Oil Extract Research Articles

Test of the Effectiveness of Giving Salmon DNA Serum with Virgin Coconut Oil Extract on the Growth of Skin Tissue from Cut Scars on the Skin of Female Wistar Rats (Rattus Norvegicus)

Various treatments can cure pockmarks. First, you have to know the cause of the pockmarks and the strength of the pockmarked skin, making it easier to choose the treatment, one of which is dermapen, which uses plasma from platelets. Test and compare the effectiveness of the derma pen action using platelet plasma with the derma pen action on the pockmarked skin surface of female Wistar rats (Rattus novergicus). This research is a laboratory experiment to compare the effectiveness of the dermapen action using plasma from platelets with the dermapen action on the pockmarked skin surface of female Wistar rats (Rattus novergicus) using a post-test with control group design or control samples based on treatment groups to analyze the dermapen action using plasma from platelets with dermapenic action on the surface of rat skin pockmarks. The results showed that the dermapen treatment group with plasma from platelets at a dose of 10% was more effective in healing cut wounds in white mice than the dermapen treatment group using plasma from platelets at doses of 2.5% and 5%. This is because, at a concentration of 10%, blood plasma metabolite compounds from platelets that are applied to wounds already affect the wound. The effect only inhibits microorganisms at small doses, making it less effective in healing wounds. It is recommended that the minimum dose be 10% so that the metabolite compounds in the plasma from platelets have a healing effect on wounds.

Green Synthesis of Zinc Oxide Nanoparticles Using Clove Oil Extract (Syzygium aromaticum) and Evaluate their Antibacterial Activity against MRSA

There is a crucial need for the development of effective antimicrobial agents due to the rising incidence of hospital-acquired bacterial infections caused by multidrug-resistant microbes, leading to significant mortality and morbidity worldwide. Therefore, the current study aimed to synthesize zinc oxide nanoparticles (SaZnONPs) using clove extract through an environmentally friendly technique and to investigate their antibacterial activity against MRSA (Staphylococcus aureus). Clove extract acts as both a capping and reducing agent in the formation of ZnO-NPs. The formation of SaZnONPs was confirmed using various analytical techniques. Initially, clove extract was incubated with a zinc nitrate solution, and the subsequent change in color of the solution indicated the reduction of zinc nitrate ions to ZnO nanoparticles. UV-visible spectroscopy revealed a surface plasmon resonance band of SaZnONPs at 329.26 nm. Additionally, SEM imaging indicated that the nanoparticles had an average size of 41 nm and exhibited a spherical shape. The antibacterial activity of SaZnONPs was evaluated against MRSA using agar well diffusion and minimum inhibitory concentration assays. The phytochemicals present in clove extract facilitated the synthesis of stable ZnO-NPs, demonstrating promising antibacterial properties.

In vitro Antifungal Efficacy of Some Plant Extracts against Fungal Pathogens Causing Diseases in Solanaceous Crops

Background: This research aimed to assess the antifungal potential of oil and organic leaf extracts obtained from Ocimum gratissimum, Kopsia fruticosa and Hibiscus rosa-sinensis against fungal pathogens affecting solanaceous crops. Methods: Oil of O. gratissimum was obtained by hydro-distilled method using Clevenger apparatus. Leaf extracts of K. fruticosa and H. rosa-sinensis were extracted using three organic solvents namely hexane, ethanol and methanol. The antifungal activity of the oil and organic leaf extracts against the test fungal pathogens was determined by the agar well diffusion method. The efficacy of the oil and organic leaf extracts against the pathogens was evaluated by their minimum inhibitory concentration (MIC), ability to inhibit spore germination and growth kinetic assay. Result: The oil of O. gratissimum (1000 ppm) significantly inhibited the growth of Cercospora capsici (81.4%), Alternaria alternata (73.7%) and Fusarium oxysporum (68.7%). Hexane extract of K. fruticosa and methanol extract of H. rosa-sinensis (1500 µg/well) displayed strong antifungal activity against Colletotrichum acutatum with inhibition percentages of 75.9% and 82.4% respectively. Similarly, ethanol extract of K. fruticosa and methanol extract of H. rosa-sinensis showed effective inhibition against C. capsici and A. alternata. The oil of O. gratissimum showed MIC values of 50, 100 and 200 µg/mL against C. capsici, A. alternata and F. oxysporum respectively. Ethanol extract of K. fruticosa showed MIC values ranging from 100 to 350 µg/mL, while H. rosa-sinensis methanol extract displayed MIC values ranging from 50 to 200 µg/mL against the fungal pathogens. The oil extract also demonstrated strong inhibition of spore germination of the test fungi. Our study suggests the potential of plant-derived essential oils and extracts as effective antifungal agents for crop protection.

Common Bacillus mitigate tomato verticillium wilt and bacterial specks when combined with an essential oil extract

This study was conducted to evaluate the effect of growth promoting rhizobacteria (PGPR) alone and in combination with an essential oil (EO) extracted from Tetraclinis articulata (Vahl) Masters in protecting tomato plants against verticillium wilt and bacterial speck diseases. Three strains, identified as Bacillus toyonensis EI, Bacillus thuringiensis ES and Bacillus thuringiensis RA, possess in vitro PGPR activity such as the production of indole acetic acid and ammonia, and the solubilization of phosphates. In planta, they significantly increased the stem length, number of leaflets, leaf area, and root biomass. EO and the three strains, alone or in combination, were able to enhance photosynthetic parameters such as net photosynthesis and transpiration rate. They also significantly reduced leaf alteration (LA) index, stunting index (SI) and browning index (BI) caused by Verticillium dahliae kleb. In tomato. Reduction of LAI ranged from 25% to 37%, and it enhanced to more than 60% when plants were treated with EO combined with B. thuringiensis SE or with B. toyonensis EI, indicating a synergistic effect. Moreover, EO as well as the three Bacillus strains were also able to enhance tomato resistance against bacterial speck disease caused by Pseudomonas syringae pv. tomato. The average number of lesions was significantly reduced by the application of EO and the three strains, reaching 94% with combination of EO with B. thuringiensis RA. Bacterial population analyses showed that plants with reduced symptoms exhibited significantly lower bacterial concentrations. In tomato plants inoculated with V. dahliae results showed that EO as well as three strains were able to enhance H2O2 accumulation, at the late stage of infection, which might lead to reduce disease. However, within the pathosystem tomato/P. syringe pv. tomato, H2O2 accumulation was highly enhanced by the pathogen and inhibited after combined treatment.

Wound Healing Effect of Lippia sidoides and Myracrodruon urundeuva Nanogel.

Wound healing is a natural regenerative response to tissue injury and the conventional treatments consists of the use wound dressings with local administration of medicines, but, in some cases, are only partially effective and limited by toxicity or ineffective anti-microbial protection. Medicinal plants such as Lippia sidoides and Myracrodruon urundeuva have shown interesting pharmacological activities, allied to this, the association of these medicinal plants and nanotechnology, could mean an advantage in relation to classical approach. This study investigated the effect of a nanogel loaded with Lippia sidoides essential oil and Myracrodruon urundeuva extract (NAA) in an excisional wound healing model in rats. Animals were anesthetized and skin wounds were made using a metal punch. The groups were treated with vehicle, NAA or collagenase gel, for 7, 14 or 21 days and then sacrificed for tissue analysis. NAA did not show acute dermal irritation, further significantly reduced (p<0.05) the final wound area, accelerated the wound contraction and organization of collagen in the group treated for 14 days. The data presented here demonstrate the therapeutic potential for the use of nanotechnology associated with medicinal plants and provides evidence that corroborate with the use of L. sidoides and M. urundeuva as healing medicinal plants.

Effect of paprika extracts on growth performance, haemolymph chemistry, intestinal microbiota and antioxidant enzyme activities of white-leg shrimp (Litopenaeus vannamei).

This study aims to evaluate the effects of paprika extract on the survival rate, growth performance and stimulation of the innate immune system of Litopenaeus vannamei. In this experiment, 240 healthy shrimp (3.22 ± 0.12 g) were randomly divided into four groups. The shrimp were fed diets with different concentrations of paprika oil extracts (0%, 0.5%, 1% and 2%) for 8 weeks. The results showed that growth performance, urea, uric acid, creatinine, cholesterol levels, aspartate aminotransferase and alkaline phosphatase activities were not significantly affected by adding paprika extract to the shrimp diet (p > 0.05). Diets containing 1% and 0.5% paprika extract showed the highest levels of total protein and triglyceride, respectively (p < 0.05). There was a significant decrease in haemolymph glucose concentration in shrimp-fed diets containing 1% and 2% paprika extract (p < 0.05). Moreover, a diet containing 0.5% paprika extract resulted in the highest levels of total heamocyte count, hyaline cells and large-granular cells in shrimp (p < 0.05). Higher catalase and superoxide dismutase activities were also exhibited in the paprika groups (p < 0.05). Vibrio sp. bacteria were not significantly reduced by paprika extract in the intestines of L. vannamei (p > 0.05). A significant decrease in heterotrophic bacteria was observed with increasing extract concentrations (p < 0.05). The shrimp culture industry can utilize paprika extract as a cost-effective, efficient and environmentally friendly immune stimulant at a concentration of 0.5%.

Two-dimensional correlation infrared spectroscopy elucidated the volatilization process of the microemulsion composed of peppermint essential oil and composite herbal extract

Microemulsion is usually a transparent and isotropic liquid mixture composed of oil phase, water phase, surfactant and cosurfactant. The surfactant-framed nanoscale droplets in the microemulsion can penetrate into the skin surface to reduce its barrier function. This makes microemulsion an ideal preparation for the transdermal drug delivery. The permeability of microemulsion may be further enhanced when botanical essential oils that can dissolve the stratum corneum are used as the oil phase. However, the volatility of essential oils is possible to shorten the retention time of the microemulsion on the skin surface. Therefore, analytical methods are required to understand the volatilization process of the microemulsion composed of essential oils to develop the reasonable topical drug carrier system. In this research, Fourier transform infrared (FTIR) spectroscopy with an attenuated total reflection (ATR) accessory cooperated with two-dimensional correlation spectroscopy (2DCOS) to elucidate the volatilization processes of some microemulsions composed of peppermint essential oil. Principal component analysis (PCA) and moving-window two-dimensional correlation spectroscopy (MW2DCOS) revealed the multiple stages of the volatilization processes of the microemulsions. Synchronous 2D correlation infrared spectra indicated the compositional changes during each stage. It was found that the successive volatilizations of ethanol, water and menthone were the major events during the volatilization process of the microemulsion composed of peppermint essential oil. Ethanol can accelerate the volatilization of water, while the composite herbal extract seemed to not influence the volatilization of the other ingredients. After a 20-min-long volatilization process, the remaining microemulsion still contained considerable peppermint essential oil to affect the skin. The above results showed the feasibility of developing the microemulsion composed of peppermint essential oil for the transdermal drug delivery of composite herbal extract. This research also proved that the combination of ATR-FTIR spectroscopy and 2DCOS was valuable to study the volatilization process of the microemulsion.

Total Cholesterol and Triglyceride Levels after Giving Olive Oil and Fig Fruid Extract Against Rattus Novergicus With High-Fat Diet

The reserach entiled “Changes in total chol; esterol levels and trigelyceride levels after administration of olive oil and fig leaf extract “was conducted from July to September 2024. This study aims to determine changes in total chplesterol levels and triglyceride levels when given olive oil and fig friut extract to white mice on a high-fat diet. Based on the results of stufy, constant result was obtained for total cholesterol in all treatment groups, namely 86.5 mg/dL and significant results were obtained in reducing triglycerides in the olive oil treatment group and the combination of olive oil and fig fruit extract, namely in the group given olive oil of 170.5 mg. /dL (81.19%), in the group given a combination of fig fruit extract it was 43.75 mg/dL (38.55%), and the increase in the group given fig fruit extract was 38 mg/dL (29.92%). The combination of olive oil and fig fruit extract has a constant effect on total cholesterol levels and reduces triglyceride levels. Fig fruit extract has a constant effect on total cholesterol levels and increases triglyceride levels. Olive oil has a constant effect on total cholesterol levels and more significant results in reducing triglyceride levels in mice with high-fat feed in all treatment groups.

Total phenol content of turmeric extract (curcuma longa L.) in vegetable oil using the ultrasonic method

Turmeric extract (Curcuma longa L.) in vegetable oil is an herbal oil rich in antioxidants called phenolics. Vegetable oil has the potential to be utilized as a solvent in herb extraction because it can extract bioactive components in plants because it is stable, safe, and ecologically friendly. The purpose of this study is to determine the secondary metabolites and total phenolic content of turmeric extract in Extra Virgin Olive Oil (EVOO) and Virgin Coconut Oil (VCO) with the addition of a surfactant (tween 80). The extraction method utilized was ultrasonic, with variations in surfactant dosage (0 - 30 g) and extraction time (10 - 25 minutes). FTIR spectrophotometer used to identify functional groups in herbal oils. The qualitative test showed that phenolic components, flavonoids, terpenoids, and alkaloids were detected in turmeric extracts EVOO and VCO both without and with tween 80. Based on statistical study, the extraction time and the amount of surfactant used significantly affect the overall phenol content of herbal oils. The maximum total phenolic content of turmeric extract EVOO and VCO was achieved after a 25-minute extraction with 30 g surfactant, namely 44.11 0.43 mg GAE/g and 35.22 0.43 mg GAE/g. Identification of the functional groups of herbal oils with the addition of tween 80 obtained absorption patterns namely O-H, C=O, C=C aromatic, C-O-C, and C-H.

Formulation, Evaluation and Optimization of Antimicrobial Potential of Herbal Cream Containing Allium sativum, Moringa oleifera Extracts and Thymus vulgaris Oil.

Herbal preparations can be formed by combining several plant classes. One possible explanation for the effectiveness of combined medications is that the various mixtures with different mechanisms may add up to produce a more comprehensive therapeutic effect. This study aims to investigate the synergistic antibiotic potential of a cream containing three natural herbal extracts: Allium sativum, Moringa oleifera, and Thymus vulgaris. The efficacy of combining these plant extracts was compared to that of a standard antibiotic formulation (Polyfax). The herbal cream was formulated by using aqueous extracts of garlic (Allium sativum), moringa (Moringa oleifera) and essential oil of thyme (Thymus vulgaris). The study aimed to explore the therapeutic potential of these extracts against bacteria. P. aeruginosa, B. subtilis, E. coli, S. aureus, and S. pneumonia are commonly found in fresh wounds. The results showed that garlic extract (5%) had the highest zone of inhibition, 14.26 ± 0.05 mm, and a combination of garlic (5%) and thyme (2%) exhibited a significant synergistic effect, with a 23.5 ± 0.05 mm zone of inhibition. High-performance liquid chromatography analysis revealed the presence of allicin, quercetin and thymol as potential therapeutic phytoconstituents. The formulated herbal cream had a soft texture, was easily spreadable, and had better stability and absorption than the standard polyfax. The topical application of the cream did not cause any skin reaction or allergy in mice. The in vivo wound healing effect of the herbal cream was investigated on an abrasion model of albino mice, and the results showed that the treatment group (46 ± 16.31%) had significant wound healing potential compared to the standard (64 ± 17.49%) and control groups (18 ± 3.74%). The formulated herbal cream was a better alternative to standard therapy, exhibiting promising healing and antimicrobial effects with significant compatibility and safety profile.

GC/MS and LC-MS Analysis and in-vitro Antioxidant Activity of Essential Oil and Crude Methanol Extract from the Leaves of Acacia Gerrardii Benth. Growing in Saudi Arabia.

In this study, I determined the essential oil (EO) chemical composition and crude methanol extract (ME) phytochemical profile of the leaves of Acacia gerrardii (ACGL), a plant growing in Saudi Arabia. Additionally, I assessed their in vitro antioxidant activity. The gas chromatography-mass spectrometry analysis of the EO revealed a high content of oxygenated monoterpenes (79.86 %), primarily dominated by pulegone (35.11 %), carvacrol (27.36 %), and neo-dihydrocarveol (4.67 %). The ME was analyzed by liquid chromatography-mass spectrometry to determine its qualitative chemical profile. Four organic acids, eleven phenolic compounds, sixteen flavonoids, nine terpenoids (eight triterpenoids and one diterpenoid), and one coumarin were found in the ME of ACGL. This extract was found to be dominated by 5,6,4-trihydroxy-7,3-dimethoxyflavone (39.30 %), acteoside (30.27 %), nevadensin (7.55 %), isoacteoside (3.08 %) and apiin (3.23 %), and hesperidin (2.73 %). The phenolic (TPC=127.70±1.47 mg gallic acid equivalents/g of extract) and flavonoid (TFC=85.48±0.12 mg quercetin equivalents/g of extract) contents of the ME were also assessed. The in vitro antioxidant activities of both the EO and ME were evaluated using DPPH, ABTS, and ferrous ion chelating effect assays. Compared to the positive control (vit. E and Vit. C), and both extracts exhibited excellent activity.

Production of essential/fragrance oil from pineapple peel extract for direct perfume formulation to enhance efficient waste management

Sustainable and effective waste management is an enormous task all over the globe especially in developing countries like Nigeria. And poor waste management basically is termed to be the second most troublesome issue affecting developing countries after portable water. Therefore, pineapple peel which is a solid waste was employed for the production of essential/fragrance oil using soxhlet extraction method and the oil extract were further used for perfume formulation with the application of n-Hexane as the solvent media. At the temperature of “65 °C” and “150 minutes”, the maximum oil yields obtained during extractions were “1.96%” and “1.97%” respectively. The quality and strength of the perfume obtained after formulation falls in the ranges of “15 to 20%” fragrance concentration of Eau de Parfume that can last 4 to 5 hours. Some major physiochemical parameters of the pineapple peel such as pH, API and acid values recorded 3.79, 27.476 and 2.946 mg KOH/g respectively. Other parameters were density and specific gravity also have 0.8765 and 0.8875 @ 20 ᵒC indicating lightness above water, but the viscosity was 0.757 kg/L which was higher than the viscosity of water. Also, refractive and ester index were 9.35 and 6.404 respectively.

Anti-lipidemic and protein restoration potential of Monodora myristica (Gaertn.) in rats fed with cassava containing crude oil

Consumption of cassava meal affected by crude oil has significant effects on lipid and protein metabolism. The hepatoprotective action of spices is mostly attributed to the suppression of lipid oxidation and protein breakdown. This study examined the protein restoration and anti-lipidemic potential of Monodora myristica (Gaertn.) in rats fed with cassava contaminated with crude oil.&#x0D; The research involved 36 albino rats separated into six groups (n = 6). Group 1 (control) consumed cassava without crude oil. Group 2 received cassava with crude oil. Groups 3, 4, and 5 fed on cassava with crude oil and various extracts of M. myristica, i.e., aqueous, ethanol, and diethyl ether, respectively. Group 6 received non-ionic synthetic surfactant Tween 80. The experiment relied on standard methods.&#x0D; Blood serum and liver obtained from the rats of Group 2 showed a significant (p &lt; 0.05) increase in total cholesterol, low density lipoprotein cholesterol, triacylglycerol, and malondialdehyde, as well as a decrease in total protein, albumin, and high-density lipoprotein cholesterol. The groups that received M. myristica extracts showed a significant increase (p &lt; 0.05) in total protein, albumin, and high-density lipoprotein cholesterol. They also had lower total cholesterol, low density lipoprotein cholesterol, triacylglycerol, and malondialdehyde as compared to Group 2, which dieted on cassava contaminated with crude oil without additives.&#x0D; In this research, crude oil-contaminated cassava affected proteins and lipids in rats. Diethyl ether extract of M. myristica demonstrated the best anti-lipidemic and protein restoration.

Extraction Characterization of Oils Extracted from different Parts of Red and Yellow Varieties of Anacardium occidentale (Lin)

The objective of this study was to extract and characterized the oils from different parts of red and yellow varieties of Anacardium occidentale after extractions using Soxhlet extractor and steam distillation techniques and characterization of the bioactive components using GC/MS analysis. Physicochemical parameters of the extracted oils were analyzed, and the bioactive compositions were also examined. The results showed that the oils extracted were within 0 and 0.5% moisture content and pH values ranged from 3.7 to 4.7. The average relative densities of the extracted oils by Soxhlet extraction and steam distillation at temperature 25oC were 0.887 and 0.8745 g/cm3 respectively, with average saponification value of 132.45 mgKOH/g for Soxhlet extraction and 127.98 mgKOH/g for steam distillation. The iodine values of all the oils were between 35 and 19.00 mg iodine/100g; while the refractive index was between 1.7 and 1.9. The acid values were within the range of 10.00 and 13.00 mgKOH/g, and peroxide value of 1.67 and 1.20 mmol/l. The oils had low viscosity which were within 28 and 32 mpa.s. The compounds identified from the GC-MS results showed that cashew nut shell oil contained cardol, anacardic acid, cardanol, 2-methyl cardol, triacconten and β-sitosterol. The main bioactive components of the extracted oil identified from the stem bark, root bark, and leaf were alkaloids, tannins, flavonoids, coumarins, terpenoids, and saponins. This study, therefore, showed that Soxhlet extraction could be a better extraction method for extraction of oil from cashew nut shell which had higher relative density; while cashew stem bark, root bark and the leaf which had lower density were selective based on the target products.

New insights on phytocannabinoids role in antioxidant activity of hemp inflorescences extract in high-oleic sunflower oil

Hemp (Cannabis sativa L.) inflorescences contain bioactive compounds potentially able to counteract lipid oxidation in vegetable oils. In this study, two hemp inflorescences extracts with (E) and without (wE) phytocannabinoids were tested at 0.02, 1.0, and 2.0 g/100 g of high-oleic sunflower oil (HOSO) exposed to thermo-oxidative conditions (90 °C; pO2, 600 KPa). Immediately at the end of induction time, the oxidative stability index (OSI), peroxide value (PV), main lipid classes, phytosterols, phytosterols oxidation products (POPs) content, and volatile organic compounds were determined. E extract added at 2.0 g/100 g proved to be more effective than wE extract, extending the OSI by almost 30 % with respect to control. The lowest PV (p < 0.01) was observed in the oil containing E (2.0 g/100 g), which also proved to significantly reduce the POPs content (p < 0.05). Both extracts had no effect on lipolysis during the entire experiment. The principal component analysis highlighted different antioxidant activity related to both type of extract and their concentration. These results suggest that hemp inflorescences could be a source of natural preservatives for vegetable oils even under severe oxidative conditions and confirm that phytocannabinoids play a key role in the modulation of antioxidant activity.

Insecticidal activities of the ethanolic extract of citrus fruit seeds for the control of Culex mosquitoes

Mosquitoes are among the significant vectors of public health concern. Culex mosquitoes have been implicated in spreading disease pathogens that inflicted both animal and man. The Hazards caused by using synthetic insecticide in controlling this vector cannot be overlooked. Thus, there is need to find alternative control method that is harmless and environment friendly. This research aimed to study the insecticidal activities of extract from four Citrus species seeds in the control of larval and adult stages of Culex mosquitoes. Different concentrations (1.0, 1.5, 2.0, 2.5, and 3.0%) of the extract was prepared using extraction solvent (ethanol) The highest percentage mortality of Culex larval was observed from application of 3.0% concentration of C. paradisi seeds extract within the period of exposure, 35.00%, 58.33%, 80.00%, and 100.00% larval mortality was recorded respectively. There is a significant difference (p < 0.05) comparing control and all the concentrations of Citrus seeds extract. Larvicidal potency of 3.0% seed extract concentration of C.sinensis, C.aurantium, C.aurantifolia, and C.paradisi are not statistically different (p > 0.05). The adulticidal potency of the seed oil varies with Citrus species. Citrussinesis seed oil gave higher percentage of adult Culex mortality in all the period of exposure. Statistically, the adulticidal potency of 2.5% concentration of all the Citrus seed oil are not different significantly (p > 0.05), expect the potency of C.aurantium seed extract. The lethal concentration requires for 50% (LC50) and 90% (LC90) of Citrus seeds oil extract varies with respect to period of exposure. Extract from Citrus seeds should be adopted for the control and management of insect vectors and it should be incorporated to integrated vector management (IVM) program.

Hypoglycemic and hypolipidemic activity of a catfish oil and Andrographis paniculata herb extract combination in diabetic rat model

The single use of natural ingredients for the treatment of diabetes mellitus is considered less optimal compared to the combination of several ingredients which is expected to produce a synergistic effect. Therefore, this study aimed to investigate the combined effect of catfish oil (CFO) and Andrographis paniculata ethanolic extract (APE) in reducing blood glucose levels and hyperlipidemia in a diabetic rat model. A total of 30 male Wistar rats were used while diabetes was induced using 50 mg/kg streptozotocin. The rats were randomly divided into six groups consisting of the control, diabetes, and drug control group treated with metformin 120 mg/kg; as well as treatments I, II, and III given CFO 1000 mg/kg, CFO 1000 mg/kg + APE 200 mg/kg, and CFO 1000 mg/kg + APE 400 mg/ kg, respectively. The test was carried out for 28 days, then on the 29th day, all the test animals were examined for observation of blood glucose levels and lipid profiles. In the fourth week of testing, all groups showed a significantly different blood glucose profile compared to the diabetes control group with p&lt;0.05. The combined administration of 1000 mg/kg CFO and 400 mg/kg APE showed a better reduction in blood glucose compared to the single dose of CFO (p&lt;0.05). Furthermore, the lipid profiles showed a significant reduction compared with the diabetes control (p&lt;0.05). Except for LDL values, the combination of both doses of CFO+APE showed a better reduction in lipid profile compared to the single CFO. A single dose of CFO and a combination dose of APE provided a beneficial effect by inhibiting the increase in blood glucose levels and lipid profiles in a streptozotocin-induced diabetic rat model. Based on the results, the combined dose of 1000 mg/kg CFO+400 mg APE produced a better effect on most of the blood biochemical parameters than the single administration of CFO

Phytochemical, Technological, and Pharmacological Study on the Galenic Dry Extracts Prepared from German Chamomile (Matricaria chamomilla L.) Flowers

Galenic preparations of German chamomile are used to treat mild skin diseases, inflammation, and spasms, and they have also been reported to have anxiolytic and sedative effects. The medicinal use of chamomile is well known in ethnomedicine. After obtaining its galenic preparations, there is lots of waste left, so it is expedient to develop waste-free technologies. The aims of this study were to gain knowledge of the ethnomedical status of chamomile in the past and present, develop methods for preparing essential oils and dry extracts from German chamomile flowers using complex processing, reveal the phytochemical composition of such extracts, and verify the analgesic and soporific activity of the extracts. Two methods for the complex processing of German chamomile flowers were developed, which allowed us to obtain the essential oil and dry extracts of the tincture and aqueous extracts as byproducts. A total of 22 phenolic compounds (7 hydroxycinnamic acids, 13 flavonoids, and 2 phenolic acids) were found in the dry extracts by using UPLC-MS/MS. In total, nine main terpenoids were identified in the chamomile oil, which is of the bisabolol chemotype. During the production of chamomile tincture, a raw material–extractant ratio of 1:14–1:16 and triple extraction are recommended for its highest yield. In in vivo studies with mice and rats, the extracts showed analgesic activity and improvements in sleep. The highest sedative and analgesic effects in rodents were found with the dry extract prepared by using a 70% aqueous ethanol solution for extraction at a dose of 50 mg/kg. The developed methods for the complex processing of German chamomile flowers are advisable for implementation into the pharmaceutical industry to reduce the volume of waste during the production of its essential oil and tincture, and to obtain new products.

Exploring the Influence of the Selected Conjugated Fatty Acids Isomers and Cancerous Process on the Fatty Acids Profile of Spleen

The spleen, traditionally associated with blood filtration and immune surveillance, has recently been recognized for its role in systemic lipid metabolism and potential influence on cancer development and progression. This study investigates effects of dietary supplements, specifically conjugated linolenic acids from pomegranate seed oil and bitter melon extract, on the fatty acid (FA) composition of the spleen in the context of cancerous processes. Advanced methods, including gas chromatography–mass spectrometry and silver ion-impregnated high-performance liquid chromatography, were employed to analyze the spleen’s FA profile. Our research uncovered that dietary supplementation leads to alterations in the spleen’s FA profile, especially under the carcinogenic influence of 7,12-dimethylbenz[a]anthracene. These changes did not align with a simple protective or anti-carcinogenic pattern, as previously suggested in in vitro studies. We observed shifts in conjugated FA isomer concentrations and variations in desaturase activities, suggesting disrupted lipid metabolism in cancerous conditions. The findings underscore the spleen’s vital role in lipid metabolism within the body’s systemic health framework, highlighting the complexity of dietary supplements’ impact on FA profiles in the spleen and their potential implications in cancer progression and treatment. This study adds valuable insight into the complex interplay between diet, disease, and metabolic regulation, particularly in cancerous environments.

Intramolecular Ring-Chain Equilibrium Elimination Strategy for Pinpointing C═C Positional and Geometric Isomers of N-Alkylpyridinium Unsaturated Fatty Acid Derivatives via Ion Mobility-Mass Spectrometry.

Free unsaturated fatty acids (UFA) are key intermediates of lipid metabolism and participate in many metabolic pathways with specific biological functions. Although various fragmentation-based methods for pinpointing C═C locations in UFA were developed, the current mass spectrometry methods are difficult to simultaneously differentiate geometric isomers and positional isomers in trace samples due to low ionization efficiency, low conversion, and low resolution. Herein, an intramolecular ring-chain equilibrium elimination strategy via 4-plex stable isotope labeling dual derivatization-assisted ion mobility-mass spectrometry was developed, thereby one-pot specifically labeling C═C and carboxyl groups among the trace and unstable UFA with high sensitivity, high efficiency, and good substrate generality. It achieved fast separation of both C═C positional and geometric isomers with high resolution, which benefited from eliminating the intramolecular ring-chain equilibrium by suppressing the formation of salt bridges between free carboxyl groups and pyridine cations. 4-plex stable isotope labeling reagents showed similar reactivity, enabling high-throughput quantitative analysis of omics. This method was successfully applied for accurate and rapid identification of the UFA composition in olive oil extract. These results suggest that the developed method provides new insight for rapid characterization of UFA C═C positional and geometric isomers in complex samples to explore disease biomarkers and food quality control indicators.