Oleifera Leaf Extract Research Articles

Dietary inclusion of Moringa oleifera leaf extracts as alternatives to antibiotic growth promoter on live performance, carcase traits, physical meat quality, and health status of broiler chickens

Dietary inclusion of Moringa oleifera leaf extracts as alternatives to antibiotic growth promoter on live performance, carcase traits, physical meat quality, and health status of broiler chickens

Formulation and Quality Analysis of Yoghurts Fortified with Mentha spicata, Moringa oleifera, and Murraya koenigii Leaf Extracts

The aim of this study was to investigate the impact of incorporating Mentha spicata (mint), Moringa oleifera (moringa), and Murraya koenigii (curry) leaf extracts into yoghurt to enhance its nutritional and therapeutic benefits. The study assessed the sensory, physicochemical, microbiological, and antibacterial properties of the fortified yoghurts along with control. Among the four yoghurt samples, curry leaf extract-fortified yoghurt has the fastest setting time (4.5 h), highest protein content (3.42%) and lowest syneresis (8%) and was the most preferred in sensory analysis, excelling in color, texture, and overall acceptability (p=0.05). It also showed the strongest antibacterial activity against Salmonella and Staphylococcus aureus. All yoghurt samples were found to be safe for consumption. The incorporation of leaf extracts significantly improved the growth of lactic acid bacteria, sensory qualities and functional benefits of yoghurt, making them promising ingredients for developing health-promoting beverages. However, there is a need for optimization when using these extracts to reduce its adverse taste effects, particularly at higher concentrations.

Antibacterial efficacy of Moringa oleifera leaf extract against methicillin-Resistant Staphylococcus aureus isolated from a patient with wound abscesses in Shaafi Hospital, Mogadishu, Somalia

Antibacterial efficacy of Moringa oleifera leaf extract against methicillin-Resistant Staphylococcus aureus isolated from a patient with wound abscesses in Shaafi Hospital, Mogadishu, Somalia

Moringa oleifera, Cyclea barbata, Centella asiatica Leaves Extract Intervention Ability to Improve Fetus Development and Blood Protein Levels in Malnutrition Pregnant Model Mice

Background: Pregnant women experiencing chronic energy deficiency are at risk of giving birth to low birth weight and premature (stunted) babies. Objectives: In an effort to prevent stunting, the study investigates the effects of a 1:1:1 ratio of Moringa oleifera, Cyclea barbata, and Centella asiatica leaf extract on fetal growth and blood protein levels. Methods: This research used a laboratory experimental approach with 25 adult female Mus musculus Balb/C mice as a model of malnourished pregnancy, was divided into 5 groups: pre-test, positive control, negative control, placebo and test group. Moringa oleifera, Cyclea barbata, and Centella asiatica leaf extracts are administered to the test group and positive control in 1:1:1 ratio. Results: The study results indicated that in pregnant mice without malnutrition receiving a mixture of Moringa oleifera, Cyclea barbata, and Centella asiatica leaf extracts, the average fetal body length was 9.7% higher and their weight was 7.4% heavier than pregnant mice with experiencing chronic energy deficiency. Conclusions: The administration of Moringa oleifera, Cyclea barbata, and Centella asiatica leaf extract can contribute to improving fetal development and blood protein levels. However, its effectiveness is more pronounced in normal pregnancies than in pregnancies with chronic energy deficiency as it can prevent stunting.

Effect of ethanol leaf extract of Moringa oleifera on D-galactose induced changes in lipid profile of aged Wistar rats

Uncontrolled hyperlipidaemia is linked to some age-related diseases like obesity, diabetes mellitus, and cardiovascular diseases. To examine the blood lipid profile in wistar rats, this study employed D-galactose-induced aging as a model. The lipid profiles were analysed using standard methods. Thirty (30) male albino rats were randomly assigned into five (5) groups of six (6) rats each and fed with basal diets. Group 1 and 5 which served as the young and adult controls respectively received basal diet only while the others were administered D-galactose (150mg/kg) and Moringa oleifera extract (MOLE-200mg/kg and 400mg/kg) respectively for eight (8) weeks. At the end of the study rats were sacrificed and blood samples were collected. At week 0, the results of the total cholesterol concentrations (98.81 ± 6.43), TAG (69.15 ± 1.34), LDL-cholesterol (31.80 ± 4.10), VLDL-cholesterol (13.83 ± 0..27) and HDL-cholesterol (35.64 ± 2.81) in rats administered M. oleifera leaf extract, D-galactose and distilled water respectively showed no significant (P > 0.05) difference across all the groups but at week 8 treatment with high dose of MOLE resulted in a significant (P ≤ 0.05) decrease in total serum cholesterol (112.45 ± 3.05), TAG (114.05 ± 5.47), LDL-cholesterol (19.66 ± 4.06) concentrations relative to the D-galactose only treated group. Conversely, HDL-cholesterol concentration was significantly increased (P ≤ 0.05) following treatment with both low (69.53 ± 1.94) and high dose (69.99 ± 2.64) of MOLE relative to the D-galactose (63.65 ± 2.54) treated group. These results suggest that Moringa oleifera leaf extract exerts an anti-hyperlipidaemic effect against D-galactose-induced toxicity and thus could confer protection against aging.

Moringa oleifera leaf extract ameliorates collagen degradation via the inhibition of MMP‐3 expression in UVB‐induced rats

Prolonged exposure to high‐intensity UVB induces the formation of reactive oxygen species (ROS) in skin tissue, triggering an increase in matrix metalloproteinase‐3 (MMP‐3) enzyme production and leading to collagen degradation. Moringa oleifera (MO) contains bioactive compounds known for ROS‐scavenging and anti‐inflammatory properties. However, the precise molecular mechanism of action remains unclear, requiring the inhibition of MMP‐3 activation and regulation of collagen deposition. This study aims to elucidate the potential effect of MO leaf extract‐based gel in restoring collagen deposition by reducing MMP‐3 activation in UVB irradiate‐induced collagen loss in rats. This study employed a completely randomized design, comprising four groups: a healthy group without UVB radiation, a negative control group subjected to UVB radiation and receiving a placebo, and two treatment groups exposed to UVB radiation with 5% or 10% moringa leaf extract‐based gel (MO‐5% or MO‐10%), respectively. Results showed that MO‐5% and MO‐10% significantly reduced MMP‐3 gene expression and increased collagen density compared to the negative control group (p < 0.05). Moringa oleifera leaf extract ameliorates collagen degradation by inhibiting MMP‐3 expression in UVB‐induced rats, suggesting its potential as a pharmacological and cosmetic agent for UVB‐induced skin damage.

Enhanced supercapacitive performance in Cu doped Sn3O4 synthesized by a simple extract route

The synthesis of nanomaterials using plant extracts is of great importance for a sustainable, chemical-free future. In this study, multivalent Sn3O4 doped with Ni and Cu were synthesized by a simple, low-cost method using Moringa oleifera leaf extract at low temperature and investigated for supercapacitor applications. Various characterizations indicate that the synthesized samples crystallize in a triclinic space group and have a larger surface area of 109.2 m2/g, which is advantageous for electron and ion transport. Electrochemical studies were carried out by cyclic voltammetry, galvanostatic charge/discharge, impedance spectroscopy and cycle life measurements in 6 M KOH electrolyte. Cu-doped Sn3O4 showed the highest capacitance of 608 C/g at a current density of 1 A/g. An asymmetric device built with it delivered an energy density of 31.5 Wh/Kg at a power density of 1400 W/Kg and retained 85 % capacity even after 6000 continuous cycles. The results show that extract-based synthesis of multivalent oxides is beneficial not only for a sustainable environment but also for achieving superior capacity.

UV-Protection Effect of Nanoemulgel Formulation from Moringa oleifera Lam. Leaf Extracts in Rattus novergicus

Exposure to sunlight (UV radiation) can harm the skin, leading to cancer and tissue damage. The body’s natural defense involves activating hormones that stimulate melanin synthesis to reduce cellular damage. Moringa oleifera Lam. leaves, rich in flavonoids and phenolic compounds, are promising UV-protection agents. This study assessed the efficacy of nanoemulgel formulations containing M. oleifera leaf extract through in vitro and in vivo assays. Twenty-five rats were divided into five groups, including formulations with 2%, 3%, and 4% M. oleifera extract, a negative control, and a positive control with Azarine Sunscreen Gel SPF 45. The nanoemulsions had appropriate size and quality, with the highest SPF observed at 4% concentration (7.95±0.06). According to FDA classification, these formulations fall within the 'extra' SPF category (6<SPF<8). However, melanin scores in the nanoemulgel groups were comparable to the untreated control, and the 4% concentration (44.4±16.3) did not match the protection provided by commercial sunscreen (10.0±1.58). These findings suggest M. oleifera has potential for photoprotection, but further optimization is needed.

Hermetic effect of Moringa oleifera leaf extract mitigates salinity stress in maize by modulating photosynthetic efficiency, and antioxidant activities

Salinity poses a significant constraint to cereal productivity particularly in arid and semiarid regions. The application of allelochemical has shown promising results in mitigating the intensity of abiotic stresses. A pot experiment was conducted to assess the efficacy of different concentrations of aqueous allelopathic extract derived from moringa leaves in mitigating the adverse impacts of salinity on the germination and growth of maize cultivars via seed priming. The study involved three variables: two cultivars of maize, ‘Pioneer 30Y87’ (salt tolerant) and ‘Pioneer 30T60’ (salt sensitive) e seed priming with moringa leaf extract (MLE) at varying concentrations of 0.5%, 1.0%, 1.5%, 2.0%, 2.5%, 3.0%, and hydro-priming as control; and different salinity levels of 0, 6, and 12 dS m-1. Salinity had a negative impact on the germination process, leading to delayed and suboptimal growth of seedlings. Additionally, salinity reduced the synthesis of photosynthetic pigments (20-50%), photosynthesis, transpiration, internal carbon, and stomatal conductance. Further, MLE also improved the antioxidant activities (catalase: CAT and peroxidase: POD) by 22-56% which reduced the hydrogen peroxide production. Moreover, ‘P-30Y87’ exhibited favorable performance in terms of better germination, growth, photosynthesis and antioxidant activities. The application of moringa leaf extract (3%) resulted in a more notable hermetic effect in elevating salinity stress thereby enhancing germination, growth, photosynthesis and antioxidant activities. In the conclusion, application of MLE (3%) is a promising approach to mitigate the adverse impacts of salinity by improving germination, growth, photosynthesis and antioxidant activities.

Antifungal activity of moringa (Moringa oleifera) leaf extracts against major plant pathogens

Antifungal activity of moringa (Moringa oleifera) leaf extracts against major plant pathogens

The Effects of Moringa Leaf Extract on the Growth and Microbial Load of Boiler

The adverse effect of microbes in poultry production, especially broiler chicken remains a major concern for poultry breeders. This study determined the effects of Moringa leaf (Moringa Oleifera) extract on the performance and microbial loads of broiler birds. A total of 25 broiler chicks were used and separated into 5 groups in an iron pen with 5 chicks in each group. Group 1 (control, with no treatment), group 2 (received a treatment of 62.5mg/L), group 3 (received a treatment of 125mg/L), group 4 (received a treatment of 250mg/L), group 5 (received a treatment of 500mg/L). After 4 weeks of treatment, the faeces from each group, the control group inclusive were collected for microbial analysis. The result showed that the Moringa Oleifera leaf extract at all doses recorded non-significant difference in the parameters analysed among the groups. It was also noticed that the disparities of the results were great. Some as much as 11.5 and 168. 37 standard error of mean. Moreover, Group 3, receiving 125mg/L, exhibited a substantial reduction in E. coli, salmonella, total bacteria count, and pseudomonas activities compared to other groups. Group 3 had the highest water consumption. Amongst the treatment group, group 3 had the highest increase in weight. In conclusion, Moringa leaf extract effectively lowered the microbial load in broiler chicks at the treatment of 125mg/L without any adverse effects. Keywords: Moringa Oleifera, Moringa leaf, microbial load, broiler birds.

EFFECT OF MORINGA (Moringa oleifera) LEAF EXTRACT ON IN VITRO INHIBITION OF Candida albicans BIOFILM

Background: Oral candidiasis is an opportunistic infection in the oral cavity. This infection is caused by the fungus Candida albicans. The most important virulent attribute of this fungus is its ability to form biofilms, which can adhere to mucosa, epithelial lining, organs, prostheses or dentures. The formed biofilm is resistant to antifungal drugs. Objectives: The purpose of this study was to determine the effect of Moringa (Moringa oleifera) leaf extract on the inhibition of Candida albicans biofilm formation in vitro. Methods: This type of research is experimental study using post tests with control group design. Fungus used in this study was Candida albicans ATCC 10231. Determination of minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were conducted by microdilution method. Inhibition test of C. albicans biofilm formation was carried out using the polystyrene microplate assay method. Concentration of extracts used in this study were 12.5%, 6.25%, 3.13% and 1.57%. Inhibitory activity of biofilm was measured using a crystal violet (CV) assay. Results: The results showed that MIC of Moringa leaf extract against the fungus C. albicans was at a concentration of 6.25%. Moringa leaf extract starting at a concentration of 3.13% already has had ability to inhibit formation of C. albicans biofilm. Conclusion: It can be concluded that Moringa leaf extract can inhibit formation of Candida albicans biofilm, so it can be developed as an alternative herbal ingredient to prevent oral candidiasis.EFFECT OF MORINGA (Moringa oleifera) LEAF EXTRACT ON IN VITRO INHIBITION OF Candida albicans BIOFILM

Antibacterial, antioxidant and anticoagulant activities of different aged Moringa oleifera leaf extracts and application in natural serum product

Antibacterial, antioxidant and anticoagulant activities of different aged Moringa oleifera leaf extracts and application in natural serum product

Assessing the usefulness of Moringa oleifera leaf extract and zeatin as a biostimulant in enhancing growth, phytohormones, antioxidant enzymes and osmoprotectants of wheat plant under salinity stress

Assessing the usefulness of Moringa oleifera leaf extract and zeatin as a biostimulant in enhancing growth, phytohormones, antioxidant enzymes and osmoprotectants of wheat plant under salinity stress

Phytocure drug alternatives to manage antimicrobial resistance in poultry transmitted human pathogens

ABSTRACT This research aimed to explore natural, cost-effective alternatives to conventional antibiotics, focusing on Citrus sinensis peel and Moringa oleifera leaf extracts. The objectives were to extract, characterize, and evaluate the antimicrobial potential of these extracts against common poultry pathogens. The proximate analysis of M. oleifera leaf powder revealed 22% protein, 10% fat, 10% ash, and 10% fiber. Phytochemical screening of both aqueous and ethanol extracts of M. oleifera and orange peel indicated the presence of alkaloids, flavonoids, saponins, tannins, and total phenolics. These extracts exhibited significant antimicrobial activity against bacterial strains like E. coli, S. gallinarum, P. aeruginosa, B. cereus, Campylobacter, S. aureus and M. gallisepticum. In a study involving 30 broilers, those treated with M. oleifera extract showed superior antibacterial effects compared to other treatment groups. These findings suggest that M. oleifera leaf extract could serve as an effective, affordable alternative to antibiotics in broiler diets, potentially improving disease management in the poultry industry.

Facile green synthesis of plant-mediated selenium nanoparticles (SeNPs) using Moringa oleifera leaf and bark extract for targeting α-amylase and α-glucosidase enzymes in diabetes management

In recent years, nanoparticle synthesis innovations have focused on developing simple, cost-effective, non-toxic, and eco-friendly methods. This study investigates the green synthesis of selenium nanoparticles (SeNPs) using aqueous extracts of Moringa oleifera leaves (ML) and bark (MB) via the precipitation method. The synthesized SeNPs were characterized using Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). TEM analysis revealed the formation of approximately spherical particles with average sizes ranging from 10.47 nm to 28.5 nm for SeNPs synthesized with ML (SeNPsML) and 15.47 nm–32.83 nm for those with MB (SeNPsMB). XRD analysis indicated that SeNPsMB were predominantly crystalline, while SeNPsML were mainly amorphous with a few crystalline peaks. Morphological studies showed spherically shaped particles with a high degree of agglomeration, evenly distributed throughout the matrix. SeNPsMB exhibited higher dose-dependent inhibition of α-amylase and α-glucosidase compared to SeNPsML, likely due to differences in nanoparticle size and form. These findings suggest that SeNPsMB may possess superior antidiabetic activity.

Effects of Moringa oleifera leaf extract on growth performance, blood hematology, immunity, carcass, and meat quality of Mae Hong Son chicken

Moringa oleifera is a nutrient-rich plant with a wide range of beneficial effects on chicken and chicken products. Mae Hong Son chickens, a variety of Thai native poultry, are renowned for their exceptional meat quality, distinct flavor, and remarkable tenderness. Therefore, this present study aimed to investigate the effects of Moringa oleifera leaf extract (MOLE) on growth performance, blood hematology and immunity, carcass, and meat quality of Mae Hong Son chickens, respectively. Briefly, 200 birds of Mae Hong Son chickens were subsequently divided into 4 groups, each containing 50 birds. They were fed for 16 weeks with different supplementary as follows: 1. Control group 2. Placebo (maltodextrin + microcrystalline cellulose PH101) 3. 2% of MOLE extract supplementation and 4. 4% of MOLE extract supplementation. The results showed that T4 achieved the best outcomes in terms of body weight and average daily gain, while also having the lowest total white blood cell (WBC) count and the highest ND HI titer at 12 weeks old (p < 0.05). On the other hand, T1 had the highest fat percentage and thiobarbituric acid reactive substances (TBARS) values in both the thigh and breast (p < 0.05). Additionally, the shear force of T1 was the lowest, both in the thigh and breast (p < 0.05). In summary, the potential of MOLE could improve growth performance, hematological parameters, immune response, and meat quality in Thai native chicken production.

Green synthesis of Fe[sbnd]Ni[sbnd]Sn trimetallic oxides from Moringa oleifera for photocatalytic degradation of Reactive Red 6B dye

The nanocomposite FeNiSn trimetallic oxides were synthesized using an aqueous Moringa Oleifera leaf extract. The prepared nanocomposite was analyzed using SEM, FTIR, XRD, N2 adsorption-desorption, Zeta potential, and UV–vis analysis. A common Azo dye named Reactive Red 6B (RR-6B), widely applied in the dyeing industry and causing environmental pollution, was selected to examine the photodegradation of FeNiSn trimetallic oxides nanocomposite under sunlight irradiation, and it was shown that this dye could be successfully degraded by prepared nanocomposite. Effects of parameters such as inorganic salt concentration, H2O2 concentration, contact time, pH, and impact of different concentrations of catalyst and dye were examined to find out degradation conditions. This prepared nanocomposite was applied for the photodegradation of RR-6B dye, and 95 % degradation efficiency was obtained at pH 4, 10 mg of catalyst concentration, 10 ppm of dye concentration, and contact time of 2 h. Degradation kinetics were carried out using Langmuir-Hinshelwood and first-order reaction models. These results showed a facile technique for forming nanocomposite to efficiently degrade organic contaminants from wastewater, which directly impacts industrial wastewater treatment. The antibacterial activity was performed using the Time assay kill method for Gram-positive S. aureus and Gram-negative E. coli bacteria. The results of antibacterial activity showed an inhibition effect on bacterial growth by trimetallic oxides NPs, suggesting their potential use in antimicrobial coatings and materials. These findings underscore the practical applications of our research, demonstrating its relevance and potential impact in real-world scenarios.

Novel green synthesis approach of eco-friendly magnetic/semiconductor nanocomposites as magnetically separable and reusable photocatalyst for organic dye degradation

Eco-friendly and effective separation of photocatalytic nanoparticles from the reaction mixture is crucial to facilitate their repeated use in photocatalysis. The development of magnetic/semiconductor nanocomposites with high catalytic performance and easy separation is an efficient strategy for wastewater treatments. In this study, we explore the potential of combination of magnetic (CoFe2O4) and semiconductor (ZnS) synthesized via a green approach using Moringa oleifera leaf extract as magnetically separable photocatalysts for dye degradation. X-ray diffraction analysis confirmed the presence of the cubic spinel ferrite phase of CoFe2O4 and the cubic zinc blend phase of ZnS within the nanocomposites. The micrographs illustrated nearly spherical nanoparticles with average particle sizes of 12 nm for CoFe2O4 and 26 nm for CoFe2O4/ZnS, respectively. The presence of SO suggested the incorporation of ZnS on the surface of CoFe2O4/ZnS nanocomposites. The UV–visible spectra revealed an increase in the band gap energy from 3.7 to 4.5 eV with increasing ZnS concentrations. Magnetic properties analysis signified that the CoFe2O4/ZnS nanocomposites exhibited ferromagnetic characteristics. The optimal degradation efficiency of photodegradation methylene blue was observed for CoFe2O4/ZnS 50 %, achieving 78.3 % degradation within 80 min with intervals 20 min. The magnetically separable capability allows for the recycling of nanocomposites after three consecutive cycles. Therefore, CoFe2O4/ZnS nanocomposites emerge as promising candidates as separable photocatalysts for the removal of organic pollutants in environments.

Antiviral Properties of Moringa oleifera Leaf Extracts against Respiratory Viruses.

Moringa oleifera (M. oleifera) is a plant widely used for its beneficial properties both in medical and non-medical fields. Because they produce bioactive metabolites, plants are a major resource for drug discovery. In this study, two different cultivars of leaves of M. oleifera (Salento and Barletta) were obtained by maceration or microwave-assisted extraction (MAE). We demonstrated that extracts obtained by MAE exhibited a lower cytotoxic profile compared to those obtained by maceration at concentrations ranged from 25 to 400 µg/mL, on both Vero CCL-81 and Vero/SLAM cells. We examined their antiviral properties against two viruses, i.e., the human coronavirus 229E (HCoV-229E) and measles virus (MeV), which are both responsible for respiratory infections. The extracts were able to inhibit the infection of both viruses and strongly prevented their attack and entry into the cells in a range of concentrations from 50 to 12 µg/mL. Particularly active was the variety of Salento that registered a 50% inhibitory concentration (IC50) at 21 µg/mL for HCoV-229E and at 6 µg/mL for MeV. We identified the presence of several compounds through high performance liquid chromatography (HPLC); in particular, chlorogenic and neochlorogenic acids, quercetin 3-O-β-d-glucopyranoside (QGP), and glucomoringin (GM) were mainly observed. In the end, M. oleifera can be considered a promising candidate for combating viral infections with a very strong action in the early stages of viral life cycle, probably by destructuring the viral particles blocking the virus-cell fusion.