The utilization of polybags is prevalent among agriculturalists as a nursery container in one of the processes in agricultural and plantation activities. The polybag currently used is derived from petroleum and tough to decompose, causing various environmental problems such as soil contamination which interferes with plant growth. On the other hand, Indonesia has a superior palm oil commodity that increases annually. This accumulation provokes an increasing quantity of palm oil waste. Oil palm empty fruit bunch (OPEFB) is the highest considerable solid waste in the CPO production (0.23 kg/kg fresh fruit bunch) containing cellulose (40.37%). It is potentially used as bio-polybags decomposable easily. This study aims to determine the preparation method and the composition’s consequence on the characterization of OPEFB cellulose-based-bio-polybags. The technique used in producing bio-polybags is solution casting. This study’s concentration variants were adjusted to the ANOVA (23 factorial design) statistical results by conversion regression equation concerning corn starch, cellulose acetate, and CaCO3. Mechanical assessments include tensile strength (1.461 – 8.442 MPa) and elongation (2.611% – 8.808%). The structural test might consider the quality of the biofilm. This innovation is desired to decrease conventional polybag usage and promote sustainable bio-polybag as eco-friendly nursery containers in the vegetable plantation.