Olive Leaves Research Articles

Response of laying hens fed diet supplemented with a mixture of olive, laurel, and rosemary leaf powders: Metabolic profile, oxidative status, intestinal histomorphology, and egg quality

This study aimed to evaluate the effects of a mixture of olive, laurel, and rosemary leaf powders, on the oxidative state, biochemical, immune, intestinal morphophysiological parameters, and egg quality of laying hens. One hundred Lohmann Brown hens (28 weeks old) were equally assigned to two groups (n. 50) corresponding to a basal control diet (CON) or the diet supplemented with 6 g/kg feed of leaf powder mixture (LPM) containing olive, laurel, and rosemary leaves (1:1:1), for 60 days. Oxidative status, biochemical indices, immune response, cecal short chain fatty acids (SCFAs), intestinal morphological characteristics, and some egg traits were evaluated at the end of the experiment. The results indicated that LPM improved (P < 0.05) the oxidative status (TOS, ROMs), the immune system (IL-6, IL-1β, and TNF-α), the total protein and HDL cholesterol content, whereas it decreased (P < 0.05) total cholesterol and LDL cholesterol. Aspartate aminotransferase (AST), alkaline phosphatase (ALP), and alanine aminotransferase were significantly (P < 0.05) lower in the LPM than in the CON group. A significant increase (P < 0.05) in SCFA content in the caecum, as well as in villi height and crypt depth in both duodenum and ileum of LPM-treated hens, was observed. Egg quality parameters were not influenced (P > 0.05) by LPM. These findings indicate that LPM can be considered a candidate as an antioxidant ingredient for functional food in laying hens.

Olive leaf extract-derived chitosan-metal nanocomposite: Green synthesis and dual antimicrobial-anticancer action

In this study, we developed a novel nanocomposite by synthesizing zinc (ZnNPs), copper (CuNPs), and silver (AgNPs) nanoparticles using olive leaf extract and incorporating them into a chitosan polymer. This approach combines the biocompatibility of chitosan with the antimicrobial and anticancer properties of metal nanoparticles, enhanced by the phytochemical richness of olive leaf extract. The significance of our research lies in its potential to offer a biodegradable and stable alternative to conventional antibiotics and cancer treatments, particularly in combating multidrug-resistant bacteria and various cancer types. Comprehensive characterization through Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction (XRD), Thermogravimetric Analysis (TGA), Scanning Electron Microscopy (SEM), Energy Dispersive X-ray (EDX), and Transmission Electron Microscopy (TEM) confirmed the successful synthesis of the nanocomposites, with an average size of ~22.6 nm. Phytochemical analysis highlighted the antioxidant-rich composition of both the olive leaf extract and the nanoparticles themselves. Functionally, the synthesized nanoparticles exhibited potent antimicrobial activity against multidrug-resistant bacterial strains, outperforming traditional antibiotics by inhibiting key resistance genes (ermC, tetX3-q, blaZ, and Ery-msrA). In anticancer assessments, the nanoparticles showed selective cytotoxicity towards cancer cells in a concentration-dependent manner, with CuNPs and AgNPs showing particularly strong anticancer effects, while demonstrating minimal toxicity towards normal cells. ZnNPs were noted for their low cytotoxicity, highlighting the safety profile of these nanoparticles. Further, the nanoparticles induced apoptosis in cancer cells, as evidenced by the modulation of oncogenes (P21, P53, and BCL2), suggesting their therapeutic potential. The findings of our study underscore the versatile applications of these biogenic nanoparticles in developing safer and more effective antimicrobial and anticancer therapies.

Green synthesis of NiFe2O4, CoFe2O4, and Ni0.5Co0.5Fe2O4 by sol–gel autocombustion method using olive leaf extract as fuel

Green synthesis of NiFe2O4, CoFe2O4, and Ni0.5Co0.5Fe2O4 by sol–gel autocombustion method using olive leaf extract as fuel

Effects of olive leaf extract and nifedipine, alone and in combination, on blood pressure, neutrophil gelatinase-associated lipocalin, malondialdehyde, and creatinine levels in an Nω-nitro-L-arginine methyl ester-induced rat model of preeclampsia

Preeclampsia (PE) is a significant health problem in pregnancy, affecting 6–7% of all gestations and leading to fetal growth retardation, infant morbidity and mortality, premature birth, and maternal death. Currently, effective treatment options for PE are limited. This study aimed to evaluate the therapeutic potential of olive leaf extract (OLE) and nifedipine, alone and in combination, in improving pregnancy outcomes in rats induced with Nω-nitro-L-arginine methyl ester hydrochloride (L-NAME). Rats were treated with single and combination treatments of nifedipine and OLE for four weeks, after being induced with L-NAME for six weeks. Following gestational day assessment and systolic blood pressure testing, the rats were sacrificed, and blood samples were collected for Neutrophil Gelatinase-Associated Lipocalin (NGAL), Malondialdehyde (MDA), proteinuria and Creatinine (SCr) Levels examinations. The results revealed the combination of nifedipine and OLE significantly improved the results of several biomarker analyses associated with PE, including increased NGAL levels, a significant decrease in MDA levels, decrease in proteinuria and elevated creatinine levels. The combination nifedipine and OLE treatment also exhibited a significant antihypertensive effect compared to either nifedipine or OLE monotherapy, as evidenced by the reduction in systolic and diastolic blood pressure levels. This study provides novel evidence for the potential of olive leaf extract as a nutraceutical adjunct for the prevention and treatment of PE. The significant improvements observed in several pathological features associated with PE, including antihypertensive effects, warrant further investigation. Future research should focus on elucidating the underlying mechanisms of action and exploring the clinical applicability of olive leaf extract in human populations with PE.

Development of Coated PLA Films Containing a Commercial Olive Leaf Extract for the Food Packaging Sector.

A commercial olive leaf extract (OL), effective against Salmonella enterica, Escherichia coli, Listeria monocytogenes, and Staphylococcus aureus, was added to three different coating formulations (methylcellulose, MC; chitosan, CT; and alginate, ALG) to produce active polylactic acid (PLA) coated films. Evaluation of these coated PLA films revealed significant inhibition of S. aureus growth, particularly with the MC and CT formulations exhibiting the highest inhibition rates (99.7%). The coated films were then tested for food contact compatibility with three food simulants (A: 10% ethanol; B: 3% acetic acid; D2: olive oil), selected to assess their suitability for pre-cut hams and ready-to-eat vegetables in relation to overall migration. However, coated films with active functions exhibited migration values in simulants A and B above legal limits, while promising results were obtained for simulant D2, highlighting the need to deeply investigate these coatings' impact on a real food system. Untargeted metabolomics revealed that the type of coating influenced the selective release of certain phenolic classes based on the food simulant tested. The Oxitest analysis of simulant D2 demonstrated that the MC and ALG-coated PLA films slightly slowed down the oxidation of this food simulant, which is an edible vegetable oil.

In vitro biological evaluation and in silico insights into the antiviral activity of standardized olive leaves extract against SARS-CoV-2.

There is still a great global need for efficient treatments for the management of SARS-CoV-2 illness notwithstanding the availability and efficacy of COVID-19 vaccinations. Olive leaf is an herbal remedy with a potential antiviral activity that could improve the recovery of COVID-19 patients. In this work, the olive leaves major metabolites were screened in silico for their activity against SARS-CoV-2 by molecular docking on several viral targets such as methyl transferase, helicase, Plpro, Mpro, and RdRp. The results of in silico docking study showed that olive leaves phytoconstituents exhibited strong potential antiviral activity against SARS-CoV-2 selected targets. Verbacoside demonstrated a strong inhibition against methyl transferase, helicase, Plpro, Mpro, and RdRp (docking scores = -17.2, -20, -18.2, -19.8, and -21.7 kcal/mol.) respectively. Oleuropein inhibited 5rmm, Mpro, and RdRp (docking scores = -15, -16.6 and -18.6 kcal/mol., respectively) respectively. Apigenin-7-O-glucoside exhibited activity against methyl transferase and RdRp (docking score = -16.1 and -19.4 kcal/mol., respectively) while Luteolin-7-O-glucoside inhibited Plpro and RdRp (docking score = -15.2 and -20 kcal/mol., respectively). The in vitro antiviral assay was carried out on standardized olive leaf extract (SOLE) containing 20% oleuropein and IC50 was calculated. The results revealed that 20% SOLE demonstrated a moderate antiviral activity against SARS-CoV-2 with IC50 of 118.3 μg /mL. Accordingly, olive leaf could be a potential herbal therapy against SARS-CoV-2 but more in vivo and clinical investigations are recommended.

Valorization of edible films based on chitosan/hydroxyethyl cellulose/olive leaf extract and TiO2-NPs for preserving sour cream

Biodegradable edible films for sour cream packaging were developed based on chitosan (CS), hydroxyethyl cellulose (HEC), Olive leaf extract (OE), and titanium dioxide nanoparticles (TiO2-NPs). The prepared CS/HEC/TiO2-OE bionanocomposite films were evaluated for their antimicrobial and antioxidant activities as well as using FT-IR, mechanical, permeability, and contact angle. The effect of developed films on the lipid oxidation, microbiological load, and chemical properties of sour cream was investigated. The fabricated films had an antimicrobial impact against all tested strains. The film containing 8 % OE showed effective protection against fat oxidation, with a peroxide value of 3.21 meq O2/kg, a para-anisidine value 5.40, and free fatty acids of 0.82 mg KOH/kg. The films with OE 4 % and 8 % have a good effect on the microbiological load of sour cream for 90 days. These films did not influence the chemical composition of sour cream and therefore can be used in this sort of dairy product.

Comparative Analysis of Olive-Derived Phenolic Compounds' Pro-Melanogenesis Effects on B16F10 Cells and Epidermal Human Melanocytes.

Olive leaf contains plenty of phenolic compounds, among which oleuropein (OP) is the main component and belongs to the group of secoiridoids. Additionally, phenolic compounds such as oleocanthal (OL) and oleacein (OC), which share a structural similarity with OP and two aldehyde groups, are also present in olive leaves. These compounds have been studied for several health benefits, such as anti-cancer and antioxidant effects. However, their impact on the skin remains unknown. Therefore, this study aims to compare the effects of these three compounds on melanogenesis using B16F10 cells and human epidermal cells. Thousands of gene expressions were measured by global gene expression profiling with B16F10 cells. We found that glutaraldehyde compounds derived from olive leaves have a potential effect on the activation of the melanogenesis pathway and inducing differentiation in B16F10 cells. Accordingly, the pro-melanogenesis effect was investigated by means of melanin quantification, mRNA, and protein expression using human epidermal melanocytes (HEM). This study suggests that secoiridoid and its derivates have an impact on skin protection by promoting melanin production in both human and mouse cell lines.

Impact of olive leaves powder addition on extra virgin olive oil: Sensory, quality, nutritional and volatile compounds implications

AbstractOlive leaves currently represent a waste from olive oil industry that can be reused as source of polyphenols and other compounds. The objective of this study was to test whether incorporation of olive leaf powder directly in olive oils can enhance and modify its chemical‐sensory quality. Thus, leaves from cultivar “Koroneiki” were washed, sanitized, dried (37–40°C for 48 h) and milled until obtaining a powder that was added to “Arbequina” and “Koroneiki” extra virgin olive oils, at 1% and 3%. The oils were stored in dark conditions at room temperature and samples were checked after 0, 3, 6 and 12 months. The quality parameters, sensory properties, and nutrition quality (total phenolics, antioxidant, oleuropein and alpha‐tocopherol) were evaluated. The olive leaves affected quality and chemical composition, mainly increasing resistance to oxidation, which was not verified in samples without leaves addition. The incorporation of leaves powder significantly increased the contents of C6‐C5 alcohols/aldehydes, intensity of the green fruity and bitter, also artichoke, herbs, tomato leaf, olive leaves and banana peels sensations.

Antibacterial Activity of Prepared Zinc Oxide Nanoparticles by Eco-Friendly Method Using Olive Leaves and Roselle Flower Extracts

This research looked at the antimicrobial properties of zinc oxide nanoparticles (ZnO NPs) made from roselle flower extracts and olive leaves as part of a green synthesis method. We used field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), ultraviolet-visible (UV–vis) spectroscopy and Fourier-transform infrared (FTIR) spectroscopy to study the morphological, structure and optical properties of ZnO NPs. The Field Emission Scanning Electron Microscopy (FESEM) test revealed that the ZnO NPs (Olive leaves) had round particles with sizes ranging from 29.03[Formula: see text]nm to 53.59[Formula: see text]nm, while the ZnO NPs (Roselle flower) had nanoflakes with sizes ranging from 42[Formula: see text]nm to 80[Formula: see text]nm. By using UV–vis spectroscopy, it was found that the optical energy gap for ZnO NPs derived from olive leaves was about 2[Formula: see text]eV and for ZnO NPs derived from rose flowers it was 2.2[Formula: see text]eV. The antimicrobial properties of ZnO nanoparticles (NPs) were assessed against two types of bacteria: gram-negative bacteria (Klebsiella pneumoniae) and gram-positive bacteria Staphylococcus aureus (S. aureus). Both plant extracts exhibited a maximal inhibitory zone of 21[Formula: see text]mm against gram-positive bacteria, specifically S. aureus.

Protective effect of olive leaf (Olea europaea L.) extract against chronic exposure of liver and kidney tissues of Wistar rats to aluminum chloride

Introduction: The liver and kidney are the main sites of aluminum (Al) accumulation. Lifetime exposure to significant amounts of Al is inevitable, hence its toxicity on the liver and kidney should be a health concern. Natural antioxidants have been proven to alleviate pathologies in various liver and kidney injuries. However, the effect of olive leaf extract (OLE) on Al-exposed animals is yet to be confirmed. This study aimed to investigate the OLE effect against AlCl3 chronic exposure in rats’ liver and kidneys. Methods:Thirty-two male Wistar rats were divided into four groups (n=8), including the control group, the AlCl3 group treated with 128 mg/kg AlCl3 solution, as well as AlCl3 +OLE50, and AlCl3 +OLE100 groups (Other than AlCl3 they received 50 and 100 mg/kg of OLE, respectively, 2 hours after AlCl3 administration). All treatments were given orally for 12 weeks. All groups were evaluated for liver and kidney histopathological features, then scoring was performed. Results: AlCl3 administrations produced histopathological lesions in the liver and kidney, indicated by increased liver necro-inflammatory grades, ballooning scores, and renal inflammatory cell infiltration (P&lt;0.05). OLE100 mg/kg significantly reduced liver necroinflammatory grade, ballooning score, and kidney inflammatory cell infiltrations. The dose of 50 mg/kg also reduced these parameters (P&lt;0.05), except for the liver necro-inflammatory grade. There was a significant correlation between OLE dose and liver necro-inflammatory grade and ballooning score amelioration. Conclusion: OLE ameliorates liver and kidney histopathological features induced by oral Al chronic exposure in a dose-dependent manner.

Olive leaf extract green-formulated manganese nanoparticles: Characterization and antioxidant, cytotoxicity and anti-breast carcinoma properties

Olives are rich in many plant compounds; Antioxidants in olives protect against the occurrence of many cardiovascular diseases, blood pressure, diabetes, various types of cancers and tissue damage caused by free radicals. These antioxidant compounds include oluropine, hydroxytyrosol, tyrosol, oleanolic acid, and quercetin. Recent studies have focused on the therapeutic efficacies of manganese nanoparticles mediated by medicinal plants. The study investigated the characteristics of green manganese nanoparticles formulated using olive leaf extract on breast carcinoma cells. The nanoparticles were analyzed using FE-SEM, XRD, FT-IR, and UV–Vis techniques. The formation of manganese nanoparticles was confirmed by observing bands at 219 and 290 nm. The Mn-O vibration can be identified by the peaks observed at 516 and 610 cm−1. The peaks at 72.69, 60, 53.26, 44.97, 40.25, 34.83, and 28.48 corresponding to manganese nanoparticles (444), (440), (510), (420), (400), (222), and (220) diffraction planes, indicate the manganese nanoparticles formation. The MTT assay was employed to evaluate the anti-breast carcinoma effects (on CAMA-1 and MCF-7 cells) as well as the cytotoxicity effects (on HUVEC cell line) of manganese NPs. Results from the DPPH test showed that MnNPs and BHT at doses of 231 and 84 μg/ml were able to eliminate 50 % of radicals. The findings from the MTT experiment indicate that over 3 days, the cancer cells percentage that survived decreased proportionally to the increase in nanoparticle concentration. Furthermore, the data demonstrate that the cancer cells survival rate decreased with the rise in nanoparticle concentration, ultimately reaching a point where no viable cells were observed at a concentration of 1000 μg/ml. The most effective cytotoxicity was observed at a concentration of 1000 μg/ml. The MTT findings indicate that the IC50 values are 173 and 256 µg/ml, representing the concentration of nanoparticles at which half of the CAMA-1 and MCF-7 breast carcinoma cells in the culture medium are eradicated. Analysis of the gene expression levels of PIK3, AKT, and mTOR, linked to the mTOR pathway, in addition to Bax, Bcl2, and Caspase-3, demonstrates that copper nanoparticles efficiently control PC14 cell apoptosis and proliferation by regulating the PI3K-Akt-mTOR signaling pathway. The mTOR pathway could potentially be related to the suppression of the cell cycle and the initiation of apoptosis due to nanoparticles.

Extraction and Characterization of Natural Antioxidants from Olive Leaves Powder

Olive (Olea europaea L.) leaves, which contain large amounts of phenols, are a common byproduct in the production of olive oil. When improperly processed, the by-products produced by the olive diligence can impairment the environment. Its leaves, which are made when olive trees are pruned and harvested and it is expected that foliage make up twenty five percent of pruning remains overall. These byproducts cost manufacturers money and have serious environmental implications. So, like other agronomical production wastes, partial reuse is something that should be pursued. These leftovers have a high concentration of beneficial chemicals, if isolated, may be used in food, cosmetics and nutraceutical sectors. Objectives: To extract the bioactive compounds polyphenols and flavonoids in 70% ethanol extract and its antioxidant activity was done. Methods: The polyphenols were quantified by Folin reagent and flavonoids by aluminum chloride methods respectively and the natural antioxidants were estimated by using free radical scavenging DPPH assay. Results: It was discovered that the 70% ethanol extract's total polyphenolic content was 65.50 ± 1.42 (mg GAE/g) and its total flavonoids were 11.85 ± 0.60 (mg QE/g). In 70% ethanol extract the % inhibition (DPPH) was 42.82±3.20-88.40±5.18 while BHT has the % inhibition (DPPH) 30.4 ± 2.50-80.50 ± 4.68 at concentration 0.1-0.5 mg/ml. Conclusions: The results indicating a noteworthy antioxidant activity in terms of radical scavenging activity. These results also wrapped up that the olive industry waste may be reutilized as a natural source of antioxidants in various sectors.

Image Analysis and Detection of Olive Leaf Diseases Using Recurrent Neural Networks

The widespread adoption of DL has led to a rise in academic interest in image recognition approaches, enabling applications such as automated image classification and the detection of plant diseases. The world's largest producer of olives is Morocco. Plant health might be harmed by illnesses, which therefore affects its development. Numerous illnesses affecting olive leaves specifically target crop growth rate. The objective of this research is to create deep RNNs to identify olive plant illnesses using a collection of leaf images, collected from various sources (Disease note The peacock eye falls on olive trees, Field Guide to Olive Pests, Diseases and Disorders in Australia. Thus, this technique is the best RNN model and is employed in further applications to enhance diagnostic measurements regarding olive leaves and other plant leaves.

Biocontrol efficacy of native protease-producing bacterial isolates against the olive leaf moth, Palpita unionalis (Hübner) (Lepidoptera: Pyralidae)

BackgroundThe olive leaf moth (OLM), Palpita unionalis (Hübner) (Lepidoptera: Pyralidae), poses a significant threat to olive cultivation. This study focused on isolating protease-producing bacteria from moribund field strains of P. unionalis larvae and assessing their larvicidal efficacy against laboratory strains through estimated LC30, LC50, and LC90 values. Additionally, the long-lasting impacts of LC50 values on pupal and adult stages were evaluated.ResultsThree entomopathogenic bacteria, Bacillus amyloliquefaciens, Pseudomonas putida, and Bacillus subtilis, were isolated. The larvicidal efficacies of these isolates varied, as B. subtilis exhibiting greater efficacy at LC30 and LC50 values, while P. putida showed the most potent effect at the LC90 value. Conversely, B. amyloliquefaciens demonstrated the lowest larvicidal potentialities at LC50 and LC90 values, with P. putida being the least effective at the LC30 value. The toxicity regression lines indicated increased larval lethality with each unit increase in concentration, as evidenced by the positive slope values. Chi-square (χ2) values suggested a better fit and supported the reliability of the estimated lethal concentrations. Exposure to LC50 values s resulted in significant elongation of larval duration and reduction in weight, whereas pupal lifespan, adult emergence, and sex ratio showed non-significant differences compared to untreated controls.ConclusionsThe study highlights the promising role of these biological candidates in pest control programs as eco-friendly alternatives, potentially enhancing safe plant production. The varied larvicidal efficacies of the bacterial isolates, along with their proteolytic capabilities, underscore their potential as biocontrol pathogens against OLM, contributing to sustainable agricultural practices.

Exploring the Nutritional and Bioactive Potential of Olive Leaf Residues: A Focus on Minerals and Polyphenols in the Context of Spain's Olive Oil Production.

Lyophilized plant-origin extracts are rich in highly potent antioxidant polyphenols. In order to incorporate them into food products, it is necessary to protect these phytochemicals from atmospheric factors such as heat, light, moisture, or pH, and to enhance their bioavailability due to their low solubility. To address these challenges, recent studies have focused on the development of encapsulation techniques for antioxidant compounds within polymeric structures. In this study, lyophilized olive leaf extracts were microencapsulated with the aim of overcoming the aforementioned challenges. The method used for the preparation of the studied microparticles involves external ionic gelation carried out within a water-oil (W/O) emulsion at room temperature. HPLC analysis demonstrates a high content of polyphenols, with 90% of the bioactive compounds encapsulated. Meanwhile, quantification by inductively coupled plasma optical emission spectroscopy (ICP-OES) reveals that the dried leaves, lyophilized extract, and microencapsulated form contain satisfactory levels of macro- and micro-minerals (calcium, potassium, sodium). The microencapsulation technique could be a novel strategy to harness the polyphenols and minerals of olive leaves, thus enriching food products and leveraging the antioxidant properties of the polyphenolic compounds found in the lyophilized extract.

Acceptance of a new product based on yerba mate and olive leaf

The olive industry is constantly growing in the State of Rio Grande do Sul, Brazil. It generates a number of waste products, including the Olea europaea L leaf, commonly known as olive leaf. This work presents an invention composed of yerba mate (Ilex paraguariensis ST Hil.) and Olea europaea L leaves, two raw materials capable of offering benefits to human health, according to the literature. The objective was to develop a new product from the combination of yerba mate and leaves of Olea europaea L. Sensory analysis tests were carried out with 100 mate consumer evaluators, in order to investigate the acceptability of the product, the purchase intention, and sensations when tasting the product. Furthermore, a test was carried out to find out the consumer's perception of an ideal mate, through the CATA (check-all-that-apply) test. The product achieved 82% acceptability and 82.4% purchase intention. The sensations most noted by the evaluators during the sensory analysis were good appearance, pleasant flavor, and watery flavor. The incorporation of Olea europaea L leaves into yerba mate can create a new product that can be placed on the market.

Chemical and structural heterogeneity of olive leaves and their trichomes

Many biological surfaces have hairs, known as trichomes in plants. Here, the wettability and macro- and micro-scale features of olive leaves are analyzed. The upper leaf side has few trichomes, while the lower side has a high trichome density. By combining different techniques including electron and atomic force microscopy, trichome surfaces are found to be chemically (hydrophilic-hydrophobic) heterogeneous at the nano-scale. Both olive leaf surfaces are wettable by water, having a high water contact angle hysteresis and great drop adhesion. The ultra-structural pattern observed for epidermal pavement cells differs from the reticulate cuticle structure of trichomes which shows that leaf surface areas may be substantially different despite being located nearby. Our study provides evidence for the nano-scale chemical heterogeneity of a trichome which may influence the functional properties of biological surfaces, such as water and solute permeability or water capture as discussed here for plants.

Enhancement of Soybean Oil Antioxidant Activity by Enrichment with Olive Leaves Extracts from Tunisian “Jéli and Octobry” Varieties

Enhancement of Soybean Oil Antioxidant Activity by Enrichment with Olive Leaves Extracts from Tunisian “Jéli and Octobry” Varieties

Olive Leaves and Citrus Peels: From Waste to Potential Resource for Cosmetic Products

Resource depletion and food waste accumulation represent a tremendous socio-economic and environmental problem. One promising strategy involves the use of byproducts derived from food waste as ingredients for cosmetic products. The aim of this work is to propose clementine peels and olive leaf extracts as value-added bioproducts for a cosmetic cream. Extracts were obtained by super critical extraction showing an antioxidant activity of ca. 25%. No cytotoxic effects of the extracts were recorded on keratinocyte cells up to a concentration of 4% v/v ratio within 24 h. The incorporation of clementine peels and olive leaf extracts into creams did not compromise their stability, as demonstrated by Turbiscan analyses at room and extreme (40 °C) storage conditions. The safety profiles of the final cosmetic formulations were further in vivo demonstrated on human volunteers. We analyzed the trans-epidermal water loss and variation of the skin’s erythematous index, which showed profiles that almost overlapped with the negative control. Moreover, rheological analysis of the resulting creams evidences their suitable spreadability with similar pseudoplastic profiles, although a slight reduction of viscosity was recorded by improving the extracts’ concentrations. The proposed approach highlights the advantage of combining byproduct resources and supercritical fluid extraction to obtain a safe and eco-friendly face cream.