Soil System Research Articles

Oil/Water Biphasic Solvent System for the Eco-Extraction and Cosmetic Formulation of Bixa orellana L.

Annatto, obtained from the seeds of achiote (Bixa orellana L.), is a widely used orange pigment rich in bixin and other apocarotenoids. This work reports the optimisation of a green extraction method of pigments and antioxidant compounds from achiote as well as its integration in a one-step green extraction-cosmetic formulation process. A biphasic solvent system of water and oil was used to recover simultaneously polar polyphenols, and less polar compounds, such as δ-tocotrienol and bixin. The optimisation of the ultrasound assisted extraction is presented, as well as a comparison of different vegetable oils used as extraction solvents. The composition, physicochemical properties and antioxidant activity of the oils were studied and their extraction performance was compared. Refined sunflower oil proved to be a better solvent than virgin olive, jojoba, coconut and grapeseed oils. Both aqueous and oil phases displayed an interesting antioxidant capacity. The oil phase contained 0.9% of bixin, as well as minor apocarotenoids and δ-tocotrienol. Twelve compounds, mainly phenolics, were identified by UHPLC-DAD-HRMS/MS in the aqueous phase. Twenty-one volatile compounds were identified in the volatile fraction by SPME-GC-MS. Lastly, a one-step green process is proposed to combine the extraction and the cosmetic formulation of the bioactive compounds.

Mechanism of Iron Transport in the Triticum aestivum L.–Soil System: Perception from a Pot Experiment

Iron is one of the necessary trace elements for plant growth and the human body. The ‘hidden hunger’ phenomenon in the human body caused by an imbalance of iron in soil is increasingly prominent. Addressing this issue and optimizing soil through regulatory measures to improve the absorption and utilization of iron by crops has become an urgent priority in agricultural development. This study carries out pot experiments to observe the growth process of Triticum aestivum L. under various soil iron environments. Combined with previous research results, the transport mechanism of iron in the soil—Triticum aestivum L. system was systematically explored. The results indicate that during the jointing and maturity stages of Triticum aestivum L., iron was preferentially enriched in the underground parts; at the maturity stage, the iron content in various organs of Triticum aestivum L. shows a trend of increase followed by a decrease with the soil iron content varying in the following sequence: deficient, moderately deficient, medium, moderately adequate, and adequate. The iron-deficient stress environment causes an increase in the effectiveness of rhizosphere iron, resulting in a higher level of iron in the plant stems, leaves, and seeds. Conversely, when the soil iron content is medium or adequate, the effectiveness of rhizosphere iron decreases, leading to a reduction in the iron content in each part of the plant. A concentration gradient of 7.2 mg/kg in the experimental setup is found to be the most favorable to the enrichment of iron in the shoots of Triticum aestivum L. plants. The findings of this experiment provide guidance for the fertilization strategy to mitigate iron deficiency symptoms in plants under similar acidic-alkaline conditions of soil, as well as a systematic mechanism reference and basis for studying the soil-plant-human health relationship.

A non‐ionic green surfactant extracted from the Anabasis setifera plant for improving bulk properties of CO2‐foam in the process of enhanced oil recovery from carbonate reservoirs

AbstractFoam, as a gas‐in‐liquid colloid, has a higher appearance viscosity than the one of both gas and liquid that form it. Adjusting the mobility ratio of the injected fluid–oil system and increasing gas diffusion in the foam injection process increase oil production. With these properties, foam as an injection fluid in fractured reservoirs has a major effect on oil production from the matrixes and prevents premature production of injection fluid. Surfactants are common foaming agents in injection water. Saponins are known as plant‐derived surfactants for forming stable foam. This feature, along with its cheap price and availability, can make them candidates for enhanced oil recovery (EOR) by the foam injection method. However, the utilization of CO2 as the gaseous phase in foam introduces additional machanisms of CO2 injection to the oil recovery operations. In this assessment, a non‐ionic green surfactant derived from the Anabasis setifera plant was used as a foaming agent, while CO2 served as the gas phase. A series of surface tension tests in CO2 environment were performed to determine the optimal concentration of the surfactant. Foaming tests were performed by a designed foam generator. The produced CO2‐foam was then injected into a fractured carbonate plug with six matrixes (with one horizontal and two vertical fractures). Based on the results, the water–CO2 surface tension was reduced to 20.549 mN/m. The optimum salinity based on the foam stability was 10,000 ppm. The half‐life of the foam was determined to be 40 min. Also, the foam characterization showed that the foamability of the surfactant was favourable for increasing oil production so that by secondary flooding, an oil recovery of more than 66% was achieved from the fractured carbonate plug.

Аккумулирование тепловой энергии масла и охлаждающей жидкости дизельного двигателя маневрового тепловоза

Objective: to substantiate the effectiveness of using heat-storing materials to preserve the thermal energy of the oil and coolant of the diesel power plant of a shunting diesel locomotive. To achieve this goal it is necessary: to identify the relevance of the problem of preserving the thermal energy of oil and coolant during the operation of diesel locomotives in conditions of negative ambient temperatures; develop a concept for the use of heat-storing phase transition materials in the oil and water systems of a diesel locomotive; carry out the selection of heat-storing materials based on their physical properties and the operating characteristics of diesel locomotive engine systems; conduct laboratory experimental studies to assess the effectiveness of using a particular heat-storing material. Methods: physical experiment performed in laboratory conditions, which consists of recording the temperature of the working medium (oil and water) and the temperature of the heat-storing material used depending on the cold idle time. Experimental studies make it possible to more accurately select the most preferred materials for accumulating heat from oil and water in diesel locomotive engine systems. Results: an experimental dependence of the temperature of various heat-accumulating materials on the cold idle time was constructed, which makes it possible to justify the use of stearin to accumulate heat from engine oil and cooling system water. Practical importance: the research was carried out as part of the implementation of a grant from Russian Railways JSC for young scientists to conduct scientific research aimed at creating new equipment and technologies for use in railway transport and recommended for use in the locomotive complex. Grant agreement No. 5103675 dated December 26, 2022 “Thermal accumulator for the pre-start preparation system for diesel locomotives in the cold season”

Enzymatic Diagnostics of Soil Health of the European Part of Russia with Lead Contamination

Lead (Pb) is one of the most common environmental pollutants. Lead has an acute toxic effect on soil biotas and the enzymatic system of soils. The objective of this study is to carry out enzymatic diagnostics of soil health in the European part of Russia after Pb contamination. As a part of the simulation experiment, Pb (at maximum permissible concentrations (MPCs) of 1, 10, and 100) was used to contaminate 12 types of soils in the south and center of the European part of Russia, which differed in their physical and chemical properties. To assess soil health, the activity of oxidoreductases (catalase, dehydrogenases, and cysteine reductase) and hydrolases (invertase, urease, and phosphatase) was studied. Most enzymes were inhibited with increased Pb dosage. The most sensitive soils to Pb contamination, assessed by enzyme activity, are soils of semi-deserts and dry steppes. Cysteine reductase is considered the most sensitive enzyme to Pb contamination. The most informative indicators for Pb contamination were phosphatase, cysteine reductase, and invertase. The P (phosphatase) cycle and the redox enzyme (catalase) also have instability in Pb-contaminated soils. The C (invertase and dehydrogenases) and N (urease) cycles do not change significantly when contaminated with lead. The results of this study can be used for the diagnostics of the condition of soils in different natural areas after Pb contamination.

Hexavalent chromium transformation during transport of L − cysteine modified nano − zero − valent iron supported by biochar via green synthesis in water and soil systems

Hexavalent chromium transformation during transport of L − cysteine modified nano − zero − valent iron supported by biochar via green synthesis in water and soil systems

Raspberry ketone loaded self nanoemulsifying system of cardamom oil: Assessment of phase behavior, dissolution rate and anti-hyperlipidemic activity

Raspberry ketone (RK), a polyphenolic antioxidant, possesses free radical scavenging against oxidative species and has potential of anti-inflammatory, anti-proliferative and anti-aging activities. Major pharmaceutical restriction associated with RK is its poor dissolution characteristic owing to its hydrophobic nature. Present investigation is an attempt to improve dissolution behavior of RK using cardamom oil (CO) based self nanoemulsifying system (SNE) design and preclinical assessed for anti-hyperlipidemic activity. Demarcation of phase boundaries to exactly locate nanoemulsion areas were explored using ternary plots drawn between CO as oil phase, Tween 80/PEG 600 as Smix at 1:1; 1:2; 2:1 and 3:1 ratios. Previously ternary components were selected on the basis of solubility of RK and aqueous dispersibility of CO. Nanoemulsion areas developed under ternary phase region were quantified using trapezoidal method and found in the order 1:1 > 1:2 > 3:1 > 2:1 of Smix. Aqueous dilutibility of SNEs followed the pattern of dilutions lines drawn across the ternary phase and its interference. Optimized SNE produced 97.1 % transmittance with average droplet size 101 nm, zeta potential −2.27 mV and 0.186 mS/cm conductivity. In-vitro RK release was examined in HCl buffer at pH 1.2 and phosphate buffer at pH 6.8 showed a significant difference in the RK dissolved (p < 0.01) compared to RK suspension (control F6). Optimized formulation (F3) at two doses level (RK loading of 80 and 160 mg/kg) vs. RK suspension (160 mg/kg) was pre-clinically administered for four weeks to assess anti-hyperlipidemic activity. Lipid profile markers (levels of VLDL, HDL, LDL, total triglyceride and total cholesterol) were significantly reduced from nanoemulsion formulation as compared to its suspension. SNE formulations were stable with no appreciable loss in quality and performance for six month storage at 30 ± 2 °C; 65 ± 5 % RH conditions. CO based SNE design significantly extended the ternary area for nanoemulsion development as well as RK solubility; which in turn improved dissolution rate of RK and showed anti-hyperlipidemic activity.

Functionality of wheat straw-derived biochar enhanced its efficiency for actively capping Cd and Pb in contaminated water and soil matrices: Insights through batch adsorption and flow-through experiments

The impact of functionality of biochar on pressing environmental issue of cadmium (Cd) and lead (Pb) co-contamination in simultaneous soil and water systems has not sufficiently reported. This study investigated the impact of Fe- and Mg-functionalized wheat straw biochar (Fe-WSBC and Mg-WSBC) on Cd and Pb adsorption/immobilization through batch sorption and column leaching trials. Importantly, Fe-WSBC was more effective in adsorbing Cd and Pb (82.84 and 111.24 mg g−1), regeneration ability (removal efficiency 94.32 and 92.365), and competitive ability under competing cations (83.15 and 84.36%) compared to other materials (WSBC and Mg-WSBC). The practical feasibility of Fe-WSBC for spiked river water verified the 92.57% removal of Cd and 85.73% for Pb in 50 mg L−1 and 100 mg L−1 contamination, respectively. Besides, the leaching of Cd and Pb with Fe-WSBC under flow-through conditions was lowered to (0.326 and 17.62 mg L−1), respectively as compared to control (CK) (0.836 and 40.40 mg L−1). In short, this study presents the applicable approach for simultaneous remediation of contaminated water and soil matrices, offering insights into environmentally friendly green remediation strategies for heavy metals co-contaminated matrices.

Fracture stratigraphy in oil-rich shale of the Upper Xiaganchaigou Formation (upper Eocene), western Qaidam Basin

Natural fractures in wells from oil-rich shale of the Upper Xiaganchaigou Formation in Qaidam Basin, the largest Cenozoic sedimentary basin in the Tibetan Plateau, vary systematically by stratigraphic position, allowing reconstruction of fracture generation processes during regional tectonic deformation. Compacted fractures, layer-bounded fractures, and low-angle (or bed-parallel) fractures were widely identified in oil-rich shale cores obtained from vertical wells. The layer-bounded fractures can be subdivided into two sets: one prevalent in light-colored (low gamma-ray) stiff layers and arrested by dark-colored (high gamma-ray) compliant layers, the other set primarily confined to the dark-colored compliant layers. The strikes of layer-bounded fractures, revealed by horizontal well FMI logs, are dominantly N60°-80°E and N10°-30°E within the light and dark intervals respectively. These strikes are consistent with natural hydraulic fracturing of the light layers during the Oligocene and the dark layers during the Middle Miocene-Holocene, based on a regional anticlockwise rotation of the compressional strain orientation. The abutment of layer-bounded fractures against low-angle (or bed-parallel) fractures in dark layers implies that the low-angle fractures were hydraulically fractured under a vertical least compressive stress during the Early Miocene, which produced the most intense tectonic compression. Layer-bounded fractures generally remain unmineralized in the dark layers but were mineralized in the light layers, suggesting that fractures confined in light layers would not decrease the sealing capacity of the shale oil system and fractures confined in dark layers can increase the pore space of shale oil storage. The Oligocene and Early Miocene fractures represented mechanical flaws that affected the initiation of Middle Miocene-Holocene fractures, implying that all natural fractures may alter hydraulic fracture stimulations to some extent.

Biosynthesized Selenium Nanoparticles as an Effective Tool to Combat Soil Metal Stresses in Rice (Oryza sativa L.).

Nanotechnology has demonstrated significant potential to improve agricultural production and increase crop tolerance to abiotic stress including exposure to heavy metals. The present study investigated the mechanisms by which aloe vera extract gel-biosynthesized (AVGE) selenium nanoparticles (Se NPs) alleviated cadmium (Cd)-induced toxicity to rice (Oryza sativa L.). AVGE Se NPs, chemically synthesized bare Se NPs, and NaSeO3 as an ionic control were applied to Cd-stressed rice seedlings via root exposure in both hydroponic and soil systems. Upon exposure to AVGE Se NPs at 15 mg Se/L, the fresh root biomass was significantly increased by 100.7% and 19.5% as compared to Cd control and conventional bare Se NPs. Transcriptional analyses highlighted that AVGE Se NPs activated stress signaling and defense related pathways, including glutathione metabolism, phenylpropanoid biosynthesis and plant hormone signal transduction. Specifically, exposure to AVGE Se NPs upregulated the expression of genes associated with the gibberellic acid (GA) biosynthesis by and 4.79- and 3.29-fold as compared to the Cd-alone treatment and the untreated control, respectively. Importantly, AVGE Se NPs restored the composition of the endophyte community and recruit of beneficial species under Cd exposure; the relative abundance of Azospirillum was significantly increased in roots, shoots, and the rhizosphere soil by 0.73-, 4.58- and 0.37-fold, respectively, relative to the Cd-alone treatment. Collectively, these findings highlight the significant potential of AVGE Se NPs to enhance plant growth and to minimize the Cd-induced toxicity in rice and provide a promising nanoenabled strategy to enhance food safety upon crop cultivation in contaminated agricultural soils.

Colonization ability and uniformity of resources and environmental factors determine biological homogenization of soil protists in human land-use systems.

Humans have substantially transformed the global land surface, resulting in the decline in variation in biotic communities across scales, a phenomenon known as "biological homogenization." However, different biota are affected by biological homogenization to varying degrees, but this variation and the underlying mechanisms remain little studied, particularly in soil systems. To address this topic, we used metabarcoding to investigate the biogeography of soil protists and their prey/hosts (prokaryotes, fungi, and meso- and macrofauna) in three human land-use ecosystem types (farmlands, residential areas, and parks) and natural forest ecosystems across subtropical and temperate regions in China. Our results showed that the degree of community homogenization largely differed between taxa and functional groups of soil protists, and was strongly and positively linked to their colonization ability of human land-use systems. Removal analysis showed that the introduction of widespread, generalist taxa (OTUs, operational taxonomic units) rather than the loss of narrow-ranged, specialist OTUs was the major cause of biological homogenization. This increase in generalist OTUs seemingly alleviated the negative impact of land use on specialist taxa, but carried the risk of losing functional diversity. Finally, homogenization of prey/host biota and environmental conditions were also important drivers of biological homogenization in human land-use systems, with their importance being more pronounced in phagotrophic than parasitic and phototrophic protists. Overall, our study showed that the variation in biological homogenization strongly depends on the colonization ability of taxa in human land-use systems, but is also affected by the homogenization of resources and environmental conditions. Importantly, biological homogenization is not the major cause of the decline in the diversity of soil protists, and conservation and study efforts should target at taxa highly sensitive to local extinction, such as parasites.

The effects of Zn, FYM and their combine application on soil physical and biological parameters under long-term rice based cropping systems in calcareous soil

The effects of Zn, FYM and their combine application on soil physical and biological parameters under long-term rice based cropping systems in calcareous soil

Design of Delivery Systems (Nanoemulsions and Biopolymer Nanoparticles) of Cloves Essential Oil: Preparation, Characterizations, Study the Release, and Antioxidant Activity

Abstract BACKGROUND: Clove oil has a wide range of therapeutic and pharmaceutical applications. It is also used in the manufacture of pesticides and antifungals, as well as many advantages. The study aims to improve the properties of essential oils, especially clove oil, and make them more stable by the design of delivery systems (nanoemulsions and biopolymer nanoparticles). METHODS: Clove oil was mixed with water in the presence of tween-20 as a polysorbate-type nonionic surfactant, and then carboxymethyl cellulose was added with different ratios of glycerin as a cross-linking agent. The formation of the clove nanogel was tested by spectrophotometric assay 2.2.2 Fourier transform infrared spectroscopy (FT-IR), Proton Nuclear Magnetic Resonance Spectroscopy (1H-NMR), dynamic light scattering (DLS), and morphology method field emission scanning electron microscopy (FESEM), also the rate of clove oil release during different periods, and hemolytic and antioxidant activity were tested. RESULTS: The nanoparticles were demonstrated by DLS and FESEM. The polydispersity index value was 0.279, indicating good monodispersity and good stability, while higher release values were obtained when glycerol was not present as a cross-linker. In addition to the higher antioxidant, values of the clove oil nano emulsion (CNE) have a maximum inhibition of 65% at a concentration of 100 μg/mL, whereas clove nano-gel (CNG) at the same concentration exhibited 59.2% inhibition. The calculated IC50 values of CNG and CNE were 6.58 and 3.25 μg/mL, respectively. CONCLUSION: The study proved through the results obtained that nanocomposites help stabilize components, especially volatile oil, which increases their effectiveness.

A modified cell-to-cell simulation model to predict oil-gas minimum miscibility pressure

Calculating the minimum miscibility pressure (MMP) between crude oil and carbon dioxide (CO2) is critical for optimizing injection parameters, designing schemes, and predicting production capacity in CO2 injection projects for enhancing oil recovery. However, an accurate approach for obtaining this parameter is not yet established. In order to tackle this issue, a novel approach is suggested, based on the original cell-to-cell model, to determine the MMP and the 97% oil recovery rate as the standard. Using the volume-transformed Peng-Robinson equation of state enhances the precision of fluid volume estimation, as it mainly relies on predicting fluid volume within each cell. Furthermore, to ensure a precise estimation of the ultimate oil recovery rate, it is imperative to employ a total cell count of 500 in all simulations to avoid the problem of numerical dispersion. Finally, a second-order polynomial equation more accurately predicts the infinite-cell oil recovery factor. The accuracy of the modified model is verified by comparing MMP values from five oil and gas systems in the literature. The computational results of the modified multiple-mixing-cell (MMC) approach exhibit a higher level of concordance with the MMPs in the literature. The average relative error is less than 3.96%. The improved MMC algorithm can quickly determine the miscibility mechanism and visually represent the dynamic miscibility process involving multiple oil-gas contacts in a slim tube. This study provides a theoretical and practical basis for addressing the critical scientific issues of CO2-safe storage technology.

Soil legislation and policies: Bibliometric analysis, systematic review and quantitative approaches with an emphasis on the specific cases of the European Union and Portugal

The literature shows that there are dimensions related to soil legislation and policy in the European Union contexts that can be better explored through bibliometric analysis, systematic reviews and quantitative approaches. Therefore, this research aims to analyse documents on soil legislation and policies, highlighting the specific cases of Portugal and the European Union (EU). The aim is to identify suggestions to improve the Portuguese and European Union soil policy instruments and measures. To achieve these objectives, a bibliometric analysis (considering text and bibliographic data) and systematic review were carried out, as well as a survey of the available soil legislation (considering qualitative data and quantitative analysis). The results show that soil legislation and policy have become more relevant in recent years and that concerns are about soil health, protection and safety, as well as risk mitigation, biodiversity preservation and the maintenance of ecosystem services. However, some topics could be further explored in future research, namely those related to multidisciplinarity, smart methodologies, soil salinisation, innovation and quantitative approaches to assessing policy impacts. This study presents suggestions that can be considered by the Portuguese and European Union policymakers to improve the respective soil legislation and policies. Defining a regulatory system for soils in the European Union has not been easy over time, although there have been attempts, given the specificities of the contexts related to soils and the reluctance of some member states to take certain measures. The approaches and analysis topics considered are innovative (there aren't many scientific documents on the topics that address bibliometric analysis and quantitative assessments with qualitative data) and bring novelty to the literature.

Warming enhanced the interaction effects of fungi and fungivores and soil potassium mineralization in tropical forest

Potassium (K) cycling in forest systems has received less attention than nitrogen (N) and phosphorus (P) cycles, despite its critical role in maintaining primary production and regulating water economy and use under climate warming. This study conducted a 10-year study in which we translocated dominant tropical forest tree seedlings and underlying soil (i.e., in situ soil) from high-altitude sites (600 m a.s.l.) to lower altitude sites at 300 m a.s.l. (+1.0 °C) and 30 m a.s.l. (+2.1 °C) to simulate climate warming in southern China. We investigated soil microbial and nematode communities, soil and foliar K concentrations at the aggregate level, and tree growth under different altitude sites. Microbial community was characterized by high-throughput sequencing, nematodes were identified to the genus level using microscope. The findings revealed that warming treatments significantly (p < 0.01) increased soil fungal gene abundance (+78 % at 300 m and +62 % at 30 m), fungal/bacterial abundance ratio (+121 % at 300 m and +101 % at 30 m), and soil fungivore abundance (+274 % at 300 m and +233 % at 30 m). These results indicate a shift in the soil decomposition pathway from bacterial to fungal-based channels. In addition, warming amplifies the interactions between fungi and fungivores. Aggregates had few effects on microbial biomass, but had significant effect on nematode abundance. Soil microcosm experiments consistently demonstrated that addition of the dominant fungivore Aphelenchoides significantly (p < 0.05) enhanced soil-exchangeable K by stimulating fungal gene abundance and activity. Furthermore, warming-induced changes in forest communities were observed. At the species-specific tree level, Syzygium rehderianum demonstrated the ability to take advantage of this scenario, exhibiting increased K uptake (−4% at 300 m and +32 % at 30 m). This may be attributed to the species being ectomycorrhizal-forming, where colonizing root surfaces can mobilize interlayer and structural K from the minerals. In contrast, Machilus breviflora exhibited lower foliar K concentrations (−42 % at 300 m and −41 % at 30 m) and reduced growth. However, warming had little effect on Schima superba, Myrsine seguinii, Itea chinensis, and Ardisia lindleyana growth. Warming-induced changes in the plant–soil system K cycle highlight the emergence of a new ecosystem structure with different soil web structures and distinct plant community species composition. Our results prompt further research to understand the microbiome-mediated complexities of understudied nutrient cycles, which are likely to be further altered in the future owing to climate change.

The challenge of cellulose nitrate-coated fabrics: molecular characterization of celluloid detachable collars and Fabrikoid

In the 19th century, significant linen and leather imitation products were invented using cellulose nitrate-coated fabrics, including celluloid detachable collars and Fabrikoid artificial leather, now preserved in the Hagley Museum and Library, USA. Using optical microscopy, µRaman, and µFourier Transformed Infrared spectroscopies, this study highlights the need for characterizing the heterogeneity of these materials at the microscale. While the detachable collars have well-preserved fabric coatings composed of cellulose nitrate, camphor, anatase (TiO2), and carbon-based particles, Fabrikoid’s pigmented cellulose nitrate-castor oil systems show problems. Our molecular data align with a 1922 report on Fabrikoid degradation, revealing free fatty acids and carboxylates formed due to oil oxidation. This is concerning as these materials were used until the 1960s, demonstrated by the analysis of objects from the National Museum of Costumes in Portugal. Future studies should address the compatibility of cellulose nitrate with fatty acids and the reactivity of additives in these systems.

Seed Quality and Growth of Soybean from Different Types of Soil and Cropping System

Seed Quality and Growth of Soybean from Different Types of Soil and Cropping System

Evaluation of the nutrient elements profiling of cucumber plants fertigated by three different nutrient solutions in soil and soilless culture systems

Evaluation of the nutrient elements profiling of cucumber plants fertigated by three different nutrient solutions in soil and soilless culture systems

Manipulating nature heatwaves in chambers: bridging controlled and field conditions for accurate warming study in plant and soil systems.

Abstract Understanding plant responses to climate variables such as heatwaves is crucial for plant biology research, necessitating growth environments that are highly controllable and reproducible. Traditional field methods lack control due to absent cooling systems and struggle to isolate field variables. Standard enclosures similarly fail to account for authentic environmental dynamics. To overcome these limitations, we developed AmbControl, a sunlit facility that bridges controlled environments with field conditions. AmbControl accurately captured the 2021 and 2022 heatwave fluctuations, allowing for the simulation of varying heat scenarios, including temperature scenarios ranging from +1.5°C to -4°C, and a stable 28.9°C. We observed differential temperature controls during night and day in the +1.5°C scenario, registering variations of ?1.0°C at night and ?1.8°C during the day. Temperature manipulations also inevitably altered relative humidity, highlighting its role as a confounding factor. The AmbControl system is essential for advanced climate impact studies, offering a platform for the next generation of agronomic experiments and climate resilience research.