Olea Ferruginea Research Articles

PHYTOCHEMICAL ANALYSIS AND FT-IR OF LEAF AND STEM EXTRACTS OF OLEA FERRUGINEA ROYLE


 
 
 The latest study was performed to investigate the ethno pharmacological potential of ethnobotanical significant plant, Olea ferruginea Royle that is a member of the Oleaceae family. The stem of this plant and leaf powders were macerated in different (polar and non-polar) solvents. The phytochemical study showed the occurrence of saponins, alkaloids, anthraquinonines, flavonoids, minimizing sugars, cardiac glycosides, tannins and terpenoids in reasonable quantities in Olea ferruginea, as verified by Fourier Transform Infrared (FT-IR) analysis. Based on the findings of the current research, the traditional use of this targeted plant of the Oleaceae family as food, fodder, feed, and medicinal seems acceptable and therefore justifiable.
 
 

Medicinal plants used as therapeutic medicine in the Himalayan region of Azad Jammu and Kashmir

• Medicinal plants have been playing an important role in traditional healing systems. • A total of 125 plant species used in herbal medicine were identified. • The herbaceous used life form is dominantly in herbal preparation. • The maximum ICF index value is calculated for liver and digestive problems. • Berberis lyceum and Thymus sarphylum (100% FL) are used for treating jaundice and joint pain respectively. The current research is the first quantitative ethno-botanical assessment of district Haveli in the Himalayan region, a remote and undiscovered territory of Azad Jammu & Kashmir (AJK). The people of the Himalayan region have a wealth of traditional knowledge that can be used to cure a variety of diseases. However, ethno-medicinal data must be recorded as soon as possible in order to preserve them and prevent extinction due to oral transmission, as well as to determine which remedies are still in use. Data was gathered from 70 informants (25 female and 45 male) through semi-structured interviews and group discussions. Informants were chosen at random or, in certain cases, using the snow-ball approach. To evaluate the gathered information, different ethnobotanical indices including UV, RFC, FL%, and ICF were used. Moreover, the information gathered in the current research work was contrasted with 22 different studies of aligned areas, including Pakistan. A total of 125 plant species used in herbal medicine were identified, which were distributed among 56 families and employed in 16 illness categories. Asteraceae (16 spp.) was the most used family in the area. The herbaceous life form was dominantly (66 spp., 53.65%) used in herbal preparation. The most commonly used part in herbal medicine was leaves (29.55%), and the most generally used method for preparing herbal recipes was extract (26.53%). The maximum ICF index value (0.92) was calculated for liver and digestive problems. The highest UV (0.92) was calculated for Berginia virgata. Olea ferruginea had the highest relative importance (100). Two plant species, Berberis lycium and Thymus surphylum, had 100% fidelity value. A high similarity was found with the study conducted on Devi Gali and Neelum valley with JI of 16.75 and 14.58. The plants with the highest levels of UV, RFC, RI, and FL would be examined for future pharmacological study in order to validate the findings and produce novel medications. Local residents have remained closer to nature due to their lack of communication with modern civilization. Despite the fact that they continued to employ medicinal plants in different herbal recipes, this tradition is on the verge of extinction since it is mostly retained by hakims, midwives, or the elderly people. The new generation is much less interested in traditional health practices due to advanced healthcare facilities and modern lifestyles.

Survival and Growth of Olive Tree (Olea africana) Seedlings Under Open Field Conditions is Enhanced in Hydrogel Amended Soils Media, Teso Subcounty, Kenya

The rapid decline of timber from natural forests against increasing domestic demand for wood products has caused a steady increase in timber prices in Kenya. Recently, research and development institutions have recognized the ecological and economic value of indigenous tree species and therefore have emphasized the importance of their future production on private smallholder farms rather than in large plantations. This situation has been worsened by climate changes such as the frequent high intensity of prolonged droughts and the nature of soils with poor water retention capacity. As a result, unplanted forest regeneration sites have increased hence a need for re-afforestation of these sites to meet the target for the plantation industry. Therefore, there is a need for mass production of Olea africana seedlings for reforestation programs. One such approach is the application of hydrogels or synthetic polymer organic combinations capable of improving the water and nutrient retention of the soil that can support seedling growth. This study sought to investigate the effects of hydrogel application on selected growth characteristics of O. africana. Seeds were germinated and transferred into two sets of tubes of the same size arranged in a complete randomized block design (CRBD) experiment. The sets were subjected to different levels of hydrogel concentration. Under (0gm as control) and 7gm, 14gm, and 21gm) concentration levels of hydrogel mixed with soil in open field conditions. The height and shoot collar diameter of germinated seedlings were measured every two weeks for two months. The survival of O. africana seedlings decreased with time for all treatments irrespective of the addition of hydrogels with the highest (85%) survival of seedlings on soils mixed with 21gm of hydrogel compared to 0gm hydrogel that recorded (15% survival) eight weeks after transplanting. Application of different levels of hydrogel on soils had a significant influence on the survival of transplanted O. africana seedlings (p˂0.05). Generally, the height of O. africana seedlings increased with time for all types of treatments (0, 7, 14 and 21gm of hydrogel); however, the rate of growth of seedlings was highest (0.667cm /week) on soils treated with 21gm of hydrogel and least (0.33cm/week) on 0gm hydrogel. The shoot collar diameter of O. africana seedlings increased with time for all types of treatments (0, 7, 14 and 21gm of hydrogel); however, the average shoot collar diameter of O. africana seedlings was highest (0.088mm/week) on soils inoculated with 21gm hydrogel and least (0.066mm/week) on soils not inoculated with hydrogel. These results imply that a higher dose of hydrogel significantly enhances the survival and growth characteristics (height and shoot collar diameter) of transplanted seedlings by improving the water-holding capacity of soil and making it available for plant uptake during dry conditions. Hydrogels can therefore be used to amend soils for a sustainable mass regeneration of tropical timber species for reforestation programs, increased forest cover and restoration of biodiversity in Kenya

Impact of alternate bearing on proximate composition of Olea ferruginea Royle fruits collected from Zhob, Balochistan, Pakistan

Impact of alternate bearing on proximate composition of Olea ferruginea Royle fruits collected from Zhob, Balochistan, Pakistan

Ecosystem Services and Linkages of Naturally Managed Monotheca buxifolia (Falc.) A. DC. Forests with Local Communities across Contiguous Mountainous Ranges in Pakistan.

Simple SummaryMonotheca buxifolia is a wild fruit yielding tree species of the Sapotaceae family, frequently reported in northern and western parts of Pakistan. This species is highly involved in providing a number of services, including provisioning, regulation, maintenance and cultural indicating the close relations between society and the protection of mountain areas. Due to the poor socio-economic situation and natural hazards in this region, Monotheca phytocoenoses have been heavily exploited during the last few decades. Apart from the dominant species, we also report some other important tree species (Juglans regia, Pinus roxburghii, Ficus palmata, Punica granatum, Olea ferruginea, and Acacia modesta, etc.) that play a key role in improving the economic situation of the mountain inhabitants. These phytocoenoses should be properly managed on a priority basis to support long-term consumption.The local community of the Suleiman and Hindukush mountain systems in Pakistan has largely depended on the natural resources of the environment since ancient times. The ecosystem of these regions is under huge pressure due to a lack of awareness and the uncontrolled interference of communal, commercial, security, political, and ecological conditions. The present study was designed to illuminate the link between mountain society and the consumption of the benefits from Monotheca phytocoenoses using the ecosystem services concept from the sphere of the socio-ecological system to cultural relations. The use of this approach is very important due to the visible role and dominant status of Monotheca vegetation within the ecological system of the region. M. buxifolia is strongly connected with both local and cultural traditions and is counted as a key species, particularly for high-mountain inhabitants. We report that Monotheca phytocoenoses provide several services including shelter, food, fodder, medicines, and wood, etc., to the indigenous community and is highly valued in the local culture because of the poor economic condition of the society. The concept of this cultural keystone species is crucial for understanding ecosystem services and must be considered for the protection and conservation of these habitats. The results of field and social studies have shown that the stable maintenance of Monotheca phytocoenosis forests ensures the existence of key species as the most important providers of ecosystem services, e.g., provisioning, regulation, maintenance and cultural services, indicating the close relations between society and the protection of mountain areas. According to the results obtained, the mountains community of the studied area believes that tree species like M. buxifolia, F. palmata, O. ferruginea, P. granatum, A. modesta, J. regia, etc., are the key components contributing to the function of both the mountain ecosystem and communities’ well-being. This approach will be extremely useful for ensuring an inclusive management of the socio-ecological system of the Hindukush and Suleiman Mountain ranges of Pakistan.

Antidiabetic Potential and Antioxidant Activity of Olea europaea subsp. Cuspidata (Indian Olive) Seed Extracts.

The aim of the present study was to evaluate the antioxidant and antidiabetic potential of Indian olive seed extracts. Plant seeds were sequentially extracted with n-hexane, chloroform, methanol, and water. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) scavenging and alpha-amylase inhibitory activities of extracts were carried out. Olea europaea methanolic extract (MEOE) and aqueous extract (AEOE) were orally administered to normoglycemic and alloxan-treated diabetic rats so as to determine their hypoglycemic effect. High-performance liquid chromatography (HPLC) analysis showed gallic acid, ferulic acid, quercetin, and vanillic acid in MEOE. It was found that the methanolic and aqueous extracts exhibited the maximum DPPH and alpha-amylase inhibition activities, respectively. MEOE and AEOE exerted a significant decline in the fasting blood sugar in diabetic animals (p < 0.05); however, they did not cause hypoglycemia in nondiabetic animals. Treatment with MEOE and AEOE reduced the aggravated liver and kidney function biomarkers. Aggravated levels of oxidative stress biomarkers including superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH), and malondialdehyde (MDA) were restored by treatment with MEOE. Moreover, MEOE improved the count of islets of Langerhans in the pancreas, fatty changes, and enlarged sinusoidal spaces in the liver and necrosis of the glomerulus and tubular cells of the kidney in diabetic rats. This study showed that the African olive seed extract effectively managed experimental diabetes and restored the normal functions and histology of the liver and kidney in diabetic rats through the reduction of oxidative stress.

Ethnobotanical study of medicinal plants of Pashat Valley, Bajaur, along Pakistan–Afghanistan border: a mountainous region of the Hindu Kush Range

Medicinal plants are the primary and effective source to cure various human ailments throughout the globe. The remote area of the Pashat valley at the Pakistan–Afghanistan border is still unexplored to document ethnomedicinal knowledge and practices. In the current study, the contemporary investigation recorded the native acquaintance of remedial plants. A total of 73 plant species belonging to 68 genera and 46 families were used by traditional communities for the treatment of 40 different kinds of human diseases including digestive, respiratory, urinary, skeletal, muscular systems, etc. The family Lamiaceae was found dominant with 9 species followed by Leguminosae and Solanaceae with 4 species each, Euphorbiaceae, Moraceae, Rhamnaceae and Rosaceae with three species each. The most frequently used parts of plants were leaves (35.7%) followed by fruits (22.4%), seeds (12.2%), roots (7.14%) and plants as a whole (6.12%). Whereas the remedy preparation approaches were in powder form (28.46%), decoction (26.15%), extract (20%) and a paste (11.54%). The quantitative ethnobotanical indices i.e. used value (UV), relative frequency citation (RFC), family importance value (FIV) and Jaccard index (JI) were calculated for all plant species. The used value (UV = 0.04) was found highest for Berberis lyceum, Myrtus communis, Punica granatum and Dodonea viscosa. Similarly, the maximum RFC value of 0.25 was investigated for Foeniculum vulgare and Olea ferruginea each. Besides, the highest FIV of 96.08% and 58.17% values were calculated for the family Lamiaceae and Solanaceae. This study shows that the corresponding area is rich in medicinal plants and could contribute to ethnomedicinal knowledge. The collected data will establish a baseline for the comprehensive investigation of active molecular constituents of the medicinal flora of the area. Soon, the exploration of these medicinal plants could discover new herbal drugs in the field of pharmacology.

Preliminary Phytochemical Composition and In Vitro Anthelmintic Activity of Aqueous and Ethanol Extracts of Olea africana against Mixed Gastrointestinal Worms in Dogs.

Olea africana is used by some indigenous communities in Kenya to control gastrointestinal worms in animals. Plant-based anthelmintics are gaining popularity globally in the control of gastrointestinal worms in animals. The egg hatch inhibition assay was used to assess the in vitro anthelmintic efficacy of aqueous and ethanol leaf extracts of O. africana against the eggs of mixed gastrointestinal helminths in dogs. Probit regression was used to calculate the concentration of extracts that inhibited egg hatching by 50% (IC50). Albendazole was used as a control. Standard techniques were used to quantify the phytochemicals in the extracts. The aqueous extract had an IC50 of 1.85 mg/mL (1.64–2.10), and the ethanol extract had an IC50 of 0.25 mg/mL (0.23–0.26). Quantitative phytochemical analysis revealed that aqueous and ethanol extracts of O. africana contained alkaloids (19.40 and 61.60%), saponins (24.00 and 6.00%), phenols (0.95 and 1.28 mg/g gallic acid equivalents (GAE)), flavonoids (8.71 and 12.26 mg/g catechin equivalents (CE)), and tannins (67.30 and 76.30 mg/g of tannic acid equivalent (TAE)), respectively. O. africana has dose-dependent anthelmintic effects against mixed gastrointestinal worms in dogs. These findings support the traditional use of Olea africana as a treatment option for gastrointestinal worms in dogs.

Comparative Study of Antimicrobial and Antioxidant Potential of Olea ferruginea Fruit Extract and Its Mediated Selenium Nanoparticles.

Nanotechnology, the science of the recent era, has diverse applications in agriculture. Selenium (Se) is a non-metal and an essential micronutrient for animals and humans. In this study, selenium nanoparticles (SeNPs) were biosynthesized by using Olea ferruginea fruit extracts. The size, shape, chemical nature, and identification of functional groups involved in the synthesis of SeNPs were studied by UV-visible spectroscopy, Scanning Electron Microscope (SEM), and Fourier Transform Infra-Red (FTIR) spectrometry. SeNP synthesis was confirmed by an absorption peak at 258 nm by UV-visible spectroscopy. SEM showed that SeNPs were spherical, smooth, and between 60 and 80 nm in size. FTIR spectrometry confirmed the presence of terpenes, alcohols, ketones, aldehydes, and esters as well as phyto-constituents, such as alkaloids and flavonoids, that possibly act as reducing or capping agents of SeNPs in an aqueous solution of Olea ferruginea. Antimicrobial activity was examined against bacterial pathogens, such as Klebsiella pneumonia, Escherichia coli, Staphylococcus aureus, and Staphylococcus epidermitis, as well as fungal pathogens, such as Aspergillus niger and Fusarium oxysporum, by using the well-diffusion method. Antioxidant activity was observed using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, ABTs assay, and reducing power assay. At a higher concentration of 400 ppm, biosynthesized SeNPs showed an inhibition zone of 20.5 mm, 20 mm, 21 mm, and 18.5 mm against Klebsiella pneumonia, Escherichia coli, Staphylococcus aureus, and Staphylococcus epidermitis, respectively. Similarly, SeNPs also demonstrated a zone of inhibition against Aspergillus niger and Fusarium oxysporum of 17.5 and 21 mm, respectively. In contrast to Olea ferruginea fruit extracts, Olea ferruginea-mediated SeNPs demonstrated strong antimicrobial activity. By performing the DPPH, ABTs, and reducing power assay, SeNPs showed 85.2 ± 0.009, 81.12 ± 0.007, and 80.37 ± 0.0035% radical scavenging potential, respectively. The present study could contribute to the drug development and nutraceutical industries.

Ambophily in Olea ferruginea: a transitional state in the pollination syndrome

A number of plants species believed to be wind pollinated (anemophilous) are actually pollinated both by wind as well as insects (ambophily) because all the traits of the two pollination modes have not evolved simultaneously. Comparatively few angiosperms exhibit ambophily and the significance of such a mechanism is not fully understood. During the present investigation, ambophily was studied in Olea ferruginea, over a period of three years. It is an andromonoecious and out-crossing tree species of the olive complex. The species is predominately pollinated by wind because it shows fruit set even when the insect pollinators were excluded. Moreover, a substantial amount of pollen was observed on the body parts of insect visitors and, comparatively, fruit set was enhanced in open-pollinated flowers receiving insect visitors. Floral structure of O. ferruginea displays floral traits favoring both wind and insect pollination. Furthermore, the flowers of this species were found to be visited by 20 insect species classified under three orders with Diptera and Hymenoptera being the leading pollen foragers. The results of linear regression of the hanging slide experiment showed that the dispersal of pollen grains decreased significantly with increasing distance from the canopy and the pollen density increased significantly with an increase in insect activity. The present work provides an insight to understand the ecological and evolutionary adaptations and consequences of spatio-temporal variation in plant – pollinator interactions. It is, therefore, useful for addressing both basic and applied questions in community structure and function including the evolution of floral traits, reproductive assurance, and the development of optimal conservation strategies.

Phenolic profiling and antioxidant evaluation of extracts from Southern African indigenous fruits byproducts

The call to build climate-resilient food systems in Africa has revived interest in indigenous fruits, which, however, remain under-researched. In this study, the phenolic content and antioxidant profiles of seed and pulp of ethanolic extracts from eight Southern African indigenous fruits were evaluated using UPLC-ESI-Q-TOF/MS and four antioxidant assays. Total phenols and hydroxycinnamic acids were highest in Dovyalis caffra (Kei apple) seed (5084.5 and 3403.83 mg/kg). Flavonoids were most abundant in Colpoon compressum (Colpoon) seed (1127.23 mg/kg), while hydrolysable tannins were highest in Syzygium guineense (Water pear) seed (666.13 mg/kg). Proanthocyanidins were abundant in Harpephyllum caffrum (Wild plum) pulp while anthocyanins were highest in Olea africana (Wild olive) pulp. Hierarchical clustering heatmap analysis showed similar concentration and diversity in the composition of reported compounds. Syzygium guineense seed had the lowest DPPH values. ORAC values were highest in O. africana pulp while H. caffrum pulp had the highest FRAP values and lipoxygenase inhibition capacity. In conclusion, the study revealed a diverse profile of phenolics in indigenous fruits extracts, to which their bioactivity is attributed. Specifically, H. caffrum pulp and S. guineense seed have potential as natural sources of phenolic antioxidants for food application.

Antibacterial, Hemagglutination, and Insecticidal Activity Studies on the Solvent Extracts of the Roots of Olea ferruginea

Olea ferruginea has been used to treat skin ailments, as well as kidney and ocular problems for a long time. The current study was designed with the aim of investigating and scientifically validating its widespread use. Chloroform, n-hexane, and ethyl acetate were used to assess the antibacterial, hemagglutination, and insecticidal properties of O. ferruginea roots. Escherichia coli, Micrococcus luteus, Klebsiella pneumoniae, and Pseudomonas aeruginosa were among the bacterial strains selected for assessing antibacterial activity. The results showed that ethyl-acetate (EtOAc) extract (56%) and chloroform (CHCl3) extract (56%) showed the widest zone of inhibition against K. pneumonia, while n-hexane extract (13.4%) had the lowest zone of inhibition against P. aeruginosa. All three extracts remained inactive against M. luteus. During hemagglutination activity, the CHCl3 and EtOAc extracts, when used at different concentrations, only agglutinated the AB−ve and O+ve blood groups, respectively, while the n-hexane extract strongly agglutinated the A−ve and B−ve blood groups at different concentrations. The plant extracts were also checked for insecticidal activity against Rhizopertha dominica. The results also revealed the high mortality rate of CHCl3 extract (70%) against R. dominica as compared to other extracts. The aforesaid activities suggest that the roots of O. ferruginea have excellent medicinal viability and contain a wide variety of agglutinins and lectins, as shown in this study.

A Systematic Review of Medicinal Plants of Kenya used in the Management of Bacterial Infections.

Kenya's vision 2030 partly aims at ensuring adequate health care for all, and the integration of traditional healthcare practices into the national healthcare system would present a more rapid alternative towards the realization of universal health coverage in Kenya. Currently, research on Kenyan medicinal plants with potential antibacterial activity remains vastly fragmented across numerous literature studies and databases; thus, it is imperative to collate and appraise these data for the ease of future research and possible clinical application. Objective. This review aims at exploring and compiling research evidence on medicinal plants used in the management of bacterial infections in Kenya, with a focus on their efficacy and safety. Methodology. A comprehensive web-based systematic review using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines was executed to highlight the Kenyan medicinal plants used for the management of bacterial infections in Kenya. This review includes studies published until January 2021 from the PubMed, Science Direct, AJOL, and Google Scholar databases. Results. A total of 105 Kenyan medicinal plants belonging to 43 families have their in vitro activity against various human pathogenic bacteria evaluated. Plants from the Lamiaceae, Rutaceae, and Fabaceae families were the most commonly studied. Aloe secundiflora, Toddalia asiatica, Senna didymobotrya, Warbugia ugandensis, Tithonia diversifolia, Fuerstia africana, Olea africana, and Harrisonia abyssinica were the plants frequently evaluated within Kenya. The plants with the strongest antimicrobial activities were Toddalia asiatica, Hagenia abyssinica, Ocimum gratissimum, Harrisonia abyssinica, Senna didymobotrya, Olea Africana, Camellia sinensis, and Tarmarindus indica. Conclusion. Based on a published work, it is evident that traditional medicine is seemingly an acceptable and efficient system among Kenyan communities in the management of bacterial infections. Kenya's rich biodiversity with diverse secondary metabolites presents a promising source of new therapeutic alternatives with possibly different mechanisms of action against bacteria.

COMPARATIVE STUDY OF FOREST COVER AND CARBON STOCK CONCERNING CLIMATE CHANGE; A CASE STUDY OF MARGALLA HILLS NATIONAL PARK, ISLAMABAD, PAKISTAN

In the global carbon reserves, the forest is one of the central reserves. Humans face critical challenges with the intensification of ozone gases in the atmosphere. The study conducted in Margalla Hills National Park (MHNP), Islamabad capital territory, has analyzed environmental changes and carbon stock estimation. This area has multiple species types: Acacia Modesta, Quercus incana, Pinus roxburghii, Dodonaea viscosa, Olea ferruginea, Ziziphus mauritiana, etc. Remote sensing (RS) and Geographic Information System (GIS) technology were used to illustrate Land use land cover (LULC), Contour, Slope, Aspect, and total Rainfall calculation.&#x0D; LULC data of different years from 1997 to 2019 for every five years were analyzed. The analysis results showed that in Margalla Hills National Park, during the year 1997, total settlements were on about 145 hectares. Dense forest had an area of about 7262 ha, rocks were on about 5920 ha, barren land was about 2680 ha, and water bodies on about 38 ha By 2005, the Settlement area increased by about 877 ha in 8 years. While the increase noted in the dense forest was about 1000 ha. Findings reveal that water bodies in MHNP decreased by about 37 hectares. Furthermore, by 2010, the Settlement area was increased, and the areas of barren land and water bodies decreased. It is evaluated that during the year 1997 northeastern part of the study area had a higher amount of vegetation than the NDVI results. Whereas, within eight years, the same portion contains less vegetation. Rainfall results were analyzed for 2019, which shows that rainfall in MHNP increased. Sixty-seven species are enlisted in MHNP, and a detailed inventory was prepared for the first time. The study results can be helpful in the analysis of future impacts of these developments on the land cover and C stocks in the forest ecosystem.

Species Diversity, Growing Stock Variables and Carbon Mitigation Potential in the Phytocoenosis of Monotheca buxifolia Forests along Altitudinal Gradient across Pakistan

The sub-tropical broadleaved forests in Pakistan are the main constituents of the ecosystem services playing a vital role in the global carbon cycle. Monotheca buxifolia (Falc.) A. DC. is an important constituent of these forests, encompassing a variety of ecological and commercial uses. To our best knowledge, no quantitative studies have been conducted in these forests across the landscape to establish a baseline for future monitoring. We investigated the forest structural attributes, growing stock characteristics and total biomass carbon stock and established relationships among them in the phytocoenosis of Monotheca forests along an altitudinal gradient in Pakistan to expand an eco-systemic model for assessment of the originally-implemented conservation strategies. A floristic survey recorded 4986 individuals of 27 species in overstory and 59 species in the understory stratum. Species richness (ANOVA; F = 3.239; p = 0.045) and Simpson’s diversity (ANOVA; F = 2.802; p = 0.043) differed significantly in three altitudinal zones, with a maximum value for lower elevations, followed by middle and higher elevations. Based on the importance values, Acacia modesta and Olea ferruginea are strong companions of M. buxifolia at lower and higher altitudes, whereas forests at mid elevation represent pure crop of M. buxifolia (IVI = ≥85.85%). A similar pattern in stem density, volume and Basal area were also recorded. The carbon stock in trees stratum (51.81 T ha−1) and understory vegetation (0.148 T ha−1) contributes high values in the lower elevation forests. In contrast, soil carbon had maximum values at higher elevation (36.21 T ha−1) and minimum at lower elevation (16.69 T ha−1) zones. Aboveground biomass carbon stock (AGB BMC) of woody trees, understory vegetation and soil organic carbon (SOC) were estimated higher (77.72 T ha−1) at higher and lower (68.65 T ha−1) elevations. Likewise, the AGB BMC exhibited a significant (p &lt; 0.05) negative correlation with elevation and positive correlation with soil carbon. We concluded that lower elevation forests are more diverse and floristically rich in comparison to higher altitudinal forests. Similarly, the biomass carbon of Monotheca forests were recorded maximum at low altitudes followed by high and middle ranges, respectively.

Antioxidant and Cytotoxic Activity of a New Ferruginan A from Olea ferruginea: In Vitro and In Silico Studies.

Studies of the ethyl acetate extract bark extract of Olea ferruginea led to the isolation of one new compound Ferruginan A (1) in addition to two known compounds, Ferruginan (2) and cycloolivil (3). Structures of the isolated compounds were confirmed by mass spectrometry (MS) and NMR spectral data. The ethyl acetate fraction and compounds (1–3) were evaluated against breast cancer cell line (MCF-7) and as antioxidants using the free radical scavenging assay. Results revealed that compound 2 exhibits significant antioxidant activity with an IC50 value of 21.74 μg/mL. In addition, the ethyl acetate fraction showed good cytotoxic activity (79.31% inhibition at 250 μg/mL), whereas compounds 1–3 exerted mild cytotoxic activity (IC50 = 8.03–12.01 μg/mL) as compared to the standard (IC50 = 4.41 μg/mL) against MCF-7. Docking studies suggested that antioxidant activity is due to the chelation of compounds with copper present in the active site of tyrosinase. These results suggest that the extract exhibits considerable antioxidant activity, and the isolated compounds exert moderate anticancer activity.

Adaptation Trial and Growth Performance of Multipurpose Trees/Shrubs Species at Meti Site of Kellem Wollega Zone, Oromia, Ethiopia

This study was conducted at Meti Site of Haro Sabu Agricultural research center, Oromia. The objective of this study was to evaluate the adaptability and growth performance of different multipurpose trees/shrubs species and select or screen those best performed and adapted to the area. For this seedling of: Acacia senegal, Pinus patula, Callistemon citrinus, Olea africana and Spathodea nilotica were out planted on a plot of 14m*14m. The space between block of each species was 3m and Spacing between rows and within row was based on the recommendations of each species. In order to fit the given objectives growth parameter data of trees/shrubs (height, root collar diameter (only for trees&lt; 1.3m in height), diameter at breast height (for trees ≥1.3m height) and survival rate) were collected by an interval of 6 months and Analyses of variance and LSD tests were employed to assess the results at p=0.05 using Minitab 17. As a result, the higher mean height growth was observed on Callistemon citrinus ( 2.59 m) , Spathoda nilotica ( 2.48 m) and Pinus patula ( 2.34 m). Again Gains in root collar diameter (RCD) was significantly highest on Pinus patula (37.75cm, 2.81cm) and Spathodea nilotica (32.08cm, 3.52cm).The Mean survivals of all trees grown at site were good ranging from 85.36% ( Pinus patula) to 100% ( Spathodea nilotica ( 100%). Generally this finding may help forest managers (stakeholder) to allocate species into the site that grow and adapt well. To select the most adaptable ones for such areas , use them at wider scale and increase their importance in the overall agricultural productivity (soil conservation, timber production, shading purpose, etc) and in general multifunction purposes in the area, further testing of provenances of the best performing species is recommended. Keywords: Growth performance, Meti, Relative growth rate in height (RGRH), Root collar diameter (RCD), Survival percentage. DOI: 10.7176/JEES/11-11-03 Publication date: November 30 th 2021

Assessment of metals content of widely used traditional toothbrushes in Addis Ababa, Ethiopia

ABSTRACT. Traditional toothbrushes are used by the vast majority of people who cannot afford to buy the commercial toothbrush and toothpaste. The traditional toothbrushes are generally obtained from any slim woody part of a toothbrush tree. The main purpose of this study was to determine selected metals (Ca, Fe, Mg, Al, Cu, Cr, Ni, Zn, Mn, Pb, and Cd) in traditional toothbrushes obtained from three plants including Ligustrum vulgare L., Phoenix reclinata and Olea africana, which are extensively used in Addis Ababa, Ethiopia, by using microwave plasma-atomic emission spectroscopy (MP-AES) after wet digestion. Recoveries of the metals in spiked samples varied from 90.4–107%. The overall mean concentrations determined (mg/kg, dry weight) were in the ranges of Ca (4267–36514) &gt; Fe (131–318) &gt; Al (81.6 –224) &gt; Mg (45.6–122) &gt; Zn (27.2–175) &gt; Mn (20.1–29) &gt; Cu (6.6–20.3) &gt; Cr (6.7–8.9) &gt; Ni (2.6–7.9). Analysis of variance at 95% indicated significant differences in the metals’ contents of three toothbrushes. The results indicated that the selected traditional toothbrushes are good sources of essential metals and free from Pb and Cd. Therefore, the investigated Ethiopian traditional toothbrushes are found to be safe for human use.&#x0D; &#x0D; KEY WORDS: Metal contents, Traditional toothbrushes, Wild privet (Ligustrum vulgare), Wild date palm (Phoenix reclinata), African wild olive (Olea africana), Ethiopia&#x0D; &#x0D; Bull. Chem. Soc. Ethiop. 2021, 35(2), 257-272.&#x0D; DOI: https://dx.doi.org/10.4314/bcse.v35i2.4

Green synthesis of silver nanoparticles of olea ferruginea root extract: Characterizations, biological assays and their catalytic application in dye degradation

In current work, silver nanoparticles (AgNPs) were prepared by a rapid biogenic technique “Green method” utilizing root extract of Olea ferruginea. The synthesized nanoparticles were characterized for UV visible spectroscopy, Fourier Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Scanning electron microscopy (SEM), High Resolution Transmission Electron Microscopy Studies (HRTEM) and biological assays. UV visible absorption spectroscopy confirms the formation of AgNPs by giving Surface Plasmon Resonance (SPR) peak at 430 nm. FTIR study showed occurrence of various functional groups present in root extract of Olea ferruginea. X-ray diffraction analysis determined the crystalline nature of bio-fabricated silver nanoparticles. The elemental composition of green made silver nanoparticles was studied through Energy Dispersive Spectroscopy (EDS) analysis. SEM &amp; HRTEM study revealed the size, shape, surface morphology &amp; dispersion level of molecules. The biologically synthesized AgNPs showed high antimicrobial, antifungal and antioxidant activity. The AgNPs are observed to be an excellent catalyst on reduction of hazardous dyes, which is confirmed by a decrease in absorbance of maximum values.

Effect of climate warming on seedling growth and biomass accumulation of Acacia modesta and Olea ferruginea in a subtropical scrub forest of Pakistan

ABSTRACT This study investigated the short-term impacts of projected climate warming on two dominant tree species of the Hayat-ul-Mir subtropical scrub forest of Pakistan. Seedlings of Acacia modesta and Olea ferruginea were exposed to ambient (To) and warming temperature (To + 2.3°C and To + 4.5°C) with control (M15%) and elevated (M20%) soil moisture in plant growth chamber for 90 days. To + 4.5°C induced detrimental effects on both species inhibiting seed germination and reducing their growth rate, biomass accumulation and allocation, however promoted development of root nodules (12 ± 4,0 nodules/root) in A. modesta. To + 2.3°C with M20% was beneficial for O. ferruginea with significant increase in stem height (4.8 ± 1.4 cm), elongated roots (31.2 ± 3.4 cm), branch development (9 ± 1.7), biomass accumulation (8.69 ± 0.4 g) and higher foliar nutrient concentration but for A. modesta it only promoted root elongation (16.5 ± 1.3 cm) and biomass accumulation in stem (1.05 ± 0.1 g) and foliage (0.08 ± 0.001 g). A. modesta grew better under To with significantly high germination and survival rate. A future 2.3°C warming could be ecologically significant to induce shifts in the structure and function of the Hayat-ul-Mir forest.