Oilseed Research Articles

Efecto del uso de boquillas de aire inducido y adyuvante sobre la calidad de aplicación de fitosanitarios

Spraying of phytosanitary products must ensure efficient pest control while avoiding losses that could damage human health and environment. The objective of the study was to evaluate the effect of conventional hollow cone and air-induced hydraulic nozzles, and the addition of an adjuvant (Methylated Seed Oil [MSO]) upon the application quality of a fungicide (penetration, distribution, and uniformity) and losses due to endodrift on wheat and soybean canopies. In each crop, the experimental design was completely randomized,with four replications and a factorial arrangement of nozzles x adjuvant. Droplet spectrum was evaluated by using water-sensitive cards, and brilliant blue as a tracer in the application mixture. According to the results, the MSO did not homogenize the distribution of droplets generated by air-induced nozzles in the wheat canopy, as it depended on the crop height evaluated. In addition, the MSO reduced endodrift independently of the type of nozzle used. Thedistribution of droplets on the soybean canopy depended on the height and not on the type of nozzle nor the addition of MSO. The MSO and its combination with the nozzle type did not reduce endodrift in this crop.

Assessment of Moroccan Cannabis Sativa Seed Oil: Chemical Analysis and Evaluation of Antioxidant, Toxicological, and Antinociceptive Effects.

Assessment of Moroccan Cannabis sativa Seed Oil: Chemical Analysis and Evaluation of Antioxidant, Toxicological, and Antinociceptive Effects. by K. Raoui et al., Cadi Ayyad University, Marrakech, Morocco. Cannabis sativa L., locally known as "El kif", belongs to the Cannabaceae family. This study aims to conduct a chemical analysis of Cannabis sativa seed oil (CSSO) and assess its acute toxicity, antioxidant properties, and analgesic effects. The chemical analysis was performed using gas chromatography and mass spectrometry (GC/MS) to identify fatty acids (FAs) contents. Antioxidant activity was evaluated in vitro using the (2,2-diphenyl-1-picrylhydrazyl) DPPH radical scavenging method and the (ferric reducing antioxidant power) FRAP method. Concurrently, acute toxicity, along with antinociceptive activity, was studied through three distinct animal models: writhing test, formalin test, and hot plate test. The results revealed that linoleic acid, oleic acid, α-linolenic acid, and palmitic acid were the main components of CSSO. The LD50 of CSSO was greater than 5 g/kg, indicating low toxicity. Additionally, CSSO exhibited a significant content of flavonoids and total polyphenols, along with notable antioxidant activity with important values. The results indicated a significant increase in thermal stimulus latency, a reduction in the number of writhes induced by acetic acid, and a decrease in licking time in both phases of the formalin test. In conclusion, this study suggests promising results for CSSO, emphasizing its potential as a therapeutic agent.

Production of biodiesel from Pistacia atlantica (PA) oil seeds using nano-catalyst of alumina/sludge of stone cutting factory (Al2O3/SSCF) and separation of biodiesel and glycerol phases by high voltage

Biodiesel is a renewable fuel and a suitable alternative to fossil fuels. However, the cost of its production and purification has prevented the commercialization and industrialization of this fuel. One economic solution for producing biodiesel fuel is the profit from the sale of glycerin, which is produced as a side product in various processes. In addition to the economic aspects, there are problems caused by the presence of glycerin residues in raw biodiesel before the purification process and the low speed of its separation from biodiesel. Biodiesel is produced by the transesterification method from Pistacia atlantica (PA) oil seeds of the Lorestan Mountains and methanol (1:6) using nano-catalysts of alumina/sludge from a stone cutting factory (Al2O3/SSCF). In this study, to investigate cost reduction and pure and quick separation of glycerin from biodiesel produced, a high-voltage electric method has been used. In this method, negative charges are absorbed by glycerin, separating it from biodiesel. The resulting biodiesel is produced in 10 min with 99% efficiency. The Fourier-transform infrared spectroscopy (FTIR) spectrum of biodiesel is investigated to ensure the formation of the reaction. This method can replace traditional methods.

Preparation of Nano-Lycopene from Tomato Waste and its Use in Extending the Shelf Life of Flax Seed Oil

Preparation of Nano-Lycopene from Tomato Waste and its Use in Extending the Shelf Life of Flax Seed Oil

The Protecting Role of Black Seed Oil and Its Nano-Formulation in LPS-Induced Acute Kidney Injury in Mice: Evaluation of Oxidative Stress, Biochemical & Molecular Parameters.

Acute kidney injury (AKI) is a medical concern that is accompanied by the rapid deterioration of kidney function. It can be triggered by lipopolysaccharide (LPS) of gram-negative bacteria as it activates a complicated immune response, resulting in widespread inflammation and potential organ dysfunction. Black seed oil (BSO) is rich in beneficial constituents and has been widely used owing to its nutritional advantages. This research is aimed to investigate the potential protective effects of BSO and its nano-formulation on AKI induced by LPS. It also aimed to compare their anti-inflammatory activity with indomethacin, a known synthetic anti-inflammatory drug. Forty-eight mice were placed randomly into 8 groups. A single intraperitoneal (i.p.) injection of 2.5 mg/kg B.W. of LPS was used to trigger inflammation, and pretreatment with BSO and its nano-formulation was at 0.2 mL/kg/day for 14 consecutive days. Indomethacin was used as a reference drug and its efficacy was tested alone or in combination with BSO at lower doses. Renal function was assessed using urea, creatinine, neutrophil gelatinase-associated lipocalin (NGAL) and kidney injury molecule-1 (KIM-1). Also, oxidative and inflammatory markers were assessed by measuring levels of reduced glutathione (GSH), nitric oxide (NO), cyclooxygenase-2 (COX-2), tumor necrosis factor alpha (TNF-α), and toll-like receptor-4 (TLR-4). Histopathological examination of the kidney tissues was also performed. The study showed that BSO and its nano-formulation had anti-inflammatory effects comparable to or better than those of indomethacin. They greatly decreased the oxidative stress and inflammatory markers induced by LPS. Their protective effect against pathological alterations in kidney tissues was significantly noticed. BSO and its nano-formulation could be used as nephroprotective and anti-inflammatory supplements.

Ameliorative Effect of Parsley and Pumpkin Seeds Oils against Cosplatin Induced Hepatorenal Injury in Male Albino Rats (Physiological and Histological Study)

Ameliorative Effect of Parsley and Pumpkin Seeds Oils against Cosplatin Induced Hepatorenal Injury in Male Albino Rats (Physiological and Histological Study)

The Effect of Adding Different Proportions of Local Moringa oleifera Seed Oil on the Shelf Life Extension of Flaxseed Oil

The Effect of Adding Different Proportions of Local Moringa oleifera Seed Oil on the Shelf Life Extension of Flaxseed Oil

Lipid classes and fatty acid composition of Thapsia garganica L. seeds oil

This study focused on characterizing the seed oil of Thapsia garganica (Apiaceae), a medicinal plant native to Laghouat, Algeria, and evaluating its antioxidant properties. Various solvent systems were employed to extract and fractionate the lipid content of T. garganica seeds oil. Chemical indices were determined, and fatty acids methyl esters were analyzed using GC/MS. Tocopherol composition was assessed via HPLC, and antioxidant activity was evaluated using the 2,2-diphényl 1-picrylhydrazyle (DPPH) method. The GC/MS analysis revealed distinct fatty acid profiles across various fractions, highlighting a notable presence of petroselinic acid and higher-than-usual levels of pentadecanoic acid in all fractions. T. garganica oil exhibited richness in tocopherols, particularly with α-tocopherol being the predominant homolog. The antioxidant activity assessment of different lipid fractions indicated potent activity within polar lipids (glycolipids and phospholipids). Furthermore, T. garganica oil was abundant in unsaturated fatty acids, notably petroselinic acid, displaying significant radical scavenging activity in its polar fractions. Keywords: T. garganica, petroselinic acid, pentadecanoic acid, tocopherols, antioxidant activity.

Assessment of Moringa Seed Oil: Fatty Acid Profile, Oxidative Stability and Antioxidant Activity

Moringa oleiferais called the “miracle tree” and it has more vitamins than even some fruits and vegetables like oranges, carrots etc. Objective: To assess functional groups and compare oxidative stability, fatty acid profile, free fatty acid concentration, and antioxidant activity to commercial vegetable oil. Methods: The moringa seed oil was extracted by cold press extraction and solvent extraction by n-hexane and petroleum ether. Functional group, lipid peroxidation, fatty acid profile, antioxidant activity, and FFAs % were analyzed by FTIR, TBARS, GC-MS, DPPH, and titration respectively. Results: The FTIR spectra of prepared samples showed common functional groups of triglycerides, including a sharp peak at 2984 cm-1 for aliphatic C-H stretching. The maximum TBARS value was 0.234 ± 0.03% in T0 at 30 days of storage, whereas the lowest was 0.167 ± 0.04% in T1 at 0 day. The GC-MS analysis of screw press moringa oil showed a high percentage of monounsaturated fatty acids, with 71.38 ± 0.01% oleic acid, 7.01 ± 0.01% palmitic acid, and 1.92 ± 0.01% linoleic acid. At 15 days of storage, FFAs were 2.28 ± 0.06%, showing low hydrolytic rancidity. The Antioxidant Activity in DPPH analysis was 44.46 ± 0.02%, showing high antioxidant properties. Conclusion: The characteristics of moringa seed oil indicate that it could be an effective edible oil and suitable for the production of food items and other edible products in the food and nutraceutical sectors.

Reduction of Emissions in a Single Cylinder Diesel Engine using Blends of Diesel & Cotton Seed Oil with Unconventional Catalytic Converter

This paper investigates the feasibility of mitigating emissions from diesel engines through the combined application of alternative fuels and an unconventional catalytic converter employing cost-effective catalysts. The dwindling reserves of fossil fuels necessitate the exploration of sustainable power sources for internal combustion engines, thereby reducing our dependence on these finite resources. This study evaluated the performance parameters and emissions of a single-cylinder, four-stroke, stationary diesel engine fueled with blends of 10%, 20%, and 30% cottonseed oil in diesel (by volume). Following the identification of the optimal blend, a performance test was conducted again with the inclusion of a custom-designed and fabricated catalytic converter. Exhaust emissions were subsequently measured with and without the converter in operation. The design of the unconventional catalytic converter considered the engine's specifications, incorporating cerium oxide and sponge iron as oxidation catalysts for CO and hydrocarbon (HC) conversion. Charcoal was employed as a reduction catalyst to specifically target NOx emissions.

Determination Of Aflatoxin in Various Spices Samples and Its Detoxification Using Black Seed Oil: A Biological Approach

Aflatoxins are poisonous compounds generated by specific fungal species that are naturally occurring everywhere and are essentially inevitable. They can seriously endanger human health by contaminating food crops. Aflatoxin contamination of spices is a serious worldwide problem that affects trade and they are cited as the first significant risk in border rejection. Objectives: The objectives of this study were to ascertain the aflatoxin content of different spices samples and to use varying concentrations of black seed oil to detoxify positive samples. Methods: Thin Layer Chromatography (TLC) was used to determine the aflatoxins in various spices and contaminated sample were detoxified by black seed oil. Results: From this study aflatoxins were detected in 70% and 30% spices samples have no aflatoxin. Among contaminated samples 43% had aflatoxins beyond the permissible limits whereas 57% had the aflatoxins below the permissible limits. The positive samples were alleviated by biological method i.e. black seed oil (1-10%) which detoxified aflatoxin in fennel 49.52 ± 1.50 – 92.50 ± 1.94% and detoxification level was found in cumin seed 55.37 ± 1.52 – 87.32 ± 1.83 while in fenugreek it was ranging from 50.20 ± 1.50 – 82.37 ± 1.75. Conclusions: This study showed that black seed lowered aflatoxin levels in some spices.

Application of Aspen Plus to Modelling and Simulation of Neem Seed Oil Extraction

The demand for neem oil keeps on increasing due to its wide applications in pharmaceutical, agro–allied industries, cosmetics and soap production. To avoid wastage of material and safety of operation and personnel, the designed plant must be simulated to predict its performance before actual construction. The property method based on the non–random two–liquid (NRTL) model of Aspen Plus V12 was used to simulate the designed plant and its predicted performances were 87.13% and 99.60% for quantity of neem oil to be obtained from the evaporator and quantity of solvent (ethanol) recovery from the condenser as against 85.93% and 94.93% of the practical result with 1.38% and 4.69% errors respectively. The extracted oil has iodine, acid and saponification values of 60.10 g I2/100 g, 4.2 mg KOH/g and 210.0 mg KOH/g respectively. The fatty acid compositions of oil are oleic acid, 46.61%; stearic acid, 11.83%; palmitic acid, 16.54%; 11 – octadecenoic acid, 3.58%; cis–vaccenic acid, 5.90%; cyclopropaneoctanal, 11.19%; squalene, 0.21% and trimethylsilyl–di(timethylsiloxy)-silane, 4.14%. Based on the quality and fatty acid composition of the extracted neem oil, it is highly recommended for cosmetics and soap production.

Production of Rhamnolipids using WFSO and its application in the development of antifungal nanoemulsion using Hydnocarpus Wightiana, Garcinia Cambogia - Seed oils

Presently, Industrial-scale demands for manufacturing rhamnolipids (RhLs) are not so developed; probably due to the high cost of the substrates and the rare demand for costlier surfactants. Therefore, the use of waste frying oils (WFSO) as substrates in RhLs manufacturing using Pseudomonas aeruginosa could be a notable substitute for the regularly used sources of oils and fatty acids to minimize manufacturing charges. WFSO as a byproduct normally consists of Linoleic acid as a prime fatty acid constituent. In the present work, P. aeruginosa yielded RhLs at an uttermost concentration of 7.4 g L−1; while constructional characteristics proved the presence majority of di-RhLs in the mixture. Also, on testing, the SFT (Surface Tension) reduced to 34.6±0.1 mN m−1; and an interfacial tension against heptane went down to 5.2±0.05 mN m−1. The oils from kernels of Hydnocarpus Wightiana and Garcinia cambogia were extracted separately; further, the detailed analysis showed the presence of their corresponding active fatty acids. In the initial screening of antifungal efficacy against Fusarium oxysporum (fusarium wilt) and Diplodia maydis (Diplodia ear and stalk rot of maize), RhLs shown the minimum inhibitory concentration (MIC) between 250 and 500 ppm, both extracted oils shown the MIC between 5 % and 10 %. Therefore, we decided to prepare the nanoemulsions (NEs) using 0.5 % RhLs, and 10 % of each respective extracted oil; which upon analysis showed the globule size as low as 110.9 nm and 147.0 nm respectively. The turbiscan study revealed that NEs made using RhLs as an adjuvant were found more stable than the alkyl polyglucosides, and the nanoemulsions containing RhLs showed higher levels of antifungal activity against F. oxysporum and D. maydis in the antifungal study. Thus, a successful utilization of the WFSO for the production of RhLs; and reduction in the probable water or soil pollution was achieved, as well as our RhLs bio-synthesis cost was much less as compared to the existing manufacturing process.

Sesamum Indicum L. Seed Oil Synergistically Enhances The Effectiveness of Acetylcholine Esterase Inhibitor in Managing Scopolamine-Induced Neurological Dysfunction in Rats

Sesamum Indicum L. Seed Oil Synergistically Enhances The Effectiveness of Acetylcholine Esterase Inhibitor in Managing Scopolamine-Induced Neurological Dysfunction in Rats

Copperized Karanj Seed (Pongamia pinnata) Oil as an Alternative to Chemical Preservative in Plywood Manufacturing

Most chemicals used in wood preservation are toxic to the environment. Use of synthetic preservatives are highly prevalent in plywood manufacture. Wood, which is the basic raw material for plywood manufacture, being ligno-cellulosic material is liable to degradation due to microbiological organisms and other biological wood destroying organisms causing significant loss. Therefore, it is necessary to develop an alternate naturally available preservative which will be effective for plywood manufacture. Karanj or Pongamia pinnata is known for its anti-fungal and pesticide properties that make it an ideal material as wood preservative. Therefore, an attempt was made to develop an eco-friendly green preservative using naturally available plant resources with high toxicity to wood destroying organisms and low toxicity to humans. Copper was incorporated into Karanj seed extractive and 12mm thick plywood samples were prepared at different concentrations 0.5% to 5% of preservative which were subjected to tests for efficacy study and mechanical properties as per Indian Standards. From the results obtained in this study, it can be concluded that 3% concentration of the preservative can be utilized in plywood manufacture which can resist against wood borers and termites. Concentration above 3% of the preservative interfere with the bonding properties of the resin and significantly reduces glue shear strength.

Camelina sativa Seeds and Oil as Ingredients in Model Muffins in Order to Enhance Their Health-Promoting Value.

The aim of this study was to see whether it is possible to add camelina oil and seeds as ingredients in muffins in order to enhance their health-promoting value, such as their bioactive compound content, while maintaining the organoleptic attributes considered desirable by consumers. Camelina oil is characterised by a high linolenic acid content. Four types of muffins were prepared for analysis: MBnO-control muffins (containing 11.85% rapeseed oil), MCsO-muffins containing camelina oil instead of rapeseed oil, MCsS-muffins containing 6.65% camelina seeds in relation to the mass of prepared dough, and MCsOS-muffins containing both camelina oil and camelina seeds. The change in the fatty acid profile in muffins with the addition of camelina oil was significant; however, it was found that, as a result of thermal treatment, lower amounts of saturated fatty acids were formed. Among all the investigated experimental variants, muffins were characterised by the highest contents of all the phenolic acids analysed. The substitution of rapeseed oil with camelina oil had no negative effect on most of the organoleptic attributes of the muffins. Moreover, thanks to a greater content of carotenoids, camelina oil had an advantageous effect on the improvement of product colour, thus improving its overall desirability.

Functional Characterization of the Effects of CsDGAT1 and CsDGAT2 on Fatty Acid Composition in Camelina sativa.

Triacylglycerols (TAGs) are the storage oils of plant seeds, and these lipids provide energy for seed germination and valuable oils for human consumption. Three diacylglycerol acyltransferases (DGAT1, DGAT2, and DGAT3) and phospholipid:diacylglycerol acyltransferases participate in the biosynthesis of TAGs. DGAT1 and DGAT2 participate in the biosynthesis of TAGs through the endoplasmic reticulum (ER) pathway. In this study, we functionally characterized CsDGAT1 and CsDGAT2 from camelina (Camelina sativa). Green fluorescent protein-fused CsDGAT1 and CsDGAT2 localized to the ER when transiently expressed in Nicotiana benthamiana leaves. To generate Csdgat1 and Csdgat2 mutants using the CRISPR/Cas9 system, camelina was transformed with a binary vector carrying Cas9 and the respective guide RNAs targeting CsDGAT1s and CsDGAT2s via the Agrobacterium-mediated floral dip method. The EDD1 lines had missense and nonsense mutations in the CsDGAT1 homoeologs, suggesting that they retained some CsDGAT1 function, and their seeds showed decreased eicosaenoic acid (C20:1) contents and increased C18:3 contents compared to the wild type (WT). The EDD2 lines had a complete knockout of all CsDGAT2 homoeologs and a slightly decreased C18:3 content compared to the WT. In conclusion, CsDGAT1 and CsDGAT2 have a small influence on the seed oil content and have an acyl preference for C20:1 and C18:3, respectively. This finding can be applied to develop oilseed plants containing high omega-3 fatty acids or high oleic acid.

Studying the effectiveness of Jatropha carcus L. Extract as a repellent, antifeedant, and toxic substance against red palm weevil (Rhynchophorus Ferrugineus) adult insects in Saudi Arabia

ObjectiveRed palm weevil (RPW), Rhynchophorus ferrugineus, is a highly harmful pest that causes severe damage to date palm trees. The detrimental influence of RPW on date palm trees cannot be ignored and must be addressed. Chemical insecticides are available for controlling RPW; however, the insect population has become resistant to these chemicals, leading to damage and spread to new areas. The present investigation examined the impacts of physic nut (Jatropha curcas L.) seeds essential oils (PNEO) on laboratory-reared RPWs adult insects reared in a controlled laboratory environment. MethodsThe insecticidal, repellent, and antifeedant potentials of different doses (10, 20, 30, 40, 50%) of PNEO were tested against RPW. The insecticidal activity of J. curcas seeds oil extracts was assessed using topical application and feeding methods. ResultsRPW showed a high mortality rate when J. curcas seeds oil was applied topically after 24 h (P < 0.05). The mortality (%) of insects was 8, 76, 92, 96, and 100 for 10 %, 20 %, 30 %, 40 %, and 50 % concentrations, respectively. The LC50 and LC90 were 16.50 %, and 28.14 %, respectively. Results from the feeding method demonstrated a significant increase in mortality with a 100 % mortality rate at higher concentrations of PNEO compared to 10 and 20 % concentrations (P < 0.0001). The LC50 and LC90 were 24.42 % and 31.30 %, respectively. There was no significant decrease in food consumption and the antifeedant index when 10 % and 20 % of J. curcas seeds oil were used on the 3rd and 6th day after application. All J. curcas oil concentrations showed a repellent effect significantly higher than the controls (P < 0.05). ConclusionJ. curcas seeds oil has shown potential insecticidal and repellent effects against RPW, and it could provide an eco-friendly tool for RPW pest management.

Production of Rubber Seed Oil: Study of Parameters and some Food Oil Properties

Rubber seeds are one of the plantation commodities that are underutilized by the Indonesian people, apart from being the generative seeds of rubber trees. Rubber seeds have a reasonably high vegetable oil content of 68.53% with a carbohydrate content of 6.99%, protein content of 17.41%, and ash content of 3.08%. This study aimed to obtain food oil from rubber seeds with fewer chemicals and more affordable production costs. This study investigates the properties of food oil from rubber seeds and analyzes the cyanide content. The oil extracted using the traditional method yielded an 8.48% oil content. The rubber seeds were soaked for four days with rice husk charcoal (1:1) to reduce cyanide levels, followed by cooking to obtain the oil and bleaching with bentonite for purification. The oil was characterized according to Indonesian National Standards (SNI), assessing cyanide content, organoleptic properties, water content, acid values, and peroxide numbers. The resulting oil had a density of 0.87 g/cm³ and a cyanide content of 0.54 ppm. The tests showed a water content of 0.04%, an acid value of 0.4 mg NaOH/g, and organoleptic properties that met SNI standards. However, the peroxide value of 13.5 meq O2/kg was slightly above the standard, making the oil non-edible.

Mitigating Effect of Lepidium sativum Seeds Oil on Ovarian Oxidative Stress, DNA Abnormality and Hormonal Disturbances Induced by Acrylamide in Rats.

Acrylamide (ACR), an industrial compound, causes both male and female reproductive toxicity. Lepidium sativum seeds (L. sativum) (Garden cress) are known for their health benefits as antioxidant, antiasthmatic, anticoagulant, anti-inflammatory, and analgesic agents. Therefore, this study aimed to investigate the phytochemistry and nutritional value of L. sativum seeds oil for attenuating the ovarian damage induced by acrylamide in rats. The phytochemical investigation of the seeds revealed the presence of vitamins, potassium, iron, sugar and amino acids. Twenty eight compounds from the unsaponifiable fraction and twenty three compounds from the saponifiable fraction were identified. Three sterols and two triterpenes were isolated and identified as β-sitosterol (1), ▵5-avenasterol (2), friedelanol (3), stigmasta-4, 22-dien-3-one (4), and ursolic acid (5). Treatment of acrylamide-induced rats with L. sativum seeds oil ameliorated prolactin (PRL), progesterone (P4), estradiol (E2), malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH), nitric oxide (NO), interleukin-6 (IL-6), and tumor necrosis factor alpha (TNF- α) with variable degrees. The histopathological findings of ovaries supported these results. In conclusion, compounds (3-5) were isolated for the first time from L. sativum seeds oil. The seeds oil attenuated the ovarian damage and could potentially be a new supplemental agent against female infertility.