control plants were grown in pots containing a mixture of soil and 2% autoclaved rice. After transplanting, all pots were flooded once a week for 24 h by plugging the drain hole of the pot. One month after planting, all the plants in infested soil had died and no difference in virulence was observed among the isolates. Control plants remained healthy. P. palmivora was reisolated from the roots of symptomatic plants. Pathogenicity tests were repeated twice with similar results. In a survey of nurseries in Southern Italy, P. palmivora was recorded frequently from roots of young olive trees suggesting that infections originated from nurseries. This is the first report from Italy of P. palmivora on olive. This species has been described recently as a pathogen of olive in Spain (3).

Anatomical Response of Olive Roots to Dry and Irrigated Soils.

Root development was examined in roots of young olive trees (Olea europaea L., cv. Manzanillo^ grown under wet and dry water regimes. Cross sections at sequential positions were observed along the root axis from the apex up to 18 cm from the root tip at intervals of 3 cm. An index was established to facilitate development comparisons. Complete transition to secondary growth was found closer to the apex in the roots grown in dry soil (at 9 cm) than in the roots grown in watered soil (at 12 cm). Up to 5 cm from the root tip, the cortical tissue was wider in the roots grown in dry soil than in watered soil. Based on maturity stage, both total root and central cylinder radii were greater for the dry than the irrigated treatment. No difference between treatments was found in metaxylem vessel diameter.

小豆島に於ける土壤調査並にオリープの根群調査

(1) Soil survey and survey of the development of fruit trees or forest trees were made at some places in the village of Nishi and Shikai, Shodo Gun, Kagawa Prefecture. These places were of two kinds, the one of which was presumed to be tales gloscier or talus and the other was in marked contrast with the former regarding topography and soil profile.(2) Because of presumably the block structure of the soil, the root of olive tree penetrated to a depth of 1 meter in the II Horizon notwithstanding the highly clayey texture of the soil which originated from metamorphic clayslate, in the Oki Island. On the hill of Oe the roots of olive tree penetrated more deeply than in the of Oki Island and the maximum depth was recorded to be 1.45 meters. It was supposed that there is close relation between the root development of olive trees and the soil moisture.(3) The fact that the roots of olive trees in Mr. TAKAHASHI'S olive grove in the village of Nishi penetrated shallowly into the soil was supposed to be a case of the phenomenon due to so-called“Leachy Substrata”.