Oil In Rats Research Articles

Ameliorative effect of α-tocopherol and curcumin against the deleterious effects of hydrogenated (thermally oxidized) rice bran oil in rats

Rice bran oil (RBO) is known for its health benefits due to its high content of natural antioxidants like tocotrienols, tocopherols, and oryzanols, which combat oxidative stress and inflammation. However, repeated heating of RBO above 110°C leads to degradation, forming harmful oxidative components and reducing its antioxidant content. This study aimed to investigate the effects of long-term consumption of thermally oxidized RBO on rats and the potential protective effects of curcumin and α-tocopherol supplementation. RBO was subjected to oxidative degradation through repeated frying cycles at temperatures ranging from 100°C to 180°C for 45 days. Physicochemical and phytochemical parameters of the oxidized RBO were analyzed. In vivo experiments evaluated serum biochemical, hematological, and histopathological parameters, along with oxidative and inflammatory responses in rats fed with oxidized RBO for 45 days. Results showed that thermal oxidation at 180°C significantly altered the chemical composition of RBO, increasing oxidative markers and reducing nutritional components. Consumption of oxidized RBO led to decreased body weight gain, elevated levels of various biochemical markers indicating liver and kidney dysfunction, hyperlipidemia, and increased oxidative and inflammatory damage in rats. However, supplementation with curcumin and α-tocopherol effectively mitigated these harmful effects. In conclusion, long-term consumption of thermally oxidized RBO negatively affects health parameters in rats, but fortification with curcumin and α-tocopherol helps alleviate these adverse effects

Mentha piperita and Thymus vulgaris ameliorate hematological changes induced by chronic consumption of oxidized palm oil in rats

Objective: The aim of this study was to investigate the effect of Mentha piperita L and/or Thymus vulgaris in ameliorating the hematological changes induced by chronic consumption of oxidized palm oil (OPO) in rats. Methodology: Fifty rats were divided into five groups as follows: rats received a basal diet (control), rats received a basal diet sustained with OPO, rats fed OPO and received Mentha piperita extract, rats fed OPO and received Thymus vulgaris extract, and finally rats fed OPO and received both extracts. After 6 weeks, blood samples were withdrawn and a complete blood count test was done. Results: Rats fed a diet containing OPO showed a significant decrease in red blood cells (RBCs), hematocrit (Hct), mean corpuscular volume (MCV), hemoglobin (Hb), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), lymphocytes (LYM), and platelets (PLTs) accompanied by a significant increase in white blood cells (WBCs), red cell distribution width (RDW), and mean platelet volume (MPV) compared to rats fed a normal diet. In contrast, rats fed on diet containing OPO and the aqueous extract of either Mentha piperita or Thymus vulgaris showed a significant increase in RBCs, Hct, MCV, Hb, MCH, MCHC, LYM, and PLTs accompanied by a significant decrease in RDW, WBCs, and MPV relative to rats fed on diet containing OPO only. Treatment with a combination of both plants showed better improvements. Conclusion: Both Mentha piperita and Thymus vulgaris ameliorate the hematological changes induced by OPO.

Antihyperlipidemic effect and GC-MS analysis of phytoconstituents from Laurus nobilis essential oil in rat

This study aimed to evaluate the effect of Laurus nobilis (L. nobilis) essential oil (EOs) (80 mg/kg) on Triton WR-1339-induced dyslipidemia in Wistar rats. The effect of L. nobilis essential oil (80 mg/kg) on lipid and lipoprotein profile was examined on Triton WR-1339-induced dyslipidemia in rats. Furthermore, the phytochemical evaluation was performed by GC-MS. In Addition, the acute toxicity of this EO was evaluated at a dose of 2 g/kg. The results revealed that the main constituents of L. nobilis EO were 1,8-cineole (39.5%), linalool (13.09%), and a-terpineol (11.55%). Furthermore, the EO did not cause any signs of toxicity or mortality, and the acute lethal dose 50 (LD50) was estimated to be higher than 2 g/kg. L. nobilis EO ameliorated lipid parameters and atherogenic indices. In conclusion, the study demonstrates that L. nobilis essential oils possess antidyslipidemic activity in acute model of hyperlipidaemia.

Study of the effectiveness of the drug Laennek in case of liver damage by palm oil in rats

Palm oil is a component of most food products, characterized by a high content of palmitic and oleic acids, an excess of which leads to the formation of tripalmitins, practically do not respond to enzymatic hydrolysis by lipoprotein lipase and predetermine the development of obesity, type 2 diabetes mellitus and atherosclerosis. This paper presents the results of a study of the drug Laennek, produced on the basis of standardized hydrolysates of the human placenta (HRP). The use of HRP under the influence of excessive consumption of palm oil in the diet led to a significant decrease in markers of liver dysfunction (ALT), which increase with an enriched diet of refined palm oil (bilirubin, total protein, ALT, AST). The hepatoprotective effect of Laennek was confirmed by the results of histological and biochemical studies indicating a decrease in the severity and prevalence of fatty liver dystrophy.

Antidiabetic Effect of Fig Seed Oil in Rats with Diabetes Induced by Streptozotocin.

The anti-diabetic effect of Ficus carica (Fig) seed oil was investigated. 4 groups with 6 rats in each group were used in the experiment as control, diabetes (45 mg/kg streptozotocin), fig seed oil (FSO) (6 mL/ kg/day/rat by gavage) and diabetes+FSO groups. Glucose, urea, creatinine, ALT, AST, GSH, AOPP and MDA analyses were done. Pancreatic tissues were examined histopathologically. When fig seed oil was given to the diabetic group, the blood glucose level decreased. In the diabetes+FSO group, serum urea, creatinine, AOPP, MDA levels and ALT and AST activities decreased statistically significantly compared to the diabetes group, while GSH levels increased significantly, histopathological, immunohistochemical, and immunofluorescent improvements were observed. It has been shown for the first time that FSO has positive effects on blood glucose level and pancreatic health. It can be said that the protective effect of fig seed oil on tissues may be due to its antioxidant activity.

Anti-inflammatory, antioxidant, antiangiogenic, and therapeutic efficacy of neroli oil in rats with endometriotic lesions.

Endometriosis is a serious health problem among women of reproductive age, with pelvic pain and infertility. Given the limited success of current treatments, this study explores Neroli oil (N.O.) effects on inflammation, oxidation, angiogenesis, and tissue remodeling implicated in endometriosis. Albino Wistar female rats were used to simulate an endometriosis model. Groups were established for comparison: a control, an endometriosis model, a N.O.-treated group, and a N.O.-treated group postendometriosis induction. The study focused on Tumor necrosis factor-alpha (TNF-α), Interleukin 6, Interleukin 8, vascular endothelial growth factor (VEGF), myeloperoxidase, Matrix metalloproteinase-1 (MMP-1), nitric oxide, superoxide dismutase, catalase, and anti-mullerian hormone values, as well as histopathological evaluations of endometriotic foci. AMH values showed a significant increase in the endometriosis group treated with N.O. compared with the endometriosis group (p < 0,01).A statistically significant decrease was found in MMP-1 level in the endometriosis group that underwent N.O. (p < 0.001). Increased CAT (p < 0.0001) and decrease in nitric oxide (p < 0.01) are found in N.O.-treated endometriosis group. TNF-α levels in the endometriosis group showed a statistically significant increase in the endometriosis group when compared with the control and sham group (p < 0.001, p < 0.01 respectively). In our study, a statistically significant increase was observed in VEGF levels (p < 0.001) in endometriosis group and significant decrease in the N.O. administered endometriosis model group. Groups treated with N.O. showed decreased inflammation and congestion scores. Histopathological assessments demonstrated reduced inflammation and tissue remodeling signs in endometriotic foci. This study highlights the potential of N.O. in the treatment of endometriosis, owing to its anti-inflammatory, antioxidant, and antiangiogenic properties that can disrupt chronic processes. Our findings lend support to utilization of herbal remedies for the management of endometriosis, thereby emphasizing the necessity for additional comprehensive investigations in the future.

Acute and subchronic oral toxicity study of Camelina sativa oil in Wistar rats

ObjectiveThe aim of these studies was to ascertain if Camelina sativa oil is harmful in both the acute and subchronic states. MethodsWistar rats of both sexes were used in an acute toxicity test, and the fatal dosage (LD50) of oral Camelina sativa oil was greater than 27.6 g/kg bw. Rats were gavaged with Camelina sativa oil at dosages of 0.00, 0.92, 1.84, and 3.68 g/kg bw per day for 90 days. In addition, satellite groups were established in the control and high-dose groups for a 28-day recovery period. The following factors were assessed: mortality, clinical anomalies, body weight, food intake, hematological, serum biochemistry, urine, gross necropsy, and histology. ResultsThere were no observable toxicity-related changes in any of the three dosage groups. There is no toxicological relevance to the change in the high-dose hematological indicator PLT at the conclusion of the recovery period because it was within the usual range for this strain in our laboratory. The test material did not result in any pathological alterations, according to a pathological examination. ConclusionSince the results of the current study, the no-observed-adverse-effect-level (NOAEL) for Camelina sativa oil in rats has been determined to be greater than 3.68 g/kg bw.

Chemical Composition of the Essential Oil of Catha edulis Forsk from Djibouti and Its Toxicological Investigations In Vivo and In Vitro

This work aimed to evaluate the chemical composition of essential oils from Catha edulis Forsk collected in the Day Forest of the Republic of Djibouti. Additionally, in vivo toxicity studies, biochemical profiling, and hematological tests were conducted to determine the biological activity of the investigated essential oils. Finally, in vitro tests were performed to investigate the antibacterial activity of the essential oils. The essential oils were obtained at yields of 0.75%. Chromatographic analysis identified 39 compounds, of which cathinone (81.4%) and cathine (10.55%) were determined as the two major components, representing 91.95% of the total composition. Catha edulis essential oil had a rat LD50 of 2500 mg/kg, indicating very low toxicity. Chronic exposure studies revealed that use of the essential oil in rats resulted in persistent stimulant action at dosages of 100 and 200 mg/kg, whereas the weight gain of control rats was faster than that of the essential oil-treated rats. Hematological parameters showed a significant increase in red blood cell counts, hemoglobin, hematocrit, and platelets in treated rats, which could indicate blood hyperviscosity that may increase the risk of blood clots and cardiovascular diseases. Furthermore, the investigated essential oil exhibited antimicrobial activity against several bacterial strains.

Study on the Action of Chia Seed Oil in Rats’ Model of Autism

AbstractThis article mainly focuses on elucidating the effect of chia seed oil (CSO) on autistic traits in offspring rats induced with valproic acid (VPA). Postnatal maturity monitoring are conducted on postnatal day 1–15, and behavioral tests are implemented on postnatal day 49, subsequently, body weight, tail and body length, oxidative stress, the activity of acetylcholinesterase, histopathological analysis, and immunohistochemical staining in the hippocampal serotonin are investigated to confirm the rat model of autism and evaluate the effects of different doses of CSO. Offspring exposed to VPA prenatally exhibits autism‐like behavior, delayed eye‐opening, olfactory dysfunction, slow growth, poor sociability, high anxiety, and oxidative stress. In addition, neuronal degeneration and cytoplasmic vacuolation are found, and positive areas of hippocampal serotonin are aggrandized. However, treatment with CSO, exceptionally high doses of CSO, significantly improves the poor growth and behavioral disorder, enhanced the protective effect on neurodevelopment, and reduced oxidative stress.Practical application: It can be deduced that CSO has antioxidant and neuroprotective effects, which can provide good protection for autism, especially since high doses of CSO have a significant impact.

Antioxidant Effects of Nigella sativa and Its Modulating Role on Hepatotoxicity Induced by Dietary Frying Oil in Rats

Repeated use of frying oil threatens — more than ever — human beings and this is due to the increased consumption of fried and fast foods. This study aimed to investigate the antioxidant effects of Nigella sativa against fryingoil-induced hepatic and oxidative alterations. In total, 40 male rats were divided into four groups and were treated during 30 consecutive days with frying oil and/or nigella. (EO-0) group: Rats were served as control and received a normal diet. (EO-N) group: Rats were fed with an experimental diet containing 2% nigella. (FO-0) group: Rats were nourished on diet containing 7% frying oil. (FO-N) group: Rats were received a combination diet containing 7% frying oil and 2% nigella. The physicochemical analyses indicate that frying oil contains very high concentrations of toxic substances compared to edible oil. The consumption of frying oil causes a significant increase in hepatic biomarkers and intensifies in oxidative stress status compared to the control group. Moreover, the hepatic histological sections of rats fed with frying oil showed a disrupted architecture. The combination of nigella with frying oils in rats’ diet revealed an intense reduction in the oxidative stress and ameliorated the levels of the majority of the previous parameters. Nigella supplementation confirmed their potential antioxidant activities. Therefore, administration of nigella seems to be the excellent spice recommended in high-temperature food frying in order to maintain human health.

Antioxidant Effects of Nigella sativa and Its Modulating Role on Hepatotoxicity Induced by Dietary Frying Oil in Rats

Repeated use of frying oil threatens more than everhuman beings and this is due to the increased consumption of fried and fast foods. This study aimed to investigate the antioxidant effects of Nigella sativa against frying oil-induced hepatic and oxidative alterations. Forty male rats were divided into four groups and treated for thirty consecutive days with frying oil and/or nigella. EO-0 group: rats were served as controls and received a normal diet. EO-N group: rats were fed with an experimental diet containing 2% nigella. FO-0 group: rats were nourished on a diet containing 7% frying oil. FO-N group: rats were given a combination diet containing 7% frying oil and 2% nigella. The physicochemical analyzes indicate that frying oil contains very high concentrations of toxic substances compared to edible oil. The consumption of frying oil causes a significant increase in hepatic biomarkers and intensifies the oxidative stress status compared to the control group. Moreover, the hepatic histological sections of rats fed with frying oil showed a disrupted architecture. The combination of nigella with frying oils in a rat-diet revealed an intense reduction in oxidative stress and ameliorated the levels of the majority of the previous parameters. Nigella-supplementation confirmed their potential antioxidant activities. Therefore, nigella-administration seems to be the excellent spice recommended in high temperature food frying in order to maintain human health.

MCC950 Ameliorates Acute Exogenous Lipoid Pneumonia Induced by Sewing Machine Oil in Rats via the NF-κB/NLRP3 Inflammasome Pathway.

Acute exogenous lipoid pneumonia (AELP) is a rare disorder caused by intake of lipid formulations and is often underdiagnosed. Meanwhile, the mechanism of AELP is still underlying. MCC950, was previously found to significantly suppress the release of inflammatory cytokines IL-18 and IL-1β. However, the effect of MCC950 on AELP induced by sewing machine oil has not been reported. The NLRP3, NF-[Formula: see text]B p65, caspase-1 and IL-1β expression in lung tissues were compared between a rat model of AELP and control rats using western blotting and real-time quantitative assay. Moreover, haematoxylin and eosin (H&E) staining was performed to elucidate the mechanisms by which MCC950 ameliorates sewing machine oil-induced AELP in vivo. MCC950 reduced the expression of NF-[Formula: see text]B p65 in the lung samples of the treatment group and further down-regulated the NLRP3 and caspase-1 levels while inhibited the production of IL-1β. Besides, decreases in inflammatory cell infiltration in the lung were shown using H&E staining. MCC950 ameliorates sewing machine oil-induced acute exogenous lipoid pneumonia in rats through inhibition of the NF-[Formula: see text]B/NLRP3 inflammasome pathway.

Effect of feeding strawberry, raspberry and rapeseed oil in rats’ diet on the fatty acid profile of muscle tissue

Abstract Currently, alternative plant oils with pro-health properties are sought. The aim of the present study was to investigate the effect of feeding strawberry, raspberry and rapeseed oils in rats’ diet on the fatty acid profile of muscle tissue. Adult rats were randomly divided into 4 groups (n=7) and fed with the addition of rapeseed, raspberry or strawberry seed oil, respectively, or control group. After a 6-week treatment period, the fatty acid profile in m. latisssimus dorsi, was analyzed using gas chromatography. The dietary strawberry or raspberry seed oil led to a significant increase in C-18:2 n-6 ad C-18:3 n-3 level in muscle fat when compared to control group. At the same time, in the group receiving raspberry oil, an unfavorable phenomenon of lowering the EPA content was observed, while a tendency towards a decrease in DHA level was observed in groups supplemented with both raspberry and strawberry oil. Both oils as a source of PUFA n-3 and n-6, increased ALA n-3 and LA n-6 fatty acids in latissimus dorsi muscle, but due to different activity of enzymes taking apart in conversion of polyunsaturated fatty acids to their long chain derivatives in rats, the research on pig model would be advisable.

Anti-Inflammatory Activity of Essential Oil from Zingiber ottensii Valeton in Animal Models.

Zingiber ottensii (ZO) Valeton, a local plant in Northern Thailand, has been widely used in traditional medicine. Many studies using in vitro models reveal its pharmacological activities, including the anti-inflammatory activity of ZO essential oil, extracted from ZO rhizomes. However, the scientific report to confirm its anti-inflammatory activity using animal models is still lacking. The present study aimed to evaluate the anti-inflammatory activity and explore the possible mechanisms of action of ZO essential oil in rats. The results revealed that ZO essential oil significantly reduced the ear edema formation induced by ethyl phenylpropiolate. Pre-treatment with ZO essential oil significantly reduced the carrageenan-induced hind paw edema and the severity of inflammation in paw tissue. In addition, pre-treatment with ZO essential oil exhibited decreased COX-2 and pro-inflammatory cytokine TNF-α expression in paw tissue, as well as PGE2 levels in serum. On this basis, our study suggests that ZO essential oil possesses anti-inflammatory activity in animal models. Its possible mechanisms of action may involve the inhibition of TNF-α expression as well as the inhibition of COX-2 and PGE2 production. These findings provide more crucial data of ZO essential oil that may lead to new natural anti-inflammatory product development in the future.

Protective effect of high-oleic acid peanut oil and extra-virgin olive oil in rats with diet-induced metabolic syndrome by regulating branched-chain amino acids metabolism

High-oleic acid peanut oil (HOPO) and extra-virgin olive oil (EVOO) have been reported previously to have an attenuating effect on metabolic syndrome (MS). This study aimed to evaluate the metabolic effect of HOPO and EVOO supplementation in attenuating MS and the role of gut microbiota in regulating the metabolic profile. Sprague-Dawley rats were continuously fed with a normal diet, high-fructose and high-fat (HFHF) diet, HFHF diet containing HOPO, or a HFHF diet containing EVOO for 12 weeks. The metabolomics profiles of feces and serum samples were compared using untargeted metabolomics based on UPLC-Q/TOF-MS. Partial Least Squares Discriminant Analysis (PLS-DA) was used to identify the potential fecal and serum biomarkers from different groups. Correlation between gut microbiota and biomarkers was assessed, and pathway analysis of serum biomarkers was conducted. Differences in metabolic patterns in feces and serum were observed among different groups. There were 8 and 12 potential biomarkers in feces and 15 and 6 potential biomarkers in serum of HOPO group and EVOO group, respectively, suggesting that HOPO and EVOO supplementation mainly altered amino acids, peptides, and their analogs in feces and serum. The branched-chain amino acids (BCAAs) biosynthesis pathway was identified as a major pathway regulated by HOPO or EVOO. This study suggests that HOPO and EVOO supplementation ameliorate diet-induced MS, mainly via modulation of the BCAAs biosynthesis pathway.

Evaluation of the Anti-ulcer Effect of Extra Virgin Avocado (Persea americana) Oil in Rats

Peptic ulcer disease is a gastrointestinal disorder of high mortality and morbidity. Therapy of this disease condition with conventional drugs presents therapeutic challenge due to incidents of unwanted side effects, and the high cost of these medications. The use of products from natural sources will provide useful alternatives due to their minimal side effects, being inexpensive, and readily available, especially in resource-challenged nations of the continent of Africa and other developing economies. Oils derived from plants are increasingly being used in management of several disease conditions. This study aims at evaluating the antiulcer activity of extra virgin avocado oil in rodents using ethanol-induced, and indomethacin-induced ulcer models. Test group was given extra virgin avocado oil (1ml per 250g weight) for 7 days before induction of ulcer. Positive controls received omeprazole 30 mg/kg, while negative control animals were given distilled water (10ml/kg) for 7 days respectively, before induction of ulcer. The stomachs were excised and their histopathological examinations carried out. Avocado oil significantly reduced the ulcer index in both models when compared to the negative control group, and histopathological findings corroborate that the oil ameliorates ulcerations in both models. The present study has demonstrated that extra virgin avocado oil possesses significant antiulcer activity.

Evaluation of antidiarrheal effect of combination of Salam Leaves (Syzygiumpolyanthum) and Jackfruit Leaves (Artocarpus heterophyllus Lam.) infusum in rats induced by castor oil

&#x0D; Backround: Diarrhoea is a condition characterised by watery, loose stools that occurs more than three times daily. In Indonesian traditional medicine, salam leaves and jackfruit leaves have been used as herbal treatments for many conditions, including as antidiarrheal medicines. &#x0D; Aims: The aim of this experimental study was to evaluate the antidiarrheal effect of a combination of Salam leaves and jackfruit leaves infusum induced by castor oil in rats. &#x0D; Method: The rats were divided into nine groups, where the negative control group was given CMC 1 %, the positive control group was given tannins, and five test groups were given the infusum with five comparisons of each dose administered orally. Castor oil was used as a stimulant of diarrhoea. &#x0D; Results: The results show that with all combinations of salam leaves: jackfruit leaves have antidiarrheal effects with decreased frequency of defecation, faeces consistency, and faeces weight at ratios of 1:1, 1:2, 1:3, 2:1, and 3:1 compared to the negative control (p &lt; 0.05). Phytochemical test of the infusum indicated positive tannins. The antidiarrheal effect of both infusums might be due to the presence of tannins, which have anti-secretory effect in the intestinal lumen. &#x0D; Conclusions: The treatment with combination of salam leaves and jackfruit leaves infusum in rats induced by Castor oil has an antidiarrheal effect. The best result is a mixture of salam infusum: jackfruit infusum with 3:1 ratio.&#x0D;

Improvement of memory deficits via acetylcholinesterase inhibitory activity of Nepeta nuda ssp. nuda essential oil in rats

Nepeta L. (Lamiaceae) species are belonging to aromatic and medicinal plants. Many biological activities of Nepeta species have been reported. In this study, memory-enhancing activities of Nepeta nuda ssp. nuda essential oil was aimed to be evaluated in laboratory rats treated with scopolamine. GC-MS/GC-FID system was used to identify oil composition. Memory performances of the rats were tested using Y-maze and radial-arm maze. Acetylcholinesterase enzyme activity and molecular docking analysis were also performed in this study. In total, 44 different compounds were identified in Nepeta nuda ssp. nuda essential oil. Trans-cis nepetalactone was showed to be the most abundant constituent in the oil. In the Y-maze test, the oil improved the performances of the animals on spontaneous alternation. Besides, in the radial-arm maze test, the oil improved working and reference memory significantly impaired in the rats subjected to scopolamine. The oil also showed acetylcholinesterase inhibitory activity in vitro. The molecular docking method, in the meantime, revealed that some of the compounds in the oil could interact with the acetylcholinesterase enzyme, theoretically. Nepeta nuda ssp. nuda essential oil improved spatial memory of scopolamine-induced rats. Neuroprotective effects of the oil are probably related to the anti-cholinesterase activity of the oil. Therefore, Nepeta nuda ssp. nuda essential oil could be used as a complementary therapy to improve memory in Alzheimer’s and related diseases.

Cardiac contractile dysfunction, during and following ischaemia, is attenuated by low-dose dietary fish oil in rats.

Supplementing animal diets with high-dose fish oil, rich in long chain omega-3 (ω-3) docosahexaenoic acid (DHA), enhances cardiac contractile efficiency and attenuates dysfunction, attributable to ischaemia. However, it remains unclear whether smaller doses, equivalent to what is achievable via regular fish consumption in the human diet, offer similar protection. Male Sprague-Dawley (12-15w) rats were fed isoenergetic diets (ad libitum) containing 10% fat by weight (22% energy) for 4-5w. Control diet (CON) contained 5.5% beef tallow; 2.5% ω-6 sunflower seed oil; 2% olive oil. Fish oil diets included high-DHA tuna oil exchanged for olive oil to provide 0.32% (FO1; human equivalent EPA + DHA 570mg/d) or 1.25% (FO2; equivalent EPA + DHA 2.3g/d) wt/wt dose of fish oil. Anaesthetised rats (pentobarbital: 60mg/kg i.p.) were subjected to 45min coronary artery occlusion then reperfusion in vivo as a whole animal model of regional myocardial ischaemia, with left ventricular haemodynamic function measured by conductance catheter. Ischaemia-induced reductions in rate pressure product recovered faster in the FO2 group and post-ischaemic left ventricular pressure-volume loop integrity (shifted downwards and right in CON) was partially protected in both fish oil groups. Ischaemia-induced contractile dysfunction in rats is limited from fish oil doses equivalent to regular consumption of fish in the human diet. These observations highlight plausible and clinically relevant physiological changes that rationalise nutritional conditioning of the heart with DHA for on-going cardioprotection.

Renoprotective effect of peanut oil on experimentally induced chronic renal failure in rats

The renoprotective effect of Peanut oil in rats suffered from chronic renal failure with adenine was evaluated. Adult male albino rats were classified as follow: normal group, Peanut oil group (0.75ml/kg), adenine fed group (0.75% w/w), the 4th group received both adenine in concomitant with peanut oil by the same dose and route as in the above groups, for 4 weeks. The antioxidant activity, total phenolic and flavonoid content of Peanut oil was estimated. Adenine administration reduced body weight, exceed the relative kidney weight as well as excessive serum creatinine, uric acid and BUN. In addition, exceed urine volume, appearance of urinary protein associated with significant increased urine NAG activity, while urinary Creatinine and creatinine clearance were decreased. It raised serum concentrations of inflammatory markers (C-RP, TNF-α, IL-6 and IL-10) relative to control group. Histopathological changes were noticed including tubulointerstitial atrophy and fibrosis. These kidney functions abnormalities were associated with increased renal oxidative stress markers (MDA and XO), decreased renal antioxidants markers (SOD, CAT, GSH and TAC). Administration of Peanut oil with adenine suppress the hazardous effect of adenine, where they keep most of the estimated parameters within normal. In conclusion, these results indicate that the use of Peanut oil has efficient protective effect against renal injury in adenine fed rats.