Okra Extract Research Articles

Evaluation of Physical Attributes and Efficacy of Lip Balm Formulated with Okra Fruit Extract (Abelmoschus esculentus (L.) Moench)

The okra fruit (Abelmoschus esculentus (L.) Moench) is renowned for its significant antioxidant properties, attributed to its rich content of bioactive compounds. Despite its high antioxidant activity, which has been extensively documented, its potential as a component in cosmetic formulations particularly lip balm preparation remains underutilized. This study seeks to address this gap by incorporating okra fruit extract into lip balm formulations and evaluating the resultant physical and functional attributes. Lip balm formulations were developed with three varying concentrations of okra fruit extract: F1 (1%), F2 (3%), and F3 (5%). A series of comprehensive evaluations were conducted to assess the physical stability and performance of the lip balms, including organoleptic properties, homogeneity, pH, melting point, spreadability, and overall stability. Stability was monitored over a 28-day period at room temperature to simulate typical storage conditions. The antioxidant efficacy of the okra extract was quantified using the DPPH (1,1-diphenyl-2-picrylhydrazyl) assay, which yielded an IC50 value of 10.06 ppm, reflecting its potent free radical scavenging capacity. The lip balm formulations exhibited a distinct okra aroma, smooth texture, and uniform color variations ranging from light brown (F1), brown (F2), to dark brown (F3). pH values ranged from 5.40 to 5.81, and melting points were between 49.6°C and 52.9°C, all within the acceptable range as per SNI 16-4769-1998 standards. ANOVA statistical analysis revealed no significant changes (p > 0.05) in melting point and spreadability over time. However, significant differences (p < 0.05) were noted in pH values, with further analysis indicating that formulations F2 and F3 differed significantly from F1 (p < 0.05). These results demonstrate that okra fruit extract not only enhances the antioxidant properties of lip balm but also maintains its stability and functionality, supporting its potential as a valuable ingredient in cosmetic formulations.

Okra extract alleviates lipopolysaccharide-induced inflammation response through the regulation of bile acids, the receptor-mediated pathway, and gut microbiota.

Okra contains flavonoids and vitamin C as antioxidants and it contains polysaccharides as immunomodulators. Flavonoids regulate the inflammatory response in mice and may be related to gut microbiota. This study therefore aimed to investigate the impact of okra extract (OE) on inflammation in mice and to elucidate its underlying mechanism. Forty male Kunming (KM) mice were categorized into four groups: the control (CON) group, the lipopolysaccharide stimulation (LPS) group, the 5 mg mL-1 OE intervention (LPS + OE) group, and the 5 mg mL-1 OE supplementation plus mixed antibiotics (LPS + OE + ABX) group. The results showed that, compared with the OE group, the expression of inflammatory signaling pathway genes was upregulated and gut barrier genes were inhibited in the OE + ABX group. The Fxr receptor was activated and the abundance of Akkermansia was increased after OE supplementation, whereas the effect was reversed in the OE + ABX group. Meanwhile, Fxr was correlated positively with Akkermansia. The OE supplementation alleviated the inflammatory response in mice under LPS stimulation, accompanied by changes in gut microbiota and bile acid receptors, whereas the addition of antibiotics caused a disturbance to the gut microbiota in the OE group, thus reducing the effect of OE in alleviating the inflammatory response. © 2024 Society of Chemical Industry.

Intervention with Purple Okra Pudding and Supplement to Improve Antioxidant Status in Healthy Adults

This research aimed to analyze the potency of purple okra-based products in improving the antioxidant status of healthy adults. Thirty adults with high body fat percentages were allocated into three groups: the first group was treated with 100 g of purple okra pudding/day, the second group was provided with a purple okra extract supplement that contained 3.80 g of extract/day, and the third group was a control group. The intervention was carried out for 28 days. All subjects were exposed to nutrition education, and data on their characteristics, food intake, and physical activity level were collected. The results showed that purple okra pudding and supplement had antioxidant activity of 0.39 and 455.39 mg AEAC/g extract, IC50 of 543.79 and 71.78 ppm, and total phenol of 6.21 and 24.49 mg GAE/g extract, respectively. There were significant differences among subject groups in energy, protein, and fat intake as well as physical activity level. The group treated with purple okra pudding showed a significantly higher ΔSOD after the intervention, most probably due to the role of antioxidants contained in purple okra in upregulating antioxidant defense. In contrary, there was a declining trend of ΔSOD in the group treated with purple okra extract supplement. The different effects observed between the two groups might be due to the different phenol contents between the two intervention products. This study showed that purple okra has the potential as a functional food and health supplement in improving the antioxidant status of healthy adults with high body fat percentages as indicated by a higher change of SOD level (0.08 u/mL) in comparison to the control (-0.07 u/mL).

Effects of dried okra extract on lipid profile, renal function and some RAGE-related inflammatory genes expression in patients with diabetic nephropathy: A randomized controlled trial

BackgroundDiabetic nephropathy (DN) is a common complication of type 2 diabetes. Okra (Abelmoschus esculentus L) is reported to have anti-diabetic effects. The present study aimed to investigate the effects of dried okra extract (DOE) supplementation on lipid profile, renal function indices, and expression of inflammatory genes, as well as serum level of soluble Receptor for Advanced glycation end products (sRAGE) in patients with DN. MethodsIn this triple-blind randomized placebo-controlled clinical trial, 64 eligible patients with DN received either 125 mg of DOE or placebo daily along with DN-related nutritional recommendations for 10 weeks. Changes in kidney indices including proteinuria and estimated glomerular filtration rate (eGFR), lipid profile, serum SRAGE, as well as the expression of RAGE, ICAM-1, and IL-1 genes were measured over 10 weeks. ResultsAfter adjustment for the potential confounders, between-group analyses showed no significant differences in terms of lipid profile, kidney function indices, sRAGE, and RAGE-related inflammatory genes expression after 10 weeks. ConclusionDaily 125 mg DOE along with nutritional recommendations on top of usual care did not lead to significant changes in renal function indices, lipid profile, and inflammatory genes expression in patients with DN.

Increased expression of GLUT4, catalase and nitric oxide by a crystalised fraction from ethanolic extracts of oak leaf lettuce and okra seed in C2C12 cell line

The fractions obtained from low temperature-induced crystallisation of ethanolic extracts of green- and red-oak leaf and okra seed were accounted for total polyphenol content and the DPPH antioxidant activity, resulting in 5.99, 4.44, and 12.35 mg gallic acid equivalent per g sample, and 0.45, 0.35, and 0.99 mg Trolox equivalent per g sample, respectively. Insulin resistance was the result after incubating C2C12 skeletal muscle cells in high glucose DMEM for 20 h. Oxidative stress and inflammation were triggered by re-incubating these resistant cells with insulin. The expression of p-p38 MAPK and NF-B proteins and the NF-B p65 transcription factor activity were enhanced. Such inflammatory protein markers were reduced when the crystalised extracts replaced insulin. Increased catalase activity and NO production were also determined for the incubations using the crystalised extracts. It was suitable to include these vegetables in the daily diets of prediabetic individuals for better management of the patient’s health status by increasing insulin sensitivity and decreasing inflammation.

Influence of Okra Extract Supplementation on Some Haematological Parameters of Male Mice Exposed to Aflatoxin

This research was directed to determine the influence of an alcoholic extract of okra on the lessening of the destructive impact of the aflatoxin produced by Aspergillus fumigatus in white mice and its influence on some physiological blood parameters. Different food samples, (grains and fruits) such as (wheat, barley, corn, rice, citrus, strawberries, and apples) were selected for the isolation of a variety of fungi. The results showed that Aspergillus flavus 15(18.7%), Aspergillus niger12(15%), Penicillium spp 7(8.7%), Aspergillus terreus 7 (8.7%), Aspergillus fumigatus7(8.7%), Alternaria spp. 10 (12.5%), Aspergillus parasiticus 6 (7.5%) Fusarium 6 (7.5%), Penicillium chrysogenum5(6.3), Mucor spp.2(2.5%),and Rhizopus stoloinfier 3(5.5%).The identified fungi were tested for aflatoxin production, and the results revealed that Aspergillus fumigatus produced the most aflatoxin. Okra alcoholic extract was tested in vivo against the negative impact of aflatoxins using different concentrations. The findings revealed that alcoholic extracts showed reasonable influence on some blood parameters, and the results are promising.

Okra [Abelmoschus esculentus (L.) Moench] improved blood glucose and restored histopathological alterations in splenic tissues in a rat model with streptozotocin-induced type 1 diabetes through CD8+ T cells and NF-kβ expression.

Diabetes mellitus is a complex metabolic syndrome that involves dysfunction of spleen and other lymphoid organs. Medicinal plants, including okra (Abelmoschus esculentus (L.) Moench), were used widely for diabetes treatment. Scarce data are available about the potential anti-diabetic effects of okra, the histopathological alterations in splenic tissues and the mechanistic pathways underlying this association. The current research investigated the effects of okra pod extract on the biochemical parameters and expression of CD8+ T cells and nuclear factor kappa (NF-k) B and releasing proinflammatory cytokines in spleen in streptozotocin (STZ)-induced diabetic rat models. A total of 50 mature male Wister albino rats were divided into five isolated groups; the first served as control (untreated) animals, the second (DM group) diabetes induced by STZ (at a dose of 45 mg/kg body weight, administered intraperitoneally), the third group (DM + Insulin): diabetic rats administered insulin subcutaneously (10 units/kg bw/day) daily for 4 weeks, the fourth group was administrated 400 mg/kg okra extract daily for 4 weeks, and diabetic induced rats in the fifth group were administrated 400 mg/kg okra extract daily for 4 weeks. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity in Abelmoschus esculentus (L.) Moench was studied, and the content of phenolic compounds in okra pods was estimated using high-performance liquid chromatography. Diabetes induction led to decreased body weight, increased blood glucose levels. Capsular thickness was significantly increased, white pulp was widely dispersed, and mature lymphocytes in the periphery were also drastically decreased, with thick follicular arteries, necrosis, and depletion of lymphocytes in the germinal center. Red pulp revealed severe congestion and degenerative changes, deposition of hemosiderin granules and lymphocytic depletion. In addition, collagen fiber deposition was increased also in this group. The induction of diabetes exaggerated NF-kβ expression and mediated downregulation of the expression of CD8+ T cells in spleen tissue. Interestingly, oral administration of okra extracts post diabetes induction could mitigate and reverse such adverse effects. Altogether, our study points out the potential benefits of okra in improving blood glucose levels and restoring histopathological alterations in splenic tissues through CD8+ T cells and NF-kβ expression in a diabetic rat model.

Microencapsulation of Phenolic Compounds Extracted from Okra (Abelmoschus esculentus L.) Leaves, Fruits and Seeds

Several plants rich in phenolic compounds have many uses in the food and pharmaceutical fields. However, after extraction, these active biomolecules are susceptible to degradation. Microencapsulation is a possible solution to prevent this rapid degradation. In this study, phenolic compounds from the okra Abelmoschus esculentus L’s leaves, fruits and seeds were extracted using microwave-assisted extraction and then microencapsulated via the spray drying technique using maltodextrin combined with pectin (in a ratio of 10:1) as an encapsulation material. The total phenolic content, DPPH scavenging and antimicrobial activities of okra extracts and encapsulated samples were evaluated to verify the encapsulation efficiency. Particle size distribution determination and scanning electron microscopy of the microcapsules were also carried-out. The ethanolic leaf extract showed higher significant levels of total phenolic compounds (162.46 ± 4.48 mg GAE/g DW), and anti-oxidant (75.65%) and antibacterial activities compared to those of other aqueous and ethanolic extracts from fruits and seeds. Furthermore, the spray-dried ethanolic leaf extract had the highest total phenolic content. However, the encapsulated ethanolic fruit extract had the highest percentage of DPPH scavenging activity (30.36% ± 1.49). In addition, antibacterial activity measurements showed that the addition of ethanolic and aqueous seed microcapsules provided a significant zone of inhibition against the bacterium Brochotrix thermosphacta (38 mm and 30 mm, respectively). Okra aqueous leaf microcapsules showed the smallest Sauter mean diameter values (7.98 ± 0.12 µm). These data are applicable for expanding the use of okra leaves, fruits and seeds as food additives and/or preservatives in the food industry.

Analysis of natural okra extracts as corrosion inhibitors for mild steel in acidic medium.

Plant extracts have been shown to effectively inhibit metal corrosion. Using the Box-Behnken design, gravimetric, and electrochemical techniques, analyses were designed to investigate the anti-corrosion potential of okra in a 1M HCl medium. The inhibition performances derived from the various methods were in good agreement, demonstrating that physio-chemisorption was effective and adhered to the Langmuir isotherm model. The efficiency of okra mucilage extract was 96% at a much lower concentration compared to 91.2% and 88.4% for the unsieved extract and gelly-okra filtrate, respectively. FTIR results showed the presence of several functional groups in the okra mucilage extract that are associated with adsorption, and TGA analysis revealed that the extract has high thermal stability. FESEM analysis also supported evidence of adsorption. It was determined that corrosion inhibition by okra mucilage extract was primarily influenced by temperature, followed by extract concentration, with immersion time having the least effect. From the model optimization, it was observed that okra mucilage extract at 200 ppm, 60°C, and 24 h gave an inhibition efficiency of 89.98% and high desirability. These results demonstrate the high capacity of natural okra as an efficient biodegradable corrosion inhibitor.

Nutraceutical Potential of Encapsulated Purple Okra (Abelmoschus Esculentus L. Moench) Extract

The accumulation of free radical production impacts on the development of degenerative diseases which are the highest cause of morbidity and mortality in the world. Biofortification of purple okra in the form of encapsulated extract has the potential as a nutraceutical through the role of antioxidants. This study aimed to analyze the potential of encapsulated purple okra extract as a nutraceutical by determining physical-chemical characteristics, microbial and heavy metals contamination, antioxidant capacity, total flavonoids, and quercetin. This was a laboratory experimental study on purple okra which was extracted using the maceration method. The results of physical-chemical characteristics showed that purple okra extract has the form of dry powder, greenish-brown color, odor characteristic of okra extract (caramel-like), and sour taste with yield of 4%, pH of 4.8, undetectable solvent residue, water content of 13.5%, total ash content of 10.4%, and disintegration time of 1.25 minutes. The results of microbial contamination showed a total plate count of 3.1 102 CFU g⁻¹, yeast and mold count, E. coli, S. aureus negative CFU g⁻¹, and Salmonella spp negative CFU 10g⁻¹. The results of heavy metal contamination showed that As, Pb, Cd, and Hg were not detected every mg Kg⁻¹. The results of antioxidant capacity, total flavonoids, and suspected quercetin derivatives showed a value of 84.88%, 81.32 mg QE g⁻¹, and 4.91 mg g⁻¹. These bioactive components act as free radical scavengers in helping to prevent chain reactions. Encapsulated purple okra extract has shown its potential as a nutraceutical that helps prevent degenerative diseases.

Performance of Trichoderma harzianum and Bacillus amyloliquefaciens Bioformulation on Germination and Expression of Bioprotective Molecules against Cercosporiosis in Abelmoschus esculentus in the Field

The present study aimed at assessing the performance of the formulation combining Trichoderma harzianum and Bacillus amyloliquefaciens on germination and induction of the synthesis of bioprotective molecules against pathogens in Abelmoschus esculentus causing cercosporiosis. A significant difference (p = 0.05) was revealed between the formulation (T1) and the control (T0) in the field on the seed germination rate of okra varieties Clemson spineless (V1) (83.3 vs 94.4%; 88.8 vs 100%; 77.7 vs 88.8%) and Hire (V2) (77.7 vs 94.4%; 88.8 vs 100%; 83.3 vs 88.8%) in agroecological areas V, III and IV respectively. The same effect observed on plant health with bioformulation was significant compared with the control in terms of protection against cercosporiosis pathogens. The protection ranges according to treatment and control for the two okra varieties in agroecological areas V, III and IV were 79.24 vs. 86.48%; 100 vs. 100%; 77.76 vs. 91.82% for variety V1 and 88.88 vs. 94.44%; 94.44 vs. 100%; 83.33 vs. 94.44% for variety V2. Gas chromatography combined with mass spectrometry revealed an overproduction of bioactive molecules in the hydro-methanolic extracts of okra leaves treated with the formulation, which are responsible for its bioprotection in the three agroecological areas. In conclusion, the use of the Trichoderma harzianum and Bacillus amyloliquefaciens formulation on okra crops significantly induced germination and systemic resistance of Abelmoschus esculentus plants against cercosporiosis agents.

Verifying the Optimal Extraction Method and Content of Red Okra Extracts and Crude Pectin Using Response Surface Methodology (RSM)

Recently, many studies have reported the properties and functionality of okra pectin. However, these studies are about green okra pods, and pectin in red okra pods, stems, and roots has not been reported. Therefore, this study aimed to optimize the red okra extraction method using response surface methodology (RSM) analysis and evaluate the effects of extraction time, temperature, solvent ratio, and pH on the extract yield and crude pectin content. Based on RSM analysis, 4.35 h, 98.04 °C, 23.34 solvent ratio, and pH 3.36 are the optimal parameters for extracting crude pectin from red okra, and the crude pectin content was predicted to be 40.83%. When red okra was extracted under these extraction conditions (4 h, 100 °C, 23 solvent ratio, and pH 3), the extraction yield was 45.26%, and the crude pectin content was 38.42%, which was similar to the yield obtained under the conditions derived from the RSM analysis. In addition, the pH control using hydrochloric acid was replaced with citric acid, and the changes in extract yield and crude pectin content were compared. When citric acid was used, the extract yield was 49.15% (8.6% increase), and the crude pectin content was 42.76 ± 2.56% (11.3% increase); compared to when hydrochloric acid was used, the yield increased. Finally, the standardization of red okra raw materials was determined by analyzing the extraction yield and crude pectin content by part, harvest time, and size of red okra using the established extraction method using citric acid. As a result, it was confirmed that the extraction yield obtained from the established extraction method was reached from the extraction of red okra fruits up to 12 cm in size that were harvested between July and November. Additionally, compared to the non-pectin fraction, the crude pectin fraction isolated from red okra pod extract showed significantly higher total phenolic content (TPC) and total flavonoid content (TFC). These findings, reported for the first time, may contribute to the development of processes to purify red okra pectin, functional evaluation studies of pectin, and potential applications of red okra extract in various industries and research.

Comparison of Diuretic and Electrolyte Excretory Activity between Compounds of Okra Fruit (Abelmoschus esculentus) and Furosemide

Introduction: Hypertension takes place of being one of the most concerning health issues, leading to increasing rate of death due to its complications. Pharmacological therapy diuretic become one to intervene on hypertension’s pathological sequences through sodium and potassium regulation. Similar diuretic effect can be achieved through fruits and vegetables crop including Okra fruit (Abelmoschus esculentus). Objective: to investigate the diuretic variance of different part of Okra fruit’s (Abelmoschus esculentus). Methods: In total of 8 groups of male Rattus norvegicus rats are sorted into control and intervention groups. As control, the C- Group (negative control group) was given Na- CMC 0.5% suspension, and C+ Group (positive control group) was given furosemide. The experimental groups P1, P2, and P3 were given Okra (Abelmoschus esculentus) whole fruit ethanolic extract; P4, P5, and P6 Group are were given Okra (Abelmoschus esculentus) seedless fruit ethanolic extract within range concentration of 75-300 mg/kg body weight. Urine volume is measured every hour for 6 hours and the 24th hour. Sodium and potassium measurements are executed towards 24-hour urine accumulation. Results: Single dose administration of Okra (Abelmoschus esculentus) fruit significantly increase 5th hour urine volume as compared to furosemide (p < 0.001). Whole fruit of Okra (Abelmoschus esculentus) compared to seedless fruit exhibit no significant increment of urine volume and electrolytes (p > 0.05). Conclusion: Ethanolic extract of Okra (Abelmoschus esculentus) fruit produce similar diuretic effect to furosemide. However, seedless Okra (Abelmoschus esculentus) fruit exerts a higher kaliuretic effect compared to whole fruit and furosemide.

Lid scrubbing with a gel combining natural extracts for dry eye treatment.

To evaluate the effect of lid scrubbing using a gel combining different natural extracts on ocular signs and symptoms of dry eye patients. A retrospective study was performed on 19 dry eye patients (51.1±16.0y). Non-invasive tear break-up time, tear meniscus height, meibomian gland loss, lipid layer thickness, conjunctival redness, corneal staining, ocular surface disease index (OSDI), and ocular pain intensity were measured before (baseline), 1wk, and 2mo after lid scrubbing with a gel containing different natural extracts (okra extract, aloe vera leaf juice, hydrolysed soy protein, caffeine, citrus unshiu peel extract, and raspberry seed oil). Compared with the baseline, there was a statistically significant improvement in both first (P=0.048) and average (P=0.026) non-invasive tear break-up time 2mo after treatment, as well as in corneal staining (P=0.043, 0.012), OSDI (P<0.001), and ocular pain intensity (P<0.001) after 1wk and 2mo. In addition, there was no correlation between ocular signs and symptoms. The lid scrubbing with a gel combining different natural extracts show beneficial effects on tear film stability, corneal damage, and ocular symptoms, which is the reason why this therapeutic procedure is proposed as an alternative for dry eye management. However, it is not possible to attribute this beneficial effect solely to the presence of the natural extracts in the gel, primarily due to the absence of a negative control group.

Effect of Spraying with Salicylic Acid and Okra Plant Extract on the Growth and Yield of Baashiqi Olive Trees

This experiment was conducted at Ghabat Al-Nouriyeh station, which is a unit belonging to the Plant Production Department, Diwaniyah Directorate of Agriculture, for the agricultural season,2022, on olive trees Olea erupea L. Baashiqi cultivar, 15 years age.To know the effect of spraying salicylic acid in three concentrations (0, 75, 150 mg L−1) and okra extract in three concentrations (0, 5, 10 ml L−1) and the effect of the interaction between the two agents on the vegetative and fruiting traits of olive trees Baashiqi cultivar. The study was implemented as a factorial experiment with two factors according to the randomized complete block design (RCBD) and with three replicates of 27 trees, each tree being an experimental unit. The results of the study showed that the spraying treatments with salicylic acid achieved a significant effect on the following vegetative and fruiting growth traits (the rate of increase in the length of the main branch, the relative content of chlorophyll in the leaves, the weight and size of the fruit, and the percentage of oil), Spraying treatments with concentrations of okra extract achieved a significant effect on a number of vegetative and fruiting traits(the rate of increase in the length of the main branch, total carbohydrates in the branches, size and weight of the fruit, and the percentage of oil). While none of the two factors achieved a significant effect on the traits of leaf area.

Effects of okra (Abelmoschus esculentus L.) leaves, fruits and seeds extracts on European sea bass (Dicentrarchus labrax) leukocytes, and their cytotoxic, bactericidal and antioxidant properties.

Okra, Abelmoschus esculentus L., is a popular vegetable crop with many bioactive compounds. The in vitro immunostimulant, cytotoxic, bactericidal and antioxidant activities of ethanolic extracts obtained from different parts of okra (leaves, fruits, and seeds) were studied. Phytochemical screening of hydroalcoholic extracts of okra leaves, fruits and seeds revealed a significant content of total phenols and flavonoids. Notable effects on the activities of leukocytes in the head kidney of European sea bass (Dicentrarchus labrax) (viability, phagocytic ability and capacity, and respiratory burst), as well as on peroxidase leukocyte contents were detected after incubation for 24h with different concentrations (0.01-1mgmL-1) of the extracts. The mean concentrations (0.1 and 0.5mgmL-1) of the different extracts increased the phagocytic ability and respiratory activity of head kidney leukocytes. However, the mean concentrations (0.1mgmL-1) of leaf and fruit extracts significantly decreased the peroxidase activity of leukocytes. In addition, all ethanolic okra extracts at higher concentrations (1mgmL-1) produced a marked reduction in the viability of the DLB-1cell line compared to the viability recorded in the control samples. In addition, ethanolic extracts used at 0.5 and 1mgmL-1had a significant cytotoxic effect on the viability of PLHC-1cells. Finally, all doses of seed and leaf extracts at higher concentrations (0.5 and 1mgmL-1) showed significant bactericidal activity on two fish pathogenic bacteria, Vibrio anguillarum and V. harveyi strains. Finally, a remarkable antioxidant activity was detected on the ethanolic extracts. All these results point to their possible use as an alternative to chemical compounds in farmed fish.

Pengaruh Pemberian Ekstrak Okra (Abelmoschus Esculentus) terhadap Peningkatan Jumlah Eritrosit dan Kadar Hemoglobin (Hb) Darah Tikus Putih Jantan (Rattus Norvegicus) Anemia yang Diinduksi Natrium Nitrit (NaNO2)

Hemolytic anemia is a condition which the number of erythrocytes and hemoglobin levels are less than normal due to shortening of life span, rupture and destruction of erythrocytes. One of the factors causing the rupture and destruction of erythrocytes is oxidative stress. Okra contain flavonoid antioxidants that can counteract and prevent oxidative stress. This research to determine the effectiveness of okra (Abelmoschus Esculentus) extract on increasing the number of erythrocytes and hemoglobin (Hb) levels in the blood of anemia male white rats (Rattus Norvegicus) induced by sodium nitrite (NaNO2). Experiment with Posttest Only Control Group Design was held. The number of samples used were 24 male white rats (Rattus norvegicus) which were divided into 4 groups; the positive control group, which was only given NaNO2 and the other three groups were given NaNO2 and okra extract (Abelmoschus Esculentus) at 200mg/kgBW/day, 300mg/kgBW/day, and 400mg/kgBW/day for 28 days. Blood samples were taken and then the number of erythrocytes and hemoglobin levels were calculated using a hematoanalyzer. Anova test results obtained a significant value (sig, 0.000) between groups. Okra (Abelmoschus Esculentus) extract was able to increase the number of erythrocytes and hemoglobin (Hb) levels. Okra (Abelmoschus Esculentus) extract has an effect on increasing the number of erythrocytes and blood hemoglobin levels of anemic male white rats.

The Inhibition of Green Okra Fruit (Abelmoschus Esculentus) Extract Against Streptococcus Viridans Root Canals of Teeth

Background: Streptococcus viridans is the most frequently isolated bacterium from infected root canals (63%) and the leading cause of life-threatening sub-acute endocarditis (35%). The use of 2.5% NaOCl as a root canal irrigation material has disadvantages, it is a bad smell and taste, causing allergies, irritating and toxic, so other alternatives are needed. The green okra fruit extract has secondary antibacterial metabolites such as flavonoids, alkaloids, saponins, tannins, and terpenoids. Purpose: This study aims to determine the inhibition of green okra fruit extract against S. viridans. Methods: This research is an experimental laboratory in vitro with a post-test-only control group design. Antibacterial test using the disk diffusion method which consisted of 5 research groups (green okra extract with a concentration of 1,56%, 3,125%, 6,25%, 12,5%, and 2,5% NaOCl) and 4 repetitions. The result was analyzed using non-parametric statistical tests. Results: The average diameter of inhibition zone of each concentration of green okra fruit extract is 1,56% (0 mm ), 3,125% (0 mm), 6,25% (14,58 mm), 12,5% (18,19 mm), and 2,5% NaOCl (23,55 mm). The Kruskal-Wallis test showed a significance value of less than 0.05 which meant that there was a difference in inhibition in all groups. The Mann-Whitney test showed that there were significant differences between the study groups (α&lt;0,05), except for the green okra extract with a concentration of 1,56% with 3,125%. Conclusion: Green okra fruit extract (Abelmoschus esculentus) has inhibition against S. viridans at concentrations of 6,25% (14,58 mm) and 12,5% (18,19 mm) including the strong category.

Natural okra-based hydrogel for chronic diabetic wound healing

As a representative of chronic wounds, the long-term high levels of oxidative stress and blood sugar in chronic diabetic wounds lead to serious complications, making them the biggest challenge in the research on wound healing. Many edible natural biomaterials rich in terpenes, phenols, and flavonoids can act as efficient antioxidants. In this study, okra extract was selected as the main component of a wound dressing. The okra extracts obtained via different methods comprehensively maintained the bioactivity of multiple molecules. The robust antioxidant properties of okra significantly reduced intracellular reactive oxygen species production, thereby accelerating the wound healing process. The results showed that okra extracts and their hydrogel dressings increased cell migration, angiogenesis, and re-epithelization of the chronic wound area, considerably promoting wound remodeling in diabetic rats. Therefore, okra-based hydrogels are promising candidates for skin regeneration and wider tissue engineering applications.

Formulation and Evaluation of Moisture Protective Tablet Compression Coat Using Natural Polymers

This study aims to formulate and evaluate moisture protective coat for tablets using natural polymers of Fenugreek seeds and okra fruits in different concentrations compared with synthetic moisture protective coat. Ethanolic extracts of fenugreek and okra were treated with acetone as solvent for isolation of the mucilage, which is then dried and powders obtained identified and tested for polysaccharides using Molisch's reagent, Barofoead, KOH, Fehling reagent. The physicochemical tests including pH, viscosity, moisture content, percentage yield and swelling index were also carried out. Two formulations of 150 mg Silica gel tablets weighing 300mg and 500mg using PVP, magnesium stearate and Microcrystalline cellulose (MCC) as excipients were prepared and the powder mixture of flow ability was evaluated by measuring Carr's index, Hausener ratio and Angle of repose. The compressed tablets were evaluated according to USP monograph by testing the physical test of both tablets. Two coating materials were prepared and evaluated using natural polymers in different concentration 5%w/w and 10%w/w. The coated tablets with natural and synthetic polymers were compared by observing weight and moisture change of tablets pre and after stability study for 2 weeks in stress conditions at temperature (500 C) and RH 75 %. All the identification tests were positive indicating the presence of polysaccharides. Also, the physicochemical parameters tested were within the acceptable limits. Both fenugreek and okra mucilage's as polymers in low concentration 5%w/w revealed much better results regardless of weight of tablets. Coat defects were observed in tablets coated with 10%w/w of the polymers. The standard film coating polymer showed better moisture protection as moisture content about 4%. coating materials were successfully prepared and evaluated as moisture protective coat of silica gel tablets. Optimum coating formulations were found at 5%w/w concentration for mucilage's of both fenugreek and okra.