Oleifera Seed Extract Research Articles

The impact of Moringa oleifera on the health status of Nile tilapia exposed to aflatoxicosis

The present investigation evaluated the negative impacts of an aflatoxin-contaminated diet on the growth performance, serum and tissue biochemistry, gene expression of some immune-related genes in the liver, and pathological lesions in some organs of Nile tilapia. Moreover, the ameliorative effects of Moringa oleifera against fish aflatoxicosis were evaluated. Fish were allocated into four groups with the M. oleifera inclusion and/or aflatoxin contamination. The groups are the first group (negative control group) fed on a basal diet without any additives, the second group (positive control group) fed on a diet containing 1 mg aflatoxin B1/kg diet. The third and fourth groups fed the contaminated diets supplemented with 0.5% and 1% M. oleifera seeds extract, respectively. Results showed the adverse effects of aflatoxin on the growth performance, serum biochemistry, liver tissue biochemistry, and gene expression and pathology of different organs of Nile tilapia. These adverse effects were ameliorated by the M. oleifera supplementation at 0.5%. In conclusion, the improvement of the biochemical and innate immune response parameters of tilapia fed diets contaminated with aflatoxin revealed the immunomodulatory role of the M. oleifera in the tilapia diet.

Inhibition of Staphylococcus aureus LC554891 by Moringa oleifera Seed Extract either Singly or in Combination with Antibiotics.

Bacterial outbreaks caused by Staphylococcus aureus (S. aureus) are interesting due to the existence of multidrug resistant (MDR) isolates. Therefore, there is a need to develop novel ways to control such MDR S. aureus. In this study, some natural agents such as honey bee (HB), extracts of either Moringa oleifera seeds (MSE), or leaves (MLE) and essential oils of garlic, clove, and moringa were studied for their inhibitory activity against this S. aureus pathogen. About 100 food samples including beef luncheon (n = 25), potato chips (n = 50), and corn flakes (n = 25) were investigated for possible pollution with the S. aureus bacteria. The isolated bacteria suspected to belong S. aureus that grew well onto Baird–Parker agar (Oxoid) and shiny halo zones and positive coagulase reaction were selected and identified by API-Kits; all of them that were approved belong to S. aureus (18 strains). The sensitivity of the obtained 18 S. aureus bacterial strains to 12 antibiotics were evaluated; all of them were resistant to ofloxacin; however, other antibiotics tested showed variable results. Interestingly, the S. aureus No. B3 isolated from beef luncheon was resistant to 10 antibiotics out of 12 ones tested. Multiple antibiotic resistance index (MAR) of this S. aureus strain was about 83.3%. Therefore, its identification was confirmed by sequencing of a 16S rRNA gene which approved a successful biochemical identification carried out by API Kits and such strain was designated S. aureus LC 554891. The genome of such strain appeared to contain mecA gene encoding methicillin resistance; it was found to contain hla, hlb, tsst-1, and finbA that encode α-blood hemolysis, β-blood hemolysis, toxic shock syndrome gene, and fibrinogen-binding protein gene, respectively. In addition, the virulence factors viz. sea; seb; sec encoding enterotoxins were detected in the DNA extracted from S. aureus B3 strain. Aqueous extract of Moringa oleifera seeds (MSE) showed inhibitory activity against S. aureus LC 554891 better than that obtained by tetracycline, essential oils or HB. Minimum inhibitory concentration (MIC) of MSE was 20µg/mL. Instrumental analysis of MSE showed 14 bioactive chemical compounds. Combinations of both MSE and tetracycline showed distinctive inhibitory activity against S. aureus LC 554891 than that obtained by either tetracycline or MSE singly.

Screening the Antibacterial Activity of Moringa oleifera Leaves and Seeds Extract against Selected Members of Bacteria

Screening the Antibacterial Activity of Moringa oleifera Leaves and Seeds Extract against Selected Members of Bacteria

Bactericidal and In Vitro Cytotoxicity of Moringa oleifera Seed Extract and Its Elemental Analysis Using Laser-Induced Breakdown Spectroscopy

In the current study, we present the correlation between the capability of laser-induced breakdown spectroscopy (LIBS) to monitor the elemental compositions of plants and their biological effects. The selected plant, Moringa oleifera, is known to harbor various minerals and vitamins useful for human health and is a potential source for pharmaceutical interventions. From this standpoint, we assessed the antibacterial and in vitro cytotoxicity of the bioactive components present in Moringa oleifera seed (MOS) extract. Detailed elemental analyses of pellets of MOSs were performed via LIBS. Furthermore, the LIBS outcome was validated using gas chromatography–mass spectrometry (GC-MS). The LIBS signal was recorded, and the presence of the essential elements (Na, Ca, Se, K, Mg, Zn, P, S, Fe and Mn) in the MOSs were examined. The bactericidal efficacy of the alcoholic MOS extract was examined against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) by agar well diffusion (AWD) assays and scanning electron microscopy (SEM), which depicted greater inhibition against Gram-positive bacteria. The validity and DNA nuclear morphology of human colorectal carcinoma cells (HCT-116) cells were evaluated via an MTT assay and DAPI staining. The MTT assay results manifested a profoundly inhibitory action of MOS extract on HCT116 cell growth. Additionally, MOS extracts produced inhibitory action in colon cancer cells (HCT-116), whereas no inhibitory action was seen using the same concentrations of MOS extract on HEK-293 cells (non-cancerous cells), suggesting that MOS extracts could be non-cytotoxic to normal cells. The antibacterial and anticancer potency of these MOS extracts could be due to the presence of various bioactive chemical complexes, such as ethyl ester and D-allose and hexadecenoic, oleic and palmitic acids, making them an ideal candidate for pharmaceutical research and applications.

Physicochemical, Microbiological and Sensory Characteristics of White Cheese Made by Adding Moringa Oleifera Seeds Extract

The aim of this study is to investigate physicochemical and sensory properties of cheese made by adding Moringa seeds extract (MSE) as coagulant. Cheese samples were processed by adding 2, 3 and 4% MSE. The results of proximate analysis indicated that moisture content was 40.8, 45.8 and 42.4%, while protein content 16.50, 16, 84 and 16.97 in cheese produced by addition of 2, 3 and 4% MSE, respectively. Statistically, significant differences (p<0.005) were found in both chemical components. The fat content (25.53, 25.72 and 25.92%) in concentrations 2, 3 and 4%, respectively. On the other hand, the highest fat content was found in cheese sample made by adding 4% MSE. The ash content increased by an increase of the concentration of MSE, and the highest value was found in cheese made by addition of 4% MSE (0.82%). Titratable acidity %, pH and The total solids (T.S%) of cheese increased significantly (p<0.005) by an increase of MSE. The mineral content of cheese was statistically affected (p<0.005) by addition of MSE. The microbiological analysis revealed that Colifrom and Salmonella were not detected in processed cheese. The sensory evaluation indicated that all cheese samples were accepted by the panelists with preference to the cheese prepared by 4% MSE. The study recommends using other parts of Moringa Oliefera in cheese coagulation and the effect of storage conditions on the quality of the cheese must be further investigated.

Application of Moringa Oleifera Seed Extract (Mose) in the Removal of Heavy Metals from Tannery Wastewater

The main focus of this research work is to investigate the possibility of applying Moringa oleifera seed extract for the removal of heavy metals from tannery wastewater. Samples were collected from effluent discharge points of the GB tannery, Muhaza tannery and Z-tannery which are located in Challawa Industrial Area, Kano, Nigeria. The samples analyzed for physico-chemical parameters are pH, temperature, hardness, conductivity, turbidity, alkalinity, total suspended solid, total dissolved solid, calcium, magnesium, chloride, nitrates, sulphates and sulphide and heavy metals such as cadmium, chromium, copper, cobbalt, iron, lead, manganese and zinc. The results show that the final levels of heavy metals after the application of Moringa Oliefera seed extracts were within NESREA acceptable limits. The heavy metal removal efficiencies were as follows; 99.29% for cadmium, chromium and iron while 96.03%, 95.56%, 96.84%, 87.41%, 91.35% for copper, cobalt, lead, manganese and zinc, respectively. The cheap, easy and environmentally friendly material called Moringa oleifera could be recommended to tannery companies for effective removal of heavy metals so as to have a safe, non-polluting environment and also prevent endangering the lives of aquatic organisms andHUMANS
 Keywords: Moringa Oleifera, tannery, wastewater, heavy metal, wastewater

CHARACTERIZATION AND EFFECTIVENESS OF MORINGA OLEIFERA SEEDS EXTRACT AS A PHOSPHATE COAGULANT

In this research, Moringa oleifera seeds extract has been shown the ability as a coagulation agent. The molecules that act as the coagulation agents were the proteins which were obtained by the extraction process of the Moringa seeds using NaCl solution. The aim of this research was to characterize and test the effectiveness of the Moringa seeds extract as a phosphate coagulant. The determination of the coagulation process was carried out using a Jar Test method of phosphate solution samples, and then effectiveness the samples were analyzed using a UV-Vis spectroscopy after-treatment of the coagulant dose variations, depositional time, and the pH variations of the samples. The characterization of the extract functional groups was determined using an FTIR spectroscopy. The results of the coagulation phosphate sample examinations showed that the optimum dose of the coagulant was 80 mL/L, with a decrease in the initial phosphate concentration of 17 ppm to 13.58 ppm. The reduction effect of precipitation time and pH also was obtained in this research. The optimum precipitation time and pH were in 30 minutes and pH 8, which reduced the phosphate concentration to 11.697 and 9.6 ppm, respectively. The FTIR spectra of Moringa oleifera seeds extracts before and after interacting with phosphate showed their differences. The interactions between the extract and phosphate were demonstrated by the appearance of buckling vibrations at wavenumbers 1064.2 cm-1, 799.8 cm-1, and 668.8 cm-1. The results of this study indicated that the Moringa seeds extraction with NaCl has potential as a phosphate coagulation agent.

Antibacterial Activity of Moringa oleifera Seeds Extract on Pathogenic Bacteria Isolated from Wastewater of Oued Bechar, Bechar Province, Southwest Algeria

The increase of drug-resistant pathogens occurring due to indiscriminate use of antibiotics and their rejection in nature are becoming a serious problem. This situation has motivated researchers across the globe to search for suita-ble alternatives in natural compounds. Moringa oleifera has been the object of several researches due to its multiple uses, as its seeds are known to possess antibacterial and antifungal properties. In the present study, pathogenic bacteria were isolated from wastewaters of Oued Bechar; the in vitro anti-bacterial activity of aqueous seeds extract against 30 strains, and the phyto-chemical screening of this plant were investigated. The results of isolated strains revealed the existence of various pathogenic bacteria dominated by: Escherichia coli, Salmonella spp, Klebsiella spp, Enterobacter spp; Streptococ-cus D, Pseudomonas spp , Vibrio spp. the inhibition diameters of aqueous seeds extract with and without oil removal ranged from 7mm to 21mm, and 8mm to 27mm respectively, with a significant antibacterial effect was ob-served against 3 of 4 Streptococci D around 1g /ml disc. Additionally, the phyto-chemical screening results showed that seeds of M. oleifera are rich in phyto-molecules such as terpenoids and tannins which are characterized by antifungal and antibacterial activity. The results of this study suggest that M. oleifera seeds from South-Western Algeria can be a source of natural anti-bacterial agent.

Stormwater Runoff Treatment Using Moringa Oleifera Seed Extract as a Natural Coagulant

Uncontrolled stormwater runoff poses a serious threat to aquatic ecosystems due the presence of harmful pollutants. Effective treatment is important prior to discharge. This study investigated the performance of Moringa oleifera seed extract as a coagulant for the reduction of turbidity, chemical oxygen demand (COD) and total suspended solids (TSS) in stormwater runoff. Stormwater samples were treated with Moringa oleifera seed extract solution at varied coagulant doses (0.5-10%), pH (3-10) and settling durations (20-60 minutes). The samples were analyzed before and after treatment to determine the coagulation efficiencies. There were improvements in stormwater quality, with up to 88%, 70% and 89% reduction in turbidity, COD and TSS levels achieved. Removal efficiencies increased with increase in coagulant dose and settling time. The results of the study indicate that Moringa oleifera can be used in the treatment of stormwater runoff for safe discharge into the aquatic environment.

Harvesting Scenedesmus obliquus via Flocculation of Moringa oleifera Seed Extract from Urban Wastewater: Proposal for the Integrated Use of Oil and Flocculant

The objectives this study were to examine the integrated use of oil–coagulant for the direct extraction of coagulant from Moringa oleifera (MO) with 5% and 10% (NH4)2SO4 extractor solution to harvest Scenedesmus obliquus cultivated in urban wastewater and to analyze the oil extracted from MO and S. obliquus. An average content of 0.47 g of coagulant and 0.5 g of oil per gram of MO was obtained. Highly efficient algal harvest, 80.33% and 72.13%, was achieved at a dose of 0.38 g L−1 and pH 8–9 for 5% and 10% extractor solutions, respectively. For values above pH 9, the harvest efficiency decreases, producing a whitish water with 10% (NH4)2SO4 solution. The oil profile (MO and S. obliquus) showed contents of SFA of 36.24–36.54%, monounsaturated fatty acids of 32.78–36.13%, and polyunsaturated fatty acids of 27.63–30.67%. The biodiesel obtained by S. obliquus and MO has poor cold flow properties, indicating possible applications limited to warm climates. For both biodiesels, good fuel ignition was observed according to the high cetane number and positive correlation with SFA and negative correlation with the degree of saturation. This supports the use of MO as a potentially harmless bioflocculant for microalgal harvest in wastewater, contributing to its treatment, and a possible source of low-cost biodiesel.

A comparative study on the efficiency of Moringa oleifera seed extract and aluminum sulfate applications as coagulants in different water treatment plants in Menoufia Governorate, Egypt

The availability of safe drinking water is very vital issue. In this study four different water plants (surface and ground) were chemically, physically and microbially tested before and after treatment. Surface water was better than ground water regard to their physical and chemical parameters. But the quality of river water is not stable due to suspended particles load caused by land development and high storm runoff during the rainy seasons. Two different coagulants were used for water coagulation and purification: Aluminum sulfate (chemical coagulant) and Moringa oleifera seeds extraction (natural coagulant), and the coagulation process was performed with and without chlorination. M. oleifera was more effective on bacteria of raw water without chlorination than aluminum sulfate. The heterotrophic plate count (HPC) was decreased by 83.6% using Moringa oleifera while with Aluminum sulfate, it decreased by 44.3 %. The total coliform bacteria using Moringa oleifera were decreased by 94.2 % while using aluminum sulfate it decreased by 34.6 %. The fecal coliform bacteria using Moringa oleifera were decreased by 98 % while with aluminum sulfate it decreased by 47.8%. The fecal streptococcus bacteria with using M. oleifera were decreased by 98.7% while using aluminum sulfate it was decreased by 60.6 %. Turbidity of raw water was 13.8 NTU and decreased to 2.2 NTU and 2.1 NTU with Moringa oleifera and aluminum sulfate, respectively. Moringa oleifera seeds extraction is non-toxic and has no side effects like aluminum sulfate and can substitute aluminum sulfate in water treatment.

Enhanced water treatment by Moringa oleifera seeds extract as the bio-coagulant: role of the extraction method

Enhanced water treatment by Moringa oleifera seeds extract as the bio-coagulant: role of the extraction method

Moringa oleifera Seed Extract Alleviates Scopolamine-Induced Learning and Memory Impairment in Mice.

The extract of Moringa oleifera seeds has been shown to possess various pharmacological properties. In the present study, we assessed the neuropharmacological effects of 70% ethanolic M. oleifera seed extract (MSE) on cognitive impairment caused by scopolamine injection in mice using the passive avoidance and Morris water maze (MWM) tests. MSE (250 or 500 mg/kg) was administered to mice by oral gavage for 7 or 14 days, and cognitive impairment was induced by intraperitoneal injection of scopolamine (4 mg/kg) for 1 or 6 days. Mice that received scopolamine alone showed impaired learning and memory retention and considerably decreased cholinergic system reactivity and neurogenesis in the hippocampus. MSE pretreatment significantly ameliorated scopolamine-induced cognitive impairment and enhanced cholinergic system reactivity and neurogenesis in the hippocampus. Additionally, the protein expressions of phosphorylated Akt, ERK1/2, and CREB in the hippocampus were significantly decreased by scopolamine, but these decreases were reversed by MSE treatment. These results suggest that MSE-induced ameliorative cognitive effects are mediated by enhancement of the cholinergic neurotransmission system and neurogenesis via activation of the Akt, ERK1/2, and CREB signaling pathways. These findings suggest that MSE could be a potent neuropharmacological drug against amnesia, and its mechanism might be modulation of cholinergic activity via the Akt, ERK1/2, and CREB signaling pathways.

Green Synthesis and Characterization of Multifunctional Zinc Oxide Nanomaterials using Extract of Moringa Oleifera Seed

Zinc oxide is highly attractive multifunctional material with its unique properties such as antifungal, antibacterial, semiconducting, UV filtering and piezo electric properties. Being nontoxic, bio safe, biocompatible material, it is used in extensive applications in biomedical, high performance nanosensors, solar cells, cosmetics as UV absorber, light-emitting diodes and piezoelectric nanogenerators.Current study focuses on the biosynthesis of zinc oxide nanoparticles by a simple, fast, cost effective and eco-friendly method using Moringa oleifera seeds extract. The synthesized zinc oxide nanoparticles were characterized using UV–visible absorption spectroscopy, Scanning Electron Microscopy, X-ray diffraction and Fourier transform infrared spectroscopy. A SEM result shows the nanoparticles synthesized are spherical shape and are considerable number of agglomerates.XRD analysis confirms the wurtzite hexagonal structure of zinc oxide nanomaterials. UV–Vis absorption spectra exhibit the sharp characteristic absorption peak of zinc oxide nanomaterials.FT-IR confirmed the presence of multifunctional zinc oxide nanomaterials and phyto-chemical constituents of Moringa oleifera seed extract.

Magnetic coagulant based on Moringa oleifera seeds extract and super paramagnetic nanoparticles: optimization of operational conditions and reuse evaluation

Magnetic coagulant based on Moringa oleifera seeds extract and super paramagnetic nanoparticles: optimization of operational conditions and reuse evaluation

Biological Control of Water Pollution at Challawa Industrial Area Kumbotso Local Government, Kano using Moringa oleifera Seed Extract

Biological Control of Water Pollution at Challawa Industrial Area Kumbotso Local Government, Kano using Moringa oleifera Seed Extract

Biological Control of Water Pollution at Challawa Industrial Area Kumbotso Local Government, Kano using Moringa oleifera Seed Extract

Biological Control of Water Pollution at Challawa Industrial Area Kumbotso Local Government, Kano using Moringa oleifera Seed Extract

Uji Aktivitas Antibakteri Campuran Ekstrak Biji Kelor (Moringa oleifera) dan Daun Kersen (Muntingia calabura) terhadap Pseudomonas aeruginosa dan Bacillus subtilis

Moringa oleifera and Muntingia calabura leaves have been reported to have an antibacterial activity that could inhibit the growth of gram positive and negative bacteria. However, the antibacterial activity of mixed extracts of M. oleifera seeds and M. calabura leaves has not been widely reported. The purpose of this study was to test antibacterial activity of the mixed extract of M. oleifera seeds and M. calabura leaves on the growth of Pseudomonas aeruginosa and Bacillus subtilis. The experiment was conducted by agar disc diffusion method using three groups of extract treatments i.e. M.oleifera seeds extract, M.calabura leaves extract, and mixed extracts of M. oleifera seeds and M. calabura leaves with a ratio of 1: 1 (v / v). The extraction of M. oleifera seeds and M. calabura leaves was conducted by soxhlation method and using ethanol as solvent. The three groups of extract treatments with a concentration of 400 ppm, 800 ppm, 1200 ppm, and 1600 ppm were tested on P. aeruginosa. The antibacterial activity test of M. oleifera seed extract against B. subtilis carried out at the level of concentrations i.e. 150 ppm, 300 ppm, 450 ppm, 600 ppm, and 750 ppm, meanwhile, M. calabura leaves extract was done at concentration 1500 ppm, 3000 ppm, 4500 ppm, 6000 ppm, and 7500 ppm. The result showed that the three groups of extract treatments possess antibacterial activity against P. aeruginosa. The mixed extracts of M. oleifera seeds and M. calabura leaves with a ratio of 1: 1 (v / v) at level concentration of 400 ppm and 800 ppm were tested against P. aeruginosa significantly increased, and at concentrations of 1200 ppm and 1600 ppm significantly decreased the inhibition diameter of bacterial growth than the other extracts treatments. The antibacterial test results of M.oleifera seeds extract and M.calabura leaves extract against B. subtilis shows that increased concentrations of the extract significantly increase the inhibition diameter of bacterial growth especially at high concentrations ( 600 ppm and 750 ppm) on M. oleifera seeds extract, as well as 6000 ppm and 7500 ppm in M. calabura leaves extract.

Application of Moringa Oleifera seed extract to treat coffee fermentation wastewater

Wastewater generated from wet processing of coffee cherries degrades stream water quality downstream of processing mills and impacts human health. The widespread popularity of coffee as an export makes this a global problem, although the immediate impact is local. Approximately 40% of all coffee around the world is wet processed, producing wastewater rich in organic nutrients that can be hazardous to aquatic systems. Moringa Oleifera Seed Extract (MOSE) offers promise as a local and affordable “appropriate” coagulation technology for aiding in the treatment of coffee wastewater. Field research was conducted at the Kauai Coffee Company to investigate the application of MOSE to treat coffee fermentation wastewater (CFW). Coagulation tests were conducted at five pH CFW levels (3–7) and MOSE doses (0–4g/L). After settling, TSS, COD, nitrate, nitrite, total nitrogen, and pH of supernatant from each test were measured. MOSE reduced TSS, COD, nitrate, and nitrite in CFW to varying degrees dependent on pH and dose applied. TSS removal ranged from 8% to 54%. Insoluble COD removal ranged from 26% to 100% and total COD removal ranged from 1% to 25%. Nitrate and nitrite reduction ranged from 20% to 100%.

English

In order to evaluate the efficiency of sludge dewatering, specific parameters such as settling velocity (Vs) and sludge volume index (SVI) have to be measured. However, problems arise when these parameters are used for the evaluation of efficiency using M. oleifera as natural phytocoagulant or sludge conditioner. Using statistical optimization, it was found that despite good results of Vs and SVI, the concentration of residual suspended solids in supernatant liquid or turbidity was very high. Thus, turbidity of supernatant liquid was selected as a criteria to evaluate the efficiency of dewatering process. In this research, two optimization steps were run under two factors for each optimization; i.e., mixing time and concentration of M. oleifera seeds extract with NaCl (1 M). The range of these factors was (100 – 1000 mg/L) for M. oleifera seeds extract concentration, and (5 – 30 min) for mixing time. In the first optimization, Vs and turbidity were used as responses. While in the second optimization SVI and turbidity were used as response parameters. Both optimization steps were run under (pH = 7), mixing speed (120 rpm) for the first minute, and (40 rpm) during the rest period of experiment. By using Design-Expert software v9, for the first optimization of settling velocity, the optimum Vs was found to be (1 cm/min) and the turbidity of the supernatant was (350.7 NTU). Whereas in the optimization of the sludge volume index (SVI), the optimum value was (24.7 mL/g), corresponding turbidity value of suspended solids in supernatant liquid was (341.5 NTU). Since the turbidity was very high, second optimization by redesigning the factors was conducted resulting in optimum values of dosage of (462.8 mg/L), mixing time of (13.4 min), turbidity of (67.2 NTU) (further (80.2 %) reduction compared to the (350.7 NTU) of the first optimization) and settling velocity of (0.93 cm/min) were obtained.For the optimization of SVI, dosage of (447.5 mg/L) and mixing time of (8.3 min) gave (33.5 mL/g) of SVI with (67.2 NTU). These optimized dewatering parameters can be used to improve the efficiency of sludge dewatering.