Olive Oil Extraction Research Articles

Functional compounds from olive pomace to obtain high-added value foods - a review.

Olive pomace, the solid by-product from virgin olive oil extraction, constitutes a remarkable source of functional compounds and has been exploited by several authors to formulate high value-added foods and, consequently, to foster the sustainability of the olive-oil chain. In this framework, the aim of the present review was to summarize the results on the application of functional compounds from olive pomace in food products. Phenolic-rich extracts from olive pomace were added to vegetable oils, fish burgers, fermented milk, and in the edible coating of fruit, to take advantage of their antioxidant and antimicrobial effects. Olive pomace was also used directly in the formulation of pasta and baked goods, by exploiting polyunsaturated fatty acids, phenolic compounds, and dietary fiber to obtain high value-added healthy foods and / or to extend their shelf-life. With the same scope, olive pomace was also added to animal feeds, providing healthy, improved animal products. Different authors used olive pomace to produce biodegradable materials and / or active packaging able to increase the content of bioactive compounds and the oxidative stability of foods. Overall, the results highlighted, in most cases, the effectiveness of the addition of olive pomace-derived functional compounds in improving nutritional value, quality, and / or the shelf-life of foods. However, the direct addition of olive pomace was found to be more challenging, especially due to alterations in the sensory and textural features of food. © 2020 Society of Chemical Industry.

Environmental Assessment of Olive Mill Solid Waste Valorization via Anaerobic Digestion Versus Olive Pomace Oil Extraction

Anaerobic digestion is a promising alternative to valorize agrifood wastes, which is gaining interest under an environmental sustainability overview. The present research aimed to compare anaerobic digestion with olive pomace oil extraction, by using life cycle assessment, as alternatives for the valorization of the olive mill solid waste generated in the centrifugation process with a two-outlet decanter from oil mills. In the case of olive pomace oil extraction, two cases were defined depending on the type of fuel used for drying the wet pomace before the extraction: natural gas or a fraction of the generated extracted pomace. The anaerobic digestion alternative consisted of the production of biogas from the olive mill solid waste, heat and electricity cogeneration by the combustion of the generated biogas, and composting of the anaerobic digestate. The life cycle assessment showed that anaerobic digestion was the best alternative, with a global environmental impact reduction of 88.1 and 85.9% respect to crude olive pomace oil extraction using natural gas and extracted pomace, respectively, as fuel.

Varietal Effect on the Concentration and Anti-Inflammatory Activity of Hydroxytyrosol in French Olive Oils.

Numerous studies have been carried out on the bioactive properties of hydroxytyrosol (HT) in olive oils (OOs), although there are few reports comparing anti-inflammatory activity among different olive varieties or regions of production. The purpose of this study was to investigate the in vitro inflammatory action of HT in extracts of four OO varieties in the Languedoc region of the French Mediterranean. Factors other than cultivar were eliminated, which enabled unambiguous varietal differences to be identified. Purified extracts of OO were obtained using an optimized solid-phase extraction procedure by which only polar compounds were recovered. High performance liquid chromatography-photodiode array detection-tandem mass spectrometry was used to identify and quantify HT and oleacein in the extracts. The total polyphenol concentration ranged from 93.00 mg gallic acid equivalent/kg OO for Picholine to 27 mg gallic acid equivalent for Verdale OOs. The concentrations of HT in Picholine, Olivère, and Lucques varieties were 25.3, 18.8 and 12.1 mg/kg, respectively, whereas the concentration of HT in Verdale OOs was less, 1 mg/kg. The in vitro anti-inflammatory response of purified OO extracts, evaluated by the inhibition of nitric oxide release in lipopolysaccharide-induced interferon-γ activated J774.A1 macrophages, strongly correlated with total polyphenol content (R2 > 0.995). The effect increased asymptotically between the equivalent of 2 and 37 mg of OO, reaching, at the maximum tested concentration, 90%, 75%, 62%, and 30% activity for Picholine, Olivière, Lucques, and Verdale, respectively. The results presented here clearly show that, by comparison with authentic standards, the activity of HT in OO extracts was enhanced in a concentration-dependent manner, varying from 3-fold at the highest extract concentration to over 6.5-fold at the lowest extract concentration. Therefore, the anti-inflammatory activity of OOs should be rationalized on the basis of whole extracts rather than solely on the concentration of HT or other bioactive compounds in OO.

Antioxidant, antiradical and antipyretic effects of olive oil extract of propolis

This study was carried out to determine the antioxidant, antiradical and antipyretic effects and the total phenolic content of poplar-type olive oil extract of propolis (OOEP). As a result of the analysis of olive oil extract of propolis with HPLC, it was determined that the main phenolic compounds were caffeic acid phenethyl ester (CAPE), dimethoxycinnamic acid, caffeic acid, ferulic acid, p-coumaric acid, and vanillin. Propolis was extracted with cold-pressed virgin olive oil at different concentrations (control, 10, 20, 30 and 40%). The total phenolic content of these extracts was determined by Folin Ciocalteu, antioxidant activity by phosphomolybdenum, and antiradical activity by DPPH methods. It was determined that as the propolis concentration increased, total phenolic content, antioxidant, and antiradical activity also increased. The olive oil extract of propolis showed antipyretic effect in Wistar albino rats in which hyperthermia was induced by yeast. As a result, it is suggested that olive oil extract of propolis has antioxidant, antiradical and antipyretic effects; and it may be a healthier alternative to alcoholic extracts.

Innovative methods for the preparation of medical Cannabis oils with a high content of both cannabinoids and terpenes

Cannabis-based medications are being increasingly used for the treatment of different clinical conditions. Among all galenic formulations, olive oil extracts from medical Cannabis are the most prescribed ones for their easy preparation and usage. A great variety of methods have been described so far for the extraction of medical Cannabis oils to reach a high yield of Δ9-tetrahydrocannabinol (Δ9-THC), but poor attention has been paid to the preservation of the terpene fraction from the plant, which may contribute to the overall bioactivity of the extracts.In this context, the present study was aimed at the chemical characterization of different medical Cannabis oils prepared by following both innovative and existing extraction protocols, with particular attention to cannabinoids and terpenes, in order to set up a suitable method to obtain an extract rich in these chemical classes. In particular, six different extraction procedures were followed, based on different techniques, of which all but one included a decarboxylation of the plant material. The profile of cannabinoids was studied in detail by means of HPLC-ESI-MS/MS, while terpenes were characterized by means both GC-MS and GC-FID techniques coupled with solid-phase microextraction operated in the head-space mode (HS-SPME).An innovative method that is based on the extraction of the oil by dynamic maceration at room temperature from plant inflorescences, which were partially decarboxylated in a closed system at a moderate temperature and partially pre-extracted with ethanol, produced similar yields of bioactive compounds as that obtained by using a microwave-assisted distillation of the essential oil from the plant material, in combination with a maceration extraction of the oil from the residue. Both these new methods provided a higher efficiency over already existing extraction procedures of medical Cannabis oils and they can be applied to obtain a product with a high therapeutic value.

Potentiality of “orujillo” (olive oil solid waste) to produce hydrogen by means of pyrolysis

The extraction of olive oil generates great amounts of solid waste and wastewater. The objective of this work is to evaluate the potentiality of slow pyrolysis of the solid oil waste, known as “orujillo”, to produce hydrogen rich gases. The effect of temperature and of different treatments of pyrolysis vapors on the yield and composition of the gases has been experimentally studied. “Orujillo” was pyrolyzed at 500 °C and 700 °C, and the pyrolysis vapors produced were directly fed to a second reactor where they were treated at 800 °C in different ways: just thermally, thermally treated through a refractory alumina bed and thermo-catalytically treated through a three layer bed (alumina + SiC mixed with Ni catalyst + alumina). The catalyst used was a commercial prereduced nickel-catalyst which contains 44 wt% Ni over calcium aluminate support (CaO/Al2O3). Concerning the effect of temperature, it has been proved that the raise in temperature leads to a decrease in the liquid yields and an increase in the gas yields, as well as to an increase in H2 and a decrease in CO2 content of the gases. With respect to the vapors treatments it has been observed that as a general rule any of the treatments reduces liquid and increases gas yields, and concerning gas composition, H2 content increases, CO slightly increases and CO2 decreases. These effects are more pronounced when the Ni-catalyst is used. With the Ni-catalyst about 50 wt% gas yield is obtained, with ≈ 50 vol% H2 both at 500 and at 700 °C.

Comparative Effect of Hammer Mill Screen Size and Cell Wall-Degrading Enzymes During Olive Oil Extraction.

The influence of hammer mill screen size (4.5 and 8.5 mm) and enzyme addition (control and 500 ppm) on olive fruit cell wall breakdown and its consequences in terms of oil recovery and the phenolic content of olive oil was studied at the laboratory scale for “Arbequina” and “Koroneiki” at two different maturities. Water recovery and water-soluble carbohydrates in olive paste after malaxation were measured as an indicator of cell wall breakdown. Smaller screen size and enzymes increase oil recovery for Arbequina with a maturity index of 1.6 (6.3–6.6%); and for Koroneiki at a maturity index of 0.2 (15.0–38%) and 2.6 (1.3–4.3%). For both cultivars, the increase in oil recovery is larger in green fruits compared to more ripe fruit. Water recovery and water-soluble carbohydrates increase with small screen size and the enzyme treatments, even when no increment in oil recovery is observed. The water recovery range was 143–239% for Arbequina and 150–262% for Koroneiki; water-soluble carbohydrate range was 1.8–12.7 g/kg for Arbequina and 0.5–5.4 g/kg for Koroneiki. In general, smaller hammer mill screen size and enzymes increase total phenols in the oil, with a larger difference between control and treatment for green fruit than for the ripe fruit. For Arbequina, increases in total phenol content were in the range of 45–60 and 5–20% at maturity index 1.6 and 3.3, respectively. For Koroneiki, the increases were in the range of 31–121 and 7–9% at maturity index 0.2 and 2.6, respectively. Application of cell wall-degrading enzymes improves the cell wall breakdown caused by hammer mill, leading to higher oil recovery and total phenol content. The magnitude of the effect depends on the cultivar and olive fruit maturity.

On the Erosive Wear of Carbon Fiber-Reinforced Epoxy Composite in the Olive Oil Extraction Process

AbstractThe olive pomace erosion wear behavior of carbon fiber-reinforced epoxy composite (epoxy/CF) has been analyzed. A tribological test was carried out using an in house centrifugal testing setup in order to simulate the environment in olive oil extraction procedure. The environment is composed from a pomace-to-water ratio 70/30. The erosion wear-rate of this composite has been studied at different impact angles (45 deg and 90 deg) and at different impact velocities (200, 1200, 1500, and 2000 rpm). It was demonstrated that both the impact angle and the impact velocity have a significant effect on the carbon fiber-reinforced epoxy composite erosive wear-rate. The main findings reveal an erosive wear characterized by brittle damage; in fact, micro-cracks at the surfaces and sub-surfaces were found. The damage surfaces were marked by fragmented fibers.

Physicochemical Properties of Sponge Cake Fortified by Olive Stone Powder

Olive stones are by-products in olive oil extraction and in table olive industries. Nonetheless, they contain beneficial nutritive elements such as fiber, phenolic compounds, and omega-3 and -6 fatty acids. In this research, olive stone powder (OSP) was used to substitute 0, 15, 25, and 35% of wheat flour in sponge cake recipe. The effects of this substitution were examined on the physiochemical and sensory properties of sponge cakes. The fiber content and the total phenolic compounds of the cake samples increased significantly from 0.67 to 8.60% and from 89.14 to 739.23 μg/ml, respectively, by increasing the OSP from 0 to 35%. With increased amounts of OSP, batter viscosity and consistency decreased (from 27430 to 11030 centipoise and from 8.67 to 13.42 g/s, respectively), while the specific volume of the cakes increased (from 2.08 to 6.21 cm3/g). Parallel to the increased levels of substitution, the hardness and springiness of cakes decreased, whereas the gumminess and chewiness increased. The crumb structure of OSP-enriched cakes was more porous than that of the control. Color analysis indicated significant differences between the control and treated samples regarding L∗a∗, and b∗ parameters. Cakes had colors that were darker than expected when treated with OSP (p<0.05). According to sensory evaluations, no significant differences were observed between OSP cakes and the control sample in terms of taste, after taste, color, and odor (p>0.05). In conclusion, substituting OSP for flour by 25% can yield acceptable dietary fiber content and antioxidant phenolic compounds while showing no undesirable changes in sensory properties.

Utilization of olive pomace as a source of bioactive compounds in quality improving of toast bread

Olive oil pomace is produced as by-product with a large quantity during olive oil processing. It is a promising source for polyphenolic compounds and fibers which could be used in food industry. In this work proximate chemical analysis of olive pomace (two-phase olive oil extraction) was studied. Also, seven extracting solvents were tested in extracting the phenolic compounds from the olive pomace (OP). Total phenolic, flavonoids, and flavonols contents of the different extracts were determined. In addition to, the antioxidant activity of the phenolic extracts was investigated using 2, 2-diphenyl-1-picrihydrazyl (DPPH) to assess the efficiency of the extracting solvents. The obtained data rivaled that protein, fat, ash and fiber contents of OP were 2.48, 2.33, 1.33 and 20.37% (FW), respectively. It is clear that the OP contains a large quantity of fibers and it had cellulose content about 40.7% of the fiber content. Furthermore, the total phenolic content was varied in the different extracts and ranged from 8.29 to 36.24 mg GAE g -1. While, the total flavonoids were ranged from 2.23 to 12.52 mg QE g-1. Methanol and water (80:20) recorded the highest antioxidant activity with EC50 of 1.373g/g DPPH while, the acetone extract recorded the lowest antioxidant activity with EC50 of 8.052g /g DPPH. Toast bread was fortified with the cellulose isolated from OP at three replacement levels of 2, 4, and 6% and the results showed that there were no significant differences between control sample and the sample fortified with 2% cellulose in most of sensory characteristics tested. Addition of pomace cellulose at replacement level of 2% enhanced the texture of the bead and was more acceptable than the control. The results concluded that olive pomace is a good source for dietary fibers and polyphenolic compound which could be used in the food industry.

Change in some quality parameters and oxidative stability of olive oils with regard to ultrasound pretreatment, depitting and water addition

Ultrasound pretreatment with different times (0, 4, 8, 10 min) on olive paste previous malaxation of olive oil extraction along with depitting and water application procedures were studied. The effects of these procedures on oil yield, quality parameters and oxidative stability have been studied. Therefore, the olive oil yield increase with increasing time of ultrasound treatment. On the other hand, the application of ultrasound did not adversely affect the quality characteristics, as well as the antioxidant activity, when comparing with untreated paste. Furthermore, the oxidative stability data, we can conclude that ultrasound treatment can affect the olive oil oxidative stability. This study could provide useful information for industry to produce olive oil with high yield and quality.

By-Products of Olive Oil in the Service of the Deficiency of Food Antioxidants: The Case of Butter

Further downstream in the olive oil extraction process, the Mediterranean Basin faces a serious environmental threat caused by olive waste. Despite their polluting profile, olive waste is considered to be a very rich source of natural antioxidants, such as polyphenols. In this study, the latter was valued as a source of natural antioxidants and compared with a synthetic antioxidant ascorbic acid. Concentrations of 2, 4, 6, and 8 mg of the olive mill waste water as well as pomace and ascorbic acid are added to butter (commercial butter) and placed under storage conditions in the oven (accelerated test: 60°C) for 3 months. The alteration of the butter used was followed by determination of the peroxide value and acidity and microbiological analysis. The results obtained show that butters containing olive by-products have undergone less marked oxidative deterioration than those of the control (without additives). The best oxidative stability of butter was achieved by adding 80 mg/kg of butter, a result comparable with that obtained by adding ascorbic acid.

Fourier transform near infrared spectroscopy as a tool to discriminate olive wastes: The case of monocultivar pomaces

Olive pomace, a wet semi-solid paste that remains after olive oil extraction, is a major waste of the process and its recovery is mandatory due to its phytotoxicity when rejected directly into the soil. Innovative applications have been studied, but simple and reliable methods that fulfil the gap between the recovery of compounds and their use by industries (contributing to the sustainability and circular economy of the chain) still need to be explored.In this work, four monocultivar olive pomaces (Arbequina, Arbosana, Oliana, and Koroneiki) were studied regarding their nutritional composition, fatty acids and vitamin E profiles, total phenolic and flavonoid contents, antioxidant activity, and Fourier Transform Near Infrared and Mid Infrared spectra. Principal Component Analysis and Partial Least Square Discriminant Analysis were used to discriminate samples.Arbosana pomace presented the highest total fat (15%, dw) and vitamin E contents (1.4 mg/g of oil), being α-tocopherol the main vitamer in all samples. Koroneiki pomace was the richest in phenolic compounds (9 g gallic acid eq./kg). The major fatty acid was oleic acid. Stearic acid, linoleic acid, and FRAP levels differed significantly among cultivars. NIR spectra showed differences in all spectral regions (best separation from 6504 to 5389 cm−1 and 4961 to 4035 cm−1), while MIR spectra presented differences only in some spectral regions.The results showed that Near Infrared spectroscopy together with Principal Component Analysis is a powerful tool to discriminate olive pomace cultivars, with ability to be used in an industrial context.

Contribution to improvement of the traditional extraction of olive oil by pressure from whole and stoned olives by addition of a co-adjuvant (talc)

The olive growing is one of the strategic sectors of the Algerian economy. Traditional olive culture located in the mountains of Kabylia offers typical oil widely preferred by a large part of the Algerian consumers. However, this ancestral culture risks not only abandonment but suffers much more from uncontrolled of the good practices surrounding this local product requiring a valorization accompanied by improvement. Indeed, the difficulty to extract the total oil contained in the fruit is one of the main obstacles of the extraction method particularly by pressure. Nevertheless, some actions as the addition of co-adjuvant during the malaxing process allow improving efficiency of the extraction process. Our results indicate that the addition of 2.5% of talc as a co-adjuvant to a moistless paste obtained from whole olives significantly improves the oil yield by about 4.4% without altering the acidity compared to the control. Similarly, stoning improves the moisture of the pressed mass although without improving yield of the oil characterized by a slight decrease in acidity compared to other extracted oils. Therefore, our results confirm the beneficial effect of talc on the extraction of olive oil and contribute to the improvement of the traditional extraction by pressure to enhance the value of this local product.

Fluid dynamic-based Engineering design of a Full-Scale Device for the improvement of Extra Virgin Olive Oil Yield and Quality by means of Combined Acoustic Cavitation and Thermal Conditioning

After some hesitations, the scientific community is jointly converging on the benefits due to the ultrasound treatment by means of mechanical effects generated by acoustic cavitation phenomena occurring into the olive oil paste proposed initially by Amirante and Clodoveo. In recent works, many authors have now confirmed that this promising emerging technology produces relevant beneficial effects if applied to the extraction process under well-controlled conditions. In the last years, the industrial applications of ultrasound (US) in the Extra-Virgin Olive Oil (EVOO) extraction process are changing the paradigm of the knowledge in this field of interest due to a great effort of the research activity. In the present work, the design of the device by means a Three-Dimensional (3D) Multiphase Computational Fluid Dynamic (CFD) analysis was performed, which describes the ultrasound effects in the olive paste, necessary to control the US waves propagation. Thus, fluid dynamic analysis allowed to predict the flow path in the ultrasound devices, to evaluate the flow parameters of the olive paste inside the SHE and the cavitation phenomenon, with the aim to find an optimal design, capable to ensure the best ultrasounds and mixing effects. Moreover, experimental results demonstrated that the machine can guarantee an actual simultaneous improvement of the olive oil extraction yield, as well as of the product quality. Finally, the results from sensory evaluations are summarized confirming the goodness of EVOO obtained by means of US.

Consuming nutritional doses of olive pomace oil fulfils recommended dietary allowances of alpha-tocopherol. Results from a randomized clinical trial

AbstractVitamin E is a powerful antioxidant that prevents free radical propagation in membranes and plasma lipoproteins. Recommended Daily Allowance (RDA), stablished at 13 and 11 mg/day for adult men and women, respectively, is not fulfilled in most European countries. Thus, strategies to cover the daily recommendations of this vitamin are essential, and one may be to encourage the consumption of foods rich in this micronutrient.Olive-pomace oil (OPO), which is obtained from the solid by-product obtained during olive oil extraction, is nutritionally relevant due to its high content in oleic acid (C18:1), among other monounsaturated fatty acids. In addition, OPO contains a wide range of minor bioactive components that may contribute to its healthy properties. Among these, it is especially relevant the high content of vitamin E in OPO.The objective of the present study was to assess the effect of consuming nutritional relevant doses of olive pomace oil within a normal, non-supplemented diet, in the vitamin E status in adults.We carried out a randomized, cross-over, controlled clinical trial, 14 weeks long in 70 subjects, consisting in two 4-week interventions in which volunteers consumed either OPO or high oleic sunflower oil (HOSO) preceded each by 3-week wash-out steps with sunflower oil. Participants daily consumed 50 mL of either OPO or HOSO, containing 357 and 420 ppm vitamin E, respectively, to meet the nutritional requirements of fat intake. Intake of macronutrients and micronutrients (vitamin E) was analyzed using the DIAL software (Faculty of Pharmacy, Complutense University of Madrid).The results obtained showed that the average consumption of vitamin E increased from 9.61 mg/day (intake of volunteers before starting the study) to 17.76 mg/day and 18.45 mg/day after the intervention with OPO and HOSO, respectively. Acceptance tests to evaluate the level of satisfaction of both oils showed higher preference of OPO compared to HOSO.OPO was well accepted and, thus, constitutes an important dietary source of vitamin E covering the recommended daily intake. It is worth thinking about OPO as part of a healthy diet.

Textile Dye removal by Adsorption on Olive Grain as Solid Waste from the Olive Oil Extraction

Textile Dye removal by Adsorption on Olive Grain as Solid Waste from the Olive Oil Extraction

OLIVE MILL WASTEWATER (OMW) TREATMENT BY HYBRID PROCESSES OF ELECTROCOAGULATION/CATALYTIC OZONATION AND BIODEGRADATION

The olive oil extraction industry is one of the most polluting wastewaters from the food industry, which is considered an important economic activity in the world. The environmental effects of olive wastewater are related to chemical and organic loading, which is resistant to biodegradation processes. The purpose of this study was to determine the efficiency of combined electrocoagulation, catalytic ozonation, to reduce organic load and improve the biodegradability of olive oil sewage. The present study was experimental on a laboratory scale and was done at a batch reactor on actual olive wastewater. The electrocoagulation process was performed using Fe electrodes at a current density of 0.73, 0.5, 0.05..dm... The optimal conditions for the electrocoagulation process were obtained at a flow rate of 0.5 ..dm. for 45 min reaction time, so that the removal efficiency of TOC, COD, and turbidity was 75, 80 and 94%, respectively. Then the treated wastewater under these optimal conditions entered the catalytic ozonation process (COP) reactor. In the COP process, the TOC and COD concentrations reached 105 and 210 mg/L after 90 min (44% and 56% removal). Eventually, the effluent was introduced into the biology*ical reactor and the removal efficiency of COD and TOC were investigated. So, the amount of TOC and COD decreased from 105 and 210 mg/L to 22.3 mg/L and 37 mg/L, respectively. As a result, the overall efficiency of the EC/COP/SBR system in removing COD and TOC was 98.4% and 97.2%, respectively. The combination of these processes is very effective in comparison with other processes for the treatment of olive sewage.

Pulsed Electric Fields for the Treatment of Olive Pastes in the Oil Extraction Process

The aim of this study was to evaluate the ability of pulsed electric field (PEF) technology to improve the extractability and enhance the oil quality in an industrial olive oil extraction process. Using a PEF device on olive pastes significantly increased the extractability from 79.5% for the control, up to 85.5%. The PEF system did not modify the primary legal quality parameters or total concentrations of phenols, aldehydes, and esters. On the contrary, the non-thermal treatment slightly enhanced the dialdehydic forms of decarboxymethyl elenolic acid linked to hydroxytyrosol (3,4-DHPEA-EDA) and tyrosol (p-HPEA-EDA), and decreased the total saturated and unsaturated C5 and C6 alcohols of the PEF EVOO (Extra Virgin Olive Oil) compared to the control test. This study confirmed that PEF technology can improve olive oil extraction and quality.

Antioxidant, Antibacterial and Antifungal Effects of Phenolic Extracts of Extra Virgin Olive Oil from Two Western Regions of Algeria: A Comparative Study

The aim of the study was to evaluate the antioxidant, antibacterial and antifungal activities of phenolic extracts of extra virgin olive oil obtained from two distinct regions in Northwest Algeria. The first extra virgin oil (CHIALI) was produced industrially according to the international standards, whereas the second (TRAD) was produced by traditional methods. Antioxidant activity was evaluated using the DPPH (diphenylpicrylhydrazine) method. The antimicrobial activity of the two phenolic extracts was assessed against Escherichia coli (ATCC25922), Pseudomonas aeruginosa (ATCC27853), Staphylococcus aureus (ATCC25923), Alternaria sp., Candida albicans by using microdilution and disk diffusion methods. The extraction yield was found to be 26.7% and 20.5% for the industrial (CHIALI) and traditional methods (TRAD), respectively. The phenolic extract of the industrial extra virgin oil exhibited better antioxidant activity (IC50 = 1.56 μg/ml) when compared to those of the traditional oil (IC50 = 6.27 μg/ml). Both phenolic extracts exerted bactericidal and antifungal activities. These activities were more important with the phenolic extract of the industrial oil. Taken together, our results showed that the phenolic extract of the industrial extra virgin oil had better antioxidant, antibacterial and antifungal activities, owing to the extraction methods used.